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Histological and Histochemical investigation of the development of the New -Zealand rabbit’s gastric glands Texto completo
2020
Shehata M.M.Soliman1 | Abdel-Razik H. Abdel-Razik2 | Marwa M. Hussein3 | Omima M. M. Rashad1
The present study aimed to provide a detailed description of the normal development of rabbit stomach and focusing on the histogenesis of gastric glands. In a total, 24 New Zealand White rabbit fetuses were collected at gestational days 21, 25, and 29. The stomachs of the collected fetuses were fixed in 10% neutral buffered formalin and prepared by paraffin technique then stained with Harris's Haematoxylin and Eosin, Masson's Trichrome stain, Orcein, Periodic acid-Schiff, Alcian blue, and Bromophenol blue stains. The results revealed that, at 21stgestational day, the different parts of the stomach including, cardia, fundus and pylorus could be easily distinguished. On 25th developmental day, the gastric mucosal folds were more prominent in the cardia than fundus and pylorus. At 29th developmental day, tunica mucosa and tunica submucosa of the fetal stomach were laid in longitudinally oriented folds known as rugae. The gastric gland in this age became well developed containing well-demarcated oxyntic and peptic cells. In conclusion, the rabbit stomach is completely differentiated during the embryonic life and the gastric glands were functionally active.
Mostrar más [+] Menos [-]Antibiotic resistance profile of Gram-negative bacteria isolated from Lafenwa abattoir effluent and its receiving water (Ogun River) in Abeokuta, Ogun state, Nigeria Texto completo
2020
Samuel N. Akpan | Olubusola A. Odeniyi | Oluwawemimo O. Adebowale | Selim A. Alarape | Olanike K. Adeyemo
Antibiotic resistance profile of Gram-negative bacteria isolated from Lafenwa abattoir effluent and its receiving water (Ogun River) in Abeokuta, Ogun state, Nigeria Texto completo
2020
Samuel N. Akpan | Olubusola A. Odeniyi | Oluwawemimo O. Adebowale | Selim A. Alarape | Olanike K. Adeyemo
Untreated abattoir effluent constitutes potential reservoir for transmission of pathogenic strains of multiple antibiotic-resistant bacteria by pollution of surface and ground water sources. This study was carried out to determine the antibiotic resistance and extended spectrum β-lactamase (ESBL) production profiles of Gram-negative bacteria isolated from effluent collected from Lafenwa municipal abattoir and its receiving surface water, Ogun River, in Abeokuta, Ogun state, Nigeria. Twelve effluent and 18 water samples were collected for this study. Total heterotrophic and coliform counts were estimated, bacterial identification was performed using standard culture-based procedures, whilst antibiotic resistance profiles of isolated bacteria against five antibiotics (ceftazidime, cefpodoxime, cefotaxime, ertapenem and amoxicillin-clavulanate) and detection of ESBLs were done using disk diffusion and double-disc synergy tests. A total of 54 Gram-negative bacteria were isolated, including Salmonella spp. (9), Escherichia coli (15), Klebsiella spp. (7), Shigella spp. (5), Pseudomonas spp. (12) and Enterobacter spp. (6). Both Enterobacteriaceae and Pseudomonas isolates (31% and 66.6%, respectively) were resistant to all selected antibiotics except ertapenem (98% susceptibility). Overall, 77% isolates had multiple antibiotic resistance index (MARI) values, but none of the antibiotic-resistant isolates showed evidence of ESBL production. The presence of multiple antibiotic-resistant isolates in the effluent and receiving water of Lafenwa abattoir suggests a major risk to public health and food safety. Current methods of waste disposal at the abattoir are unacceptable and greatly reduce the qualities of the processed meat and contaminate the environment. There is a need for improved abattoir waste management and water treatment strategies.
