This study aimed to study the effect of antibiotic on phospholipase production of Staphylococcus aureus isolated from preputium of Aceh cattle. The parameters measured in this study were PzI of Staphylococcus aureus without any treatment, and PzI of S. aureus after being given antibiotics. The experiment was carried out by modifying the Samaranayake method on egg yolk agar media, incubated at 37 ° C for 48 hours, and the precipitation lines formed was measured. The antibiotics used were tetracycline, oxytetracycline and fosfomycin with concentrations of 10%, 20% and 30% (mg / mL, respectively). The results showed that phospholipase production of S. aureus isolated from preputium of Aceh cattle was suppressed, antibiotics were able to inhibit phospholipase production. However, the enzyme was still produced in positive category, with PzI = 0.287, the highest result was found in 30% tetracycline activity, which is equal to PzI = 0.341 and the lowest was in 10% fosfomycin which is equal to PzI = 0.332

The 21st Supply Deposit of the Brazilian Army (21st DSup), located in the city of São Paulo provides food raw materials for 16000 meals daily, and frozen fish is among the foodstuffs distributed. The objective of this study was to evaluate the good practices of seafood storage in the 21st DSup, identify issues of non-compliance that compromise food quality, and propose solutions. The system was evaluated, applying a checklist (RDC 275/2002) to evaluate the percentage of requirements in compliance with good practices. The 21st DSup was classified in Group 3, with 41% of items in conformance (< 50%), and we found that there is no hygienic-sanitary self-control program for storing cold seafood and other food supplies. The cold stores’ temperatures are not able to maintain products within the required standards of conservation. The seafood cold storage protocol of the 21st DSup does not guarantee temperature conformity. It is necessary to implement a hygienic-sanitary self-control program for food supply storage, which should begin with the development of a food safety culture.

The central region of Brazil is known to be an endemic area for canine ehrlichiosis. Therefore, this study aims to determine the prevalence rates of E. canis infection in dogs and in Rhipicephalus sanguineus ticks collected from the dogs and their home environments. Serum samples and genomic DNA from the blood of 20 dogs and 299 ticks were analyzed by IFA and PCR assays in order to detect Ehrlichia canis antibodies and DNA. Nine (45%) of the 20 dogs were seropositive for E. canis, with titers ranging from 80 to 10240, and 6 dogs (30%) were positive for Ehrlichia spp. by PCR. Five free-living ticks were positive (2.89%, 95% confidence interval: 0.94-6.62%), as were six ticks attached to dogs (4.76%; 95% CI: 1.77-10.0%). The two groups showed a similar infection rate (P=0.395). Partial dsb DNA sequences of two samples from ticks were identical to each other and 100% (350/350 nucleotides) were identical to E. canis. Despite the high serological and molecular rates of canine ehrlichiosis in Cuiabá, the prevalence among infected ticks was lower than that found among dogs. However, adult ticks may remain infective much longer to ensure their infestation and infection of susceptible dogs.

Canine rangeliosis is an extravascular hemolytic disease caused by the protozoan Rangelia vitalii, which is transmitted by ticks of the species Amblyomma aureolatum. Te most common clinical signs are apathy, hyperthermia and spontaneous bleeding. Anemia and thrombocytopenia are the most common hematological fndings. Tis work reports a clinical case of canine Rangeliosis treated at a private veterinary hospital, in São Paulo city in 2017. A dog was treated at a veterinary hospital in the north of São Paulo, with progressive weight loss, apathy and tail injury. Anemia and thrombocytopenia were observed on the hemogram. Rangelia vitalii DNA was detected in animal blood by real-time PCR (qPCR). In addition to the supportive treatment, doxycycline and subcutaneous imidocarb applications were used. Te sample collected afer treatment with the antibiotic continued to present protozoal DNA. Te disease should be considered as a diferential diagnosis and there is a great need for further studies about the therapy used.

Mycoplasma hyopneumoniae is the causative agent of enzootic pneumonia (EP), a disease that is highly prevalent and globally distributed, causing significant economic losses to the swine industry. Disease progression is characterized by reduced feed conversion and the development of lung lesions. Considering the limited information about the epidemiology of EP in Southern Brazil, the main objective of this study was to determine the occurrence of M. hyopneumoniae in swine lung samples and to evaluate the scores of lung lesions caused by local strains. A total of 120 samples was randomly collected and processed. DNA was extracted from lung tissue to perform nested-PCR and lungs were inspected to evaluate the presence of the pneumonia-like gross lesions of M. hyopneumoniae. The results showed 95.8% positive samples, while the lung lesion score analysis showed suggestive lesions in 60% of samples. The detection of positive samples in nested-PCR was associated with the presence of pneumonia-like gross lesions (P < 0.01). The results demonstrate a high occurrence of EP in slaughter pigs from southern Brazil.

The present study evaluated Brangus cows treated with single doses of follicle stimulating hormone (FSH) subjected to follicular aspiration after 24 h to assess oocyte recovery, in vitro fertilization and pregnancy rate. Follicles exceeding 3 millimeters in diameter were aspirated, 200 mg of FSH was administered 2 days later, and a new ovum pickup was performed 24 h afterward. These methods were performed 3 times every 3 days. In control, follicular aspirations occurred at intervals of 1-week without FSH administration o. The aspirated oocytes were evaluated, submitted to in vitro fertilization and the embryos were transferred to the recipients. The average recovery of oocytes was higher (p<0.05) in control cows (12.4±1.8) than in treated cows (9.4±1.3). There was no difference (p>0.05) in the mean percentage of viable oocytes (52.0±3.9 and 62.7±4.7%) or the mean percentage of embryos (41.4±4.8 and 41.5±4.2%) among control and treated cows, respectively. The mean percentage of pregnancy did not differ (p>0.05) for control cows (43.8±2.7%), and treated cows (40.9±6.8%). In conclusion, FSH treatment did not improve oocyte recovery, in vitro fertilization, and pregnancy percentage. However, there is possibility of several consecutive ovum pickup every t3 days, concentrating the in vitro fertilization and the pregnancy percentage.

