Objectives: The study aimed to account for baseline biometrical and histomorphometric testicu¬lar changes in Black Bengal goats during postnatal development. Materials and Methods: Black Bengal goats, divided into group I of VII; day 0; 1, 2 weeks; 1, 2, 4, and 6 months of age, respectively, were used in this study. Results: The biometrical and histomorphometric values of the testis varied significantly (p < 0.05) from postnatal 1–2 months. From day 0 to 2 months, seminiferous tubules, called sex cords, contained simply peripherally placed Sertoli cells and centrally placed gonocytes. Gonocytes, posi¬tioned in the center, moved centrifugally in the direction of the basement membrane of sex cords with the advancement of age, transformed into prespermatogonia, and were distributed among the Sertoli cells at the edge of sex cords that make up the basal cell layer in nearly all of the sem¬iniferous tubules by 2 months after birth. Initiation of spermatogenesis, i.e., stratification and lumination of seminiferous epithelium, took place in the 4th months. At 6 months, all types of spermatogenic cells had been identified. The onset of puberty, i.e., the establishment of sper¬matogenesis, was noticed to have been established at 6 months of postnatal age in Black Bengal goats, as shown by the spermatozoa that were adhered to the ad luminal border of the Sertoli cells and also in the tubular lumen. Conclusion: This research is the first to document the varying biometrical and histomorphometric measurements of the testis in Black Bengal goats from birth to puberty. [J Adv Vet Anim Res 2023; 10(2.000): 237-243]

Objectives: The study aimed to account for baseline biometrical and histomorphometric testicular changes in Black Bengal goats during postnatal development. Materials and Methods: Black Bengal goats, divided into group I of VII; day 0; 1, 2 weeks; 1, 2, 4, and 6 months of age, respectively, were used in this study. Results: The biometrical and histomorphometric values of the testis varied significantly (p < 0.05) from postnatal 1–2 months. From day 0 to 2 months, seminiferous tubules, called sex cords, contained simply peripherally placed Sertoli cells and centrally placed gonocytes. Gonocytes, positioned in the center, moved centrifugally in the direction of the basement membrane of sex cords with the advancement of age, transformed into prespermatogonia, and were distributed among the Sertoli cells at the edge of sex cords that make up the basal cell layer in nearly all of the seminiferous tubules by 2 months after birth. Initiation of spermatogenesis, i.e., stratification and lumination of seminiferous epithelium, took place in the 4th months. At 6 months, all types of spermatogenic cells had been identified. The onset of puberty, i.e., the establishment of spermatogenesis, was noticed to have been established at 6 months of postnatal age in Black Bengal goats, as shown by the spermatozoa that were adhered to the ad luminal border of the Sertoli cells and also in the tubular lumen. Conclusion: This research is the first to document the varying biometrical and histomorphometric measurements of the testis in Black Bengal goats from birth to puberty. J. Adv. Vet. Anim. Res., 10(2): 237–243, June 2023 http://doi.org/10.5455/javar.2023.j674

Objective: This study was conducted to establish a rat model of acute exacerbation of idiopathic pul¬monary fibrosis (AE-IPF) using the combination of bleomycin (BLM) and lipopolysaccharides (LPS). Materials and Method: Twenty-four male Sprague Dawley rats were allocated into two equal groups: the sham or the bleomycin and lipopolysaccharides-induced AE-IPF group (BLM-LPS). On Day 7, BLM intratracheally and LPS intraperitoneally were both used to administer AE-IPF. The BLM-LPS group and its respective sham group were terminated on Days 8, 14, or 21. Samples of bronchoalveolar lavage fluid (BALF) and lungs were taken and investigated for cell count and histopathology. Results: On Day 8, histological analysis revealed inflammatory cell infiltration with edema and hyaline membrane, and the BALF differential cell count revealed high neutrophil counts. By having a higher collagen density area and Ashcroft modified score than the sham group on Day 14, the BLM-LPS group displayed significantly lower oxygen saturation, alveolar air area, and a fibrotic appearance. However, there was a spontaneous resolution in inflammation and fibrotic appearance on Day 21 after the BLM administration. Conclusions: By combining BLM and LPS, it was possible to create a successful rat model of AE-IPF. The present model showed the peak exacerbation on Day 8 and the fibrotic peak on Day 14, which gradually improved. The optimal time for the new AE-IPF therapeutic intervention was determined to be between Days 8 and 14. [J Adv Vet Anim Res 2023; 10(2.000): 196-204]

