Objective: This research aimed to assess the prevalence of caprine pasteurellosis, isolate and identify pasteurellosis (Mannheimia haemolytica and Pasteurella multocida) in pneumonic goats, and discover the main bacterial cause of pneumonia. Materials and Methods: One hundred and five samples (94 nasal swabs and 11 lung tissues) from goats suspected of having pneumonia were taken and transferred aseptically to the laboratory. Following the processing of the collected samples, Pasteurella spp. was isolated with the aid of plate culture methods. Biochemical characteristics were used to identify all bacterial isolates, which were then verified by polymerase chain reaction (PCR). Antimicrobial susceptibility testing was also carried out to evaluate the sensitivity profiles of various antibiotics. The Pasteurella haemolytica serotype-specific antigen (PHSSA) gene was used to identify isolates of M. haemolytica, and the KMT1 gene was used to identify isolates of P. multocida. Results: From the 105 clinically suspicious samples, 51 (48.57%) were identified to be Pasteurella spp. through bacteriological testing and also by PCR targeting the 16S rRNA gene. Of these, 47.87% (45/94) were nasal swabs, and 54.55% (6/11) were lung tissues. Among confirmed samples, 70.59% (36/51) were identified as M. haemolytica, and 29.41% (15/51) were identified as P. multocida. Resistance to tetracycline, streptomycin, oxytetracycline, gentamicin, and ceftriaxone was found in 50%–83% of the isolates. In addition, PCR identified the PHSSA and KMT1 genes from isolates of P. multocida and M. haemolytica, respectively. Conclusion: The present study revealed that M. haemolytica and P. multocida primarily caused pasteurellosis in pneumonic goats in Bangladesh. However, when treating these animals, the proper choice of antimicrobials should be made to control this disease. J. Adv. Vet. Anim. Res., 10(3): 538–544, September 2023 http://doi.org/10.5455/javar.2023.j707

Objective: This research aimed to assess the prevalence of caprine pasteurellosis, isolate and identify pasteurellosis (Mannheimia haemolytica and Pasteurella multocida) in pneumonic goats, and discover the main bacterial cause of pneumonia. Materials and Methods: One hundred and five samples (94 nasal swabs and 11 lung tissues) from goats suspected of having pneumonia were taken and transferred aseptically to the laboratory. Following the processing of the collected samples, Pasteurella spp. was isolated with the aid of plate culture methods. Biochemical characteristics were used to identify all bacterial isolates, which were then verified by polymerase chain reaction (PCR). Antimicrobial susceptibility testing was also carried out to evaluate the sensitivity profiles of various antibiotics. The Pasteurella hae¬molytica serotype-specific antigen (PHSSA) gene was used to identify isolates of M. haemolytica, and the KMT1 gene was used to identify isolates of P. multocida. Results: From the 105 clinically suspicious samples, 51 (48.57%) were identified to be Pasteurella spp. through bacteriological testing and also by PCR targeting the 16S rRNA gene. Of these, 47.87% (45/94) were nasal swabs, and 54.55% (6/11) were lung tissues. Among confirmed sam¬ples, 70.59% (36/51) were identified as M. haemolytica, and 29.41% (15/51) were identified as P. multocida. Resistance to tetracycline, streptomycin, oxytetracycline, gentamicin, and ceftriax¬one was found in 50%–83% of the isolates. In addition, PCR identified the PHSSA and KMT1 genes from isolates of P. multocida and M. haemolytica, respectively. Conclusion: The present study revealed that M. haemolytica and P. multocida primarily caused pasteurellosis in pneumonic goats in Bangladesh. However, when treating these animals, the proper choice of antimicrobials should be made to control this disease.

Objective: The escalating prevalence of canine oral tumors has emerged as a considerable health concern. This study examined the prevalence, types, and distributions of lesions linked to canine oral tumors. Material and Methods: The medical records of 526 dogs diagnosed with oral tumors were analyzed to determine the prevalence, types, and distributions. Tumor stages were classified into four categories using the tumor node metastasis system. Results: Among the 526 dogs, there were 118 cases of benign tumors and 408 cases of malignant tumors. Acanthomatous ameloblastoma was the most common benign tumor (43.22%), while melanoma was the most common malignant tumor (51.23%). The gingiva was the most common site for both benign and malignant lesions, accounting for 89.83% and 63.73% of cases, respectively. Melanoma, squamous cell carcinoma, and fibrosarcoma were primarily located in the gingiva, whereas osteosarcoma was commonly found in the mandible. Most tumors were classified as stage III (ranging from 46.84% to 74.58%). Of the reported cases, 56.08% were males and 43.92% were females, and the most common breed was mixed at 30.41%, followed by Poodle at 14.25% and Shih Tzu at 11.40%. Moreover, patients with malignant oral tumors (11.6 ± 3.1 years) were significantly older than those with benign tumors (8.9 ± 3.4 years, p < 0.0001). Conclusion: Gingiva was the primary site for oral tumors, and mainly classified as stage III. These findings emphasize the increasing occurrence of oral tumors in senior and geriatric dogs and provide insights into the prevalent types and distribution.  J. Adv. Vet. Anim. Res., 10(3): 554–562, September 2023 http://doi.org/10.5455/javar.2023.j709

