The agricultural sector uses fertilizers such as urea to add more nutrients to the soil needed for plant growth. Although it is cost-effective in crop production, indiscriminate use of nitrate-based fertilizer may result in behavioural, morphological, and physiological alterations on non-target organisms. This study determined the angiogenesis activity in the chorioallantoic membrane of urea-exposed duck embryos. It also investigated the weight, morphometries, and liver histopathology to gather more information on urea fertilizer's toxicity. It was observed that urea promoted angiogenesis in the CAM of duck embryos, especially at higher concentrations (P<0.05). Embryos treated with urea resulted in an alteration of the head-beak length (P<0.05). However, weight, crown-rump length, forelimb length, and hind limb length were not affected. The developing liver of urea-treated embryos showed distortion of the central vein shape and had larger sinusoidal spaces. The presence of Kupffer cells and lipid droplets were observed in the treated section. Congestion of blood cells, haemorrhage, and necrosis of hepatocytes were also observed in the tissue suggesting the extent of damage caused by the fertilizer. The findings of this study showed multiple developmental effects of urea on duck embryos. Further investigations are needed to shed more light on the toxicity of urea fertilizer on vertebrates.

Lead (Pb) poisoning has caused significant mortality in waterfowl populations worldwide. In spite of having been banned since 2003, prevalence of Pb shot ingestion in mallards (Anas platyrhynchos) from the Ebro delta was still 15.5% in 2011–12. We collected mallard eggs from this area to study the effects of maternally transferred Pb on eggshell properties and on immune response and oxidative balance of ducklings. Eggshell Pb levels were positively correlated with Pb levels in the blood of ducklings. Ducklings with blood Pb levels above 180 ng mL−1 showed reduced body mass and died during the first week post hatching. Blood Pb levels positively correlated with humoral immune response, endogenous antioxidants and oxidative stress biomarkers, and negatively correlated with cellular immune response. Pb shot ingestion in birds can result in maternal transfer to the offspring that can affect their developing immune system and reduce their survival in early life stages.

In this paper we present the studies conducted on blood samples taken from Mallards (Anas platyrhynchos). Birds were captured for ringing purposes (n = 43) in two small and two big towns (including highly urbanized areas). For comparison samples of blood from birds shot on fish ponds were used (n = 26). Based on the body mass all sampled individuals can be assessed as being in good condition. Levels of cadmium (Cd), chromium (Cr), copper (Cu), iron (Fe), nickel (Ni), lead (Pb) and zinc (Zn) in blood samples were measured with AAS. Concentrations of metals did not differ statistically between sexes and made up a following order: Fe > Zn > Cu > Cr ≈ Ni > Pb > Cd. Mallards from towns revealed lower concentrations of Zn and Cu but higher concentration of Fe. There was no difference in exposition to Pb between birds from towns and fish ponds.

The Hudson River, NY, USA is contaminated for over 300 km with polychlorinated biphenyls (PCBs) released from two General Electric (GE) capacitor plants. We collected adult and juvenile mallards (Anas platyrhynchos) from four different areas of the river; an area upstream of the GE plants (n = 38), two areas directly downstream of the GE plants (n = 41, n = 38), and an area more than 100 km downstream in the freshwater tidal river (n = 20). Collections occurred during July and August (2008) when ducks were flightless to ensure ducks were “resident” and exposures were local. Fat and muscle tissue were analyzed for PCBs. PCBs were detected in all samples, and mallards below the GE plant sites on the Hudson River had orders of magnitude higher concentrations of PCBs than those above the plants. Juvenile mallards from areas directly downstream of the GE plant sites tended to have higher PCB concentrations in fat than adults. The patterns of PCB congeners and homolog groups varied across the study areas, with areas directly downstream of the GE plants dominated by tetra-chloro biphenyls whereas samples from upstream and the freshwater tidal river tended towards higher chlorinated congeners. Congener patterns between male and female and juvenile and adult mallards were generally similar within study areas, with the exception of one area downstream of the GE plants where adult birds exhibited different patterns than juveniles. Evidence of PCBs from the GE plant sites was detected in the tidal Hudson River, more than 100 km downstream of the plant sites. More than 90% of the ducks collected in areas downstream of the GE plants but above the tidally influenced river exceed the USFDA tolerance level for PCBs in poultry, which should be a concern for consumers of waterfowl taken in proximity to the upper Hudson River.