Objective: This study was conducted to establish a rat model of acute exacerbation of idiopathic pulmonary fibrosis (AE-IPF) using the combination of bleomycin (BLM) and lipopolysaccharides (LPS). Materials and Method: Twenty-four male Sprague Dawley rats were allocated into two equal groups: the sham or the bleomycin and lipopolysaccharides-induced AE-IPF group (BLM-LPS). On Day 7, BLM intratracheally and LPS intraperitoneally were both used to administer AE-IPF. The BLM-LPS group and its respective sham group were terminated on Days 8, 14, or 21. Samples of bronchoalveolar lavage fluid (BALF) and lungs were taken and investigated for cell count and histopathology. Results: On Day 8, histological analysis revealed inflammatory cell infiltration with edema and hyaline membrane, and the BALF differential cell count revealed high neutrophil counts. By having a higher collagen density area and Ashcroft modified score than the sham group on Day 14, the BLM-LPS group displayed significantly lower oxygen saturation, alveolar air area, and a fibrotic appearance. However, there was a spontaneous resolution in inflammation and fibrotic appearance on Day 21 after the BLM administration. Conclusions: By combining BLM and LPS, it was possible to create a successful rat model of AE-IPF. The present model showed the peak exacerbation on Day 8 and the fibrotic peak on Day 14, which gradually improved. The optimal time for the new AE-IPF therapeutic intervention was determined to be between Days 8 and 14.   J. Adv. Vet. Anim. Res., 10(2): 196–204, June 2023 http://doi.org/10.5455/javar.2023.j669

Objective: The study aimed to investigate the hepatoprotective effects of Gastrodia elata rhi¬zome (GR) on thioacetamide (TAA)-induced liver injury in dogs. We evaluated serum biochemical and hematological parameters, with emphasis on alanine transaminase (ALT), alanine phosphates (ALP), and nitric oxide (NO) levels, in dogs with TAA-induced liver injury. Materials and Methods: The animals were divided into a control group (Con), TAA group, Silymarin group (Sil, 50 mg/kg), Gastrodia rhizome low dose (GRL) (low) + TAA, GRH (high) + TAA, and GR high-dose group (GRH) control group. GRL and GRH were given daily at 50 and 100 mg/kg, respectively. TAA was given on days 1, 4, and 7 at a dose of 300 mg/kg. Results: GR significantly reduced liver injury in treated animals, as indicated by lowered levels of ALT (about 32% at day 21 in both GRL + TAA and GRH + TAA groups), ALP (about 17% and 21% at day 21 in both GRL + TAA, GRH + TAA groups, respectively), and NO (about 36% at day 21 in both GRL + TAA, GRH + TAA groups) compared to the TAA control group. Hematological parameters showed mild changes during the experiment. High-performance liquid chromatography analysis revealed gastrodin, a major component of the GR extract, constitutes 2.6% of the extract. Conclusion: The GR demonstrated significant hepatoprotective effects against TAA-induced liver injury in dogs. The study provides evidence for the potential therapeutic use of GR in the man¬agement of liver diseases. [J Adv Vet Anim Res 2023; 10(2.000): 144-150]

Objective: The study aimed to investigate the hepatoprotective effects of Gastrodia elata rhizome (GR) on thioacetamide (TAA)-induced liver injury in dogs. We evaluated serum biochemical and hematological parameters, with emphasis on alanine transaminase (ALT), alanine phosphates (ALP), and nitric oxide (NO) levels, in dogs with TAA-induced liver injury. Materials and Methods: The animals were divided into a control group (Con), TAA group, Silymarin group (Sil, 50 mg/kg), Gastrodia rhizome low dose (GRL) (low) + TAA, GRH (high) + TAA, and GR high-dose group (GRH) control group. GRL and GRH were given daily at 50 and 100 mg/kg, respectively. TAA was given on days 1, 4, and 7 at a dose of 300 mg/kg. Results: GR significantly reduced liver injury in treated animals, as indicated by lowered levels of ALT (about 32% at day 21 in both GRL + TAA and GRH + TAA groups), ALP (about 17% and 21% at day 21 in both GRL + TAA, GRH + TAA groups, respectively), and NO (about 36% at day 21 in both GRL + TAA, GRH + TAA groups) compared to the TAA control group. Hematological parameters showed mild changes during the experiment. High-performance liquid chromatography analysis revealed gastrodin, a major component of the GR extract, constitutes 2.6% of the extract. Conclusion: The GR demonstrated significant hepatoprotective effects against TAA-induced liver injury in dogs. The study provides evidence for the potential therapeutic use of GR in the management of liver diseases.    J. Adv. Vet. Anim. Res., 10(2): 144–150, June 2023 http://doi.org/10.5455/javar.2023.j663