Objective: The escalating prevalence of canine oral tumors has emerged as a considerable health concern. This study examined the prevalence, types, and distributions of lesions linked to canine oral tumors. Material and Methods: The medical records of 526 dogs diagnosed with oral tumors were analyzed to determine the prevalence, types, and distributions. Tumor stages were classified into four categories using the tumor node metastasis system. Results: Among the 526 dogs, there were 118 cases of benign tumors and 408 cases of malignant tumors. Acanthomatous ameloblastoma was the most common benign tumor (43.22%), while melanoma was the most common malignant tumor (51.23%). The gingiva was the most common site for both benign and malignant lesions, accounting for 89.83% and 63.73% of cases, respectively. Melanoma, squamous cell carcinoma, and fibrosarcoma were primarily located in the gin¬giva, whereas osteosarcoma was commonly found in the mandible. Most tumors were classified as stage III (ranging from 46.84% to 74.58%). Of the reported cases, 56.08% were males and 43.92% were females, and the most common breed was mixed at 30.41%, followed by Poodle at 14.25% and Shih Tzu at 11.40%. Moreover, patients with malignant oral tumors (11.6 ± 3.1 years) were significantly older than those with benign tumors (8.9 ± 3.4 years, p < 0.0001). Conclusion: Gingiva was the primary site for oral tumors, and mainly classified as stage III. These findings emphasize the increasing occurrence of oral tumors in senior and geriatric dogs and provide insights into the prevalent types and distribution.

Objective: The research aimed to isolate, adapt to cell culture, and characterize the lumpy skin disease virus (LSDV) from clinically infected cattle in Bangladesh. Materials and Methods: From September 2019 to June 2020, 37 skin nodules and skin swabs were aseptically collected from afflicted cattle in the outbreak regions of Jhenaidah and Kishoreganj in Bangladesh. The LSDV was isolated from embryonated specific pathogen-free (SPF) chicken eggs along the chorioallantoic membrane (CAM) route and the Vero cell line after several blind passages. The viral attachment protein was targeted for molecular detection using polymerase chain reactions (PCR). For phylogenetic analysis, PCR-positive products were partially sequenced. Results: The virus was evident in the cell line, showed cytopathic effects after the 13 blind passage, and on the CAM of SPF chicken eggs, exhibited thickening of the CAM with pock-like lesions. A total of 12 samples (32.43%) tested positive for LSDV by PCR. Phylogenetic analysis of the present isolates (accession numbers MN792649 and MN792650 ) revealed 100% similarity with strains from India (MN295064), Kenya (AF325528, MN072619, KX683219), Greece (KY829023), Serbia (KY702007), and Kazakhstan (MN642592); moreover, 99.43% to 100% similarity to the sheep pox virus. Conclusion: Partially sequenced LSDV was developed as a vaccine seed and was first isolated in Bangladesh and characterized at the molecular level. J. Adv. Vet. Anim. Res., 10(3):563–569, September 2023 http://doi.org/10.5455/javar.2023.j710

