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Sampling for vegetative propagation: A phytosanitary status survey of grapevines collection by One Step RT-PCR method | Обстеження фітосанітарного статусу колекції винограду методом одностадійної ЗТ-ПЛР Texto completo
2021
Yzeiraj, M.
Purpose. Grapevines (Vitis spp.) are affected by many viral diseases which cause serious pathological problems. GLRaV-3 is among the most widespread leafroll viruses, while Grapevine Fanleaf Virus (GFLV) is a destructive pathogen which reduces the lifespan of grapevine. Considering the impact and the spread of these diseases, our objective was to analyse the presence of these two viruses in several grapevine varieties in grapevine collection at ATTC Vlore. Data gathered from plant pathogens serve to better understand and prevent the spread of pathogens, as a mandatory rule for the quality control of certified plant material during vegetative propagation. Method. The presence of two common viruses were tested using virus specific primers; LC1/LC2 primer pair designed from the hHSP70 gene for detecting Grapevine Leafroll-associated Virus-3 (GLRaV3) and C3390/H2999 primer pair, designed from coat protein coding regions for detecting Grapevine Fanleaf Virus (GFLV), in six varieties; ‘Merlot’, ‘Kallmet’, ‘Shesh i zi’, ‘Shesh i bardhё’, ‘Debinё’, and ‘Pulёz’, provided through a randomised sampling procedure. One Step Reverse Transcription Polymerase Chain Reaction assay was used to detect the viral presence. Results showed a high (100%) prevalence of GLRaV3 virus in all of analysed samples, as the most frequent among the two pathogens. Analysis for of GFLV virus showed low infection rate, being present in only one sample. Conclusions. We herein show an efficient, fast and reproducible method for detecting grapevine viruses through one step RT-PCR. Our results suggest that sampling of the infected plant material should be avoided due to the presence of viral infections. | Мета. Рослини винограду (Vitis spp.) уражуються багатьма вірусними збудниками, що спричиняють їхні серйозні захворювання. До найпоширеніших належать вірус скручування листя винограду (GLRaV-3) та вірус коротковузля винограду (GFLV), деструктивний патоген, який зменшує тривалість життя виноградної лози. З огляду на важливість і поширення захворювань, що спричиняються згаданими вірусами, нашою метою було проаналізувати їхню присутність у сортах винограду з колекції Центру Трансферу Агарних Технологій (ATTC Vlore). Отримані дані про рослинні патогени потрібні для запобігання їхньому поширенню і є обов’язковими для контролювання якості сертифікованого рослинного матеріалу під час його вегетативного розмноження. Методи. Наявність вірусів перевіряли методом одностадійної ЗТ-ПЛР з використанням вірус-специфічних праймерів: пара праймерів LC1 / LC2, що розроблена для детектування гена hHSP70 вірусу скручування листя винограду-3 (GLRaV3), і пара праймерів C3390 / H2999, для визначення кодувальних послідовностей білка оболонки вірусу коротковузля винограду (GFLV). Аналіз шести сортів культури – ‘Merlot’, ‘Kallmet’, ‘Shesh i zi’, ‘Shesh i bardhё’, ‘Debinё’ і ‘Pulёz’ – здійснювали з використанням процедури рандомізованої вибірки. Результати. Найпоширенішим вірусом для досліджених зразків виявився GLRaV3, який траплявся у 100% проаналізованих рослин. Визначення вірусу GFLV показало низький рівень інфікування, вірус був лише в одному зразку. Висновки. Показана можливість використання одностадійної ЗТ-ПЛР як ефективного, швидкого й відтворюваного методу виявлення вірусів виноградної лози.
Mostrar más [+] Menos [-]Foliar feeding of rootstocks of various cultivars of hazelnuts (Corylus maxima Mill.) in the mother plantation for horizontal vegetative propagation Texto completo
2015
Яремко, Н. О
Foliar feeding of rootstocks of various cultivars of hazelnuts (Corylus maxima Mill.) in the mother plantation for horizontal vegetative propagation Texto completo
2015
Яремко, Н. О
Purpose. Determination of optimum dozes of foliar feeding for each cultivar that will provide the highest output of standard rootstocks. Methods. Field, analytical and statistical ones. Results. The author presents the results of study of the hazelnut plant foliar feeding in the mother plantation for vegetative propagation with applying different carbamide concentration combined with 0.1% potassium sulfate (1.50.4 m) in case of horizontal method of growing. Biometric indices of hazelnut layers were analyzed; the influence of each factor on their height and diameter was determined. The rootstocks output per 1 linear meter for each cultivar as well as optimum foliar feeding doze was defined (0.5% carbamide with 0.1% potassium sulfate). Conclusions. The highest output of standard rootstocks in the mother plantation for horizontal vegetative propagation was achieved when applying the foliar feeding with 0.5% carbamide combined with 0.1% potassium sulfate, particularly (thousand rootstocks per 1 ha) for the cultivars: ‘Sviatkovyi’ – 66.7, ‘Dolynskyi’ – 62.1 and ‘Darunok Yunnatam’ – 50.7. For ‘Koronchatyi’ cultivar, the use of 3% carbamide was the most efficient.
