This paper presents the results of molecular studies on testing and evaluation of multiplex test systems for the genetically modified organisms’ (GMOs) identification by PCR in real time. For the detection of genetic modifications in plants of the cabbage family (Brassicaceae) screening of major regulatory sequences is insufficient, as in genetic engineering manipulations 35S promoter – of DNA-containing cauliflower mosaic virus (CaMV) is used, which presence in the test material can lead to false positive results. In this work the feasibility of testing for the presence of viral CaMV’s DNA and analysis of screened gene sequences of interest for the usage during the analysis of rapeseed (Brassica napus L.) samples. It was established that the analysis of the presence/absence of a positive result for the CaMV 35S promoter is not giving complete answers to the absence of GMO in the sample. The appropriateness of GMO’s screening in rape seeds with NOS terminator and genes of interest.

This paper presents the results of molecular studies on testing and evaluation of multiplex test systems for the genetically modified organisms’ (GMOs) identification by PCR in real time. For the detection of genetic modifications in plants of the cabbage family (Brassicaceae) screening of major regulatory sequences is insufficient, as in genetic engineering manipulations 35S promoter – of DNA-containing cauliflower mosaic virus (CaMV) is used, which presence in the test material can lead to false positive results. In this work the feasibility of testing for the presence of viral CaMV’s DNA and analysis of screened gene sequences of interest for the usage during the analysis of rapeseed (Brassica napus L.) samples. It was established that the analysis of the presence/absence of a positive result for the CaMV 35S promoter is not giving complete answers to the absence of GMO in the sample. The appropriateness of GMO’s screening in rape seeds with NOS terminator and genes of interest.

100 rape varieties and hybrids of domestic and foreign selection that have been grown in the different soil-climatic zones of the country were evaluated. The paper presents the results of study by their biochemical characteristics: content of protein, oil and its fatty acid composition. High-oil varieties of rape are identifi ed with high content of erucic acid in oil – for the technical purposes and those without or with its low content – for food industry.

Colltction of haricot bean has been analyzed for origin and varieties have been classified for their vegetative period duration, saccharide content and yielding capacity.