Objective: The research aimed to isolate, adapt to cell culture, and characterize the lumpy skin disease virus (LSDV) from clinically infected cattle in Bangladesh. Materials and Methods: From September 2019 to June 2020, 37 skin nodules and skin swabs were aseptically collected from afflicted cattle in the outbreak regions of Jhenaidah and Kishoreganj in Bangladesh. The LSDV was isolated from embryonated specific pathogen-free (SPF) chicken eggs along the chorioallantoic membrane (CAM) route and the Vero cell line after several blind pas¬sages. The viral attachment protein was targeted for molecular detection using polymerase chain reactions (PCR). For phylogenetic analysis, PCR-positive products were partially sequenced. Results: The virus was evident in the cell line, showed cytopathic effects after the 13 blind pas¬sage, and on the CAM of SPF chicken eggs, exhibited thickening of the CAM with pock-like lesions. A total of 12 samples (32.43%) tested positive for LSDV by PCR. Phylogenetic analysis of the present isolates (accession numbers MN792649 and MN792650) revealed 100% similarity with strains from India (MN295064), Kenya (AF325528, MN072619, KX683219), Greece (KY829023), Serbia (KY702007), and Kazakhstan (MN642592); moreover, 99.43% to 100% similarity to the sheep pox virus. Conclusion: Partially sequenced LSDV was developed as a vaccine seed and was first isolated in Bangladesh and characterized at the molecular level.

Objective: The aim of the study was to use a meta-analysis to identify the correlation between linear body measurements, including body length (BL), wither height (WH), heart girth (HG), and body volume (BV), and body weight in beef cattle by breed, sex, and age as categories. Materials and methods: These results can be used as a method for predicting beef cattle body weight. This study used systematic review and meta-analysis guidelines to create a checklist. The first stage was searching for papers relevant to the study objectives. The second stage was searching using the keywords beef cattle, body weight, body measurement, and correlation. The third stage was reviewing the title and abstract. The fourth stage was abstracting information from selected papers, and the last stage was tabulating data. Results: The results from this study were obtained, and 32 papers were eligible for the meta-analysis stage. The correlation between linear body measurement and body weight of beef cattle showed that HG (r = 0.88) and BV (r = 0.97) were significantly (p < 0.05) different compared to BL (r = 0.74) and WH (r = 0.72). The correlation between HG and body weight, and the categorization of cattle breeds showed significantly (p < 0.05) different results. The correlation between BV and body weight of cattle according to breed categories showed results that were not significantly (p > 0.05) different, while age was significantly (p < 0.05). Conclusion: In conclusion, to predict beef cattle body weight, it is necessary to use HG or BV, with breed, sex, and age of cattle as categories.  J. Adv. Vet. Anim. Res., 10(4): 630–638, December 2023 http://doi.org/10.5455/javar.2023.j718

Objective: The aim of the study was to use a meta-analysis to identify the correlation between linear body measurements, including body length (BL), wither height (WH), heart girth (HG), and body volume (BV), and body weight in beef cattle by breed, sex, and age as categories. Materials and Methods: These results can be used as a method for predicting beef cattle body weight. This study used systematic review and meta-analysis guidelines to create a checklist. The first stage was searching for papers relevant to the study objectives. The second stage was searching using the keywords beef cattle, body weight, body measurement, and correlation. The third stage was reviewing the title and abstract. The fourth stage was abstracting information from selected papers, and the last stage was tabulating data. Results: The results from this study were obtained, and 32 papers were eligible for the meta-analysis stage. The correlation between linear body measurement and body weight of beef cattle showed that HG (r = 0.88) and BV (r = 0.97) were significantly (p < 0.05) different compared to BL (r = 0.74) and WH (r = 0.72). The correlation between HG and body weight, and the categorization of cattle breeds showed significantly (p < 0.05) different results. The correlation between BV and body weight of cattle according to breed categories showed results that were not significantly (p > 0.05) different, while age was significantly (p < 0.05). Conclusion: In conclusion, to predict beef cattle body weight, it is necessary to use HG or BV, with breed, sex, and age of cattle as categories. [J Adv Vet Anim Res 2023; 10(4.000): 630-638]

Objective: This study investigated the effectiveness of different physical forms of feed containing Ipomoea aquatica waste and concentrate feed on the rumen characteristics, chewing activity, and performance of sheep. Materials and Methods: Twenty-four rams (19.87 ± 2.19 kg) were arranged in a completely randomized design. Rams were fed dried I. aquatica waste and concentrate feed provided separately (RCF) (conventional feeding system), and total mixed ration consisted of mash complete feed (MCF), and pelleted complete feed (PCF). The data were analyzed using a one-way analysis of variance. Results: The dry matter intake (DMI), average daily gain, and feed conversion ratio of rams fed different physical forms of feed containing I. aquatica waste in their diet were similar between the treatments, ranging from 4.08% to 4.29% of body weight, 120–180 gm, and 6.32–9.17, respectively. Different physical feeds did not affect microbial synthesis in the rumen. Methane emissions per unit of production were similar between the groups. The PCF sheep ate faster (0.24 min/gm DMI) than the MCF sheep (0.38 min/gm DMI), but similar to the RCF sheep (0.31 min/gm DMI). Conclusion: Dried I. aquatica waste was useful as an alternative lamb feed roughage during the dry season. The pelleted mixed ration was more efficient than mash in increasing DMI. The dried I. aquatica waste was environmentally friendly for mitigating enteric methane emissions by sheep. J. Adv. Vet. Anim. Res., 10(4): 677–684, December 2023 http://doi.org/10.5455/javar.2023.j723