Objectives: Ducks suffer a huge economic loss as a result of infections with Pasteurella multocida and Riemerella anatipestifer, which cause high morbidity and mortality. Because these patho¬gens induce similar clinical symptoms when coinfections occur, it is very difficult to differentiate between them based just on clinical signs. Hence, these major pathogens must be quickly and accurately detected. Materials and Methods: A total of 104 birds ranging from 2 days to 4 weeks old were collected from Egyptian farms, and the outcomes were compared statistically. Conventional cultural iden¬tification procedures and a direct multiplex polymerase chain reaction assay were utilized to recognize both pathogens in a single tube reaction simultaneously. Then, the obtained isolates were characterized phenotypically and genotypically. Results: Clinical signs appear at 2–4 weeks of age with respiratory distress (dyspnea), white fluid feces, and stunting. The scrutinized data demonstrated a significantly higher detection rate by PCR directly compared to classical culture procedures. Pasteurella multocida was detected only by PCR. The disc diffusion technique against ten antibiotics showed absolute susceptibilities to amik¬acin, doxycycline, and florfenicol. High levels of beta-lactam resistance were observed. Riemerella anatipestifer isolates were screened for pathogenicity and plasmid-borne blaTEM genes. All six isolates harbored five virulence genes: aspC, RA46, m28, pstS, and Nlp/P60. Moreover, blaTEM was identified into four isolates and deposited to GenBank with accession numbers OP347083, OP347084, OP347085, and OP347086. Conclusion: These results suggest advanced PCR assays can be applied to the field for rapid and valuable diagnosis of two significant pathogens and focus on the worth of ducks in the propaga¬tion of transferable antibiotic resistance genes into the environment. [J Adv Vet Anim Res 2023; 10(2.000): 211-221]

Objectives: Ducks suffer a huge economic loss as a result of infections with Pasteurella multocida and Riemerella anatipestifer, which cause high morbidity and mortality. Because these pathogens induce similar clinical symptoms when coinfections occur, it is very difficult to differentiate between them based just on clinical signs. Hence, these major pathogens must be quickly and accurately detected. Materials and Methods: A total of 104 birds ranging from 2 days to 4 weeks old were collected from Egyptian farms, and the outcomes were compared statistically. Conventional cultural identification procedures and a direct multiplex polymerase chain reaction assay were utilized to recognize both pathogens in a single tube reaction simultaneously. Then, the obtained isolates were characterized phenotypically and genotypically. Results: Clinical signs appear at 2–4 weeks of age with respiratory distress (dyspnea), white fluid feces, and stunting. The scrutinized data demonstrated a significantly higher detection rate by PCR directly compared to classical culture procedures. Pasteurella multocida was detected only by PCR. The disc diffusion technique against ten antibiotics showed absolute susceptibilities to amikacin, doxycycline, and florfenicol. High levels of beta-lactam resistance were observed. Riemerella anatipestifer isolates were screened for pathogenicity and plasmid-borne blaTEM genes. All six isolates harbored five virulence genes: aspC, RA46, m28, pstS, and Nlp/P60. Moreover, blaTEM was identified into four isolates and deposited to GenBank with accession numbers OP347083, OP347084, OP347085, and OP347086. Conclusion: These results suggest advanced PCR assays can be applied to the field for rapid and valuable diagnosis of two significant pathogens and focus on the worth of ducks in the propagation of transferable antibiotic resistance genes into the environment.  J. Adv. Vet. Anim. Res., 10(2): 211–221, June 2023 http://doi.org/10.5455/javar.2023.j671

