Purpose. To create a multiplex system for identification glyphosate-tolerant sugar beet by using PCR. Methods. Molecular genetic analysis. Results. The article presents the results of studies to determine the parameters of the polymerase chain reaction (PCR) in order to develop a multiplex system for identification of the structural elements of the design of transgenic gene cp 4 epsps, which provides tole­rance to glyphosate. For amplicon target DNA sequences, the following values of temperature conditions of PCR were determined: step 1 (initial denaturation) 95 °C – 3 min; step 2 (specific reaction products accumulation): denaturation 95 °C – 45 s; hybridization of primers 55 °C – 50 s; elongation 72 °C – 1 min; number of cycles – 40; step 3 (final elongation) 72 °C – 6 min. A series of PCR were carried out for the purpose of selecting the optimal amount of DNA matrix for efficient estimate of transgenic sugar beet plants for the presence of specific sequences. Conclusions. To identify transgenic glyphosate-tolerant sugar beet, it is advisable to determine 35S promoter and gene cp 4 epsps in individual genotypes. It was found that during the selection of temperature parameters of multiplex reaction a 5 °C rise in primer hybridization temperature did not affect the identification of gene als that allowed to include specific primers for determination of this sequence as an internal control. Based on the results of test multiplex reactions, concentrations of dNTPs and Mg2+ ions were determined that allowed to exclude the possibility of non-specific fragments and false-negative results. The optimum amount of matrix DNA (100–150 ng) for an efficient estimate of transgenic sugar beet plants for the presence of specific sequences was determined. Obtained results allowed to develop a multiplex test system for identification of transgenic glyphosate-to­lerant sugar beet which can be used for simultaneous determination of the 35S promoter, cp 4 epsps gene and als gene as an internal reaction control.

Purpose. To create a multiplex system for identification glyphosate-tolerant sugar beet by using PCR. Methods. Molecular genetic analysis. Results. The article presents the results of studies to determine the parameters of the polymerase chain reaction (PCR) in order to develop a multiplex system for identification of the structural elements of the design of transgenic gene cp 4 epsps, which provides tole­rance to glyphosate. For amplicon target DNA sequences, the following values of temperature conditions of PCR were determined: step 1 (initial denaturation) 95 °C – 3 min; step 2 (specific reaction products accumulation): denaturation 95 °C – 45 s; hybridization of primers 55 °C – 50 s; elongation 72 °C – 1 min; number of cycles – 40; step 3 (final elongation) 72 °C – 6 min. A series of PCR were carried out for the purpose of selecting the optimal amount of DNA matrix for efficient estimate of transgenic sugar beet plants for the presence of specific sequences. Conclusions. To identify transgenic glyphosate-tolerant sugar beet, it is advisable to determine 35S promoter and gene cp 4 epsps in individual genotypes. It was found that during the selection of temperature parameters of multiplex reaction a 5 °C rise in primer hybridization temperature did not affect the identification of gene als that allowed to include specific primers for determination of this sequence as an internal control. Based on the results of test multiplex reactions, concentrations of dNTPs and Mg2+ ions were determined that allowed to exclude the possibility of non-specific fragments and false-negative results. The optimum amount of matrix DNA (100–150 ng) for an efficient estimate of transgenic sugar beet plants for the presence of specific sequences was determined. Obtained results allowed to develop a multiplex test system for identification of transgenic glyphosate-to­lerant sugar beet which can be used for simultaneous determination of the 35S promoter, cp 4 epsps gene and als gene as an internal reaction control.

