Те article reflects summary of hybridization and assessment of genetic value of pollen-sterile maternal lines of various origin and tetraploid pollinators produced by Bila Tserkva selection. High differential potential of pollinator-testers has been established. Best components exhibiting high combinative features have been detected, which served as a baseline for establishing MS (male sterile) hybrids of sugar beets.

Те article reflects summary of hybridization and assessment of genetic value of pollen-sterile maternal lines of various origin and tetraploid pollinators produced by Bila Tserkva selection. High differential potential of pollinator-testers has been established. Best components exhibiting high combinative features have been detected, which served as a baseline for establishing MS (male sterile) hybrids of sugar beets.

Varietal resources make one of the key national priorities. They pose themselves an outcome of intellectual effort by considerable society part - geneticists, breeders, physiologist, biochemists and biophysicists, immunologists and mathematicians, economists, ecologists and variety examiners.

Varietal resources make one of the key national priorities. They pose themselves an outcome of intellectual effort by considerable society part - geneticists, breeders, physiologist, biochemists and biophysicists, immunologists and mathematicians, economists, ecologists and variety examiners.

The article features results of studying F1 hybrids of tomato inheriting productivity factors. 21 original hybrid forms and 57 F1 tomato hybrids in green-house have been studied. The efficiency of selecting genotype of valuable plants by genotype is substantiated.

The article features results of studying F1 hybrids of tomato inheriting productivity factors. 21 original hybrid forms and 57 F1 tomato hybrids in green-house have been studied. The efficiency of selecting genotype of valuable plants by genotype is substantiated.

The article describes Agro-ecological features of winter triticale variety Vivate Nosivskyi developed by means of individual selection based on hybrid combination ([Avgusto x NE 312] x К 9844) at the Examination Facility of Nosivska Breeding and Testing Station ofChernigivIAPPIAAN and selected by quantitative (grain productivity, quantity and weight of grains per ear, cup weight of grain, 1000 kernels weight), qualitative (protein contain, «raw» gluten) parameters of grain yield, by adaptability to a range of causative agents, abnormal events in winter-spring and summer, lodging. It shows that bio-agents of microbe preparations Azospirillum brasilense и Achromobacter album 1122 on initial stages of development of winter triticale variety Vivate Nosivskyi plants enhance nitrogen and phosphor nutrition as reflected in raw and dry weight of plants increased by 1,5 to 2,5 times, area of leaf surface - 2 to 2,4 times, grain yield -1,1-1,3 times.

The article describes Agro-ecological features of winter triticale variety Vivate Nosivskyi developed by means of individual selection based on hybrid combination ([Avgusto x NE 312] x К 9844) at the Examination Facility of Nosivska Breeding and Testing Station ofChernigivIAPPIAAN and selected by quantitative (grain productivity, quantity and weight of grains per ear, cup weight of grain, 1000 kernels weight), qualitative (protein contain, «raw» gluten) parameters of grain yield, by adaptability to a range of causative agents, abnormal events in winter-spring and summer, lodging. It shows that bio-agents of microbe preparations Azospirillum brasilense и Achromobacter album 1122 on initial stages of development of winter triticale variety Vivate Nosivskyi plants enhance nitrogen and phosphor nutrition as reflected in raw and dry weight of plants increased by 1,5 to 2,5 times, area of leaf surface - 2 to 2,4 times, grain yield -1,1-1,3 times.

The review of legal regulation of state testing, registration of plant varieties based on genetically modified organisms in Ukraine and the European Union. Defined problems, namely the problem of harmonization of legislation, the actual Ukraine's lack of specific legislation in the area of biosafety for GMO handling, lack of clear procedures for registration of GMOs and others.

The review of legal regulation of state testing, registration of plant varieties based on genetically modified organisms in Ukraine and the European Union. Defined problems, namely the problem of harmonization of legislation, the actual Ukraine's lack of specific legislation in the area of biosafety for GMO handling, lack of clear procedures for registration of GMOs and others.

Breeding of columnar type apple varieties has been carried out at VNIISPK since 1984. The possibility of grow-ing of columnar type apple varieties in the crown of winter hardy semi-dwarf rootstock 3-4-98 by S. N. Stepanov’s   breeding is being studied in different density of planting   in the orchard (3 x 1,0 m; 3 x 1,5; and 3 x 2,0 m). The inves  tigation of winter hardiness, cropping power, fruit mar  ketability and biochemical composition allowed releasing two varieties «Poesia» and «Priokskoye» for state trials.

Breeding of columnar type apple varieties has been carried out at VNIISPK since 1984. The possibility of grow-ing of columnar type apple varieties in the crown of winter hardy semi-dwarf rootstock 3-4-98 by S. N. Stepanov’s breeding is being studied in different density of planting in the orchard (3 x 1,0 m; 3 x 1,5; and 3 x 2,0 m). The inves tigation of winter hardiness, cropping power, fruit mar ketability and biochemical composition allowed releasing two varieties «Poesia» and «Priokskoye» for state trials.

