Realized review of research works showed that pathologic behavior of ulcerous abomasitis of calves, its preventive maintenance and treatment, as well as the functional status of liver of heart of sick calves require more thorough analysis in the conditions of the Republic of Belarus. Research results proved the perspective direction of experimental investigation of ulcerous abomasitis of calves by means of ulceration of mucous membranes of fundic portion of fourth stomach y means of derangement of circulatory dynamics of organ portions. The proposed experimental model made it possible to obtain animals with pathology similar to the main clinical, biochemical and morphofunctional parameters with such unprompted pathology. Results of realized experiment on reconstitution of ulcerous abomasitis at 13 calves were presented. Indexes of total clinical blood analysis at different stages of experiment, white blood cell differential, biochemical blood indexes, and indexes of abomasal content of experimental calves were presented

Investigation of dynamics of indexes of carbohydrate and lipid exchange at piglets with various body weight at after weaning period, determination of possibility of their application as energy deficit markers, as well as studying the influence of vitamin and energy additive based on rapeseed oil on the indexes of carbohydrate lipid exchange in blood of piglets and their economic indexes were realized in the conditions of the Republic of Belarus. Research results showed that at the weaning period and after it there were changes in piglet organisms caused by adaptation to new housing conditions and that subsequently led to transformation of energy metabolism. After the weaning period there was stated increasing of level of lipid exchange in the energy supply of adaptation process, that was proved by the increased level of total lipids and triglycerides. Low indexes of carbohydrate and lipid exchange of the group piglets with low growth indicated to the insufficient level of energy supplying, needed for growth and adaptation. The studied vitamin and energy additives based on rapeseed oil corrected the level of carbohydrate and lipid exchange and could be used as a prophylactic agent against energy deficiency conditions of weaning piglets

Investigation of antimicrobial activity of liquid extractions from the following medicinal plants and their mixtures against S. aureus, B. subtilis, E. coli, P. aeruginosa, and P. vulgaris was realized in the conditions of the Republic of Belarus: peat moss (Sphagnum); greater plantain (Plantago major); plumepoppy (Macleaya); common comfrey (Symphytum officinale); speckled alder (Alnus incana). Research results showed that the best results for septic wounds treatment were obtained apter application of mixture of plumepoppy and greater plantain as it showed the acute antimicrobial, anti-inflammatory, and wound-healthing effect. Antimicrobial activity of the analyzed infusion formula revealed in quicker (on 3-6 days in comparison with control) wound cleansing from microorganisms, quick lowering of bacterial content up to 10E3 bodies per 1 g of tissue and in provision of wound healing without inclusion of saprogenous microflora. Anti-inflammatory effect was characterized by quick (by 2 days) cancellation of acute inflammatory response, that was revealed in lowering of neutrophilic leukocytes quantity up to 59,8 +/- 0,49 %; quicker normalization hematologic indexes and early reduction of perifocal edema; weakening of hyperthemia and hyperemia; decreasing of wound secretion without purulence; absence of leaks and leak pouch; normalization of microcirculation in wound. Wound-healthing effect was revealed in increasing of fibroblast number from 7,1 +/- 0,4 % up to 29,4 +/- 0,4 % on the 7th day and in activation proliferate and synthetically function

Investigation of connection of experimental trichophytosis and relations between changes of clinical implications of disease and dimensions of some immunological indexes was realized in the conditions of the Republic of Belarus. At the first stage of the experiment there analyzed 48 guinea pigs (Cavia porcellus) infected with Trichopyton verrucosum. Phagocytic ability of phagocytes and level of circulating antibodies in indirect hemagglutination test were investigated each 5 days before and during disease. The second stage was realized by the example of 2,5-6 months old calves. In course of study there were analyzed blood and blood serum indexes, indexes of nonspecific resistance, as well as some indexes of cell-mediated immune response and antibody mediated immunity. Comparative analysis the following morphological and biochemical blood indexes of clinically healthy calves, calves after trichophytosis, and calves sick with mild, moderate and severe of disease were presented: erythrocytes; hemoglobin; leukocytes; basophils; eosinophiles; rods; microxyphils; lymphocytes; monocytes; ESR; total albumin; albumins; alpha globulins; beta globulins; gamma globulins; carotene; calcium; inorganic phosphorous; alkaline reserve; copper. The realized experiment made it possible to conclude that determination intensity of leukocyte absorption of microconidia of the agent, antibodies against the agent, and specific for cytoplasm fungus, morphological and biochemical blood indexes made it possible to control the development of inflammatory phenomenon in centers of infection, as well as to predict the formation of face, infiltrative and suppurant disease forms

