Objective: The purpose of the paper was to monitor the disease incidence in farm and wild animals in some areas of Kazakhstan, which are most susceptible to leptospirosis, and the typifi¬cation of isolated pathogens, carried out under the scientific and technical program "Studying the epizootological characteristics of the country territory on particularly dangerous diseases and developing veterinary and sanitary measures to improve their effectiveness" in 2021–2023. Materials and Methods: The material included the reports of veterinary laboratories on leptospi¬rosis in recent years, as well as laboratory tests on samples carried out at the "SANA" research and development enterprise. During this period, 6,701 serum samples from farm animals and 86,651 serum samples from rodents were tested by enzyme-linked immunosorbent assay. Results: The serological results showed antibody titers in the blood of 6.32% of cattle, 5.4% of sheep, 4.2% of horses, and 1.8% of pigs. The highest number of positive samples were found in Turkestan (12.3%), Almaty (11.7%), and Kyzylorda (11.4%) regions. Infection in rodents was lower and ranged from 0.34% to 0.07% during these years. The population of leptospira-causing diseases of animals on the territory of the country is represented by 8 serogroups. Studies in 2022 on the detection of pathogenic leptospires by polymerase chain reaction in 350 samples of blood serum from animals and 350 samples of biomaterial from rodents from different regions of Kazakhstan were negative. Conclusion: Studies conducted as part of this work will help reduce the incidence of disease among the population and animals in Kazakhstan. [J Adv Vet Anim Res 2024; 11(2.000): 439-448]

Objective: This study aimed to detect Toxocara cati in cats by microscopic and molecular analysis using PCR, sequencing, and phylogenetic analysis. Materials and Methods: Randomly selected 200 cat feces samples were taken from various private veterinarian clinics in Baghdad. To identify eggs of T. cati by the flotation method, DNA from 100 cat feces was extracted, and one pair of ITS2 region-specific primers was used for polymerase chain reaction, followed by sequencing. Results: Toxocara cati infection rate was found to be 23 out of 100 fecal samples using PCR. Ten DNA product sequence data studies showed 98%–100% similarity to the 5.8S ribosomal RNA gene sequences found in the Gene Bank. The study incidence showed that the overall infection rate by microscopic examination was 23%, with no significant difference between stray cats (27%), and domestic cats (19%). After studying the effect of several epidemiological parameters on the infec¬tion rate, it was found that the infection rates of stray and domestic cats were higher in kittens under six months of age, at 46.1% and 27%, respectively, whereas rates were lower for the adult than six months was 11.5% of domestic cats and 14.7% of stray cats. The percentage of stray and domestic male cats that were registered was 35.5%, whereas the female cats registered were 20.6% and 17.5%, respectively. Conclusion: Cats are significant clinical reservoirs for zoonotic parasites. In Iraq, Baghdad has a high incidence of T. cati detections. Compared to conventional methods, PCR is thought to be a more sensitive, accurate diagnostic procedure that confirms the species' identity. [J Adv Vet Anim Res 2024; 11(2.000): 392-397]

Objective: There is still much to be discovered regarding the etiopathogenesis and management of polycystic ovarian syndrome (PCOS). Materials and Methods: Four groups of female Wister-Albino rats were established, each with a normal estrous cycle: control, D ( + ) galactose (D-galactose), Lepidium sativum (L. sativum), and prepared secondary antibody (Ab2). Serum samples were collected, and histopathological examination was performed on ovaries and spleen tissues. Immunoreactive anti-ovarian antibody (AOA) quantities were determined using a modified antigen-based ELISA procedure. ELISA assay kits were used to quantify FSH, LH, and estradiol 17 β concentrations. Results: The study found that AOA concentration in undiluted samples was significantly higher in the second and fourth weeks after PCOS induction by D-galactose (p < 0.001). However, antibody index% and titer elevated in the D-galactose group. L. sativum's late efficacy was observed in the fourth week, while the concentration of undiluted samples in the D-galactose + Ab2 group lowered (p < 0.001). Higher basal FSH and LH levels and lower estrogen levels are associated with PCOS development. L. sativum's immunomodulatory properties may contribute to this associa¬tion. Estradiol-17ß concentrations increased in D-galactose + L. sativum and D-galactose + Ab2 groups, respectively. Conclusion: Careful extrapolation of experimental models is crucial for clinical applications, as technical advancements make Ab2 production easier. Further study is needed to fully understand its potential in immunotherapy. [J Adv Vet Anim Res 2024; 11(2.000): 418-428]

