The application of plant protection products (PPPs) may have delayed and long-term non-intentional impacts on aquatic invertebrates inhabiting agricultural landscapes. Such effects may induce population responses based on developmental and transgenerational plasticity, selection of genetic resistance, as well as increased extirpation risks associated with random genetic drift. While the current knowledge on such effects of PPPs is still scarce in non-target aquatic invertebrate species, evidences are accumulating that support the need for consideration of evolutionary components of the population response to PPPs in standard procedures of risk assessment. This mini-review, as part of a contribution to the collective scientific assessment on PPP impacts on biodiversity and ecosystem services performed in the period 2020–2022, presents a brief survey of the current results published on the subject, mainly in freshwater crustaceans, and proposes some research avenues and strategies that we feel relevant to fill this gap.

International audience | The application of plant protection products (PPPs) may have delayed and long-term non-intentional impacts on aquatic invertebrates inhabiting agricultural landscapes. Such effects may induce population responses based on developmental and transgenerational plasticity, selection of genetic resistance, as well as increased extirpation risks associated with random genetic drift. While the current knowledge on such effects of PPPs is still scarce in non-target aquatic invertebrate species, evidences are accumulating that support the need for consideration of evolutionary components of the population response to PPPs in standard procedures of risk assessment. This mini-review, as part of a contribution to the collective scientific assessment on PPP impacts on biodiversity and ecosystem services performed in the period 2020-2022, presents a brief survey of the current results published on the subject, mainly in freshwater crustaceans, and proposes some research avenues and strategies that we feel relevant to fill this gap.

Freshwater ecosystems are the main source of water for sustaining life on earth, and the biodiversity they support is the main source of valuable goods and services for human populations. Despite growing recognition of the impairment of freshwater ecosystems by micropollutant contamination, different conceptual and methodological considerations can newly be addressed to improve our understanding of the ecological impact into these ecosystems. Here, we originally combined in situ ecotoxicology and community ecology concepts to unveil the mechanisms structuring macroinvertebrate communities along a regional contamination gradient. The novelty of our study lies in the use of an innovative biomonitoring approach (measurement of metal contents in caged crustaceans) allowing to quantify and compare on a regional scale the levels of bioavailable metal contamination to which stream communities are exposed. We were hence able to identify 23 streams presenting a significant gradient of bioavailable metal contamination within the same catchment area in the South West of France, from which we also obtained data on the composition of resident macroinvertebrate communities. Analyses of structural and functional integrity of communities revealed an unexpected decoupling between taxonomic and functional diversity of communities in response to bioavailable metal contamination. We show that despite the negative impact of bioavailable metal contamination exposure on taxonomic diversity (with an average species loss of 17% in contaminated streams), functional diversity is maintained through a process of non-random species replacement by functional redundant species at the regional scale. Such unanticipated findings call for a deeper characterization of metal-tolerant communities’ ability to cope with environmental variability in multi-stressed ecosystems.

Environmental degradation of rivers in agricultural landscapes is typically caused by multiple co-occurring stressors, but how interactions among stressors affect freshwater ecosystems is poorly understood. Therefore, we investigated the sensitivity and specificity of several measures of benthic macroinvertebrate community response to the individual and combined effects of the pesticide sulfoxaflor (SFX), increased sand sedimentation and elevated nutrients using outdoor recirculating mesocosms. Among the single stressor treatments, nutrients had no observable impact and sand only affected one community response measure compared to controls. High SFX levels had the largest effects on benthic macroinvertebrate communities, negatively affecting six of seven macroinvertebrate response measures. Sulfoxaflor had similar adverse effects on biota when in combination with sand and nutrients in the multi-stressor treatment, suggesting that generally SFX has overwhelming and pervasive effects irrespective of the presence of the other stressors. In contrast to SFX, elevated nutrients had no detectable effect on macroinvertebrate communities, likely as a consequence of nutrients being rapidly taken up by bacteria rather than by benthic algae. Elevated sand sedimentation increased the negative effects of SFX on sediment sensitive taxa, but generally had limited biological effects. This was despite the levels of sedimentation in our treatments being at concentrations that have caused large impacts in other studies. This research points to direct and rapid toxic effects of SFX on stream macroinvertebrates, contrasting with effects of the other stressors. This study emphasises that pesticide effects could be misattributed to other common freshwater stressors, potentially focussing restoration actions on a stressor of lesser importance.

