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Cadmium exposure induces osteoporosis through cellular senescence, associated with activation of NF-κB pathway and mitochondrial dysfunction
2021
Luo, Huigen | Gu, Renjie | Ouyang, Huiya | Wang, Lihong | Shi, Shanwei | Ji, Yuna | Bao, Baicheng | Liao, Guiqing | Xu, Baoshan
Cadmium (Cd) is a heavy metal toxicant as a common pollutant derived from many agricultural and industrial sources. The absorption of Cd takes place primarily through Cd-contaminated food and water and, to a significant extent, via inhalation of Cd-contaminated air and cigarette smoking. Epidemiological data suggest that occupational or environmental exposure to Cd increases the health risk for osteoporosis and spontaneous fracture such as itai-itai disease. However, the direct effects and underlying mechanism(s) of Cd exposure on bone damage are largely unknown. We used primary bone marrow-derived mesenchymal stromal cells (BMMSCs) and found that Cd significantly induced BMMSC cellular senescence through over-activation of NF-κB signaling pathway. Increased cell senescence was determined by production of senescence-associated secretory phenotype (SASP), cell cycle arrest and upregulation of p21/p53/p16ᴵᴺᴷ⁴ᵃ protein expression. Additionally, Cd impaired osteogenic differentiation and increased adipogenesis of BMMSCs, and significantly induced cellular senescence-associated defects such as mitochondrial dysfunction and DNA damage. Sprague-Dawley (SD) rats were chronically exposed to Cd to verify that Cd significantly increased adipocyte number, and decreased mineralization tissues of bone marrow in vivo. Interestingly, we observed that Cd exposure remarkably retarded bone repair and regeneration after operation of skull defect. Notably, pretreatment of melatonin is able to partially prevent Cd-induced some senescence-associated defects of BMMSCs including mitochondrial dysfunction and DNA damage. Although Cd activated mammalian target of rapamycin (mTOR) pathway, rapamycin only partially ameliorated Cd-induced cell apoptosis rather than cellular senescence phenotypes of BMMSCs. In addition, a selective NF-κB inhibitor moderately alleviated Cd-caused the senescence-related defects of the BMMSCs. The study shed light on the action and mechanism of Cd on osteoporosis and bone ageing, and may provide a novel option to ameliorate the harmful effects of Cd exposure.
Mostrar más [+] Menos [-]Exploring applicability of end member mixing approach for predicting environmental reactivity of dissolved organic matter
2021
Tak, Surbhi | Han, So-Jeong | Lee, Yun-Kyung | Cho, Jinwoo | Hur, Jin
Despite the wide applications of end member mixing analysis (EMMA) for assigning the sources of dissolved organic matter (DOM) in aquatic environment, there was no study attempting to test the applicability of EMMA for predicting environmental reactivity of DOM. This study aimed to explore the feasibility of EMMA, or the concept of ideal mixing behavior of end members, for describing several well-known DOM reactivities using two DOM end member sources (i.e., soil and algae) at varying mixing ratios. The selected DOM reactivities were trihalomethane formation potential (THMFP), mineral adsorption amount, pyrene binding, membrane resistance, and biodegradation potential. Among the tested DOM functions, all were found to follow the ideal mixing behavior, presenting the linear relationships between the source mixing ratios and the tested reactivity with the R² value of >0.80. The ideal mixing behavior of the DOM functions was more pronounced than that based on several spectroscopic indicators derived from UV absorption and fluorescence spectroscopy. This study provided insight into potential applicability and limitation of EMMA approach in monitoring and predicting environmental functions of DOM in aquatic systems where identified DOM sources are mixed and vary dynamically with the mixing ratios.
Mostrar más [+] Menos [-]Estimating NOx removal capacity of urban trees using stable isotope method: A case study of Beijing, China
2021
Gong, Cheng | Xian, Chaofan | Cui, Bowen | He, Guojin | Wei, Mingyue | Zhang, Zhaoming | Ouyang, Z. (Zhiyun)
It is widely recognized that green infrastructures in urban ecosystems provides important ecosystem services, including air purification. The potential absorption of nitrogen oxides (NOₓ) by urban trees has not been fully quantified, although it is important for air pollution mitigation and the well-being of urban residents. In this study, four common tree species (Sophora japonica L., Fraxinus chinensis Roxb., Populus tomentosa Carrière, Sabina chinensis (L.)) in Beijing, China, were studied. The dual stable isotopes (¹⁵N and ¹⁸O) and a Bayesian isotope mixing model were applied to estimate the sources contributions of potential nitrogen sources to the roadside trees based on leaf and soil sampling in urban regions. The following order of sources contributions was determined: soil > dry deposition > traffic-related NOₓ. The capacity of urban trees for NOₓ removal in the city was estimated using a remote sensing and GIS approach, and the removal capacity was found to range from 0.79 to 1.11 g m⁻² a⁻¹ across administrative regions, indicating that 1304 tons of NOₓ could be potentially removed by urban trees in 2019. Our finding qualified the potential NOₓ removal by urban trees in terms of atmospheric pollution mitigation, highlighting the role of green infrastructure in air purification, which should be taken into account by stakeholders to manage green infrastructure as the basis of a nature-based approach.
