Poultry has been identified as a reservoir of foodborne enteric pathogens and antimicrobial resistant bacteria. The objective of this study was to describe and compare antimicrobial resistant isolates from an Ontario broiler chicken farm-level baseline project (2003 to 2004) to the Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS) Ontario abattoir and retail surveillance data from 2003, and to the most recent (2015) CIPARS Ontario chicken surveillance data in order to assess the impact of an industry-wide policy change in antimicrobial use. Ceftiofur resistance (TIO-R) prevalence in Salmonella decreased by 7% on farm between 2003 and 2004 and 2015. During the same timeframe, TIO-R E. coli prevalence decreased significantly by 16%, 11%, and 8% in farm, abattoir, and retail samples, respectively. Gentamicin resistant (GEN-R) E. coli, however, increased by 10% in farm and 15% in retail-derived isolates, and trimethoprim-sulfamethoxazole resistant (TMSm-R) E. coli increased significantly by 20%, 18%, and 5% in farm, abattoir, and retail isolates, respectively. Similarly, ciprofloxacin-resistant (CIP-R) Campylobacter spp. significantly increased in retail isolates by 11% and increased in farm (33%) and abattoir isolates (7%). The decrease in TIO-R Salmonella/E. coli in recent years is consistent with the timing of an industry-led intervention eliminating the preventive use of ceftiofur, a third generation cephalosporin and class of antimicrobials deemed critically important to human medicine. The rise in GEN-R and TMSm-R prevalence is indicative of recent shifts in antimicrobial use. Our study highlights the importance of integrated surveillance in detecting emerging trends and determining the efficacy of interventions to improve food safety.

OBJECTIVE To evaluate acute changes of the liver by use of shear wave elastography (SWE) and CT perfusion after radiofrequency ablation (RFA). ANIMALS 7 healthy Beagles. PROCEDURES RFA was performed on the liver (day 0). Stiffness of the ablation lesion, transitional zone, and normal parenchyma were evaluated by use of SWE, and blood flow, blood volume, and arterial liver perfusion of those regions were evaluated by use of CT perfusion on days 0 and 4. All RFA lesions were histologically examined on day 4. RESULTS Examination of the SWE color-coded map distinctly revealed stiffness of the liver tissue, which increased from the normal parenchyma to the transitional zone and then to the ablation zone. For CT perfusion, blood flow, blood volume, and arterial liver perfusion decreased from the transitional zone to the normal parenchyma and then to the ablation zone. Tissue stiffness and CT perfusion variables did not differ significantly between days 0 and 4. Histologic examination revealed central diffuse necrosis and peripheral hyperemia with infiltration of lymphoid cells and macrophages. CONCLUSIONS AND CLINICAL RELEVANCE Coagulation necrosis induced a loss of blood perfusion and caused tissue hardening (stiffness) in the ablation zone. Hyperemic and inflammatory changes of the transitional zone resulted in increased blood perfusion. Acute changes in stiffness and perfusion of liver tissue after RFA could be determined by use of SWE and CT perfusion. These results can be used to predict the clinical efficacy of RFA and to support further studies, including those involving hepatic neoplasia.

OBJECTIVE To determine the influence of various neck positions on cervical vertebral radiographic measurements in horses. ANIMALS 18 client-owned horses examined for lameness but free of cervical disease. PROCEDURES Laterolateral radiographs of the cervical vertebrae from C1 through T1 were acquired for each horse in 3 neck positions (low, neutral, and high). Minimum sagittal diameter, intravertebral sagittal diameter (intra-VSD) ratio, inter-VSD ratio, length of the articular processes joint ratio, vertebral alignment angle, and vertebral fossa angle were measured at each segment in each neck position. Values for the high and low positions were compared with those for the neutral position. RESULTS No significant differences from neutral position values were identified for minimum sagittal diameter, intra-VSD ratio, and vertebral fossa angle as measured in low and high neck positions. Compared with results in the neutral position, the high position resulted in a greater vertebral alignment angle at C3–4 and inter-VSD ratio at C4–5 and a lower length of the articular processes joint ratio at C2–3, C3–4, and C4–5; the low position resulted in a lower vertebral alignment angle at C4–5. However, all observed differences were small. CONCLUSIONS AND CLINICAL RELEVANCE Neck position influenced some radiographic measurements of the cervical vertebrae in horses free of cervical disease. However, because several of these measurements were not or were only minimally affected by neck position, some latitude in neck position may be possible without concern about substantially affecting radiographic measurements in this region.