Analysis of efficiency of prebiotics and probiotics and different systems of their application for the focused formation of bacteriocenosis of gastro-intestinal tract of calves was realized in the conditions of the Republic of Belarus. There were used 7 groups of calves: 1 – clinically healthy; 2 – calves with diarrheal syndrome which did not get preparations; 3 – calves were administrated daily with liquid probiotic Bifidoflorin Zhidkij in dose 20 ml in course of 20 days; 4 – calves were administrated daily with prebiotic Biofon with colostral milk or milk in dose 20ml in course of 20 days; 5 – calves were administrated daily with prebiotic Biofon-AIL in dose 20 ml in course 20 days; 6 – calves were administrated with probiotic Bifidoflorin Zhidkij simultaneously with prebiotic Biofon; 7 – calves were administrated with probiotic Bifidoflorin Zhidkij in combination with prebiotic Biofon-AIL. In course of studies there was analyzed the age-specific dynamics of bifidus bacteria, lactic acid bacillus, and Escherichia quantities in calves excrements. Research results showed that application of the proposed prebiotics and probiotics promoted the earlier and active contamination of intestines with obligate anaerobe gram-positive flora with its dominative position in relation to gram-negative nonsporeforming facultative anaerobic flora. At the stage of the second aging immunodeficiency the analyzed preparations rendered the resolving effect. The highest positive indexes were obtained after combined application of probiotic Bifidoflorin Zhidkij with prebiotic Biofon-AIL

Results of studying of therapeutic efficiency of developed in the Republic of Belarus biological preparation of trial series against salmonellosis of animals and birds in the conditions of experimental salmonellosis of calves were presented. Research results showed that dualfold application of multivalent antitoxic serum against salmonellosis of calves, piglets and birds which was caused by association of S. Dublin, S. typhimurium, and S. enteritidis was accompanied by activation of humoral immunity. It was revealed in increasing of agglutinating activity of blood serum against S. enteritidis – in 11,5 times, against S. typhimurium – in 10,5 times, and against S. Dublin – in 10,0 times in 6 days after introduction of infection (4-th day after second application serum). Content of Ig M in blood serum calves which were treated with experimental serum increased in 2,0 times on the 6-th day after introduction of infection. Quantity of Ig G increased in 2,6 times by the 6-th day after introduction of infection. All these changes were typical for the clinical recovery of animals. Treatment efficiency of application of the analyzed serum was 75%

Experimental research on production of antigen preparation for hyperimmunization of bulls from blood of which it was possible to produce an active medical and preventive serum against cattle salmonellosis and pasteurellosis was realized in the conditions of the Republic of Belarus. There were developed four variants of antigen on the basis of the formolated aluminous concentrated vaccine against calf salmonellosis and on the basis of semiliquid aluminum hydroxide vaccine against cattle pesteurellosis. All obtained variants of associated antigen had concentration of hydrogen ions, which was close to the neutral rating; they were also sterile and safe for white mice. Associated antigen which was constructed from vaccines in the ratio of 1:1, 1:2, 1:3, and 2:3 had an ability to raise the protection of immunized experimental guinea pigs in the conditions of controlled introduction of infection with S. dublin 373 and S. tythimurium 371. Pasteurellosis component of associated antigen in 1:1 and 1:2 variants protected against death only 3 out of 5 immunized doves, both in relation to P. multocida strain N 796, and bacteria P. multocida strain N 5264. Vaccine ratio in the associated antigen 1:3 and 2:3 turned to be more immunogenically balanced: all tested doves survived after introduction of infection of broth culture P. multocida of N 796 and N 5264 strains. Experimental results showed that the associated antigen on the basis of vaccines in ratio of 1:3 was sterile, safe, active and suitable for hyperimmunization of bull producers serum against salmonellosis and pasteurellosis of calves, as well as antigen in the variant 2:3, but this ratio increased the expenses