Mostrar más [+] Menos [-]Foliar feeding of rootstocks of various cultivars of hazelnuts (Corylus maxima Mill.) in the mother plantation for horizontal vegetative propagation Texto completo
2015
Н. О. Яремко
Purpose. Determination of optimum dozes of foliar feeding for each cultivar that will provide the highest output of standard rootstocks. Methods. Field, analytical and statistical ones. Results. The author presents the results of study of the hazelnut plant foliar feeding in the mother plantation for vegetative propagation with applying different carbamide concentration combined with 0.1% potassium sulfate (1.50.4 m) in case of horizontal method of growing. Biometric indices of hazelnut layers were analyzed; the influence of each factor on their height and diameter was determined. The rootstocks output per 1 linear meter for each cultivar as well as optimum foliar feeding doze was defined (0.5% carbamide with 0.1% potassium sulfate). Conclusions. The highest output of standard rootstocks in the mother plantation for horizontal vegetative propagation was achieved when applying the foliar feeding with 0.5% carbamide combined with 0.1% potassium sulfate, particularly (thousand rootstocks per 1 ha) for the cultivars: ‘Sviatkovyi’ – 66.7, ‘Dolynskyi’ – 62.1 and ‘Darunok Yunnatam’ – 50.7. For ‘Koronchatyi’ cultivar, the use of 3% carbamide was the most efficient.
Mostrar más [+] Menos [-]Sampling for vegetative propagation: A phytosanitary status survey of grapevines collection by One Step RT-PCR method Texto completo
2021
Ізейрай, М
Sampling for vegetative propagation: A phytosanitary status survey of grapevines collection by One Step RT-PCR method Texto completo
2021
Ізейрай, М
Purpose. Grapevines (Vitis spp.) are affected by many viral diseases which cause serious pathological problems. GLRaV-3 is among the most widespread leafroll viruses, while Grapevine Fanleaf Virus (GFLV) is a destructive pathogen which reduces the lifespan of grapevine. Considering the impact and the spread of these diseases, our objective was to analyse the presence of these two viruses in several grapevine varieties in grapevine collection at ATTC Vlore. Data gathered from plant pathogens serve to better understand and prevent the spread of pathogens, as a mandatory rule for the quality control of certified plant material during vegetative propagation. Method. The presence of two common viruses were tested using virus specific primers; LC1/LC2 primer pair designed from the hHSP70 gene for detecting Grapevine Leafroll-associated Virus-3 (GLRaV3) and C3390/H2999 primer pair, designed from coat protein coding regions for detecting Grapevine Fanleaf Virus (GFLV), in six varieties; ‘Merlot’, ‘Kallmet’, ‘Shesh i zi’, ‘Shesh i bardhё’, ‘Debinё’, and ‘Pulёz’, provided through a randomised sampling procedure. One Step Reverse Transcription Polymerase Chain Reaction assay was used to detect the viral presence. Results showed a high (100%) prevalence of GLRaV3 virus in all of analysed samples, as the most frequent among the two pathogens. Analysis for of GFLV virus showed low infection rate, being present in only one sample. Conclusions. We herein show an efficient, fast and reproducible method for detecting grapevine viruses through one step RT-PCR. Our results suggest that sampling of the infected plant material should be avoided due to the presence of viral infections.
Mostrar más [+] Menos [-]?????????? ??????????????? ??????? ???????? ????????? ??????? ????????????? ??-??? | Sampling for vegetative propagation: A phytosanitary status survey of grapevines collection by One Step RT-PCR method Texto completo
???????, Yzeiraj, M., ?.