Mostrar más [+] Menos [-]Antibiotic resistance profile of Gram-negative bacteria isolated from Lafenwa abattoir effluent and its receiving water (Ogun River) in Abeokuta, Ogun state, Nigeria Texto completo
2020
Akpan, Samuel N.(University of Ibadan Faculty of Veterinary Medicine Department of Veterinary Public Health and Preventive Medicine) | Odeniyi, Olubusola A.(University of Ibadan Faculty of Sciences Department of Microbiology) | Adebowale, Oluwawemimo O.(Federal University of Agriculture College of Veterinary Medicine Department of Veterinary Public Health and Preventative Medicine) | Alarape, Selim A.(University of Ibadan Faculty of Veterinary Medicine Department of Veterinary Public Health and Preventive Medicine) | Adeyemo, Olanike K.(University of Ibadan Faculty of Veterinary Medicine Department of Veterinary Public Health and Preventive Medicine)
Untreated abattoir effluent constitutes potential reservoir for transmission of pathogenic strains of multiple antibiotic-resistant bacteria by pollution of surface and ground water sources. This study was carried out to determine the antibiotic resistance and extended spectrum β-lactamase (ESBL) production profiles of Gram-negative bacteria isolated from effluent collected from Lafenwa municipal abattoir and its receiving surface water, Ogun River, in Abeokuta, Ogun state, Nigeria. Twelve effluent and 18 water samples were collected for this study. Total heterotrophic and coliform counts were estimated, bacterial identification was performed using standard culture-based procedures, whilst antibiotic resistance profiles of isolated bacteria against five antibiotics (ceftazidime, cefpodoxime, cefotaxime, ertapenem and amoxicillin-clavulanate) and detection of ESBLs were done using disk diffusion and double-disc synergy tests. A total of 54 Gram-negative bacteria were isolated, including Salmonella spp. (9), Escherichia coli (15), Klebsiella spp. (7), Shigella spp. (5), Pseudomonas spp. (12) and Enterobacter spp. (6). Both Enterobacteriaceae and Pseudomonas isolates (31% and 66.6%, respectively) were resistant to all selected antibiotics except ertapenem (98% susceptibility). Overall, 77% isolates had multiple antibiotic resistance index (MARI) values, but none of the antibiotic-resistant isolates showed evidence of ESBL production. The presence of multiple antibiotic-resistant isolates in the effluent and receiving water of Lafenwa abattoir suggests a major risk to public health and food safety. Current methods of waste disposal at the abattoir are unacceptable and greatly reduce the qualities of the processed meat and contaminate the environment. There is a need for improved abattoir waste management and water treatment strategies.
Mostrar más [+] Menos [-]Failure of diplodiatoxin to induce diplodiosis in juvenile goats Texto completo
2020
Christo J. Botha | Louis G.J. Ackerman | Mxolisi G. Masango | Luke F. Arnot
Failure of diplodiatoxin to induce diplodiosis in juvenile goats Texto completo
2020
Christo J. Botha | Louis G.J. Ackerman | Mxolisi G. Masango | Luke F. Arnot
Diplodiosis is an important neuromycotoxicosis of ruminants in South Africa when grazing on harvested maize fields in winter. It is believed to be caused by mycotoxin(s) synthesised by Stenocarpella (Diplodia) maydis. Although several metabolites have been isolated from S. maydis culture material, none of these have been administered to ruminants to reproduce the disease. The objectives of this study were to isolate diplodiatoxin and to administer it to juvenile goats. Diplodiatoxin, considered as a major metabolite, was purified from S. maydis-infected maize cultures (Coligny 2007 isolate). Following intravenous administration of 2 mg and 4 mg diplodiatoxin/kg body weight for five consecutive days to two juvenile goats, no clinical signs reminiscent of diplodiosis were observed. Based on previous experimental results and if diplodiatoxin was the causative compound, the dosage regimen employed was seemingly appropriate to induce diplodiosis. In addition, intraruminal administration of 2 mg/kg diplodiatoxin to one goat for three consecutive days also did not induce clinical signs. It appears as if diplodiatoxin alone is not the causative compound. Other metabolites and/or mixtures of diplodiatoxin and other mycotoxins, when available in sufficient quantities, should also be evaluated.