Objective: This study investigated the effectiveness of different physical forms of feed containing Ipomoea aquatica waste and concentrate feed on the rumen characteristics, chewing activity, and performance of sheep. Materials and Methods: Twenty-four rams (19.87 ± 2.19 kg) were arranged in a completely randomized design. Rams were fed dried I. aquatica waste and concentrate feed provided separately (RCF) (conventional feeding system), and total mixed ration consisted of mash complete feed (MCF), and pelleted complete feed (PCF). The data were analyzed using a one-way analysis of variance. Results: The dry matter intake (DMI), average daily gain, and feed conversion ratio of rams fed different physical forms of feed containing I. aquatica waste in their diet were similar between the treatments, ranging from 4.08% to 4.29% of body weight, 120–180 gm, and 6.32–9.17, respectively. Different physical feeds did not affect microbial synthesis in the rumen. Methane emissions per unit of production were similar between the groups. The PCF sheep ate faster (0.24 min/gm DMI) than the MCF sheep (0.38 min/gm DMI), but similar to the RCF sheep (0.31 min/gm DMI). Conclusion: Dried I. aquatica waste was useful as an alternative lamb feed roughage during the dry season. The pelleted mixed ration was more efficient than mash in increasing DMI. The dried I. aquatica waste was environmentally friendly for mitigating enteric methane emissions by sheep. [J Adv Vet Anim Res 2023; 10(4.000): 677-684]

Objective: This study sought to determine the effectiveness of the cryodehydration technique in preserving the morphologic and morphometric attributes of the anatomical specimens of goats. Materials and methods: Different anatomical parts of a goat, i.e., heart, lungs, spleen, liver, kidney, and musculoskeletal specimens, were collected and fixed in 15% formalin. Later on, the fixed specimens were cryodehydrated by fast freezing (burning process) and repeated freezing-thawing sessions, followed by wood glue coating. Finally, the macroscopic (i.e., weight, color, texture, odor, and durability) and microscopic characteristics (by routine hematoxylin and eosin staining) of the cryodehydrated specimens were studied. Results: The resultant specimens produced excellent color and texture and were lightweight (60%–80% weight loss), soft, dry, odorless, durable, and easy to handle. The histoarchitectural details of the heart and skeletal muscle were well preserved, while some distinctive alterations were observed in the parenchymatous organs, i.e., breach in cellular integrity, loss of cell cytoplasm, loss of cytoplasmic and nuclear clarity, increased sinusoidal space, dilatation of the renal tubules, and reduction in glomerular size. Nevertheless, the basic histoarchitecture of each specimen was yet to be distinctly identifiable. Conclusion: The current study findings suggest that the cryodehydration technique can preserve gross anatomical features as well as histoarchitectural details and can be an effective tool for facilitating veterinary education and research. J. Adv. Vet. Anim. Res., 10(4): 720–729, December 2023 http://doi.org/10.5455/javar.2023.j727

Objective: This study sought to determine the effectiveness of the cryodehydration technique in preserving the morphologic and morphometric attributes of the anatomical specimens of goats. Materials and methods: Different anatomical parts of a goat, i.e., heart, lungs, spleen, liver, kidney, and musculoskeletal specimens, were collected and fixed in 15% formalin. Later on, the fixed specimens were cryodehydrated by fast freezing (burning process) and repeated freezing-thawing sessions, followed by wood glue coating. Finally, the macroscopic (i.e., weight, color, texture, odor, and durability) and microscopic characteristics (by routine hematoxylin and eosin staining) of the cryodehydrated specimens were studied. Results: The resultant specimens produced excellent color and texture and were lightweight (60%–80% weight loss), soft, dry, odorless, durable, and easy to handle. The histoarchitectural details of the heart and skeletal muscle were well preserved, while some distinctive alterations were observed in the parenchymatous organs, i.e., breach in cellular integrity, loss of cell cytoplasm, loss of cytoplasmic and nuclear clarity, increased sinusoidal space, dilatation of the renal tubules, and reduction in glomerular size. Nevertheless, the basic histoarchitecture of each specimen was yet to be distinctly identifiable. Conclusion: The current study findings suggest that the cryodehydration technique can preserve gross anatomical features as well as histoarchitectural details and can be an effective tool for facilitating veterinary education and research. [J Adv Vet Anim Res 2023; 10(4.000): 720-729]