Purpose. Grapevines (Vitis spp.) are affected by many viral diseases which cause serious pathological problems. GLRaV-3 is among the most widespread leafroll viruses, while Grapevine Fanleaf Virus (GFLV) is a destructive pathogen which reduces the lifespan of grapevine. Considering the impact and the spread of these diseases, our objective was to analyse the presence of these two viruses in several grapevine varieties in grapevine collection at ATTC Vlore. Data gathered from plant pathogens serve to better understand and prevent the spread of pathogens, as a mandatory rule for the quality control of certified plant material during vegetative propagation. Method. The presence of two common viruses were tested using virus specific primers; LC1/LC2 primer pair designed from the hHSP70 gene for detecting Grapevine Leafroll-associated Virus-3 (GLRaV3) and C3390/H2999 primer pair, designed from coat protein coding regions for detecting Grapevine Fanleaf Virus (GFLV), in six varieties; ?Merlot?, ?Kallmet?, ?Shesh i zi?, ?Shesh i bardh??, ?Debin??, and ?Pul?z?, provided through a randomised sampling procedure. One Step Reverse Transcription Polymerase Chain Reaction assay was used to detect the viral presence. Results showed a high (100%) prevalence of GLRaV3 virus in all of analysed samples, as the most frequent among the two pathogens. Analysis for of GFLV virus showed low infection rate, being present in only one sample. Conclusions. We herein show an efficient, fast and reproducible method for detecting grapevine viruses through one step RT-PCR. Our results suggest that sampling of the infected plant material should be avoided due to the presence of viral infections. | ????. ??????? ????????? (Vitis spp.) ?????????? ???????? ????????? ??????????, ?? ??????????? ???? ???????? ????????????. ?? ?????????????? ???????? ????? ??????????? ????? ????????? (GLRaV-3) ?? ????? ???????????? ????????? (GFLV), ????????????? ???????, ???? ??????? ?????????? ????? ??????????? ????. ? ?????? ?? ?????????? ? ????????? ???????????, ?? ????????????? ????????? ????????, ????? ????? ???? ?????????????? ???? ??????????? ? ?????? ????????? ? ???????? ?????? ????????? ??????? ?????????? (ATTC Vlore). ???????? ???? ??? ???????? ???????? ???????? ??? ??????????? ??????? ????????? ? ? ????????????? ??? ????????????? ?????? ??????????????? ?????????? ????????? ??? ??? ???? ????????????? ???????????. ??????. ????????? ??????? ?????????? ??????? ????????????? ??-??? ? ????????????? ?????-??????????? ?????????: ???? ????????? LC1 / LC2, ?? ?????????? ??? ???????????? ???? hHSP70 ?????? ??????????? ????? ??????????-3 (GLRaV3), ? ???? ????????? C3390 / H2999, ??? ?????????? ??????????? ?????????????? ????? ???????? ?????? ???????????? ????????? (GFLV). ?????? ????? ?????? ???????? ? ?Merlot?, ?Kallmet?, ?Shesh i zi?, ?Shesh i bardh??, ?Debin?? ? ?Pul?z? ? ??????????? ? ????????????? ????????? ?????????????? ???????. ??????????. ?????????????? ??????? ??? ??????????? ??????? ???????? GLRaV3, ???? ?????????? ? 100% ??????????????? ??????. ?????????? ?????? GFLV ???????? ??????? ?????? ???????????, ????? ??? ???? ? ?????? ??????. ????????. ???????? ?????????? ???????????? ????????????? ??-??? ?? ???????????, ???????? ? ?????????????? ?????? ????????? ??????? ??????????? ????.
Mostrar más [+] Menos [-]Varieties of white cabbage and dіrections for their use Texto completo
2012
П. В. Дроща | Т. А. Данюк
The short description of varieties of white cabbage, the comparative analysis of the yield of cabbage and density of the cobs.
Mostrar más [+] Menos [-]Sampling for vegetative propagation: A phytosanitary status survey of grapevines collection by One Step RT-PCR method Texto completo
2021
М. Ізейрай
Sampling for vegetative propagation: A phytosanitary status survey of grapevines collection by One Step RT-PCR method Texto completo
2021
М. Ізейрай
Purpose. Grapevines (Vitis spp.) are affected by many viral diseases which cause serious pathological problems. GLRaV-3 is among the most widespread leafroll viruses, while Grapevine Fanleaf Virus (GFLV) is a destructive pathogen which reduces the lifespan of grapevine. Considering the impact and the spread of these diseases, our objective was to analyse the presence of these two viruses in several grapevine varieties in grapevine collection at ATTC Vlore. Data gathered from plant pathogens serve to better understand and prevent the spread of pathogens, as a mandatory rule for the quality control of certified plant material during vegetative propagation. Method. The presence of two common viruses were tested using virus specific primers; LC1/LC2 primer pair designed from the hHSP70 gene for detecting Grapevine Leafroll-associated Virus-3 (GLRaV3) and C3390/H2999 primer pair, designed from coat protein coding regions for detecting Grapevine Fanleaf Virus (GFLV), in six varieties; ‘Merlot’, ‘Kallmet’, ‘Shesh i zi’, ‘Shesh i bardhё’, ‘Debinё’, and ‘Pulёz’, provided through a randomised sampling procedure. One Step Reverse Transcription Polymerase Chain Reaction assay was used to detect the viral presence. Results showed a high (100%) prevalence of GLRaV3 virus in all of analysed samples, as the most frequent among the two pathogens. Analysis for of GFLV virus showed low infection rate, being present in only one sample. Conclusions. We herein show an efficient, fast and reproducible method for detecting grapevine viruses through one step RT-PCR. Our results suggest that sampling of the infected plant material should be avoided due to the presence of viral infections.
Mostrar más [+] Menos [-]Varietal diversity of walnuts in the Crimea and the perspective of its use in selection Texto completo
2012
Хохлов, С. Ю
The results of studying of walnuts collection have been given; the evaluation of the assortment according to resistance to unfavorable climatic conditions has been done; some varieties have been recommended for using in breeding and introduction in industry.
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