The article offers results of determination of labor input and predicted costs for qualifying examination of distinctness, uniformity and stability for varieties of the legumes. That will facilitate cost optimization and enable planning expenditures on scientific and planning basis.

True fungi (Fungi) and fungus-like organisms (e.g. Mycetozoa, Oomycota) constitute the second largest group of organisms based on global richness estimates, with around 3 million predicted species. Compared to plants and animals, fungi have simple body plans with often morphologically and ecologically obscure structures. This poses challenges for accurate and precise identifications. Here we provide a conceptual framework for the identification of fungi, encouraging the approach of integrative (polyphasic) taxonomy for species delimitation, i.e. the combination of genealogy (phylogeny), phenotype (including autecology), and reproductive biology (when feasible). This allows objective evaluation of diagnostic characters, either phenotypic or molecular or both. Verification of identifications is crucial but often neglected. Because of clade-specific evolutionary histories, there is currently no single tool for the identification of fungi, although DNA barcoding using the internal transcribed spacer (ITS) remains a first diagnosis, particularly in metabarcoding studies. Secondary DNA barcodes are increasingly implemented for groups where ITS does not provide sufficient precision. Issues of pairwise sequence similarity-based identifications and OTU clustering are discussed, and multiple sequence alignment-based phylogenetic approaches with subsequent verification are recommended as more accurate alternatives. In metabarcoding approaches, the trade-off between speed and accuracy and precision of molecular identifications must be carefully considered. Intragenomic variation of the ITS and other barcoding markers should be properly documented, as phylotype diversity is not necessarily a proxy of species richness. Important strategies to improve molecular identification of fungi are: (1) broadly document intraspecific and intragenomic variation of barcoding markers; (2) substantially expand sequence repositories, focusing on undersampled clades and missing taxa; (3) improve curation of sequence labels in primary repositories and substantially increase the number of sequences based on verified material; (4) link sequence data to digital information of voucher specimens including imagery. In parallel, technological improvements to genome sequencing offer promising alternatives to DNA barcoding in the future. Despite the prevalence of DNA-based fungal taxonomy, phenotype-based approaches remain an important strategy to catalog the global diversity of fungi and establish initial species hypotheses.

True fungi (Fungi) and fungus-like organisms (e.g. Mycetozoa, Oomycota) constitute the second largest group of organisms based on global richness estimates, with around 3 million predicted species. Compared to plants and animals, fungi have simple body plans with often morphologically and ecologically obscure structures. This poses challenges for accurate and precise identifications. Here we provide a conceptual framework for the identification of fungi, encouraging the approach of integrative (polyphasic) taxonomy for species delimitation, i.e. the combination of genealogy (phylogeny), phenotype (including autecology), and reproductive biology (when feasible). This allows objective evaluation of diagnostic characters, either phenotypic or molecular or both. Verification of identifications is crucial but often neglected. Because of clade-specific evolutionary histories, there is currently no single tool for the identification of fungi, although DNA barcoding using the internal transcribed spacer (ITS) remains a first diagnosis, particularly in metabarcoding studies. Secondary DNA barcodes are increasingly implemented for groups where ITS does not provide sufficient precision. Issues of pairwise sequence similarity-based identifications and OTU clustering are discussed, and multiple sequence alignment-based phylogenetic approaches with subsequent verification are recommended as more accurate alternatives. In metabarcoding approaches, the trade-off between speed and accuracy and precision of molecular identifications must be carefully considered. Intragenomic variation of the ITS and other barcoding markers should be properly documented, as phylotype diversity is not necessarily a proxy of species richness. Important strategies to improve molecular identification of fungi are: (1) broadly document intraspecific and intragenomic variation of barcoding markers; (2) substantially expand sequence repositories, focusing on undersampled clades and missing taxa; (3) improve curation of sequence labels in primary repositories and substantially increase the number of sequences based on verified material; (4) link sequence data to digital information of voucher specimens including imagery. In parallel, technological improvements to genome sequencing offer promising alternatives to DNA barcoding in the future. Despite the prevalence of DNA-based fungal taxonomy, phenotype-based approaches remain an important strategy to catalog the global diversity of fungi and establish initial species hypotheses.

The short description of varieties of white cabbage, the comparative analysis of the yield of cabbage and density of the cobs.