Objectives: This study aimed to investigate the prevalence and pattern of congenital flexural deformity (knuckling), to identify the association between trace elements and vitamins with the deformity, and to apply different surgical techniques for correcting this congenital malformation in newborn bovine calves. Materials and Methods: The study was implemented on 17 newborn calves with carpal (knee) and fetlock (foot) knuckling presented to the Veterinary Teaching Hospital of Bangladesh Agricultural University, Mymensingh, from January to December 2020. The serum biochemical alterations and clinical outcomes were assessed on days 0 and 21 following surgery. Two surgical methods: ten¬don transection and tendon elongation by Z-tenotomy, were performed for surgical restoration. Results: We found that knuckling comprised 12% of the total congenitally malformed calves. The male calves had a higher prevalence (52%, n = 9) and the same in the winter season (65%, n = 11). The majority of the knuckling was bilateral types (88%, n = 15), involving the carpal joint (82%, n = 14) and moderately angulated (59%, n = 10). The serum level of magnesium, iron, vitamin D, and zinc were significantly (p < 0.05) changed from the pre-surgical stage to the stage of non-lameness after surgery. The disorder was surgically treated by tendon transection or tendon elongation procedure and had a good prognosis. Conclusion: The current study concluded that the development of knuckling in calves might be related to a deficiency/excess of specific minerals and vitamins and that it can be effectively cor¬rected by surgical intervention; however, early diagnosis and the use of proper surgical techniques are crucial for improving the prognosis. [J Adv Vet Anim Res 2023; 10(1.000): 88-95]

Objectives: This study aimed to investigate the prevalence and pattern of congenital flexural deformity (knuckling), to identify the association between trace elements and vitamins with the deformity, and to apply different surgical techniques for correcting this congenital malformation in newborn bovine calves. Materials and Methods: The study was implemented on 17 newborn calves with carpal (knee) and fetlock (foot) knuckling presented to the Veterinary Teaching Hospital of Bangladesh Agricultural University, Mymensingh, from January to December 2020. The serum biochemical alterations and clinical outcomes were assessed on days 0 and 21 following surgery. Two surgical methods: tendon transection and tendon elongation by Z-tenotomy, were performed for surgical restoration. Results: We found that knuckling comprised 12% of the total congenitally malformed calves. The male calves had a higher prevalence (52%, n = 9) and the same in the winter season (65%, n = 11). The majority of the knuckling was bilateral types (88%, n = 15), involving the carpal joint (82%, n = 14) and moderately angulated (59%, n = 10). The serum level of magnesium, iron, vitamin D, and zinc were significantly (p < 0.05) changed from the pre-surgical stage to the stage of non-lameness after surgery. The disorder was surgically treated by tendon transection or tendon elongation procedure and had a good prognosis. Conclusion: The current study concluded that the development of knuckling in calves might be related to a deficiency/excess of specific minerals and vitamins and that it can be effectively corrected by surgical intervention; however, early diagnosis and the use of proper surgical techniques are crucial for improving the prognosis. J. Adv. Vet. Anim. Res., 10(1): 88–95, March 2023 http://doi.org/10.5455/javar.2023.j656

Objective: The research was conducted to calculate feed, calcium (Ca) intake, Ca requirements, and egg production for Arabic hens during the early egg-laying period. Materials and Methods: A total of 135 30-week-old Silver female Arabic pullets were randomly allocated to one of three treatments with five replicate cages with a semi-scavenging system of nine pullets per cage in a completely randomized design and allowed to choose Ca from limestone and oyster shells. As a control (T1), pullets were only given a complete feed with Ca and phosphorus percentages according to Hy-line International in 2018. Other treatment feeds were control feed without limestone fed with a combination of limestone (T2) or oyster shell (T3) separately. Results: The treatments had no effect (p > 0.05) on feed, grit and Ca intake (gm/bird/week), egg weight (gm), egg mass, egg production (%), and feed efficiency, but had an effect (p < 0.05) on Ca concentration (%). Ca concentration was the same at T1 and T3, and both were higher than at T2. Conclusion: The female Arabic chickens could fulfill Ca requirements by selecting from different sources of Ca. Limestone is better than the oyster shell as a source of Ca. The Ca requirement for Arabic hens in the early laying period based on the Ca concentration of the feed intake is sufficient at around 3.64% since producing the same number of egg production and heavier egg weight compared to a higher Ca level. [J Adv Vet Anim Res 2023; 10(1.000): 30-41]