Mostrar más [+] Menos [-]Failure of diplodiatoxin to induce diplodiosis in juvenile goats Texto completo
2020
Botha, Christo J.(University of Pretoria Faculty of Veterinary Science Department of Paraclinical Sciences) | Ackerman, Louis G.J.(University of Pretoria Faculty of Veterinary Science Department of Paraclinical Sciences) | Masango, Mxolisi G.(University of the Witwatersrand Analytics and Institutional Research Unit) | Arnot, Luke F.(University of Pretoria Faculty of Veterinary Science Department of Production Animal Studies)
Diplodiosis is an important neuromycotoxicosis of ruminants in South Africa when grazing on harvested maize fields in winter. It is believed to be caused by mycotoxin(s) synthesised by Stenocarpella (Diplodia) maydis. Although several metabolites have been isolated from S. maydis culture material, none of these have been administered to ruminants to reproduce the disease. The objectives of this study were to isolate diplodiatoxin and to administer it to juvenile goats. Diplodiatoxin, considered as a major metabolite, was purified from S. maydis-infected maize cultures (Coligny 2007 isolate). Following intravenous administration of 2 mg and 4 mg diplodiatoxin/kg body weight for five consecutive days to two juvenile goats, no clinical signs reminiscent of diplodiosis were observed. Based on previous experimental results and if diplodiatoxin was the causative compound, the dosage regimen employed was seemingly appropriate to induce diplodiosis. In addition, intraruminal administration of 2 mg/kg diplodiatoxin to one goat for three consecutive days also did not induce clinical signs. It appears as if diplodiatoxin alone is not the causative compound. Other metabolites and/or mixtures of diplodiatoxin and other mycotoxins, when available in sufficient quantities, should also be evaluated.
Mostrar más [+] Menos [-]A case report of anatomical variation in the left kidney of a Pit Bull dog in Trinidad Texto completo
2020
Mohamed, R.
The current study showed that the right and left kidneys of a Pit Bull dog were normal, the only difference, which appeared in the left kidney, is the bulging of the mid-lateral border with a marked depression in the craniolateral aspect of the left kidney.
Mostrar más [+] Menos [-]Potential risk of some pathogens in table eggs Texto completo
2020
EL-Kholy, A.M. | Saadia H. EL-Shinawy | Hemmat Seliem | Mohamed M.A. Zeinhom
This study was conducted to record the potential risk of some pathogens in table eggs in Beni-Suef city, Egypt. A total of 100 table eggs samples (Farm and Baladi eggs) were randomly collected from poultry farms, markets, supermarkets and groceries in Beni-Suef city, 50 farm eggs samples (each of 3 eggs) and 50 baladi eggs samples (each of 5 eggs) were examined for the presence of coliforms, E.coli, Salmonella, coagulase positive Staphylococcus aureus and Staphylococcal enterotoxins. Isolates were identified by biochemical, serological and molecular (PCR) methods. The obtained results in the present study revealed that (22%) of the examined samples were contaminated with Coliforms. The other identified genera were Citrobacter freundii, Citrobacter diversus, Edwardsiella tarda, Enterobacter spp., Morgonella morganii, Klebsiella oxytoca, Providencea spp., Serratia fonticola and Yersinia intermedia. E.coli (atypical type) was detected in a percentage of 27.27%. True fecal E. coli and Salmonella spp. failed to be detected in any of the whole examined 100 eggs samples. Additionally, Staphylococcus spp. was detected with incidence rate of (13%), out of them, 8 (61.5%) isolates were accounted for coagulase positive Staphylococcus aureus. Enterotoxin profiling revealed that two classical enterotoxin genes (SEA and SED) were detected either singly or in combination. The potential health hazards and the suggested control measures of the isolated strains had been discussed.