This study provided a comprehensive characterization on ingestion of different typologies of microplastics in several fish and invertebrate species from the Adriatic Sea, considered as a preferential area of plastic accumulation in the Mediterranean. Almost 500 organisms were sampled in the three sectors of Northern, Central and Southern Adriatic, testing the hypothesis that area of collection, habitat and feeding strategy might influence the occurrence of plastic particles in biota. In this study, the overall characterization considered separately plastic microparticles (MPs) from textile microfibers (MFs) which also included natural and semi-synthetic ones. Ingestion of MPs was a widespread phenomenon, but their number (typically 1 or 2) did not reveal any significant relationship with biometric values, geographical areas or ecological features of the species. Conversely, the frequency of ingestion, ranging from 13 to 35% of organisms containing MPs, appeared a more reliable index to highlight such differences, revealing higher values in species from Central and Southern basins compared to the Northern one, as well as in benthopelagic compared to benthic or pelagic organisms. Geographical differences also occurred in terms of size and typology of ingested particles, suggesting the importance of local river runoffs and surface currents dynamics. Textile microfibers (MFs) were also abundant in Adriatic food webs occurring in all the analyzed species with average numbers (3–10) and frequencies (40–70%) higher than those reported for MPs; further, an elevated percentage of MFs (>80%) was of natural or semi-synthetic origin.Overall, this study provided general insights toward the harmonization of a common biomonitoring strategy, as in the context of MSFD, including the suggestion of a frequency-based index and of a multi-species approach to increase the ecological relevance of assessment, as well as the comparability between different areas and trophic webs.

Stressors associated with climate change and contaminants, resulting from the activities of humans, are affecting organisms and ecosystems globally. Previous studies suggest that the unique characteristics of polar biota, such as slower metabolisms and growth, and the generally stable conditions in their natural environment, cause higher susceptibility to contamination and climate change than those in temperate and tropical areas. We investigated the effects of increased temperature and decreased salinity on copper toxicity in four subantarctic marine invertebrates using realistic projected conditions under a future climatic change scenario for this region. We hypothesised that these relatively subtle shifts in environmental stressors would impact the sensitivity of cold-adapted species to copper. The four test species were: a copepod Harpacticus sp.; isopod Limnoria stephenseni; flatworm Obrimoposthia ohlini; and bivalve Gaimardia trapesina. These species occupy a range of ecological niches, spanning intertidal and subtidal nearshore zones. We predicted that species would differ in their tolerance to stressors, depending on where they occurred within this ecological gradient. Organisms were exposed to the multiple stressors in a factorial design in laboratory based toxicity tests. Sensitivity estimates for copper (LC50) were calculated using a novel statistical approach which directly assessed the impacts of the multiple stressors. In three of the four species tested, sensitivity to copper was amplified by small increases in temperature (2-4 °C). The effects of salinity were more variable but a decrease of as little as 2 ppt caused a significant effect in one species. This study provides some of the first evidence that high latitude species may be at increased risk from contaminants under projected future climate conditions. This interaction, between contaminants and the abiotic environment, highlights a potential pathway to biodiversity loss under a changing climate.

Hydroxymethylfurfural (HMF) is a plant-based chemical building block that could potentially substitute petroleum-based equivalents, yet ecotoxicological data of this compound is currently limited. In this study, the effects of HMF on the reproduction and survival of Daphnia magna were assessed through validated ecotoxicological tests. The mechanism of toxicity was determined by analysis of transcriptomic responses induced by exposure to different concentrations of HMF using RNA sequencing. HMF exerted toxicity to D. magna with an EC₅₀ for effects on reproduction of 17.2 mg/l. HMF exposure affected molecular pathways including sugar and polysaccharide metabolism, lipid metabolism, general stress metabolism and red blood cell metabolism, although most molecular pathways affected by HMF exposure were dose specific. Hemoglobin genes, however, responded in a sensitive and dose-related manner. No induction of genes involved in the xenobiotic metabolism or oxidative stress metabolism pathway could be observed, which contrasted earlier observations on transcriptional responses of the terrestrial model Folsomia candida exposed to the same compound in a similar dose. We found 4189 orthologue genes between D. magna and F. candida, yet only twenty-one genes of those orthologues were co-regulated in both species. The contrasting transcriptional responses to the same compound exposed at a similar dose between D. magna and F. candida indicates limited overlap in stress responses among soil and aquatic invertebrates. The dose-related expression of hemoglobin provides further support for using hemoglobin expression as a biomarker for general stress responses in daphnids.