Aquatic pollution events can be detrimental to the survival of wildlife, particularly birds. To decontaminate affected birds, large quantities of fresh water are required. A recent study using seabird feathers, demonstrated that seawater wash/rinse can effectively remove oil from feathers. However to determine whether seawater was effective for live birds, we used 36 mallard ducks to replicate the oiled feather wash/rinse study. We investigated the time and volume of water used, bird water-proofing scores after daily swims and a barbule amalgamation index (BAI), for feathers collected at stages throughout the process. Results indicate that for oiled mallard ducks, the use of seawater for decontamination wash/rinse was effective. Seawater wash however, took longer and used a greater quantity of water. Time to birds being waterproof, was not significantly different between groups. The use of seawater has worldwide application for oiled wildlife response activities particularly in areas where freshwater supplies are limited.

Relatively little attention has been paid to the occurrence of anthropogenic debris found in coastal species, especially waterfowl. We examined the incidence of ingested plastic and metal in three waterfowl species wintering in Atlantic Canada: American black ducks (Anas rubripes) and mallards (A. platyrhynchos), two species that use marine and freshwater coastal habitats for foraging in the winter, and common eider (Somateria mollissima), a coastal marine species that feeds on intertidal and subtidal benthic organisms. Plastic was found in the stomachs of 46.1% (6/13) of mallards and 6.9% (6/87) of black ducks, the first report of ingested anthropogenic debris in these species, while 2.1% (1/48) of eider stomachs contained plastic. Metal was found in the stomachs of 30.8% (4/13) of mallards, 2.3% (2/87) of black ducks, and in 2.1% (1/48) of eiders. Our results indicate that species using coastal marine and freshwater environments are exposed to and ingest anthropogenic debris.

Toll-like receptors (TLRs), type I transmembrane pattern recognition receptors (PRRs), are composed of the extracellular domain that is implicated in the recognition of microbial products and initiates the innate and adaptive immune response. Previous reports on TLRs in birds showed significant levels of inter- and intraspecific genetic variation. Little is known about the structure and function of the avian immune system, especially waterfowl species. This work aimed to identify and clone Anas platyrhynchos (mallard duck) TLR-3 (dTLR-3) and its expression level following challenge with velogenic Newcastle disease virus (NDV) as a model for waterfowl species. The mallard duck TLR-3 full-length cDNA sequence had been cloned, which consisted of 2457 nucleotides. The translated amino acid sequence showed identity degree as 97% with Muscovy duck, 95% with geese, 89% with helmeted guineafowls, 88% with the chickens TLR-3 gene, 82% with turkey TLR-3, and 79% with zebra finch, while it showed 54% with human one; the analysis data suggested that the new sequence is probably homologous to vertebrates’ TLR-3. The predicted protein encoded by the duck dTLR-3 mRNA sequence is composed of 819 amino acids. Analysis of the deduced amino acid sequence indicated that dTLR-3 has typical structural features and contains the main components of proteins in the TLR family. The dTLR-3 expressed in almost all examined tissues of mallard duck following quantitative real-time polymerase chain reaction (qPCR) analysis and using B-actin as a housekeeping gene. To check the functionality of the receptor and its role in viral infection, we evaluate the expression level in different tissues and its changes following NDV infection. The results showed significant (P < 0.05) upregulated in the brain at 24 h (1.84-fold), reached a peak at 48 h (4.82-fold), and recovered to normal levels at 72 h post-infection. These results indicate a complete and functional dTLR-3 that is orthologous to other vertebrate receptors with its potential role in early response against viral infection in mallard duck species.

The transfer and fate of methylmercury (MeHg) in typical components, such as sediment, sediment-inhabiting animals, pelagic fish, and three large waterfowls, namely, red-crowned crane (Grus japonensis), mallard (Anas platyrhynchos), and oriental stork (Ciconia boyciana), of the ecosystem in China’s Zhalong Wetland were examined using equivalence-based mass balance model. The biomagnification degree of MeHg increased on the species at the high trophic level of the system. Hence, elevated MeHg concentration (3.2 μg g⁻¹, dry weight) was detected in the endangered G. japonensis. The accumulation of the organometal generally followed the decreasing order of oriental stork (carnivore) > mallard (omnivore) > red-crowned crane (omnivore). The predicted results of MeHg at each node of the food web were generally in accordance with the measured values (F = 0.09, P = 0.78), implying that the model is suitable for the prediction of MeHg fate in the inland aquatic system. According to the model, the respiration for the species at low trophic strata was the key input source of MeHg, but ingestion played an important role for MeHg intake in the species at the high trophic position in the food web. Metabolism was a crucial pathway of MeHg loss for the top predators in the ecosystem.