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Inter-laboratory validation of liquid chromatography–tandem mass spectrometry multi-mycotoxin determination in animal feed – method transfer from the reference laboratory to regional laboratories Texto completo
2023
Jedziniak Piotr | Panasiuk Łukasz | Pietruszka Katarzyna | Kolano Krzysztof | Zbytniewski Radosław
The results are presented of the inter-laboratory validation of a liquid chromatography–tandem mass spectrometry method for the determination of eight mycotoxins (aflatoxin B1, deoxynivalenol, fumonisin B1, fumonisin B2, ochratoxin A, toxin T-2, toxin HT-2 and zearalenone) in animal feeds.
Mostrar más [+] Menos [-]Molecular identification of blaTEM and blaCTX-M genes in multidrug-resistant Escherichia coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia Texto completo
2023
Widodo Agus | Lamid Mirni | Effendi Mustofa Helmi | Tyasningsih Wiwiek | Raharjo Dadik | Khairullah Aswin Rafif | Kurniawan Shendy Canadya | Yustinasari Lita Rakhma | Riwu Katty Hendriana Priscilia | Silaen Otto Sahat Martua
Escherichia coli is an opportunistic bacteria that can grow easily, produce toxins, and resist antibiotics. The phenomenon of E. coli developing multidrug resistance is currently the subject of extensive research. The objective of this study was to molecularly identify blaTEM and blaCTX-M genes in multidrug-resistant E. coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia.
Mostrar más [+] Menos [-]Levels of caesium-137 in food of animal origin in Poland Texto completo
2023
Gembal Magdalena | Czerski Paweł | Milczarczyk Ewelina | Warenik-Bany Małgorzata
Radioactive contamination of the environment is one of the greatest threats after a nuclear accident due to released radionuclides. From a radiotoxicological point of view, the most important radionuclide is caesium-137. Formed mainly during nuclear explosions, caesium-137 can persist in the soil for many years, from where it constantly enters the food chain. One of the elements of ensuring food safety is the monitoring of its radioactive contamination, mainly with radioactive caesium isotopes. The aim of the study was to determine the content of caesium-137 in food of animal origin.
Mostrar más [+] Menos [-]Protective effects of N-acetyl-l-cysteine against penconazole-triggered hepatorenal toxicity in adult rats Texto completo
2023
Morgan Ashraf M. | Ogaly Hanan A. | Kamel Shaimaa | Rashad Maha M. | Hassanen Eman I. | Ibrahim Marwa A. | Galal Mona K. | Yassin Aya M. | Dulmani Sharah A. Al | Al-Zahrani Fatimah A.M. | Hussien Ahmed M.
Penconazole (PEN) is a widely applied triazole fungicide. This study sought to define the efficacy of N-acetyl-l-cysteine (NAC) in mitigating PEN-triggered hepatorenal toxicity in rats.
Mostrar más [+] Menos [-]Examination of the haematological profile of pregnant Polish Holstein-Friesian black-and-white cattle in the early stage Texto completo
2023
Wrzecińska Marcjanna | Kowalczyk Alicja | Czerniawska-Piątkowska Ewa | Kordan Władysław | Araujo Jose Pedro
Cattle health and welfare are monitored via the analysis of the haematological profile, and it shows cattle’s ability to adapt to changing environmental conditions, pregnancy and lactation; profile changes also indicate reproductive disorders. The literature lacks reports of the examination of the haematological profile in cows up to the 50th day of pregnancy (dop). Therefore, this research examined that in cows up to this pregnancy stage.
Mostrar más [+] Menos [-]The use of plant extracts and bacteriophages as an alternative therapy approach in combatting bacterial infections: the study of lytic phages and Stevia rebaudiana Texto completo
2023
Stachurska Xymena | Mizielińska Małgorzata | Ordon Magdalena | Nawrotek Paweł
In the light of the problem of antibiotic resistance, the use of combined alternative therapies in combatting bacteria-related disorders has gained popularity. Bacteriophages are one element implemented in new combination therapy. Stevia rebaudiana is known to have antimicrobial activity and regarded as potentially having a synergistic effect with bacteriophages. Therefore, possible interactions of lytic bacteriophages (MS2, T4 and Phi6) with acetone and methanol S. rebaudiana extracts (SRa and SRm) in the bacterial environment were examined.