OBJECTIVE To measure cerebral blood flow (CBF) and cerebral blood volume (CBV) by means of perfusion CT in clinically normal Holstein calves. ANIMALS 9 Holstein calves. PROCEDURES Each of the 9 calves (mean age, 20.2 days) was anesthetized and received an injection of iodinated contrast medium into the right jugular vein at a rate of 4.0 mL/s. Dynamic CT scanning of the head at a level that included the mandibular condyle was initiated at the time of the contrast medium injection and continued for 100 seconds. A deconvolution method was used as an analytic algorithm. RESULTS Among the 9 calves, the mean ± SD CBF in the cerebral cortex, white matter, and thalamus was 44.3 ± 10.3 mL/100 g/min, 36.1 ± 7.5 mL/100 g/min, and 40.3 ± 7.5 mL/100 g/min, respectively. The CBF in white matter was significantly lower than that in the cerebral cortex or thalamus. The mean CBV in the cerebral cortex, white matter, and thalamus was 6.8 ± 1.0 mL/100 g, 5.2 ± 1.0 mL/100 g, and 5.7 ± 0.7 mL/100 g, respectively. The CBV in the cerebral cortex was significantly higher than that in the white matter or thalamus. CONCLUSIONS AND CLINICAL RELEVANCE Measurement of CBF and CBV in clinically normal calves by means of perfusion CT was feasible. The data obtained may be useful as baseline values for use in future research or for comparison with findings from calves with CNS diseases. Investigations to determine the lower limit of blood flow at which brain function can still be restored are warranted.

OBJECTIVE To determine effects of restriction feeding of a moderate-protein, high-fiber diet on loss of body weight (BW), voluntary physical activity, body composition, and fecal microbiota of overweight cats. ANIMALS 8 neutered male adult cats. PROCEDURES After BW maintenance for 4 weeks (week 0 = last week of baseline period), cats were fed to lose approximately 1.5% of BW/wk for 18 weeks. Food intake (daily), BW (twice per week), body condition score (weekly), body composition (every 4 weeks), serum biochemical analysis (weeks 0, 1, 2, 4, 8, 12, and 16), physical activity (every 6 weeks), and fecal microbiota (weeks 0, 1, 2, 4, 8, 12, and 16) were assessed. RESULTS BW, body condition score, serum triglyceride concentration, and body fat mass and percentage decreased significantly over time. Lean mass decreased significantly at weeks 12 and 16. Energy required to maintain BW was 14% less than National Research Council estimates for overweight cats and 16% more than resting energy requirement estimates. Energy required for weight loss was 11% more, 6% less, and 16% less than American Animal Hospital Association recommendations for weight loss (80% of resting energy requirement) at weeks 1 through 4, 5 through 8, and 9 through 18, respectively. Relative abundance of Actinobacteria increased and Bacteroidetes decreased with weight loss. CONCLUSIONS AND CLINICAL RELEVANCE Restricted feeding of a moderate-protein, high-fiber diet appeared to be a safe and effective means for weight loss in cats. Energy requirements for neutered cats may be overestimated and should be reconsidered.

Bats have been known as natural reservoirs for potential emerging infectious viruses, such as Lyssaviruses, Coronaviruses, Ebola viruses, Nipah virus, and many others. Because of their abudance in population, wide distribution and mobility, bats have a greater risk as source for zoonotic transmission than other animals. Despite the facts of their role as reservoirs for many pathogens, not until an epidemic of Severe Acute Respiratory Coronavirus (SARS-CoV) in 2003 and Middle-East Respiratory Syndrome Coronavirus (MERS-CoV) in 2012, that people pay much attention about coronavirus in bats. SARS-like virus also found in bats with a higher prevalence rate. This study aims to detect the coronavirus of bats in Gorontalo province Indonesia, characterization at the molecular level of the coronavirus genome and determining the level of kinship (through trees filogenetic). This study was conducted as part of bigger PREDICT Indonesia project, in particular to examine coronavirus in bats from Gorontalo province, Indonesia. As many as 95 rectal swab samples collected from flying foxes (Pteropus alecto) were analyzed in the laboratory using Consensus Polymerase Chain Reaction (PCR) technique to amplify the target sequence from RNA-dependent RNA Polymerase (RdRp) gene with 434 basepair product, resulted 24 samples determined as presumptive positive. Eight out of 24 presumptive positive samples by PCR were analyzed further by nucleotide sequencing and confirmed coronavirus positive. Phylogenetic tree analyses to the eight coronavirus confirmed-sequences were constructed with MEGA-6.0 . The conclusion was 24 out of 95 samples suggested as presumptive positive to Bat CoV. Eight out of 24 samples were analyzed further by nucleotide sequencing and have similarities in the kinship. Three samples had the 98% nucleotide identity to BatCoV from Indonesia and five samples were 85-88% nucleotide identity to BatCoV from Thailand.