Purpose. To develop and improve adaptive technologies of winter wheat cultivation which provide high productivity and quality of grain. Methods. Field and laboratory stu­dies, mathematical and statistical analysis. Results. During 2011–2015, the impact of cultivation technologies on the formation of grain quality and yield of winter wheat varie­ty ‘Stolychna’ (with pea as predecessor) was studied. When using alternative technologies with only predecessor by-products application, the yield of winter wheat with integ­rated crop protection system was 4,56 t/ha, with minimal protection – 4.25 t/ha with grain quality of the 5th class of B group. Resource saving cultivation technologies with limi­ted application of fertilizers (Р45К45N30(II)+30(IV)) provided productivity at the level of 4,87–5,50 t/ha with grain qua­lity of the 2nd–3rd class of A group. Grain yield of 6.01 t/ha with indicators of the 2nd–3rd class of quality was obtained with the use of intensive cultivation technology with application of mineral fertilizers (Р90К90N30(II)+60(IV)+30(VIII)) on the background of applying predecessor’s by-products and integra­ted crop protection. The highest yield of grain (6.22 t/ha) with indicators of the 2nd class of A group quality on average for the research period was provided by energy-intensive technology, that requires the application of mineral fertilize­rs (P135K135N60(II)+75(IV)+45(VIII)) and incorporation of predecessor’s by-products in the soil, and integrated plant protection. Conclusion. It was found that in the northern part of the Right-Bank Forest-Steppe zone of Ukraine, the highest productivity of winter wheat was obtained in dark grey podzolic soils when using the energy-intensive technology with application of P135K135N60(II)+75(IV)+45(VIII) on the background of predecessor’s by-products and integrated crop protection. This technology ensured the grain yield of 6.22 t/ha of the 2nd class of A group quality.

Purpose. To develop and improve adaptive technologies of winter wheat cultivation which provide high productivity and quality of grain. Methods. Field and laboratory stu­dies, mathematical and statistical analysis. Results. During 2011–2015, the impact of cultivation technologies on the formation of grain quality and yield of winter wheat varie­ty ‘Stolychna’ (with pea as predecessor) was studied. When using alternative technologies with only predecessor by-products application, the yield of winter wheat with integ­rated crop protection system was 4,56 t/ha, with minimal protection – 4.25 t/ha with grain quality of the 5th class of B group. Resource saving cultivation technologies with limi­ted application of fertilizers (Р45К45N30(II)+30(IV)) provided productivity at the level of 4,87–5,50 t/ha with grain qua­lity of the 2nd–3rd class of A group. Grain yield of 6.01 t/ha with indicators of the 2nd–3rd class of quality was obtained with the use of intensive cultivation technology with application of mineral fertilizers (Р90К90N30(II)+60(IV)+30(VIII)) on the background of applying predecessor’s by-products and integra­ted crop protection. The highest yield of grain (6.22 t/ha) with indicators of the 2nd class of A group quality on average for the research period was provided by energy-intensive technology, that requires the application of mineral fertilize­rs (P135K135N60(II)+75(IV)+45(VIII)) and incorporation of predecessor’s by-products in the soil, and integrated plant protection. Conclusion. It was found that in the northern part of the Right-Bank Forest-Steppe zone of Ukraine, the highest productivity of winter wheat was obtained in dark grey podzolic soils when using the energy-intensive technology with application of P135K135N60(II)+75(IV)+45(VIII) on the background of predecessor’s by-products and integrated crop protection. This technology ensured the grain yield of 6.22 t/ha of the 2nd class of A group quality.

Purpose. Creation of experimental sugar beet hybrid combinations of high sugar yield values and defining gene­tic determination of their heterotic effect. Methods. Diallel crossing and topcrossing, genetic analysis of quantitative traits. Results. The authors have studied the frequency of occurrence of sugar beet heterotic hybrid combinations for «sugar yield» trait created on the basis of two pollinator lines to be genetically valuable for productivity elements, CMS lines and single-cross sterile hybrids with the use of diallel and topcrossing system of controlled hybridization. The share of parental components’ effect and their interaction in CMS hybrids variability for productivity was determined. Expediency of heterotic forecasting based on high combining ability lines was substabtiated. Promising high-yielding sugar beet combinations were selected that exceeded the group standard by 4.1–16.3%. Conclusions. The theory of genetic balance by M. V. Turbin was confirmed. Such hybrids as [CMS 5OT 4]MGP 1 (116.3%), [CMS 1OT 2]MGP 1 (112.5%) and [CMS 3OT 5]MGP 1 (113.2%) were recognized as the best for their productivity, MGP 1 and MGP 2 lines – as the best for their combining ability.

