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Bovine papillomavirus detection from bovine teats using immunohistochemistry and electronmicroscopy
2005
Bae, Y.C. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea), E-mail: baeyc@nvrqs.go.kr | Lee, C.S. (Chungbuk Livestock and Veterinary Research Institute, Cheongwon, Republic of Korea) | Kang, M.I. (Chonnam National University, Gwangju, Republic of Korea) | Yoon, S.S. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Park, J.W. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Jean, Y.H. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea)
We examined the teats of slaughtered cattles to investigate the prevalence of papilloma and to detect bovine papilloma virus (BPV) from those samples. Among 880 heads, 432 (49.0%) was Holstein and 448 (51.0%) was Korean native cattle. Grossly, out of 432 heads (Holstein), 263 (60.8%) had papilloma lesions on teats. However, out of 448 heads (Korean native cattle), only 33 (7.4%) had papilloma lesions on teats. Immunohistochemically, BPV antigen was detected in 35 heads (22.9%) of 153 (Holstein) which had papilloma lesions. But, in Korean native cattle, BPV antigen was detected in 1 head (3.8%) of 26.
Mostrar más [+] Menos [-]Effects of space allowance on the social behavior of Korean native cattle (Bos taurus coreanae) steers
2005
Han, J.H. (Gyeongsang National University, Jinju, Republic of Korea) | Jeon, J.H. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, D.J. (Gyeongsang National University, Jinju, Republic of Korea) | Chang, H.H. (Gyeongsang National University, Jinju, Republic of Korea) | Koo, J.M. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, Y.K. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, S. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, E.J. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, H.C. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, H.J. (Gyeongsang National University, Jinju, Republic of Korea) | Yeon, S.C. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: scyeon@nongae.gsnu.ac.kr
This study was carried out to find out how space allowance affect the social behavior of Korean native cattle (Bos taurus coreanae) steers. Twelve Korean native cattle (Bos taurus coreanae) steers were used as subjects, each of which was 30-month-old and observation period was from June to July 2003. Five (T1) and seven (T2) steers were allotted to two pens of 5 m×10 m in a building with slate roof and open sides respectively. They were fed at 09:00 h and 16:00 h, twice a day. The behaviors of steers were recorded from 06:00 h to 17:00 h, using two color CCD cameras (Samsung SDC-411, Korea), one B/W CCD cameras (Samsung SBC-340, Korea), one multiplexer (Samsung SDM-081, Korea) and a time lapse VCR (Samsung SRV-30, Korea).
Mostrar más [+] Menos [-]A case of chronic lymphocytic leukemia (CLL) in a Maltese dog
2005
Lee, J.Y. (Konkuk University, Seoul, Republic of Korea) | Hong, E.S. (Konkuk University, Seoul, Republic of Korea) | Kang, B.T. (Konkuk University, Seoul, Republic of Korea) | Jung, D.I. (Konkuk University, Seoul, Republic of Korea) | Park, C. (Konkuk University, Seoul, Republic of Korea) | Park, H.M. (Konkuk University, Seoul, Republic of Korea), E-mail: parkhee@konkuk.ac.kr
An 11-year-old, 3.3 kg, male Maltese dog was referred to Veterinary Teaching Hospital of Konkuk University because of diarrhea and severe anemia. Abnormal physical examination findings included left submandibular lymph node enlargement, pale mucous membrane, cataract, and bloody diarrhea. Results of hematologic examination revealed a marked lymphocytosis resulting in leukocytosis and the markedly increased numbers of small, well-differentiated lymphocytes in the peripheral blood. Serum biochemical abnormalities consisted of elevated AST and ALP, hyperphosphatemia, hypoglycemia, and hypoalbuminemia.
Mostrar más [+] Menos [-]Comparison of sedative effects using intermittent intravenous bolus injection with tiletamine-zolazepam, xylazine-ketamine, and propofol in beagle dogs
2005
Lee, S.H. (Konkuk University, Seoul, Republic of Korea) | Bae, C.S. (Chonnam National University, Gwangju, Republic of Korea) | Lee, B.H. (Konkuk University, Seoul, Republic of Korea) | Chung, B.H. (Konkuk University, Seoul, Republic of Korea), E-mail: chungbh@konkuk.ac.kr
To compare the sedative effects using intermittent intravenous bolus injection with tiletaminezolazepam (n = 5, TZ group), xylazine-ketamine (n = 5, XK group) and propofol (n = 5, PI group), we investigated the changes of hemodynamic (heart rate, arterial pressure), SpO₂, rectal temperature, respiratory rate and pain score during 60 minute sedation and 40 minute recovery period in beagle dogs. The value of rectal temperature was significantly higher in PI groups (p less than 0.05) during recovery period. The value of heart rate was significantly lower in XK group (p less than 0.05) during sedation. The changes of respiratory rate were similar tendency in all groups.
Mostrar más [+] Menos [-]Effects of bone healing capacity by autologous fibrin glue in experimental bone defect dogs
2005
Lee, J.I. (Chonbuk National University, Jeonju, Republic of Korea) | Song, H.N. (Chonbuk National University, Jeonju, Republic of Korea) | Kim, N.S. (Chonbuk National University, Jeonju, Republic of Korea), E-mail: namsoo@chonbuk.ac.kr
This study was investigated of the bone healing capacity by autologous fibrin glue in experimental bone defect dogs. The autologous fibrin glue manufactured just before the experiment was mixed with the concentrated fibrinogen from whole blood of the experimental dog and bovine thrombin. The experimental group was constituted with seven dogs. The experimental osteotomy was performed 5 mm length in bilateral region of proximal diaphyseal fibulae. The defected regions of experimental group were filled with the autologous fibrin glue by duploject. The experimental regions had been radiographed biweekly for 16 weeks to observe new bone formation and union.