This report describes a case of mammary gland tumor in a 1-year-old female cat using a mastectomy approach. The tumor was located on dexter side of mammae. Blood analysis showed good condition of the patient and ready for surgery. Mastectomy was conducted to remove the mass, and the tumor size was measured 7x5x4 cm with solid appearance. The cat was given antibiotics to prevent infection, and the wound dried in five days.

Elephant Training Center (ETC) Way Kambas National Park (WKNP) was built to support human-elephantmitigation conflict. The small population of captive sumatran elephant in ETC WKNP need a comprehensivestrategy in order to maintain the genetic variation of each individual and avoid inbreeding drive. Currently, geneticstudies have opened new field studies in ecology, included conservation ecology. Patterns in variation of populationhas been investigated by molecular method supporting species conservation effort. The captive sumatran elephantsID Card is a necessary in database building, which included morphology, health status, and genetic profile. Geneticprofile in each ID Card was filled by cytogenetic and molecular profile for RADP result, that initiated with DNAisolation. The DNA sources collected by blood sampling protocol described by Asiyah et al. (2016) from captivesumatran elephant in ETC, WKNP, and be carried to laboratory in cold condition. The DNA sources stored at 4oCand isolated following commercial protocol. The result of DNA isolation stored at -20oC until amplificationanalysis. DNA isolation was successfully done, for further individual genetic ID building.

The increase in beef production is constrained by the slow growth of beef cattle population which is caused by the cattle breeding business that is considered less commercially profitable. The supplying of beef needs in a critical and effective manner is always increasing each year, so the price of beef is fluctuate. The various priority concept of the government's main program for the development of beef cattle is always done in every region in Indonesia. But the production of beef is still less and the government have to import the beef to fulfill the beef production. Therefore, this issues need necessary operational steps to provide more open space for some factors and leverage points in the supplying of beef needs. The purpose of this review is to examine the efforts to supply beef needs for the future in Indonesia critically. The high price of beef is not a mistake of the government. Basically, the government has tried to make the beef cattle population increased, to fulfill the consumer needs and the prices of beef is affordable for the customer. To cope with the higher beef prices, the government is make a policy of developing small, medium-sized, and big beef cattle breeding industries through seed cattle spreads on plantations of oil palm, rubber and cultivable fields for the development of beef cattle population. It is expected that in the coming year, beef cattle business can be oriented to agribusiness bussiness so that farmer's welfare will increase and can support Indonesia as the world food granary.

Medical records from 860 pigeons and doves of 24 species housed in Bristol Zoo Gardens and Wild Place Project, United Kingdom, from 2000 to 2017 were reviewed to determine the causes of morbidity in captive columbiformes. Accounted 205 cases (51.25%), infectious disease was found as the primary cause of morbidity, followed by 133 cases of trauma (33.25%), 51 (8.9%) cases of non-infectious disease, and 8 (2%) cases of husbandry-related problem. The main cause of infectious disease was parasite infection with 35% over 31% of bacterial, 26% of undetermined etiology and 9% of fungal infection. More specific, nematodiasis was the most frequent parasitic disease found, presented in 49 (69%) cases and followed by 10 (14%) trichomoniasis of all parasite infection. Meanwhile, chlamydiosis was a leading cause of bacterial infection with 22 (35%) cases recorded of over 63 bacterial infections. These results highlight the importance of preventative medicine implementation in captivity which aims to detect the disease earlier hence reducing the worsening physical condition and minimising the risk of both zoonotic and non-zoonotic disease transmissions. In addition, these findings contribute to the limited information available regarding the captive columbiformes morbidity