Мета. Визначити біохімічні показники якості вирощуваного у різних умовах насіння сортів ріпаку озимого.Методи. У процесі досліджень використовували лабораторний, розрахунковий і статистичний методи, для підготовки висновків – аналізу та синтезу.Результати. За результатами лабораторних досліджень визначили біохімічні показники якості вирощуваного в різних умовах насіння сортів ріпаку озимого типу розвитку, а саме: вміст олії, «сирого протеїну» та глюкозинолатів. Значний вплив на показники якості насіння мають метеорологічні умови впродовж вегетаційного періоду рослин відповідної ґрунтово-кліматичної зони. Встановлено, що у 2022 р. біохімічні показники насіння ріпаку озимого були вищими ніж у 2020 р. Зростання урожайності на 1,6 т/га у зоні Степу, 1,1 т/га – Лісостепу, 1,4 т/га – Полісся забезпечило підвищення вмісту олії на 2,4, 6,6 та 6,7% відповідно. Збори олії з гектара збільшилися на 0,74 т/га у зонах Степу та Полісся, 0,62 т/га – Лісостепу.  У 2022 р., порівнюючи з 2020, спостерігали зниження показника «сирого протеїну»: на 3,1% у зоні Степу, 5,8% – Лісостепу та 5,0% – Полісся. На вміст глюкозинолатів впливали умови років досліджень у відповідних ґрунтово-кліматичних зонах. Степ і Лісостеп мали ідентичні показники – 0,7%, Полісся – 0,8%, тоді як у 2021 р. для зони Степу та Полісся вміст глюкозинолатів у насінні ріпаку був на 0,8 та 0,9% більшим, порівнюючи з показниками, отриманими в інші роки досліджень.Висновки. Встановлено, що в середньому за 2020–2022 рр. загальний вміст олії в насінні ріпаку становив 46,2% (Степ), 47,5% (Лісостеп) і 47,8% (Полісся); вміст «сирого протеїну» – 19,6% (Степ), 18,4% (Лісостеп) і 17,9% (Полісся); вміст глюкозинолатів – 0,7% (Степ та Лісостеп) і 0,8% (Полісся). Збір олії в зоні Степу мав показник 1,31 т/га, Лісостепу – 1,16 т/га, Полісся – 1,33 т/га. Збір білка з гектара становив 0,54 т/га для Степу, 0,44 т/га – Лісостепу та 0,48 т/га – Полісся. Умови вирощування за роки досліджень у відповідній зоні впливають на формування біохімічних показників насіння ріпаку озимого. | Purpose. To determine the biochemical indicators of seed quality of winter rape varieties grown under different conditions.Methods. During the research, the following methods were used: laboratory, calculation and statistical, to prepare conclusions – analysis and synthesis.Results. According to the results of laboratory studies, biochemical indicators of the quality of seeds of winter rape varieties, grown in different conditions, were established, namely: the content of oil, “crude protein” and glucosinolates. Meteorological conditions during the vegetation period of plants of the corresponding soil and climate zone have a significant influence on the quality indicators of seeds. Based on the results of the research, it was established that in 2022, compared to 2020, the biochemical indicators of winter rapeseed were higher. The increase in yield by 1.6 t/ha in the Steppe zone, 1.1 t/ha – Forest-Steppe, 1.4 t/ha – Polissia ensured an increase in oil content by 2.4% – Steppe, 6.6% – Forest-Steppe, 6.7% – Polissia and oil collection per hectare by 0.74 t/ha – Steppe and Polissia, 0.62 t/ha – Forest-Steppe. For the content of “crude protein”, a decrease in the index was observed, namely: by 3.1% in the Steppe zone, 5.8% in the Forest-Steppe and 5.0% in the Polissia in 2022 compared to 2020. Meteorological conditions of the research years in the corresponding soil and climatic zones had an influence on the content of glucosinolates. For the Steppe and Forest-Steppe zones, the indicators were identical and amounted to 0.7%, 0.8% (Polissia), while in 2021, for the Steppe and Polissia zones, the content of glucosinolates in rapeseed was 0.8% and 0.9% more, compared to the indicators obtained in other years of research.Conclusions. It was established that, on average, for 2020–2022, the total oil content in seeds of rape was 46.2% – Steppe, 47.5% – Forest-Steppe, 47.8% – Polissia; “crude protein” content – 19.6% – Steppe, 18.4% – Forest-Steppe, 17.9% – Polissia; the content of glucosinolates was 0.7% in the Steppe and Forest-steppe zones, 0.8% in the Polissia; oil collection in the Steppe zone 1.31 t/ha, Forest-Steppe – 1.16 t/ha, Polissia – 1.33 t/ha; protein collection per hectare is 0.54 t/ha in Steppe, 0.44 t/ha in Forest-Steppe and 0.48% in Polissia. Growing conditions over the years of research in the corresponding zone affect the formation of biochemical indicators of winter rapeseed.

Gaining a greater understanding of the plant microbiota and its interactions with its host plant heralds a new era of scientific discovery in agriculture. Different agricultural management practices influence soil microbial populations by changing a soil’s physical, chemical and biological properties. However, the impact of these practices on the microbiota associated with economically important crops such as oilseed rape, are still understudied. In this work we investigated the impact of two contrasting crop establishment practices, conventional (plow based) and conservation (strip–tillage) systems, on the microbiota inhabiting different plant microhabitats, namely rhizosphere, root and shoot, of winter oilseed rape under Irish agronomic conditions. Illumina 16S rRNA gene sequence profiling showed that the plant associated microhabitats (root and shoot), are dominated by members of the bacterial phyla Proteobacteria, Actinobacteria and Bacteroidetes. The root and shoot associated bacterial communities displayed markedly distinct profiles as a result of tillage practices. We observed a very limited ‘rhizosphere effect’ in the root zone of WOSR, i.e., there was little or no increase in bacterial community richness and abundance in the WOSR rhizosphere compared to the bulk soil. The two tillage systems investigated did not appear to lead to any major long term differences on the bulk soil or rhizosphere bacterial communities. Our data suggests that the WOSR root and shoot microbiota can be impacted by management practices and is an important mechanism that could allow us to understand how plants respond to different management practices and environments.