Mostrar más [+] Menos [-]Ammonium-nitrogen addition at the seedling stage does not reduce grain cadmium concentration in two common wheat (Triticum aestivum L.) cultivars
2021
Cheng, Yiran | Yang, Tian | Xiang, Wenhui | Li, Siyu | Fan, Xing | Sha, Lina | Kang, Houyang | Wu, Dandan | Zhang, Haiqin | Zeng, Jian | Zhou, Yonghong | Wang, Yi
High cadmium (Cd) concentration in common wheat (Triticum aestivum L.) grains poses potential health risks. Several management strategies have been used to reduce grain Cd concentration. However, limited information is available on the use of ammonium-nitrogen (NH₄⁺-N) as a strategy to manage Cd concentration in wheat grains. In this study, NH₄⁺-N addition at the seedling stage unchanged the grain Cd concentration in the high-Cd accumulator, Zhoumai 18 (ZM18), but dramatically increased that in the low-Cd accumulator, Yunmai 51 (YM51). Further analysis revealed that the effects of NH₄⁺-N addition on whole-plant Cd absorption, root-to-shoot Cd translocation, and shoot-to-grain Cd remobilization were different between the two wheat cultivars. In ZM18, NH₄⁺-N addition did not change whole-plant Cd absorption, but inhibited root-to-shoot Cd translocation and Cd remobilization from lower internodes, lower leaves, node 1, and internode 1 to grains via the down-regulation of yellow stripe-like transporters (YSL), zinc transporters (ZIP5, ZIP7, and ZIP10), and heavy-metal transporting ATPases (HMA2). This inhibition decreased the grain Cd content by 29.62%, which was consistent with the decrease of the grain dry weight by 23.26%, leading to unchanged grain Cd concentration in ZM18. However, in YM51, NH₄⁺-N addition promoted continuous Cd absorption during grain filling, root-to-shoot Cd translocation and whole-plant Cd absorption. The absorbed Cd was directly transported to internode 1 via the xylem and then re-transported to grains via the phloem by up-regulated YSL, ZIP5, and copper transporters (COPT4). This promotion increased the grain Cd content by 245.35%, which was higher than the increased grain dry weight by 132.89%, leading to increased grain Cd concentration in YM51. Our findings concluded that the addition of NH₄⁺-N fertilizer at the seedling stage is not suitable for reducing grain Cd concentration in common wheat cultivars.
Mostrar más [+] Menos [-]Comprehensive evaluation of ionic liquid [Bmim][PF6] for absorbing toluene and acetone
2021
Ma, Xiaoling | Wang, Wenlong | Sun, Chenggong | Sun, Jing
Absorption is an eminent technology for volatile organic compounds (VOCs) elimination with the merits of high efficiency and low cost. Absorbent plays a critical role in the absorption process, and the thermal stability, saturation capacity, and regeneration performance should be concerned. As a kind of green and eco-friendly solvent, ionic liquid (IL) is expected to be a substitute for the conventional VOCs absorbent. In this study, 1-butyl-3-methylimidazolium hexafluorophosphate ([Bmim][PF₆]) is employed to absorb the modeling VOCs (toluene and acetone). Moreover, the used [Bmim][PF₆] is recovered by thermal distillation and the reusability is then conducted by consecutive batch experiments. Based on that, the thermal stability of [Bmim][PF₆] is comprehensively examined, in which the kinetic and thermodynamic parameters are also calculated. Results reveal that [Bmim][PF₆] owned promising toluene absorption performance with inlet concentration of 3000 mg/m³ and flow rate of 300 mL/min at 20 °C, it possesses the saturated adsorption capacity of 5.16 mg/g. [Bmim][PF₆] also shows satisfying thermal stability up to 610 K. In addition, thermal distillation is proved to be a reliable regeneration route on account of the recovered [Bmim][PF₆] remained satisfying capacity even after five cycles.
