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MUTANT OF ALOCAL ISOLATE OF LACTOBACILLUS PLANTARUMBY UV LIGHTIN COMPARISION WITH ASTANDARD STRAIN THE EXTENT OF ITSABILITY TO LOWER CHOLESTEROL LEVELS1 Texto completo
2016
Sinan J. Abdul-Abbas | Aswan H. Aboud Al-bayyar | Adnan E. Al-Badran
Local strain bacteria of Lactobacillus plantarum IRQ12 isolated from cucumbersativuspickle registered in (GenBank LN871447) and another standard strain, IRQ12strain was mutated by UV light (254 nm) for (50 cm) distance for different periods 20,25, 30, 35 and 60 second, RAPD – PCR test was done to investigate the effect of UVlight onL.plantarum genes and determine the genetic distant. The ability ofstandard andlocal mutated bacteria to decrease the amount of cholesterol was determined in MRSbroth–EY mediawhich was (4.6 mg/ml), the cholesterol amount was decreased at 25swhich was0.35 mg/ml in a decreased percentage 92.3%, followed by 60s and 35s whichwere 0.43and 0.45mg/ml in a decreased percentage 90.6% and 90.2%, respectively.
Mostrar más [+] Menos [-]EFFECT OF PARITY NUMBER ON SOME CHEMICAL COLOSTRUM CONSTITUENTS THROUGH COLOSTRAL DAY AMONG EWES Texto completo
2016
Amel mustafa Kamil
MORPHOLOGICAL STUDY OF AIR SACS (Sacci pneumatici) IN WHITE CHEEKED BULBUL(Pycnonotus leucotis) Texto completo
2016
Shaymaa K. Jaifar | Alaa A. Sawad
The study was designed to observed the anatomical features of the air sacs (Sacci pneumatici)inWhite Cheeked Bulbul (Pycnonotus leucotis) Ten healthy birds from white cheeked bulbul used,five birds extract the part of trachea and incision it after anaesthetized , then insert the cannulathrough trachea and injected the cold cure mixture for corrosion cast making. and macerated by 2%(KOH) for 4 days. and . Five birds used for imaging examination, barium sulphate suspension wereinjected via the trachea using plastic syringe, for radiographic procedure. The number of the airsacs in bulbul (nine) air sacs, cervical ,cranial and caudal thoracic and abdominal air sac , single airinterclavicular.The shaped and locations of these sacs described by using the cold cure corrosion cast andRadiological examinations.
Mostrar más [+] Menos [-]OCCUREENCE, FOOD NATURE, AGE AND GROWTH OF ORBICULATE BATFISH Platax orbicularis (EPHIPPIDAE) IN IRAQI TERRITORIAL MARINE WATERS Texto completo
2016
Amjed K. Resen
A total of 313 Orbiculate batfishes Platax orbicularis were studied from the Iraqi marine watersat the shallow tip of the Arabian Gulf during the period from March to September 2015. Thisspecies is an omnivore (planktivorous) which migrate between surface and bottom for feedingactivity. Length –Weight relationship and growth rate were calculated for the 222 specimens.The highest growth rate calculated was 5.16 which occurred at length group 23-27cmequivalent to age group I+. The Iraqi marine waters were indicated as feeding ground but notspawning ground.
Mostrar más [+] Menos [-]PROTECTIVE EFFECT OF AQUEOUS EXTRACT OF ZIZYPHUSSPINA CHRISTI FRUIT AGONIST PARACETAMOL INDUCE HEPATOAND HAEMATOTOXICITY IN ADULT MALE RABBITS Texto completo
2016
Qayssar A. Obaid
Zizyphusspina-christi has a common name in Iraq "Nabka",it has been used intraditional medicine.The present study was designed to elucidate the protective effect ofaqueous extract of ziziphusspina Christi on hepato and haematotxicity induced byparacetamol in adult male rabbits. Eighteen adult male rabbits were divided randomly into 3groups of 6 rabbits each and were treated for four weeks as follows:control group, second(T1) groupreceived orally (300 mg/kg B.W.) of paracetmoldailyand third (T2) Groupreceivedorally (200 mg/kg B.W.) of crude extraction of zizyphyusspinadaily with (300 mg /kg B.W.)of paracetmoldaily. The results indicated that paracetamol administration induced hepato andhaematotoxcity manifested by significant(P<0.05) elevation in liver enzymes (AST and ALT)and reduction in total protein and albumin concentration with significant (P<0.05)decrease inRBC, Hb, PCV, MCH, MCHC and Neutrophiles whereas it increase in WBC, MCV,Lymphocyte and monocytes as a compared with control group.On the other hand, animalstreatment with aqueous extract of zizyphusspinachristiin group T2 showed a significantameliorated in biochemical andhaematologicalparameters change induce by paracetamol.Itcould be concluded that oral administration of Zizyphusextract have hepatoprotectiveand antianemicrole agonist hepato and haematotoxicity induce by paracetamol.