Mostrar más [+] Menos [-]Radiologic, Ultrasonic and pathological assessments of locally applied Chitosan on promotion of experimentally induced tibial fracture healing in rats Texto completo
2020
Kotb, M. M. A1 | Ragab, G. A.2 | Fathy, M. Z.2 | Haggag, U.2 | Nesreen M. Safwat3
The objective of the present study was to evaluate the ability of Chitosan to promote induced tibial fracture healing in a rat model. The study was conducted on 14 albino rats divided into two equal groups (seven rats in each group). The first group was considered as a control group. The second group was injected Chitosan solution 0.1 mg/kg into the fracture gap. The progress of healing in each group was evaluated by clinical, radiography, ultrasonography and pathological examinations. The healing process was observed to be superior in the Chitosan group compared to the control one. Chitosan was found to promote healing of injured bone and is suggested to be used in cases of complicated or delayed bone fracture.
Mostrar más [+] Menos [-]Experimental infection of tigerfish (Hydrocynus vittatus) and African sharp tooth catfish (Clarias gariepinus) with Trichinella zimbabwensis Texto completo
2020
Louis J. la Grange | Samson Mukaratirwa
Trichinella zimbabwensis naturally infects a variety of reptilian and wild mammalian hosts in South Africa. Attempts have been made to experimentally infect piranha fish with T. zimbabwensis and T. papuae without success. Tigerfish (Hydrocynus vittatus) and African sharp tooth catfish (Clarias gariepinus) are accomplished predators cohabiting with Nile crocodiles (Crocodylus niloticus) and Nile monitor lizards (Varanus niloticus) in southern Africa and are natural hosts of T. zimbabwensis. To assess the infectivity of T. zimbabwensis to these two hosts, 24 African sharp tooth catfish (mean live weight 581.75 ± 249.71 g) randomly divided into 5 groups were experimentally infected with 1.0 ± 0.34 T. zimbabwensis larvae per gram (lpg) of fish. Forty-one tigerfish (mean live weight 298.6 ± 99.3 g) were randomly divided for three separate trials. An additional 7 tigerfish were assessed for the presence of natural infection as controls. Results showed no adult worms or larvae of T. zimbabwensis in the gastrointestinal tract and body cavities of catfish sacrificed at day 1, 2 and 7 post-infection (p.i.). Two tigerfish from one experimental group yielded 0.1 lpg and 0.02 lpg of muscle tissue at day 26 p.i. and 28 p.i., respectively. No adult worms or larvae were detected in the fish from the remaining groups sacrificed at day 7, 21, 28, 33 and 35 p.i. and from the control group. Results from this study suggest that tigerfish could sustain T. zimbabwensis under specific yet unknown circumstances.
Mostrar más [+] Menos [-]DIFFERENTIAL EXPRESSION OF SECRETED ASPARTYL PROTEASE (SAP8 AND SAP10) GENES AND COMPARATIVE PATHOGENICITY OF Candida albicans GROWN IN VARIOUS ENVIRONMENTAL CONDITIONS Texto completo
2020
Inas K. Rahem | Hayder M. Samaka | Hutheyfa A. Al Salih
This study focusing on the importance of Candida albicans growth conditions on SAP8and SAP10 genes expression, as a member of the Secreted Aspartic Proteases superfamily genesthat play a role in the pathogenicity of C. albicans and the effects of this conditions on thepathogenicity of this bacterium in albino mice.Ten pathogenic isolates of C. albicans weregrown on two different conditions using RPMI1640 medium at 37° C for mimic host conditionand on Sabouraud Dextrose Agar (SDA) at 25° C as in vitro condition. Sets of primers were usedto detect SAP8 and SAP10 genes expression in each condition. Forty BALB/c albino mice wereassigned to groups and inoculated with 1 X 107 cells/mouse of C. albicans grown in the studyconditions as challenge dose. Kidneys, lungs, and liver were collected to study the pathologicalchanges. Data show overexpression of the SAP8 gene in study isolates grown in RPMI1640comparison to the SAP10 gene. Kidneys, liver, and lungs showed pathological lesions at adifferent range of severity, a significant severe lesion in the kidney in mice injected with C.albicans grown in RPMI 1640 medium, while in contrast the significant severe liver and lunglesions were observed in mice injected with C. albicans grown in SDA medium. This studypoints out that the growth condition of C. albicans plays a role in the pathogenicity of thismicroorganism and SAP8 gene related to the infection process in the host.