Microplastics exposure could be detrimental to marine organisms especially under high concentrations. However, few studies have considered the multiphasic nature of marine invertebrates' life history and investigated the impact of experiencing microplastics during early development on post-metamorphic stages (legacy effect). Many planktonic larvae can feed selectively and it is unclear whether such selectivity could modulate the impact of algal food-sized microplastic. In this two-stage experiment, veligers of Crepidula onyx were first exposed to additions of algae-sized micro-polystyrene (micro-PS) beads at different concentrations, including ones that were comparable their algal diet. These additions were then either halted or continued after settlement. At environmentally relevant concentration (ten 2-μm microplastic beads ml⁻¹), larval and juvenile C. onyx was not affected. At higher concentrations, these micro-PS fed larvae consumed a similar amount of algae compared to those in control but grew relatively slower than those in the control suggesting that ingestion and/or removal of microplastic was/were energetically costly. These larvae also settled earlier at a smaller size compared to the control, which could negatively affect post-settlement success. Juvenile C. onyx receiving continuous micro-PS addition had slower growth rates. Individuals only exposed to micro-PS during their larval stage continued to have slower growth rates than those in the control even if micro-PS had been absent in their surroundings for 65 days highlighting a legacy effect of microplastic exposure.

In spite of being a widespread activity causing the salinization of rivers worldwide, the impact of potash mining on river ecosystems is poorly understood. Here we used a mesocosm approach to test the effects of a salt effluent coming from a potash mine on algal and aquatic invertebrate communities at different concentrations and release modes (i.e. press versus pulse releases). Algal biomass was higher in salt treatments than in control (i.e. river water), with an increase in salt-tolerant diatom species. Salt addition had an effect on invertebrate community composition that was mainly related with changes in the abundance of certain taxa. Short (i.e. 48 h long) salt pulses had no significant effect on the algal and invertebrate communities. The biotic indices showed a weak response to treatment, with only the treatment with the highest salt concentration causing a consistent (i.e. according to all indices) reduction in the ecological quality of the streams and only by the end of the study. Overall, the treatment's effects were time-dependent, being more clear by the end of the study. Our results suggest that potash mining has the potential to significantly alter biological communities of surrounding rivers and streams, and that specific biotic indices to detect salt pollution should be developed.

While environmental risk assessment is typically based on toxicant concentrations in water and/or sediment, awareness is increasing that internal concentrations or body burdens are the key to understand adverse effects in organisms. In order to link environmental micropollutants as causes of observed effects, there is an increasing demand for methods to analyse these chemicals in organisms. Here, a multi-target screening method based on pulverised liquid extraction (PuLE) and a modified QuEChERS approach with an additional hexane phase was developed. It is capable to extract and quantify organic micropollutants of diverse chemical classes in freshwater invertebrates. The method was tested on gammarids from the Danube River (within the Joint Danube Survey 3) and target compounds were analysed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Furthermore, a non-target screening using high resolution-tandem mass spectrometry (LC-HRMS/MS) was conducted. A total of 17 pollutants were detected and/or quantified in gammarids at low concentrations. Pesticide concentrations ranged from 0.1 to 6.52 ng g−1 (wet weight), those of wastewater-derived pollutants from 0.1 to 2.83 ng g−1 (wet weight). The presence of wastewater-derived pollutants was prominent at all spots sampled. Using non-target screening, we could successfully identify several chlorinated compounds. These results demonstrate for the first time the presence of pesticides and wastewater-derived pollutants in invertebrates of the Danube River.