The objective of this study was to evaluate the in vivo predatory viability of the nematophagous fungus, Duddingtonia flagrans, after storage (36 months) and refrigeration (2-8 °C). This viability was evaluated using the infective larvae of gastrointestinal nematodes of sheep in the Northeastern semi-arid region of Brazil. Sixteen Santa Inês sheep with negative counting of eggs per gram of feces (EPG) were divided into four experimental groups, each group comprised of four animals. The pellets were administered at the dose of 3 g/10 kg of live weight (20% fungal micelyum), and a single administration was performed for each animal. Group I was administered pellets that had been stored for 36 months; Group II, freshly produced pellets; Group III, freshly produced pellets that did not contain fungi; and Group IV, pellets were not administered, and this was the control group. Feces were collected for 5 days, every 24 h for analysis. There was a significant decrease in the number of infective larvae of sheep nematodes that received D. flagrans pellets in a sodium alginate matrix, 82% was observed for Group I and 71% for Group II, compared to the control group. It is therefore concluded that the fungus, D. flagrans, pelleted in sodium alginate matrix after 36 months of storage at 2-8 °C, showed efficacy in reducing the number of infective larvae of gastrointestinal nematodes of sheep.

Clove oil is distilled from clove flowers (Syzigium aromaticum) and characterized by physical properties and GC-MS. Distillation was carried out by steam distillation method for 8 hours. Analysis of clove oil was carried out by GC-MS method. The distillation results obtained 163 mL of clove oil with physical properties that meet the requirements of SNI 06-4267-1996. The results of the analysis using GC-MS showed that clove oil contained 6 components, namely, Eugenol 88.20%, Alpha Copaene 0.49%, Caryophyllene 3.77%, Alpha Humulene 0.48%, Eugenyl acetate 6.76%, and Caryophyllene Oxide 0.30%. Another fact shows that clove flower oil has anthelmintic activity.

Infection that occurs in Indonesia has increased more significantly than before, compared to the increasing bacterial multidrug resistance (MDR) as the cause of infection. A study conducted in 5 hospitals in Indonesia in 2013 showed that the prevalence rate of extended-spectrum β-lactamase (ESBL)-producing bacteria reached 32-68%. The objective of this study is to detect the prevalence and resistence pattern of ESBL-producing Escherichia coli and Klebsiella pneumoniae in Dr. Zainoel Abidin General Hospital, Banda Aceh. This study was conducted from 1 September 2016 to 31 December 2016. Specimen types included in this study were blood, sputum, urine, pus, mucosal swab, and another body fluids sample. The sampling method in this study was total sampling that is all clinical specimen examined in Clinical Microbiology Laboratory. Isolation and identification ESBL-producing bacteria was performed by VITEK-2 machine (Biomerieux). The result of this study is that a total 122 E. coli and K. pneumoniae were isolated. That consisted of 48 (39%) E. coli isolates and 74 (61%) K. pneumoniae isolates. From 48 E. coli isolates it was found out that 41 (85%) had ESBL phenotypes and from 74 K. pneumoniae isolates it was found out that 59 (80%) had ESBL phenotypes. In total, 100 (82%) isolates from 122 isolates had ESBL phenotypes. Distribution of ESBL-producing E. coli and K. pneumoniae based on sample was 24 (89%) isolates from the total of 27 urine isolates, 18 (95%) isolates from the total of 19 blood isolates, 28 (78%) isolates from the total of 36 sputum isolates, and 30 (75%) isolates from the total of 40 pus isolates. Antibiotic sensitivity pattern of the E. coli and K. pneumoniae isolates had high sensitivity to amycasin dan meropenem which was above 89%. Meanwhile, it also had sensitivity to Fosfomycin and Piperacyclin-Tazobactam by 80% and 77% respectively. Another antibiotic was less effective

The presence of lactic acid bacteria (LAB) and histamine in foodstuffs indicate the level of deterioration in the quality of food and cause poisoning. Sie Balu is the Acehnese dried meat preserved by the addition of salt, acid and dried, but the long processing and drying it under the sun can cause microbial contamination in meat products. Irradiation can eliminate bacteria in foodstuffs. This study aimed to determine the amount of LAB and histamine levels of Sie Balu after irradiation doses of 5, 7 and 9 kGy and stored 2 to 4 months. Sie Balu was made of fresh beef 5 kg, dried in the sun to dry, vacuumed and irradiated with gamma rays. The samples for LAB determination cultured in MRS agar and incubated at 37°C for 24 hours. The number of colonies was counted using Total Plate Count. The histamine level of Sie Balu conducted by ELISA. Irradiation did not significantly (P0.05) affect the amount of LAB, but the shelf life significantly (P0.05) affected the amount of LAB in Sie Balu. Extending the shelf life up to 4 months can increase the amount of LAB. Irradiation dose and shelf life had no effect on histamine levels of Sie Balu (P0.05). This study concluded that irradiated Sie Balu cannot be stored for more than two months.