Mostrar más [+] Menos [-]Effect of source variation on the size and mixing state of black carbon aerosol in urban Beijing from 2013 to 2019: Implication on light absorption
2021
Wu, Yunfei | Xia, Yunjie | Wing, Omar | Tian, Ping | Tao, Jun | Huang, Ru-Jin | Liu, Dantong | Wang, Xin | Xia, Xiangao | Han, Zhiwei | Zhang, Renjian
Black carbon (BC) is the most important aerosol light-absorbing component, and its effect on radiation forcing is determined by its microphysical properties. In this study, two microphysical parameters of refractory BC (rBC), namely, size distribution and mixing state, in urban Beijing from 2013 to 2019 were investigated to understand the effects of source changes over the past years. The mass equivalent diameter of rBC (Dc) exhibited bimodal lognormal distributions in all seasons, with the major modes accounting for most (>85%) of the rBC masses. The mass median diameter (MMD) was obviously larger in winter (209 nm) than in summer (167 nm) likely due to the contribution of more rBC with larger Dc from solid fuel combustion and enhanced coagulation of rBC in polluted winter. More rBC particles were thickly coated in winter, with the number fraction of thickly coated rBC (fcₒₐₜBC) ranging within 29%–48% compared with that of 12%–14% in summer. However, no evidential increase in BC light-absorption capability was observed in winter. This finding was likely related to the lower absorption efficiency of larger rBC in winter, which partly offset the coating-induced light enhancement. Two stage of decreases in MMD and fcₒₐₜBC were observed, accompanied with a persistent decrease in rBC loading, thereby reflecting the discrepant effects of source control measures on rBC loading and physical properties. The control measures in the earlier stage before 2016 was more efficient to reduce the rBC loading but slightly influenced the microphysical properties of rBC. As of 2016, the reduction in rBC concentration slowed down because of its low atmospheric loading. However, rBC showed a more obvious decrease in its core size and became less coated. The decrease in fcₒₐₜBC may have weakened the BC absorption and accelerated the decrease in light absorption resulting from the reduction in rBC loading.
Mostrar más [+] Menos [-]Characterization of allergenicity of Platanus pollen allergen a 3 (Pla a 3) after exposure to NO2 and O3
2021
Zhou, Shumin | Wang, Xingzi | Lu, Senlin | Yao, Chuanhe | Zhang, Luying | Rao, Lanfang | Liu, Xinchun | Zhang, Wei | Li, Shuijun | Wang, Weiqian | Wang, Qingyue
Pollen allergens, widely present in the atmosphere, are the main cause of seasonal respiratory diseases that affect millions of people worldwide. Although previous studies have reported that nitrogen dioxide (NO₂) and ozone (O₃) promote pollen allergy, the specific biological processes and underlying mechanisms remain less understood. In this study, Platanus pollen grains were exposed to gaseous pollutants (NO₂ and O₃). We employed environmental electron microscopy, flow cytometry, western blot assay, enzyme-linked immunoassay, ultraviolet absorption spectrometry, circular dichroism, and protein mass spectrometry to characterise the subpollen particles (SPPs) released from pollen grains. Furthermore, we determined the immunogenicity and pathogenicity induced by Platanus pollen allergen a 3 (Pla a 3). Our results demonstrated that NO₂ and O₃ could damage the pollen cell membranes in SPPs and increase the amount of Pla a 3 allergen released into the atmosphere. Additionally, NO₂ and O₃ altered the structure of Pla a3 protein through nitrification and oxidation, which not only enhanced the immunogenicity of allergens but also increased the stability of the protein. In vivo analysis using an animal model indicated that NO₂ and O₃ greatly aggravated pollen-induced pneumonia. Thus, our study provides guidance for the prevention of pollen allergic diseases.