Investigation of influence of complex probiotic preparation Laktimet obtained by means of cultivation of bifidus bacteria and lactic acid bacillus on morphological indexes of pancreatic gland of laying hens was realized in the conditions of the Republic of Belarus. Two groups of laying hens of 121-130 days old were used in the experiment. The experimental hens were given probiotic Laktimet with water in dose of 1 ml/head every day in 4 cycles during 7 days and with 7 days intervals. Total condition, dynamics of body weight, and egg production were estimated. Research results showed that body weight of experimental hens increased on 6,4 % on the 10-th day; on 12,1% - on the 24-th day; on 6% - on the 40-th day; and 7,5% - on the 50-th day of experiment. Pancreas weight of experimental hens increased on 4-9%. Acinus size increased on 11%. Morphological indexes in exocrine pancreatic cells showed that altitude of basal pole increased on 3-7%. Application of probiotic Laktimet rendered positive influence on laying hens that was evident in increasing of their productivity (there was an increasing of live weight on 4-8%, and egg-laying capacity – on 18-33%). The positive dynamics of the majority of morphofunctional indexes of exocrinous and endocrine parts of pancreatic gland proved its significant role in maintenance of general state of laying hens and metabolic processes

Determination of the optimal doses of monocomponents of the developing vaccine against rotavirus infection and cattle colibacillosis was realized in the conditions of the Republic of Belarus by the example of 80pregnant cows with body weight 400-450 kg. Immunological efficiency of the analyzed preparations was estimated in accordance with ascending rate of titres of specific antibodies before and after vaccination. Research results showed that the highest degree of expressed immunologic response to the monocomponents was stated for cows vaccinated in dose 2,5 cm3. Increasing of dose up to 5,0 cm3 did not render substantial influence on gain of specific antibodies level in blood serum of immunized cows. Decreasing of dose up to 1,0 cm3 caused to less intensive biosynthesis of antibodies, excluding animals which were administrated with antigen E. coli K88. In the majority of experimental groups of cows in blood serum there was stated the increasing of specific antibodies level after the second administration of the components. The obtained results proved the necessity of dualfold administration of the developing biological preparation. In course of study there was determined the choice of monocomponent doses of the vaccine. The optimal dose of monocomponent against cattle rotavirus in case of infection titre 7,0 lg 50% tissue cytopathic dose was 2,5-5 cm3 pr animal. The highest titres of the specific anticolibacillosis antibodies for E.coli with concentration of bacterial cells 10E9/ml for K99 and A20 in the conditions of antigen administration in dose 2,5 cm3, and for E.coli with adhesive antigens K88 - 1,0 cm3

Investigation of vitamin supply of cattle affected by fascioles in association with strongylatosis of gastro-intestinal tract was realized in the conditions of the Republic of Belarus by an example of white-and-black 3-8 year old cows. Analysis of blood and blood serum of experimental animals showed that content of B1, B2, and E vitamins was lower in group of animals sick with fascioliasis and strongylatosis of gastro-intestinal tract. There were stated no changes in A and C vitamins content. Content of pyroracemic acid exceeded control indexes and was 22,0 +/- 0,173 mg/l. Content of vitamin A did not change and was 0,011 +/- 0,0012 mg/l for sick animals and 0,013 +/- 0,0043 mg/l at healthy animals. Content of vitamin C was different for sick and healthy animals: 1,685 +/- 0,190 mg/l and 1,93 +/- 0,294 mg/l, respectively. Research results proved that treatment of cattle sick with helminth infestation should be realized in complex. It was proposed to apply premixes and vitamin preparations containing B1, B2, as well as A, E, and C vitamins for the increasing of natural protection and for the creation of unfavourable conditions for disease agents