A number of studies have measured collagen fibers and collagen
deposition in wound healing process with advances imaging techniques. However, these are performed by complicated methods and need specific tools. In search of the easier ways in routine histopathological laboratory, collagen measurement and staining pattern of wound healing process were observed in wounded skin of Sprague Dawley’s rat by using two different stains which are standard haematoxylin and eosin (H&E) and modified Masson’s
trichrome staining (MT). The comparison between these staining in wounded tissues was made to evaluate the advantages and disadvantages of both staining in wound healing study for 21 days postwounding. Tissues which stained with MT staining was then evaluated its collagen re-organization and density by using polarized light microscope with the aid of image analyzer software. Results showed that tissues stained with standard H&E could not be used to measure and differentiate the collagen deposition which is contradictory to MT staining. Wounded tissue stained with MT staining has showed a clear view of collagen fibers deposition
and re-organisation compared to H&E staining. This finding could validate the using of modified MT staining which leads to accurate histopathological analysis and observation in wound healing study.

This paper reports on the laboratory animal population in the
Laboratory Animal Unit, Veterinary Research Institute (VRI), Ipoh from 2005 to 2010. Laboratory Animal Unit is a complementary unit which serves to supply laboratory animals to all diagnostic and research units in VRI. The objective of this unit is to breed and produce laboratory animals such as rabbits, mice, rats, guinea
pigs and hamsters for research projects as well as diagnostic and biological activities. The unit also supplies laboratory animals to
private agencies, universities and schools. There are 6 species of laboratory animals in VRI namely rabbit (New Zealand Breed), guinea pig (Hartlay Strain), hamster (Golden Syrian), white mice (Swiss Albino), BALB/c mice and Sprague Dawley Rat. Management of the animals includes breeding, fertility, production and disease monitoring. Generally, white mice bred well and had a high population
from 2005 to 2010 compared to the other laboratory animals due to their short gestation period and large litter size. With the higher current demand for laboratory animals, the production of the unit is
expected to increase.

A biogas plant is an anaerobic digester that produces biogas from organic materials such as animal waste, sewage slurry, vegetable waste and others. The Department of Veterinary Services had developed a small-scale biogas plant at a commercial dairy farm
in Semenyih, Selangor. The objectives of this project were to promote green technology and zero waste concepts on animal farming as well as to mitigate adverse effects on the environment due to unsystematic management of animal waste disposal. The biogas produced was used as fuel for biogas lamp, biogas stove, biogas water heater, biogas rice cooker and 1 kW biogas generator. The generator was connected to the distribution board in order to supply electricity to a few switch sockets and lighting system for farm use. A biogas plant in an animal farm is one of the green technology applications because it not only produces renewable gas and generates
electricity but also minimises greenhouse gas emissions and environmental pollution. Furthermore, the residual solid waste
produced at the end of the process can be dried to be used as organic fertiliser.

Rodents particularly those belonging to the Muridae family in Malaysia have been well studied because of their medical and economic importance. Much of the work on rodents has been focusing on the identification of endo and ecto parasites. Parasites in rats (Murids) particularly helminthes belonging to the Nematoda family have been described by many workers for more than a century. This
paper is an attempt to compile 50 papers on rodent nematodes that has been published in various scientific journals over the last
100 years in Malaysia. It is hoped that this literature overview on rodent nematodes will come useful as a reference material for the budding parasitologist and biology scientist.

Mite Liponyssoides sanguineus and louse Polyplax spinulosa are reported ITomrat (Rattus norvegicus) for the first time from Puducherry.

The experimental trial of six weeks was undertaken on 90 broiler chicks divided into three groups. Control (To) group was fed standard broiler diet and T1 and T2 groups were provided same broiler diet supplemented with 300 and 500 mg of Spirulina per kg feed, respectively. Mean live body weight of six weeks of the experiment and live weight at the end of experiment were found to be significantly (P0.05) higher in Spirulina supplemented T1 and T2 groups of broilers than that of control (To) group. Comparatively better mean weekly weight gain and feed efficiency were also observed in Spirulina supplemented groups (T1 and T2)with decreased feed consumption as compared to control (To) group of broilers.

Melioidosis usually results in chronic debilities that reduce the
productivity of animals and condemnation of carcasses in abattoir. Melioidosis is reemerging among animals and humans, and anecdotal reports suggest an increase in disease observation. This study described the seroprevalence of melioidosis in livestock based on the data obtained from the Department of Veterinary Services, Putrajaya and the Veterinary Research Institute, Ipoh. The data were summarized according to animal species, state, and year. The seroprevalence rate in animals was 7.6, 48.2, 2.6, 13.6 and 3.6% in cattle, buffaloes, goats, sheep and pigs respectively. The
seroprevalence of the disease varies in different states of the federation. For all species, the seroprevalence vary between
2.6% and 48.2%. The seroprevalence over the years increased from 4.2% in 2000 to 12.0% in 2003 after which it varies between
the period 2004- 2007 and apparently declined between 2007 and 2009.

A total of thirty snakehead fishes Channa micropeltes (Cuvier, 1831)
were collected at Tasik Kenyir, Malaysia. Muscle, liver, intestine and kidney tissues were removed from each fish and the intestine was opened to reveal cestodes. The cestodes were identified on the basis
of their morphological characteristics. After staining, drawing by using Camera Lucida, measurement by using advanced microscope and using Scanning Electron Microscope (SEM), the results showed that
the cestode was Senga parva (Fernando and Fertado, 1964). It differed from other species of genus Senga, parasitising fish genus Channa, by the size of the strobila, scolex, apical disc, hooks, number of hooks, testes and some other characteristics.

Rice straw and oil palm empty fruit bunch were treated with urea
and commercial effective microorganisms to study their ability to breakdown fibres, improved in protein and energy values. In rice straw, the crude protein and crude fibre treated with urea and urea + effective microorganisms showed significant differences from the untreated control. The neutral detergent fibre and nitrogen free
extract values in the rice straw treatment did not show significant differences. Metabolizable energy values decreased with the treatment groups compared to the control. The results of keeping the treated rice straw in anaerobic plastic bags up to 30 days showed no significant breakdown of fibre components in the control and urea
treated groups. But there is a significant dropped in the neutral detergent fibre in urea + effective microorganisms treatment.
In oil palm empty fruit bunch, there were no significant differences seen in the crude protein, crude fibre, acid detergent fibre, neutral detergent fibre, nitrogen free extract and metabolisable energy values. Storage showed decreased in energy values in all treated groups. The addition of urea and microbes showed improved protein level and in the reduction of the fibre components in rice straw only. Keywords: rice straw, oil palm empty fruit bunch, urea, effective microorganisms, crude fibre, acid detergent fibre, neutral
detergent fibre, metabolisable energy.