Objective: Research has demonstrated that Leptospermum scoparium possesses various thera¬peutic benefits. This study set out to determine whether or not L. scoparium extracts had any effect on the ability of HepG2 and MCF-7 breast cancer cells to survive. Materials and Methods: The antiproliferative activity of L. scoparium extracts was explored using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays. The most active fraction was selected to investigate its effects on apoptosis induction using flow cytometry and quantitative real-time polymerase chain reaction. The constituents of this fraction were characterized using GC-MS analysis. Results: Research demonstrated that the chloroform fraction of L. scoparium (LSCF) significantly impacted the HepG2 and MCF-7 cancer cell lines. Treatment with LSCF led to a notable rise in both early and late apoptotic cells. Furthermore, there was an upregulation in the mRNA levels of P53, Bax, and caspases, while the expression of Bcl-2 mRNA saw a decrease. The analysis of LSCF revealed the primary components to be cis-calamenene, beta-eudesmol, cyclododecane, and alpha-muurolene. Conclusion: The study showed the promising antiproliferative activity of L. scoparium, suggesting its potential application for cancer treatment. [J Adv Vet Anim Res 2024; 11(2.000): 237-246]

Objective: This study aims to develop formulas for estimating tracheal diameter and length in dogs using easily measurable anatomical parameters. Materials and Methods: The samples consisted of 20 dogs of various breeds, comprising 10 males and 10 females, sourced from cadavers. The measured parameters included occipital tuberosity to tail base (OT), eye angle to ear tragus, nose to ear tragus, inner vertical diameters (IVD), and tracheal length (TL). The study conducted correlation and linear regression analyses, and subsequently, the formulated models underwent validation using 16 live dogs. The results were compared to radiographic measurements. Results: Linear regression recommended formulas based on OT, resulting in IVD (mm) = 0.203 × OT – 3.724 (r2 = 0.608, p < 0.001) and TL (cm) = 0.346 × OT–3.773 (r2 = 0.837, p < 0.001). The predicted tracheal diameter and length from formulas were slightly smaller than radiographic measurements (IVD = 2.76 ± 1.85 mm, p < 0.0001 and TL = 2.07 ± 1.81 cm, p < 0.0001). Conclusion: These formulas offer a practical way to estimate tracheal dimensions in live dogs, facilitating the selection of suitable endotracheal tube sizes and insertion depth. Further studies with larger sample sizes and consistent measurement methods can enhance the accuracy of these findings. [J Adv Vet Anim Res 2024; 11(2.000): 323-329]

Objective: The present research was conducted to evaluate the negative effects of nicotine powder on the blood physiology, and biochemical and histological alterations of Labeo rohita. Materials and Methods: Fish were divided into four groups (1–4). Fish groups 2, 3, and 4 were exposed to different concentrations of nicotine, such as 0.75, 1.25, and 1.75 mg/l, while group 1 acted as a control. To find out the long-term impact of nicotine on body physiology, we conducted a 42-day experiment. After the completion of the experiment, hematology, biochemical assays, and histology were done. Results: Results revealed a considerable increase in HGB, red blood cells, WBCs, hematocrit, mean corpuscular volume, red cell distribution width -SD, procalcitonin, neutrophils, lymphocytes, monocytes, triglycerides, total cholesterol, low-density lipoprotein, very low-density lipoprotein, alanine transaminase, aspartate aminotransferase, globulin, thyroid stimulating hormone, BUN, creatinine, and blood glucose levels, whereas mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, RDW, platelet, high-density lipoprotein, albumin, total proteins, and T3 levels were significantly (p ≤ 0.05) decreased in exposed fish as compared to control group fish. Histological alterations showed that exposure to smokeless nicotine causes deleterious and degenerative effects in the liver, kidney, and gills of exposed fish. Conclusion: Nicotine administration in fish results in adverse effects on different biochemical and hematological parameters and causes histological alterations in some vital organs of exposed fish. [J Adv Vet Anim Res 2024; 11(2.000): 463-473]

Objectives: Current research aimed to compare the effects of fungal and bacterial phytase with or without citric acid (CA) on growth performance, serum mineral profile, bone quality, and nutrient retention in birds given non-phytate phosphorus (nPP)-deficient diets. Materials and Methods: A total of 216 Indian River broiler chicks were disturbed into six groups, namely, i) positive control (PC), ii) negative control (NC) contained 0.2% lower nPP than that in the PC diet, iii) NC + fungal phytase (Aspergillus niger), iv) NC + fungal phytase with 2% CA, v) NC + bacterial phytase (Escherichia coli), and vi) NC + bacterial phytase with 2% CA. Results: Compared to the PC group, the NC group showed poor performance, serum phospho¬rus (P) content, P retention, and bone quality. However, with the inclusion of phytase, all these phenomena were improved. The addition of bacterial phytase showed better values compared with fungal phytase. The main effects of phytase were significant for the feed conversion ratio (FCR), metabolizable energy conversion ratio (MECR), and P retention. The addition of CA, either with fungal or bacterial phytases, did not show considerable beneficial effects on overall performance. However, the main effects of CA were significant on the FCR, MECR, and crude protein conversion ratio. Conclusion: Incorporating bacterial and fungal phytase into low-nPP diets enhanced the broiler's performance. The effects of bacterial phytase were more apparent than those of fungal phytase. However, the efficacy of phytase based on the source might relate to dose, and other factors need further investigation. [J Adv Vet Anim Res 2024; 11(2.000): 376-383]