Purpose. Creation of experimental sugar beet hybrid combinations of high sugar yield values and defining gene­tic determination of their heterotic effect. Methods. Diallel crossing and topcrossing, genetic analysis of quantitative traits. Results. The authors have studied the frequency of occurrence of sugar beet heterotic hybrid combinations for «sugar yield» trait created on the basis of two pollinator lines to be genetically valuable for productivity elements, CMS lines and single-cross sterile hybrids with the use of diallel and topcrossing system of controlled hybridization. The share of parental components’ effect and their interaction in CMS hybrids variability for productivity was determined. Expediency of heterotic forecasting based on high combining ability lines was substabtiated. Promising high-yielding sugar beet combinations were selected that exceeded the group standard by 4.1–16.3%. Conclusions. The theory of genetic balance by M. V. Turbin was confirmed. Such hybrids as [CMS 5OT 4]MGP 1 (116.3%), [CMS 1OT 2]MGP 1 (112.5%) and [CMS 3OT 5]MGP 1 (113.2%) were recognized as the best for their productivity, MGP 1 and MGP 2 lines – as the best for their combining ability.

Historical review was done concerning the formation and development of scientific research of the Experimental Station of Medicinal Plants over the period 1916–2016. The main stages of experimenting in the direction of “medicinal plant cultivation’’ in Ukraine were covered. Role and importance of the Experimental Station of Medicinal Plants in the scientific and methodological support of such scientific directions as breeding, seed production, cultivation technologies, pharmacognosy in medicinal plant cultivation were shown. For the needs of the branch, over 100 species of introduced medicinal plants were studied at the Station and agricultu­ral techniques of their growing were developed, in addition, more than 50 varieties of medicinal plants were crea­ted, a number of machines and devices for growing, harves­ting and initial treatment of raw materials and seeds of the most important medicinal plants was designed. Other scientific achievements of the Station were mentioned and promising areas of medicinal plant research in Ukraine were outlined.

High-throughput sequencing (HTS) enables the generation of large amounts of genome sequence data at a reasonable cost. Organisms in mixed microbial communities can now be sequenced and identified in a culture-independent way, usually using amplicon sequencing of a DNA barcode. Bulk RNA-seq (metatranscriptomics) has several advantages over DNA-based amplicon sequencing: it is less susceptible to amplification biases, it captures only living organisms, and it enables a larger set of genes to be used for taxonomic identification. Using a model mock community comprising 17 fungal isolates, we evaluated whether metatranscriptomics can accurately identify fungal species and subspecies in mixed communities. Overall, 72.9% of the RNA transcripts were classified, from which the vast majority (99.5%) were correctly identified at the species level. Of the 15 species sequenced, 13 were retrieved and identified correctly. We also detected strain-level variation within the Cryptococcus species complexes: 99.3% of transcripts assigned to Cryptococcus were classified as one of the four strains used in the mock community. Laboratory contaminants and/or misclassifications were diverse, but represented only 0.44% of the transcripts. Hence, these results show that it is possible to obtain accurate species- and strain-level fungal identification from metatranscriptome data as long as taxa identified at low abundance are discarded to avoid false-positives derived from contamination or misclassifications. This study highlights both the advantages and current challenges in the application of metatranscriptomics in clinical mycology and ecological studies.

Purpose. To develop an algorithm of creation of new highly productive clonal varieties of Salicaceae family representatives with improved agronomic characters that can be used at energy plantations Methods. Field investigations, laboratory analysis, analytical approach, selection method. Results. Flow chart of selection process with representatives of Salicaceae family included the following stages: the Ist stage – creation of primary families (F0); the IInd stage – creation of the second families, or branches; the 222rd stage – interfamily (individually-familial) selection; IVth stage – familial-group selection; the Vth stage – clonal selection (selection of ramets). Conclusions. Breeding process with the use of complex of advanced methods of selection (individual, familial, group and clonal ones) allows to get model populations with high-frequency of valuable alleles and genotypes and with the high level of genetic variety. Due to a negative multi-stage individual intrafamilial and interfamily selection, cleaning of artificial populations from undesirable forms takes place without the substantial decrease of genetic variety level.