Mostrar más [+] Menos [-]Droplet digital PCR quantification of selected microRNAs in raw mastitic cow’s milk from the west of Poland Texto completo
2023
Smulski Sebastian | Pszczoła Marcin | Stachowiak Monika | Bilińska Adrianna | Szczerbal Izabela
MicroRNAs (miRNAs), a class of noncoding small RNAs, have been recognised as potential biomarkers of mammary gland conditions, including bovine mastitis diagnosis. The aim of this study was to quantify selected miRNAs in the milk of mastitic cows.
Mostrar más [+] Menos [-]Some aspects of the diagnosis and treatment of eosinophilic granuloma in cats Texto completo
2023
Omelchenko Hanna | Avramenko Natalia | Kulynych Serhii | Petrenko Maksym | Volosovets Vladyslav | Volosovets Natalia | Woźniakowski Grzegorz
Eosinophils represent the most active cells in mammals that show protective and assistive activity in the host immune defence against helminth parasites. These cells are also responsible for the reduction of allergic and inflammatory reactions. The eosinophils play a key role in allergic reactions by secretion of different chemical molecules leading to swelling, lesions and granuloma onset.
Mostrar más [+] Menos [-]The serological and genetic diversity of the Leptospira interrogans Icterohaemorrhagiae serogroup circulating in the UK Texto completo
2023
Arent Zbigniew | Gilmore Colm | Pardyak Laura | Dubniewicz Klaudia | McInerney Barry | Ellis William
The serological and genetic diversity of the Leptospira interrogans Icterohaemorrhagiae serogroup circulating in the UK Texto completo
2023
Arent Zbigniew | Gilmore Colm | Pardyak Laura | Dubniewicz Klaudia | McInerney Barry | Ellis William
Strains of Leptospira interrogans belonging to two very closely related serovars, Icterohaemorrhagiae and Copenhageni, have been associated with disease in mammalian species and are the most frequently reported agents of human leptospirosis. They are considered the most pathogenic serovars and represent more than half of the leptospires encountered in severe human infections.
Mostrar más [+] Menos [-]The serological and genetic diversity of the Icterohaemorrhagiae serogroup circulating in the UK Texto completo
Zbigniew Arent | Colm Gilmore | Laura Pardyak | Klaudia Dubniewicz | Barry McInerney | William Ellis
Introduction: Strains of Leptospira interrogans belonging to two very closely related serovars, Icterohaemorrhagiae andCopenhageni, have been associated with disease in mammalian species and are the most frequently reported agents of humanleptospirosis. They are considered the most pathogenic serovars and represent more than half of the leptospires encountered insevere human infections. Material and Methods: Nineteen such isolates from the United Kingdom – human, domestic andwildlife species – were typed using three monoclonal antibodies (F12 C3, F70 C14 and F70 C24) in an attempt to elucidate theirepidemiology. They were further examined by restriction endonuclease analysis (REA), multiple-locus variable-number tandemrepeat analysis (MLVA) and lic12008 gene sequence analysis. Results: Monoclonal antibody F12 C3, which is highly specificfor Icterohaemorrhagiae and Copenhageni, confirmed that all the strains belonged to these two serovars. Sixteen strains wereidentified as Copenhageni and three as Icterohaemorrhagiae serovar. Only one restriction pattern type was identified, thusconfirming that REA is not able to discriminate between the Icterohaemorrhagiae and Copenhageni serovars. Variable-numbertandem-repeat analysis found three loci with differences in the repeat number, indicating genetic diversity between Britishisolates. Sequences of the lic12008 gene showed that all isolates identified as the Icterohaemorrhagiae serotype have a singlebase insertion, in contrast to the same sequences of the Copenhageni serotype. Conclusion: Copenhageni is the predominantserovar in the Icterohaemorrhagiae serogroup isolated in British Isles. There is a genetic diversity of MLVA patterns of theisolates but no genetic tool used in the study was able to determine serovars. | Leptospira, leptospirosis, Icterohaemorrhagiae, Copenhageni, animal pathogens | 200 | 529-536 | 4
Mostrar más [+] Menos [-]Can the examination of different types of hive samples be a non-invasive method for detection and quantification of viruses in honey bee (Apis mellifera L.) colonies? Texto completo
2023
Čukanová Eliška | Prodělalová Jana | Palíková Miroslava | Kováčová Kristýna | Linhart Petr | Papežíková Ivana
Honey bee viruses have been shown to negatively affect the vigour and longevity of European honey bees (Apis mellifera L). In the present work, beehive materials were tested for their potential to serve as non-invasive samples for honey bee virus detection.
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