Objective: This study aims to determine the effect of using multivitamins in different estrus synchronization hormone protocols on estrus response, estrus onset, estrus duration, estrus intensity, and pregnancy rate in swamp buffalo. Materials and Methods: This study used 30 post-partum adult buffalo, with three estrus synchronization methods treated: 1. Conventional plus Prostaglandin F2 α (PGF2α-PGF2α + multivitamin)-AI; 2. Co-synch plus Gonadotropin-releasing hormone (GnRH-PGF2α + multivitamin)-AI; 3. Combination of hormone plus (Estrogen-Progesterone-PGF2α + multivitamin)-AI. Research variables include estrus response, estrus onset, estrus duration, estrus intensity, and pregnancy rate. Data were analyzed using the chi-square test using the Statistical Package for the Social Sciences 23.0 program. Results: The results showed that the use of multivitamins in different estrus synchronization hormone protocols resulted in an estrous response reaching 100%. The onset of estrus in the three treatments [Treatment-1 (T1); Treatment-2 (T2); Treatment-3 (T3)] was 25.8; 27.6; 23.9 h, estrus duration: 21.0; 21.6; 21.92 h, estrus intensity: 25.8; 27.6; 32.6 h, and the pregnancy rate for buffalo reaches 80%. Conclusion: Based on the results of this study, the use of multivitamins in different estrus synchronization hormone protocols is effective in optimizing the swamp buffalo's estrus response; the estrus duration is longer, the estrus onset is faster, and the estrus intensity is higher. It can even optimize the increase in swamp buffalo pregnancy rates. [J Adv Vet Anim Res 2024; 11(3.000): 729-737]

Reverse zoonosis or zooanthroponosis is the transfer of pathogens from humans to animals. Although less studied than zoonotic diseases, this phenomenon poses significant risks to both animal and public health. The increasing human-animal interactions driven by urbanization, globalization, and environmental changes have exacerbated the occurrence of reverse zoonosis. This review evaluated the global impact and transmission patterns of reverse zoonosis, highlighting the anthropogenic and intrinsic factors contributing to its emergence. The study performed a systematic review and included 91 scientific articles published from 2000 to 2022, covering viral, bacterial, parasitic, fungal, and protozoal reverse zoonoses. This study indicated that viral infections, particularly respiratory viruses such as severe acute respiratory syndrome coronavirus-2 and influenza, have the highest incidence of reverse zoonosis, followed by bacterial infections like tuberculosis and methicillin-resistant Staphylococcus aureus. The United States, India, and Hong Kong are among the most reported regions for reverse zoonotic events. Major risk factors identified include environmental degradation, climate change, antimicrobial resistance, and global wildlife trade. The review underscores the need for enhanced surveillance systems, interdisciplinary collaboration, and stringent regulations on wildlife trade and animal husbandry practices to mitigate the risks associated with reverse zoonosis. Understanding the dynamics of human-animal pathogen transmission is crucial for developing not only effective but also sustainable strategies to protect animal populations as well as public health from emerging infectious diseases. [J Adv Vet Anim Res 2024; 11(3.000): 601-617]

Objective: The evaluation of frozen semen quality is an essential aspect in determining male fertility for artificial insemination programs. This study aims to evaluate the characteristics of Friesian Holstein (FH) bull-frozen semen in different classes (proven and unproven) based on protein profiling and molecular evaluation. Materials and Methods: This study used frozen semen straws from FH bulls selected according to criteria for proven (6 individuals) and unproven (6 individuals) bulls produced by the Singosari AI Center (AIC). Sperm motility parameters were assessed using Computer Assisted Semen Analysis (CASA Supervision®, Germany), while sperm viability and abnormality were evaluated through eosin-nigrosin staining under a microscope at 400´ magnifications. The integrity of the sperm plasma membrane was determined using the hypoosmotic swelling test, and acrosome integrity was analyzed using the fluorescein isothiocyanate PNA-propidium iodide staining method. Protamine deficiency was quantified using Chromomycin A3 fluorescence staining, while DNA fragmentation was assessed using the acridine orange technique. Results: The findings demonstrated that there were no statistically significant differences (p > 0.05) in the assessed parameters of frozen semen quality between FH-proven and unproven bulls. Furthermore, in FH-proven bulls, a negative correlation was observed between protamine deficiency and acrosome integrity (r = −0.528) and between protamine deficiency and sperm DNA fragmentation (r = −0.467). The parameters of protamine deficiency in unproven bulls exhibited a positive correlation with sperm progressive motility. Conclusion: The frozen semen quality of FH bulls in different classes (proven and unproven) was found to be equally good. Molecular-based analysis allows for a more accurate determination of semen quality. These findings are significant for bull breeding stations when comprehensively evaluating semen quality. [J Adv Vet Anim Res 2024; 11(3.000): 796-802]