Purpose. To develop an algorithm of creation of new highly productive clonal varieties of Salicaceae family representatives with improved agronomic characters that can be used at energy plantations Methods. Field investigations, laboratory analysis, analytical approach, selection method. Results. Flow chart of selection process with representatives of Salicaceae family included the following stages: the Ist stage – creation of primary families (F0); the IInd stage – creation of the second families, or branches; the 222rd stage – interfamily (individually-familial) selection; IVth stage – familial-group selection; the Vth stage – clonal selection (selection of ramets). Conclusions. Breeding process with the use of complex of advanced methods of selection (individual, familial, group and clonal ones) allows to get model populations with high-frequency of valuable alleles and genotypes and with the high level of genetic variety. Due to a negative multi-stage individual intrafamilial and interfamily selection, cleaning of artificial populations from undesirable forms takes place without the substantial decrease of genetic variety level.

Purpose. Literature review of DNA banks creation as the current strategy of plant genetic resources conservation. Results. The current state of plant genetic resources conservation was analyzed in the context of the threat of gene­tic erosion. The importance of DNA banks was shown which function is to store DNA samples and associated products and disseminate them for research purposes. The main DNA banks in the world were described, including the Republican DNA Bank of Human, Animals, Plants and Microorganisms at the Institute of Genetics and Cytology of the National Academy of Sciences of Belarus. Stages of DNA banking were considered: tissue sampling (usually from leaves), cell destruction, DNA extraction, DNA storage. Different methods of tissue sampling, extraction and DNA storage were compared. The need for Plant DNA Bank creation in Ukraine was highlighted. Conclusions. DNA collections is an important resource in the global effort to overcome the crisis in biodiversity, for managing world genetic resources and maximi­zing their potential.

Purpose. Literature review of DNA banks creation as the current strategy of plant genetic resources conservation. Results. The current state of plant genetic resources conservation was analyzed in the context of the threat of gene­tic erosion. The importance of DNA banks was shown which function is to store DNA samples and associated products and disseminate them for research purposes. The main DNA banks in the world were described, including the Republican DNA Bank of Human, Animals, Plants and Microorganisms at the Institute of Genetics and Cytology of the National Academy of Sciences of Belarus. Stages of DNA banking were considered: tissue sampling (usually from leaves), cell destruction, DNA extraction, DNA storage. Different methods of tissue sampling, extraction and DNA storage were compared. The need for Plant DNA Bank creation in Ukraine was highlighted. Conclusions. DNA collections is an important resource in the global effort to overcome the crisis in biodiversity, for managing world genetic resources and maximi­zing their potential.

Purpose. Reviewing a history of formation and current state of the national nomenclature and streamlining the Ukrainian scientific species names for the genus Fragaria L. Results. Controversial attitudes towards the use of names «sunytsi» (= garden strawberry) and «polunytsi» (= hill strawberry) are inherently present in the Ukrainian nomenclature of the genus Fragaria L. The Ukrainian scientific names of species of this group of plants should be brought into line with the generic name “Sunytsi” in plural. Nothogeneric name x Fragophora Mezhenskyj (= Dasiphora Rafin. x Fragaria L.) is proposed. Conclusions. In a professional environment it is necessary to use only scientific names of species of the genus Fragaria – Sunytsi (= garden strawberry) that is used in plural in Ukrainian. Concerning garden strawberry, in every day life and popular literature the usage of a derivative sunytsia (in Ukrainian) in singular as well as polunytsia, polunytsi (in Ukrainian) may be permissible.

Purpose. Reviewing a history of formation and current state of the national nomenclature and streamlining the Ukrainian scientific species names for the genus Fragaria L. Results. Controversial attitudes towards the use of names «sunytsi» (= garden strawberry) and «polunytsi» (= hill strawberry) are inherently present in the Ukrainian nomenclature of the genus Fragaria L. The Ukrainian scientific names of species of this group of plants should be brought into line with the generic name “Sunytsi” in plural. Nothogeneric name x Fragophora Mezhenskyj (= Dasiphora Rafin. x Fragaria L.) is proposed. Conclusions. In a professional environment it is necessary to use only scientific names of species of the genus Fragaria – Sunytsi (= garden strawberry) that is used in plural in Ukrainian. Concerning garden strawberry, in every day life and popular literature the usage of a derivative sunytsia (in Ukrainian) in singular as well as polunytsia, polunytsi (in Ukrainian) may be permissible.