Objective: The research was conducted to calculate feed, calcium (Ca) intake, Ca requirements, and egg production for Arabic hens during the early egg-laying period. Materials and Methods: A total of 135 30-week-old Silver female Arabic pullets were randomly allocated to one of three treatments with five replicate cages with a semi-scavenging system of nine pullets per cage in a completely randomized design and allowed to choose Ca from limestone and oyster shells. As a control (T1), pullets were only given a complete feed with Ca and phosphorus percentages according to Hy-line International in 2018. Other treatment feeds were control feed without limestone fed with a combination of limestone (T2) or oyster shell (T3) separately. Results: The treatments had no effect (p > 0.05) on feed, grit and Ca intake (gm/bird/week), egg weight (gm), egg mass, egg production (%), and feed efficiency, but had an effect (p < 0.05) on Ca concentration (%). Ca concentration was the same at T1 and T3, and both were higher than at T2. Conclusion: The female Arabic chickens could fulfill Ca requirements by selecting from different sources of Ca. Limestone is better than the oyster shell as a source of Ca. The Ca requirement for Arabic hensin the early laying period based on the Ca concentration of the feed intake is sufficient at around 3.64% since producing the same number of egg production and heavier egg weight compared to a higher Ca level. J. Adv. Vet. Anim. Res., 10(1): 30–41, March 2023 http://doi.org/10.5455/javar.2023.j649

Objective: The percentage of infertility cases in this world is about 50%. The seahorse (Hippocampus spp.) is wide to traditional medicine. Several studies stated that seahorse has eth¬nopharmacological features, such as fertility, antioxidants, and antifatigue. The purpose of this study was to determine whether seahorse extract (SE) (Hippocampus comes L.) affects fertility and serum biochemistry in rats induced by depo medroxyprogesterone acetate (DMPA). Materials and Methods: All animals were induced by 1.25 mg/kg BW DMPA. The animals were grouped into five groups: aquadest, CMC 1%, and SE doses of 150, 225, and 300 mg/kg BW. The rats were gavage every morning from week 7 until 18. At the end of our study, the semen from the vas deferens and blood from the heart were analyzed. We analyzed with a one-way analysis of variance and Bonferroni's post hoc tests (α 95%). Results: The concentration of spermatozoa had a significant difference in dose of 150 mg/kg BW compared to other groups (p = 0.04). In contrast, motility (p = 0.012) and viability of spermatozoa (p = 0.007) were highly significant differences (p < 0.05 and p < 0.01) in dose 300 mg/kg BW. The testosterone levels have not significantly (p = 0.162; p > 0.05), but the tendency increased in dose 300 mg/kg BW (11.01%). Nevertheless, serum biochemistry was insignificant (p > 0.05) in all groups. Conclusion: The SE ameliorates fertility and serum biochemistry in DMPA-induced rats. [J Adv Vet Anim Res 2023; 10(1.000): 126-131]

Objective: The percentage of infertility cases in this world is about 50%. Seahorses (Hippocampus spp.) are widely used in traditional medicine. Several studies suggest that seahorses have ethno pharmacological characteristics, such as fertility, antioxidants, and antifatigue. The purpose of this study was to determine whether seahorse extract (SE) (Hippocampus comes L.) has an effect on fertility and serum biochemistry in rats induced by depo medroxyprogesterone acetate (DMPA). Materials and Methods: All animals were induced with 1.25 mg/kg BW of DMPA. Animals were grouped into five groups: namely aquadest, 1% CMC, and SE doses of 150, 225, and 300 mg/kg BW. The rats were gavage every morning from week 7 until 18. At the end of our study, semen from the vas deferens and blood from the heart were analyzed. We analyzed with a one-way analysis of variance and Bonferroni’s post hoc tests (α 95%). Results: The concentration of spermatozoa had a significant difference in dose of 150 mg/kg BW compared to other groups (p = 0.04). In contrast, the motility (p = 0.012) and viability of spermatozoa (p = 0.007) were highly significant differences (p < 0.05 and p < 0.01) at 300 mg/kg BW. Testosterone levels were not significantly (p = 0.162; p > 0.05), but tended to increase at 300 mg/ kg BW (11.01%). Nevertheless, serum biochemistry was insignificant (p > 0.05) in all groups. Conclusion: SE (Hippocampus comes L.) ameliorates fertility and serum biochemistry in rats induced by DMPA. J. Adv. Vet. Anim. Res., 10(1): 126–131, March 2023 http://doi.org/10.5455/javar.2023.j661