Objective: This study aimed to examine the concentration of nonessential amino acids (NEAAs) in ruminant feed in tropical areas, with a focus on forage grasses and legumes in Indonesia. Materials and Methods: A total of 11 grasses (Chrysopogon zizanioides, Brachiaria brizantha, Brachiaria humidicola, Paspalum dilatatum, Paspalum atatum, Chloris gayana, Pennisetum polystachion, Panicum maximum, Cenchrus biflorus, Andropogon canaliculatus, and Digotaria decumbens) and six legumes (Arachis hypogaea, Pueararia Javanica, Centrosema pubescens, Clitoria ternatea, and Arachis pintoi) were analyzed for NEAA content using high-performance liquid chromatography (HPLC). Results: Based on the results of this research, it was found that almost all NEAA content in forage was less than 3% (Serine (Ser), Alanine (Ala), Glycine (Gly), Tyrosine (Tyr), Proline (Pro), Cysteine (Cys), and Asparagine (Asn), except for glutamic (Glu) acid and arginine (Arg) in some legumes. The Glu content in grass ranges from 0.76% to 2.61%, and the Arg content ranges from 0.92% to 2.09%. These two NEAAs were most abundant in grasses and legumes, with concentrations of 5.10% to 6.27% and 3.10% to 5.53%, respectively. Conclusion: Our study concluded that Glu and Arg were the most abundant NEAAs in tropical forages in Indonesia, with legumes having a higher concentration of NEAAs compared to grasses. Among the legumes, A. hypogaea had the highest NEAA content (23.40%), while among the forages, C. zizanioides had the highest NEAA content (12.37%). However, it was observed that neither legumes nor grasses could fulfill the metabolizable TNEAAs requirements for gaining cattle (250 kg of empty body weight gain), unlike commercial concentrates, which were found to meet the requirements, especially for Arg, Glu, and thyronine. The provision of concentrate is necessary to supplement forage to meet the NEAA needs for cattle. J. Adv. Vet. Anim. Res., 10(4): 820–829, December 2023 http://doi.org/10.5455/javar.2023.j739

Objective: This study aimed to examine the concentration of nonessential amino acids (NEAAs) in ruminant feed in tropical areas, with a focus on forage grasses and legumes in Indonesia. Materials and Methods: A total of 11 grasses (Chrysopogon zizanioides, Brachiaria brizantha, Brachiaria humidicola, Paspalum dilatatum, Paspalum atatum, Chloris gayana, Pennisetum polystachion, Panicum maximum, Cenchrus biflorus, Andropogon canaliculatus, and Digotaria decumbens) and six legumes (Arachis hypogaea, Pueararia Javanica, Centrosema pubescens, Clitoria ternatea, and Arachis pintoi) were analyzed for NEAA content using high-performance liquid chromatography (HPLC). Results: Based on the results of this research, it was found that almost all NEAA content in forage was less than 3% (Serine (Ser), Alanine (Ala), Glycine (Gly), Tyrosine (Tyr), Proline (Pro), Cysteine (Cys), and Asparagine (Asn), except for glutamic (Glu) acid and arginine (Arg) in some legumes. The Glu content in grass ranges from 0.76% to 2.61%, and the Arg content ranges from 0.92% to 2.09%. These two NEAAs were most abundant in grasses and legumes, with concentrations of 5.10% to 6.27% and 3.10% to 5.53%, respectively. Conclusion: Our study concluded that Glu and Arg were the most abundant NEAAs in tropical forages in Indonesia, with legumes having a higher concentration of NEAAs compared to grasses. Among the legumes, A. hypogaea had the highest NEAA content (23.40%), while among the forages, C. zizanioides had the highest NEAA content (12.37%). However, it was observed that neither legumes nor grasses could fulfill the metabolizable TNEAAs requirements for gaining cattle (250 kg of empty body weight gain), unlike commercial concentrates, which were found to meet the requirements, especially for Arg, Glu, and thyronine. The provision of concentrate is necessary to supplement forage to meet the NEAA needs for cattle. [J Adv Vet Anim Res 2023; 10(4.000): 820-829]