Purpose. Considering ways to solve issues related to soil cover study at the institutions for plant varieties examination. Methods.Fieldandanalytical ones. Results. Issues are identified that arise during soil sampling at institutions for plant varieties examination within the state system of plant varieties protection. The author considered in detail the current soil sampling techniques for agrochemical tes­ting for humus, nitrogen (compounds to be easily hydrolyzed), mobile P2O5, exchangeable K2O, hydrolytic acidity and others. Attention was paid to violation of methodological requirements concerning the time of soil sampling and number of samples for agrochemical testing at the experimental field, to the lack of devices for meteorological observation and appliances for soil and leaf diagnostics. The article also touches upon the issues associated with the study of soil cover at fields with scientifically-based crop rotation that belong to institutions for plant varieties examination within the state system of plant varieties protection. Conclusions. It should be necessary to follow recommended practice when sampling at elementary sites for agrochemical testing and performing all operations in accordance with crop cultivation flowcharts. General recommendations for agrochemical testing of fields in case of conventional crop rotations in farms are not suitable for fields with scientifically-based crop rotations at institutions for plant varieties examination.

Purpose. Considering ways to solve issues related to soil cover study at the institutions for plant varieties examination. Methods.Fieldandanalytical ones. Results. Issues are identified that arise during soil sampling at institutions for plant varieties examination within the state system of plant varieties protection. The author considered in detail the current soil sampling techniques for agrochemical tes­ting for humus, nitrogen (compounds to be easily hydrolyzed), mobile P2O5, exchangeable K2O, hydrolytic acidity and others. Attention was paid to violation of methodological requirements concerning the time of soil sampling and number of samples for agrochemical testing at the experimental field, to the lack of devices for meteorological observation and appliances for soil and leaf diagnostics. The article also touches upon the issues associated with the study of soil cover at fields with scientifically-based crop rotation that belong to institutions for plant varieties examination within the state system of plant varieties protection. Conclusions. It should be necessary to follow recommended practice when sampling at elementary sites for agrochemical testing and performing all operations in accordance with crop cultivation flowcharts. General recommendations for agrochemical testing of fields in case of conventional crop rotations in farms are not suitable for fields with scientifically-based crop rotations at institutions for plant varieties examination.

To analyse the modern classification and nomenclature of species of pome fruit crops which varieties are listed in the State Register of Plants Varieties Suitable for Dissemination in Ukraine, and improve terminological system of the Ukrainian names of both species and garden crops. Results. Fruit cultivars and most apple clonal rootstocks belong to Malus pumila, and ornamental cultivars belong to Malus gloriosa. The most common scientific name of the cultivated apple, especially among horticulturists, is Malus domestica, although according to the principle of priority the name Malus pumila should have the advantage. As far as Nomenclature Committee for Vascular Plants has rejected the proposal to conserve the name Malus domestica, Malus pumila is correct name for the cultivated apple. The use of synonymic name Malus domestica should be avoided in both scientific and scientific-popular papers for stability of nomenclature. Pear cultivars listed in the State Register of Plants Varieties Suitable for Dissemination in Ukraine are presented by Pyrus communis, and pear rootstocks – by Cydonia oblonga. Fruit cultivars of the latter belong to separate fruit crop named quince. An apple-quince hybrid was registered as universal clonal rootstock for pome fruit crops. The State Register of Plants Varieties Suitable for Dissemination in Ukraine also contains nonconventional fruit crops such as Chaenomeles and hawthorn that consist of some species and nothospecies. Conclusions. In scientific publications one should stop the use of synonymic name Malus domestica in favour of the correct name for cultivated apple Malus pumila. Apple, pears and Chaenomeles cultivars listed in the State Register of Plants Varieties Suitable for Dissemination in Ukraine have a complex multispecies origin whereas quince, hawthorn and pear roostock cultivars systematically are monospecies. A universal roootstock of pome fruit crops is Cydolus, or apple-quince, that resulted from interspecies hybridization between quince and apple. Refusal Refuse to use obsolete names of fruit plants species will promote harmonization of professional language.