Mostrar más [+] Menos [-]ANTIBACTERIAL EFFICACY OF ETHANOLIC EXTRACT OF GARLIC (Allium sativum) AND SUMAC (Rhus coriaria) AND ANTIBIOTIC ON Salmonella typhimurium ISOLATED FROM CHICKENS Texto completo
2020
Mahasen A. Khudair | Jenan Nadhim Sadeq | Zahira A. AL-Zuhairi
Antibacterial drug resistance is an increasingly worldwide occurred health problempresented by bacterial-originated defectiveness to the work of a wide-range of antibacterialdrugs. Uncovering the antibacterial effects of ethanolic extract of garlic (Allium sativum) (Glc)and sumac (Rhus coriaria) (Smc) on Salmonella typhimurium isolated from chickens was themain goal of the present study.Fifty samples of intestinal contents of chickens were collectedrandomly from various farms located in Al-Diwaniyah province,All specimens inoculated intoon macconky agar, Salmonella-Shigella agar at 37c for 24-48-hr, also examined on XLD agarand Salmonella CHROME agar Allium sativum( Glc) or Rhus coriaria (Smc )extract, atdifferent concentrations, or antibacterial drugs (control), 10mcg ciprofloxacin (Cip), 30mcgamoxicillin/clavulanic acid (Amc), 10mcg neomycin (N), were employed to test theirantibacterial activities (AAs) against S. typhimurium using agar-gel diffusion tests, Theexperiment included an investigation about of one isolates from origin 6 isolates of S.typhimurium , 6 out of the 50 chicken samples (12%) were culture positive for salmonella typhimurium , Significant (p˂0.05) increases in the AAs against S. typhimurium were shown byGlc or Smc extracts when compared to those from the antibiotics. Moreover, these AA increaseswere revealed to be incremented as the concentrations of those extracts were elevated. Nosignificant (p˃0.05) differences were demonstrated between the AAs of both extracts. Inaddition, Cip, Amc, and N showed AAs against S. typhimurium; however, Cip revealed thestrongest AAs followed by Amc.
Mostrar más [+] Menos [-]EFFECT OF Chlorella MICROALGAE AND GERMINATED BARLEY POWDER ON PERFORMANCE, SOME HEALTH INDICES, AND MEAT HYGIENE PARAMETERS OF COMMON CARP (Cyprinus carpio) Texto completo
2020
Nasreen Mohialddin Abdulrahman | Havan Dwud Sleman | Derin Omer Muhammed Ramzi | Hevar Araz Hama-Salih
This experiment was done to assess the effect of Chlorella, as a source of protein, andgerminated barley powder, as a source of natural prebiotic, on the performance of commoncarp. The experiment was conducted by using one-way ANOVA (analysis of variance) with acompletely randomized design (CRD). Higher significantly in T2 with 5% Chlorella and T5(20% germinated barley). FCR recorded significant differences among treatment as comparedto T5 (20% germinated barley), and the opposite was observed in FER. T5 with 20%germinated barley was differing significantly in each of the spleen somatic and kidneysomatic indices. Adding germinated barley leads to enhanced intestine weight index in T4 andT5. The parameters were hepatosomatic and gill somatic indices, condition factor, fish weightwithout viscera, fish weight without viscera and head, lipids, ash and moisture, organolepticevaluation of mean juiciness, flavor, color, and complete acceptance. We conclude that theuse of Chlorella and germinated barley potentially enhances the growth performance and fish meat quality. We recommend using both plants in earthen ponds to rely on using natural products in fish feeding.
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