Purpose. Grapevines (Vitis spp.) are affected by many viral diseases which cause serious pathological problems. GLRaV-3 is among the most widespread leafroll viruses, while Grapevine Fanleaf Virus (GFLV) is a destructive pathogen which reduces the lifespan of grapevine. Considering the impact and the spread of these diseases, our objective was to analyse the presence of these two viruses in several grapevine varieties in grapevine collection at ATTC Vlore. Data gathered from plant pathogens serve to better understand and prevent the spread of pathogens, as a mandatory rule for the quality control of certified plant material during vegetative propagation. Method. The presence of two common viruses were tested using virus specific primers; LC1/LC2 primer pair designed from the hHSP70 gene for detecting Grapevine Leafroll-associated Virus-3 (GLRaV3) and C3390/H2999 primer pair, designed from coat protein coding regions for detecting Grapevine Fanleaf Virus (GFLV), in six varieties; ?Merlot?, ?Kallmet?, ?Shesh i zi?, ?Shesh i bardh??, ?Debin??, and ?Pul?z?, provided through a randomised sampling procedure. One Step Reverse Transcription Polymerase Chain Reaction assay was used to detect the viral presence. Results showed a high (100%) prevalence of GLRaV3 virus in all of analysed samples, as the most frequent among the two pathogens. Analysis for of GFLV virus showed low infection rate, being present in only one sample. Conclusions. We herein show an efficient, fast and reproducible method for detecting grapevine viruses through one step RT-PCR. Our results suggest that sampling of the infected plant material should be avoided due to the presence of viral infections. | ????. ??????? ????????? (Vitis spp.) ?????????? ???????? ????????? ??????????, ?? ??????????? ???? ???????? ????????????. ?? ?????????????? ???????? ????? ??????????? ????? ????????? (GLRaV-3) ?? ????? ???????????? ????????? (GFLV), ????????????? ???????, ???? ??????? ?????????? ????? ??????????? ????. ? ?????? ?? ?????????? ? ????????? ???????????, ?? ????????????? ????????? ????????, ????? ????? ???? ?????????????? ???? ??????????? ? ?????? ????????? ? ???????? ?????? ????????? ??????? ?????????? (ATTC Vlore). ???????? ???? ??? ???????? ???????? ???????? ??? ??????????? ??????? ????????? ? ? ????????????? ??? ????????????? ?????? ??????????????? ?????????? ????????? ??? ??? ???? ????????????? ???????????. ??????. ????????? ??????? ?????????? ??????? ????????????? ??-??? ? ????????????? ?????-??????????? ?????????: ???? ????????? LC1 / LC2, ?? ?????????? ??? ???????????? ???? hHSP70 ?????? ??????????? ????? ??????????-3 (GLRaV3), ? ???? ????????? C3390 / H2999, ??? ?????????? ??????????? ?????????????? ????? ???????? ?????? ???????????? ????????? (GFLV). ?????? ????? ?????? ???????? ? ?Merlot?, ?Kallmet?, ?Shesh i zi?, ?Shesh i bardh??, ?Debin?? ? ?Pul?z? ? ??????????? ? ????????????? ????????? ?????????????? ???????. ??????????. ?????????????? ??????? ??? ??????????? ??????? ???????? GLRaV3, ???? ?????????? ? 100% ??????????????? ??????. ?????????? ?????? GFLV ???????? ??????? ?????? ???????????, ????? ??? ???? ? ?????? ??????. ????????. ???????? ?????????? ???????????? ????????????? ??-??? ?? ???????????, ???????? ? ?????????????? ?????? ????????? ??????? ??????????? ????.

Purpose. Grapevines (Vitis spp.) are affected by many viral diseases which cause serious pathological problems. GLRaV-3 is among the most widespread leafroll viruses, while Grapevine Fanleaf Virus (GFLV) is a destructive pathogen which reduces the lifespan of grapevine. Considering the impact and the spread of these diseases, our objective was to analyse the presence of these two viruses in several grapevine varieties in grapevine collection at ATTC Vlore. Data gathered from plant pathogens serve to better understand and prevent the spread of pathogens, as a mandatory rule for the quality control of certified plant material during vegetative propagation. Method. The presence of two common viruses were tested using virus specific primers; LC1/LC2 primer pair designed from the hHSP70 gene for detecting Grapevine Leafroll-associated Virus-3 (GLRaV3) and C3390/H2999 primer pair, designed from coat protein coding regions for detecting Grapevine Fanleaf Virus (GFLV), in six varieties; ‘Merlot’, ‘Kallmet’, ‘Shesh i zi’, ‘Shesh i bardhё’, ‘Debinё’, and ‘Pulёz’, provided through a randomised sampling procedure. One Step Reverse Transcription Polymerase Chain Reaction assay was used to detect the viral presence. Results showed a high (100%) prevalence of GLRaV3 virus in all of analysed samples, as the most frequent among the two pathogens. Analysis for of GFLV virus showed low infection rate, being present in only one sample. Conclusions. We herein show an efficient, fast and reproducible method for detecting grapevine viruses through one step RT-PCR. Our results suggest that sampling of the infected plant material should be avoided due to the presence of viral infections.