To analyse the modern classification and nomenclature of species of pome fruit crops which varieties are listed in the State Register of Plants Varieties Suitable for Dissemination in Ukraine, and improve terminological system of the Ukrainian names of both species and garden crops. Results. Fruit cultivars and most apple clonal rootstocks belong to Malus pumila, and ornamental cultivars belong to Malus gloriosa. The most common scientific name of the cultivated apple, especially among horticulturists, is Malus domestica, although according to the principle of priority the name Malus pumila should have the advantage. As far as Nomenclature Committee for Vascular Plants has rejected the proposal to conserve the name Malus domestica, Malus pumila is correct name for the cultivated apple. The use of synonymic name Malus domestica should be avoided in both scientific and scientific-popular papers for stability of nomenclature. Pear cultivars listed in the State Register of Plants Varieties Suitable for Dissemination in Ukraine are presented by Pyrus communis, and pear rootstocks – by Cydonia oblonga. Fruit cultivars of the latter belong to separate fruit crop named quince. An apple-quince hybrid was registered as universal clonal rootstock for pome fruit crops. The State Register of Plants Varieties Suitable for Dissemination in Ukraine also contains nonconventional fruit crops such as Chaenomeles and hawthorn that consist of some species and nothospecies. Conclusions. In scientific publications one should stop the use of synonymic name Malus domestica in favour of the correct name for cultivated apple Malus pumila. Apple, pears and Chaenomeles cultivars listed in the State Register of Plants Varieties Suitable for Dissemination in Ukraine have a complex multispecies origin whereas quince, hawthorn and pear roostock cultivars systematically are monospecies. A universal roootstock of pome fruit crops is Cydolus, or apple-quince, that resulted from interspecies hybridization between quince and apple. Refusal Refuse to use obsolete names of fruit plants species will promote harmonization of professional language.

Purpose. To study morphometric parameters of fruits (cypselae) of purple coneflower (Echinacea purpurea (L.) Moench) of ‘Zirka Mykoly Vavylova’ cultivar and pale coneflower (Echinacea pallida (Nutt.) Nutt) of ‘Krasunia Prerii’ cultivar and determine the impact of agro-meteorological factors on their performance according to long-term data. Methods. Laboratory, Mathematics and Statistics. Results. It was found that the parameters of cypselae and its weight in various types of coneflowers varied considerably from year to year. In this context the indicators of cypselae width and thickness had a larger variability as compared with the length. Mean length of Echinacea purpurea ranged from 4,57 to 6,16 mm, width – 2,30–2,97 mm, thickness – 1,74–2,28 mm. Weight of a cypselae made up 4,40–6,50 mg. Length of a fruit of Echinacea pallida was 4,57–5,74 mm, width – 2,51–3,18 mm, thickness – 1,76–2,37 mm, and a fruit weight varied from 5,65 to 7,70 mg. The correlation analysis revealed reliable connection of agro-climatic parameters and the parameters of the fruit. Conclusions. Using long-term data, the morphology of cypselaes of two Echinacea species introduced to Ukraine were studied, their basic parameters and the variability of indicators, connection with agro-climatic factors that is necessary to consider in crop growing for seeds .

Purpose. To study morphometric parameters of fruits (cypselae) of purple coneflower (Echinacea purpurea (L.) Moench) of ‘Zirka Mykoly Vavylova’ cultivar and pale coneflower (Echinacea pallida (Nutt.) Nutt) of ‘Krasunia Prerii’ cultivar and determine the impact of agro-meteorological factors on their performance according to long-term data. Methods. Laboratory, Mathematics and Statistics. Results. It was found that the parameters of cypselae and its weight in various types of coneflowers varied considerably from year to year. In this context the indicators of cypselae width and thickness had a larger variability as compared with the length. Mean length of Echinacea purpurea ranged from 4,57 to 6,16 mm, width – 2,30–2,97 mm, thickness – 1,74–2,28 mm. Weight of a cypselae made up 4,40–6,50 mg. Length of a fruit of Echinacea pallida was 4,57–5,74 mm, width – 2,51–3,18 mm, thickness – 1,76–2,37 mm, and a fruit weight varied from 5,65 to 7,70 mg. The correlation analysis revealed reliable connection of agro-climatic parameters and the parameters of the fruit. Conclusions. Using long-term data, the morphology of cypselaes of two Echinacea species introduced to Ukraine were studied, their basic parameters and the variability of indicators, connection with agro-climatic factors that is necessary to consider in crop growing for seeds .