Mostrar más [+] Menos [-]Discrimination of Hanwoo from Holstein/black Angus meat by PCR-RFLP of MC1R gene
2005
Kim, T.J. (Chonnam National University, Gwangju, Republic of Korea) | Lee, J.I. (Chonnam National University, Gwangju, Republic of Korea), E-mail: jaeil@chonnam.ac.kr
The melanocortin 1 receptor (MC1R) plays an important role in regulation of melanin pigment synthesis within mammalian melanocytes. Mutations within the gene encoding MC1R have been shown to explain coat color variations within several mammalian species including cattle. To develope a rapid and accurate method for the identification of Hanwoo, we performed a modified PCR-RFLP analysis of MC1R gene using single nucleotide polymorphism (SNP) within MC1R as a target. A size of 538 bp (537 bp for Hanwoo) was amplified by PCR, digested with Hpa Ⅱ, and electrophoresed on a 1.5% agarose gel. A PCR product from Hanwoo showed a single band of 537 bp, whereas two fragments of 328 bp and 210 bp were detected in both Holstein and Black angus.
Mostrar más [+] Menos [-]Juvenile osteosarcoma in the femur of an Alaskan malamute
2005
Jee, H. (Seoul National University, Seoul, Republic of Korea) | Kim, H.W. (Haemaru Animal Referral Hospital, Pundang, Republic of Korea) | Oh, S.Y. (Seoul National University, Seoul, Republic of Korea) | Pakhrin, Bidur (Seoul National University, Seoul, Republic of Korea) | Kim, D.Y. (Seoul National University, Seoul, Republic of Korea), E-mail: daeyong@snu.ac.kr | Choi, J.H. (Haemaru Animal Referral Hospital, Pundang, Republic of Korea)
An 18-month-old male Alaskan malamute was euthanized due to slowly growing mass around the left femur after history of left posterior limb lameness. At necropsy, a firm confluent mass approximately 15cm in diameter was noted surrounding the femur and proximal portion of the tibia/ulna with traumatic ulceration of the overlying skin. On cross sections of the mass, many unencapsulated firm tan coalescing lobules were noted. The neoplasm consisted of closely packed spindle cells with homogenous eosinophilic material (osteoids) between the neoplastic cells. The pulmonary metastasis was confirmed. Based on the gross and histopathologic examinations, the case was diagnosed as juvenile osteosarcoma.
Mostrar más [+] Menos [-]Risk factors for infectious bronchitis virus infection in laying flocks in three provinces of Korea: preliminary results
2005
Pak, S.I. (Kangwon National University, Chunchon, Republic of Korea), E-mail: paksi@kangwon.ac.kr | Song, C.S. (Konkuk University, Seoul, Republic of Korea) | Mo, I.P. (Chungbuk National University, Cheongju, Republic of Korea) | Song, C.Y. (Boram Animal Hospital, Pyungtaek, Republic of Korea) | Kwon, H.M. (Kangwon National University, Chunchon, Republic of Korea) | Yoon, H.J. (Kangwon National University, Chunchon, Republic of Korea) | Son, Y.H. (Konkuk University, Seoul, Republic of Korea)
To analyze and identify selected risk factors for infectious bronchitis virus (IBV) infection in the growing and laying period of laying-hen flocks, a longitudinal field study was conducted with 27 commercial flocks reared in three provinces of Korea during the period from May 2003 to April 2004. Using monitored data for IBV infection status among study flocks we computed the multivariate odds ratios (ORs) and their corresponding confidence intervals (CIs), and population attributable risks (PARs). Multivariate logistic regression showed significant risk increments for: continuous entry of chick (OR=1.9, 95% CI, 0.7-69.1) and operation years of the layer house greater than or equal to 5 years (OR=3.2, 95%CI, 1.6-389.9).
Mostrar más [+] Menos [-]Four cases of hermaphroditism in dogs
2005
Kwon, Y.S. (Chung-Ang University, Seoul, Republic of Korea) | Park, C.H. (Kitasato University, Towada, Japan), E-mail: baku@vmas.kitasato-u.ac.jp | Jang, K.H. (Kyungpook National University, Daegu, Republic of Korea) | Oyamada, Toshifumi (Kitasato University, Towada, Japan) | Yoshikawa, Hiroyasu (Kitasato University, Towada, Japan)
Hermaphroditism was discovered in four dogs at surgery. For the classification of the type of hermaphroditism in each case, gross morphological and histopathological studies were performed. Based on the histopathological studies, two dogs were diagnosed as unilateral true hermaphroditism because they had mixed gonad containing testis and ovary in unilateral gonad. Whereas two dogs were diagnosed as male pseudohermaphroditism because they had only testis and uterus that had endometrial atrophy. After resection, further follow-up of several months revealed no evidence of clinical abnormalities.
Mostrar más [+] Menos [-]Application of oral cyclosporine in a dog with atopic dermatitis
2005
Park, S.J. (Chungnam National University, Daejeon, Republic of Korea), E-mail: parksj@cnu.ac.kr
A 7.1 kg, seven-year old, castrated male, Shih-Tzu with severe pruritus, chronic otitis externa and Malassezia infection was referred to Veterinary Medical Teaching Hospital of Chungnam National University. In local animal hospital, steroid therapy was used to treat uncontrollable pruritus, but the clinical signs were recurrent when steroid therapy was discontinued. On physical examination, generalized alopecia, erythema, papules, severe crust and diffuse lichenification were presented. Tape strip test of skin lesions revealed cocci and Malassezia infections. Based on the result of history, clinical signs and examination described above, canine atopic dermatitis with secondary superficial pyoderma and Malassezia dermatitis was diagnosed.
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