Mostrar más [+] Menos [-]THE NUTRITIONAL VALUE AND ANTIOXIDANT ACTIVITY OF BAY LEAVES (laurusn obilis L.) Texto completo
2016
Alaa G. AL-Hashimi | Sawsan A. Mahmood
The present study is to identify the chemical composition of bay leaves(Laurusnobilis L.) protein, oil, ash and carbohydrate which were (7.62, 8.5, 3.63, 50.83)%respectively.This paper reports the concentration of many minerals calcium,Phosphorus,Potassium,Iron,Copper Magnesium, Manganese, and Zink. Calcium andMagnesium have the highest concentration(377,550 mg /g) respectively, Vitaminsconcentration also determined riboflavin, ascorbic acid which were (45.33, 2, 0.90 mg/g)respectively. Bay oil.Was extracted from bay leaves(L.nobilis)with petroleum ether usingSoxhlet apparatus. Bay oil showed high value in unsaturated fatty acids 55% includeoleic,linoleic,linolenic, and saturated fatty acids 45% includelaureic, merestic, palmetic,stearic. The present study describe the reducing power and antioxidant activity foralcoholic extracts of bay leaves.rates of antioxidant activity and reducing power increasesas the concentrate of bay leaves extract increasedwhich were (173.81%) and (75.61%)respectively at 5% concentration.
Mostrar más [+] Menos [-]SEROLOGICAL AND MOLECULAR DETECTION OF TOXOPLASMA GONDII IN MEAT AND MINCED MEAT IN BASRA CITY Texto completo
2016
Battol Ali Majeed | Wamedh Hashim Abbas
The present study was conducted during the period between September 2017and March 2018 to detect Toxoplasma gondii in meat. A total number of 387 blood,meat and minced meat were collected from cattle, sheep, goat and buffalo carcasses atslaughter house and butcher markets in Basra city. Molecular and serological methodswere applied to detect the infection of T. gondii by using PCR and ELISA tests for allthese samples. The results of ELISA showed that 25.32% of samples were positive,while 15.762% were positive for PCR test. Sheep showed the highest positive resultsfor ELISA and PCR tests (33.036%, 22.321% respectively) flowed by goat (29.333%,18.667%), cattle (25.439%,14.912%) and buffalo (11.628%, 4.651%) respectively.Positive ELISA and PCR results for minced meat (37.113%, 25.773% respectively)were higher than meat from both butcher markets (27.778%, 18.519%) andslaughterhouse (17.582%, 9.89%) respectively, while the results of ELISA and PCRconducted for blood analysis were 17.582% and 6.593% respectively. Highertoxoplasmosis ratio (32.195%, 21.951%) for ELISA and PCR respectively forsamples collected from butcher markets in the present study.
Mostrar más [+] Menos [-]ACTIVE SURVEY STUDY OF HAEMONCHOSIS IN SLAUGHTERED SHEEPS AND GOATS AT BASRAH ABATTOIR, BASRAH PROVINCE, IRAQ Texto completo
2016
Huda Abdul hussein Abbas | Suzan A. Al-Azizz
In the current study a total of (705) slaughtered sheeps and goats wereexamined, divided into (556) sheep and (149) goat which were taken from Basrahslaughter house during the period between June 2016 to January 2017.The total number of infected animals with Haemonchosis were (90) which isdivided into (78) sheep and (12) goats while the total number of isolated wormsfrom the abomasum was (2439) for both sheep and goats with total percentage ofinfection12.76 % which was divided in to (14.02% and 8.05 % ) in sheep andgoats respectively, while the intensity of infection was about (27.1)which dividedinto(27.12and 26.91) in sheep and goats respectively.
Mostrar más [+] Menos [-]BIOCHEMICAL AND HISTOLOGICAL CHANGES IN LIVER AND KIDNEY OF CYPRINUS CARPIO L. TREATED WITH ZINC OXIDE(NANO & BULK) Texto completo
2016
Al-Taee & Alhamdani
Comparison of four methods for generating decellularized equine synovial extracellular matrix Texto completo
2016
Reisbig, Nathalie A. | Hussein, Hayam A. | Pinnell, Erin | Bertone, Alicia L.
OBJECTIVE To evaluate 4 methods for generating decellularized equine synovial extracellular matrix. SAMPLE Villous synovium harvested from the femoropatellar and medial femorotibial joints of 4 healthy adult horses < 7 years of age. Synovial samples were frozen (−80°C) until used. PROCEDURES Synovial samples were thawed and left untreated (control) or decellularized with 1 of 4 methods (15 samples/horse/method): incubation in 0.1% peracetic acid (PAA), incubation in 0.1% PAA twice, incubation in 1% Triton X-100 followed by incubation in DNase, and incubation in 2M NaCl followed by incubation in DNase. Control and decellularized samples were examined for residual cells, villous integrity, and collagen structure and integrity by means of histologic examination and scanning electron microscopy; cell viability was evaluated by means of culture and exclusion staining. Decellularization efficiency was assessed by testing for DNA content and DNA fragment size. RESULTS Incubation in PAA once preserved the synovial villous architecture, but resulted in high DNA content and retention of large (> 25,000 base pair) DNA fragments. Incubation in Triton and incubation in NaCl resulted in low DNA content and short (< 200 base pair) DNA fragments, but destroyed the synovial villous architecture. Incubation in PAA twice resulted in low DNA content and short DNA fragments while retaining the synovial villous architecture. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that of the methods evaluated, incubation in 0.1% PAA twice was the best method for generating decellularized equine synovial extracellular matrix.
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