Mostrar más [+] Menos [-]Nontarget analysis reveals gut microbiome-dependent differences in the fecal PCB metabolite profiles of germ-free and conventional mice
2021
Li, Xueshu | Liu, Yanna | Martin, Jonathan W. | Cui, Julia Yue | Lehmler, Hans-Joachim
Mammalian polychlorinated biphenyl (PCB) metabolism has not been systematically explored with nontarget high-resolution mass spectrometry (Nt-HRMS). Here we investigated the importance of the gut microbiome in PCB biotransformation by Nt-HRMS analysis of feces from conventional (CV) and germ-free (GF) adult female mice exposed to a single oral dose of an environmental PCB mixture (6 mg/kg or 30 mg/kg in corn oil). Feces were collected for 24 h after PCB administration, PCB metabolites were extracted from pooled samples, and the extracts were analyzed by Nt-HRMS. Twelve classes of PCB metabolites were detected in the feces from CV mice, including PCB sulfates, hydroxylated PCB sulfates (OH-PCB sulfates), PCB sulfonates, and hydroxylated methyl sulfone PCBs (OH-MeSO₂-PCBs) reported previously. We also observed eight additional PCB metabolite classes that were tentatively identified as hydroxylated PCBs (OH-PCBs), dihydroxylated PCBs (DiOH-PCBs), monomethoxylated dihydroxylated PCBs (MeO-OH-PCBs), methoxylated PCB sulfates (MeO-PCB sulfates), mono-to tetra-hydroxylated PCB quinones ((OH)ₓ-quinones, x = 1–4), and hydroxylated polychlorinated benzofurans (OH-PCDF). Most metabolite classes were also detected in the feces from GF mice, except for MeO-OH-PCBs, OH-MeSO₂-PCBs, and OH-PCDFs. Semi-quantitative analyses demonstrate that relative PCB metabolite levels increased with increasing dose and were higher in CV than GF mice, except for PCB sulfates and MeO-PCB sulfates, which were higher in GF mice. These findings demonstrate that the gut microbiome plays a direct or indirect role in the absorption, distribution, metabolism, or excretion of PCB metabolites, which in turn may affect toxic outcomes following PCB exposure.
Mostrar más [+] Menos [-]Synthesis and performance evaluation of plastic waste aerogel as sustainable and reusable oil absorbent
2021
Pawar, Atul A. | Kim, Ayoung | Kim, Hern
Direct utilization of waste polyethylene terephthalate (PET) from the environment to form highly porous aerogel technology for oil absorption is an attractive approach from the view point of green chemistry. However, the oil absorption reaction is limited by low oil absorption capacity and less stability. For now, silica aerogel are used to solve these problem. Our goal is to substitute to these silica aerogel with PET aerogel technology. Herein, we have prepared an environmental waste PET based aerogel with 1.0:0.5 wt% PET, polyvinyl alcohol (PVA), and glutaraldehyde (GA) 0.2% v/v were dispersed in 10 mL DI water, followed by homogenization (30 min), sonication (10 min), and ageing (2 h) at 70 °C. To escape macroscopic cracking, cooling (8 h) at 4 °C was followed by freezing (6 h), freeze drying at −80 °C, and 5 mTorr for 18 h. The hybrid PET aerogel displays excellent performance towards oil absorption. Notably it showed high absorption capacity towards the different oils about 21–40 times its own weight, depending on the viscosity and density of the oil and solvents within 15–35 s, 25 °C, and 2 × 2 cm aerogel size. In addition, the aerogel shows there is no change in structure after several recycles due to high mechanical strength. Furthermore, because of the PET aerogel's high porosity (99.74%) and low density (0.0311 g/cm³), close bonding between PET-PVA occurs. Therefore, aerogel shows hydrophobic nature, good mechanical strength, high thermal stability, arrangement of the interconnected fibrillar pore network offers a high surface to volume ratio, low surface energy, high surface roughness, and more reusability. All these parameters are responsible for high oil absorption.
Mostrar más [+] Menos [-]Visible light driven exotic p (CuO) - n (TiO2) heterojunction for the photodegradation of 4-chlorophenol and antibacterial activity
2021
Gnanasekaran, Lalitha | Pachaiappan, Rekha | Kumar, P Senthil | Hoang, Tuan K.A. | Rajendran, Saravanan | Durgalakshmi, D. | Soto-Moscoso, Matias | Cornejo-Ponce, Lorena | Gracia, F.
The treatment of industrial waste and harmful bacteria is an important topic due to the release of toxins from the industrial pollutants that damage the water resources. These harmful sources frighten the life of every organism which was later developed as the carcinogenic and mutagenic agents. Therefore, the current study focuses on the breakdown or degradation of 4-chlorophenol and the antibacterial activity against Escherichia coli (E. coli). As a well-known catalyst, pure titanium-di-oxide (TiO₂) had not shown the photocatalytic activity in the visible light region. Hence, band position of TiO₂ need to be shifted to bring out the absorption in the visible light region. For this purpose, the n-type TiO₂ nanocrystalline material's band gap got varied by adding different ratios of p-type CuO. The result had appeared in the formation of p (CuO) – n (TiO₂) junction synthesized from sol-gel followed by chemical precipitation methods. The optical band gap value was determined by Kubelka-Munk (K-M) plot through UV–Vis diffusive reflectance spectroscopy (DRS). Further, the comprehensive mechanism and the results of photocatalytic and antibacterial activities were discussed in detail. These investigations are made for tuning the TiO₂ catalyst towards improving or eliminating the existing various environmental damages.
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