To investigate the effects of an allelochemical artemisinin extracted from Artemisia annua (A. annua) on cell growth, death mode, and microcystin-LR (MC-LR) changes of Microcystis aeruginosa (M. aeruginosa), a series of morphological and biochemical characteristics were studied. The results showed that artemisinin could inhibit the growth of M. aeruginosa and reduce the content of phycobiliprotein. Under the allelopathy of artemisinin, algae cells deformed due to swelling, which caused cell membranes to rupture and cell contents to leak. FDA/PI double-staining results showed that 15.10–94.90% of algae cells experienced the death mode of necrosis-like. Moreover, there were 8.35–14.50% of algae cells undergoing programmed cell death, but their caspase-3-like protease activity remained unchanged, which may mean that algae cells were not experiencing caspase-dependent apoptosis under artemisinin stress. Attacked by artemisinin directly, both intracellular and extracellular MC-LR increased sharply with the upregulation of mcyB, mcyD, and mcyH. The upregulation multiple of mcyH suggested that M. aeruginosa could accelerate transportation of algal toxin under adverse conditions of artemisinin. Artemisinin not only can inhibit the growth of M. aeruginosa but it also causes the accelerated release and increase of microcystin-LR. These imply that the application of artemisinin should be reconsidered in practical water bodies.

Artemisinin, an antimalarial compound derivated from the cultivated plant Artemisia annua L., is produced in situ through cultivation of A. annua under different climatic conditions. The bioactive compound artemisinin has been observed to spread to the surroundings as well as to leach to surface- and groundwater. To make better risk assessments of A. annua which is cultivated under varying climatic conditions, the temperature-dependent toxicity of artemisinin toward the green algae Pseudokirchneriella subcapitata and the macrophyte Lemna minor was evaluated at temperatures ranging from 10 to 30 °C. To include a possible effect of temperature on the degradation rate of artemisinin, artemisinin concentrations were measured during the experiment and toxicity was related to the time-weighted averages of exposure concentrations. The toxicity of artemisinin toward the macrophyte L. minor and the algae P. subcapitata increased with increasing growth rates, and we conclude that bioavailability plays a minor role in the observed relation between temperature and toxicity of artemisinin. The obtained results are important for possible future risk assessment of A. annua cultivation.

In the Qinghai-Tibet Plateau, both the large daily temperature difference and soil salinization make plants susceptible to abiotic stresses such as freeze-thaw and salinity. Meanwhile, crops in this area can be affected by artemisinin, an antimalarial secondary metabolite produced in Artemisia. Under freeze-thaw and salinity stresses, artemisinin was induced as an allelopathy stress factor to explore the physiological response of highland barley, including the relative electrical conductivity (RC), soluble protein (SP) content, malondialdehyde (MDA) content, antioxidant enzyme activity, and water use efficiency (WUE). Compared with the control group, the contents of RC and MDA in seedling leaves under stress were significantly increased by 24.74–402.37% and 20.18–77.95%, indicating that cell membrane permeability was greatly damaged, and WUE was significantly decreased by 15.77–238.59%. The activity of enzymes increased under single stress and decreased under combined stress. Salinity, artemisinin, and freeze-thaw stress show a synergistic relationship; that is, compound stresses were more serious than single stress. In summary, the results of this study revealed the physiological and ecological responses of barley seedlings under different habitat stresses and the interactions among different stress factors.

The present study is aimed to elucidate the effects of concomitant application of irradiated carrageenan (IC) oligomers and salicylic acid (SA) on Artemisia annua L. varieties, viz. “CIM-Arogya” (tolerant) and “Jeevan Raksha” (sensitive) exposed to arsenic (As) stress. Artemisia annua has been known for its sesqui-terpene molecule artemisinin, which is useful in curing malaria. The two compounds, IC and SA, have been established as effective plant growth-promoting molecules for several agricultural and horticultural crops. To test the stress tolerance providing efficacy of IC and SA, the characterization of various physiological and biochemical parameters, growth as well as yield attributes was done in the present experiment. A. annua plants were given various treatments viz. (i) Control (0) (ii) 45 mg As kg⁻¹ of soil (iii) 80 mg L⁻¹ IC+45 mg As kg⁻¹ of soil (iv) 10⁻⁶ M SA+45 mg As kg⁻¹ of soil (vi) 45 mg As kg⁻¹ soil+80 mg L⁻¹ IC+10⁻⁶ M SA. Plants of A. annua suffered from prominent injuries due to oxidative stress generated by As. At 90 and 120 days after planting (DAP), As toxicity was manifested in reduction of most of the studied growth parameters. However, antioxidant activities such as catalase (CAT), peroxidase (POX), superoxide dismutase (SOD), and ascorbate peroxidase (APX) were enhanced in As-stressed conditions and their activities were further enhanced in IC+SA-treated plants. Application of As significantly produced the highest artemisinin content and yield in “CIM-Arogya” over “Jeevan Raksha.” Noticeably, the selected plant growth regulators (PGRs) (IC and SA) applied individually through foliage were found efficient, though, the concomitant effect of PGRs was much pronounced compared to their alone application in countering the toxicity of As. The interactive action of PGRs escalated the overall production (yield) of artemisinin (58.7% and 53.8%) in tolerant and sensitive varieties of A. annua in the presence of soil As. Conclusively, the concomitant application of IC and SA proved much effective and successful over their individual use in exploring the overall development of A. annua subjected to As stress.

Artemisinin sustained-release microspheres (ASMs) with long-term inhibition effects (> 40 days) on harmful freshwater bloom-forming cyanobacteria have been found in previous studies, but the inhibition mechanism is not completely clear. In the present study, we examined the growth effect of ASMs on Microcystis aeruginosa (M. aeruginosa) cells at different physiological stages. Growth experiments indicated that M. aeruginosa of different initial densities could be inhibited immediately and chlorophyll-a content both showed significant decreases following exposure of cyanobacteria to optimal dosage of ASMs for 20 days. The algicidal mechanism of ASMs was tested through a suite of physiological parameters (membrane permeability, antioxidant enzymes activity, and lipid peroxidation). The rise of cell membrane permeability indices (intracellular protein, nucleic acid contents, and conductivity) showed that the cellular membrane structure of M. aeruginosa was attacked by ASMs directly causing the leakage of cytoplasm. Antioxidant enzyme activity was a sensitive indicator of the impacts of ASMs which showed a significant downtrend after a few days. ASMs caused a great increase in •O₂⁻ and malondialdehyde (MDA) level of the algal cells which indicated the increase in lipid peroxidation of M. aeruginosa. Irreversible membrane damage induced by ASMs via the oxidation of ROS may be an important factor responsible for the algicidal mechanism of ASMs on M. aeruginosa cells. The application of ASMs might provide a new direction to control M. aeruginosa, especially before the exponential phase according to the optimal economy and inhibition effect. Graphical abstract

To safely and effectively apply artemisinin sustained-release granules to control and prevent algal water-blooms, the effects of artemisinin and its sustained-release granules on freshwater alga (Scenedesmus obliquus (S. obliquus) and Microcystis aeruginosa (M. aeruginosa)), as well as the production and release of microcystins (MCs) were studied. The results showed that artemisinin sustained-release granules inhibited the growth of M. aeruginosa (above 95 % IR) and S. obliquus (about 90 % IR), with M. aeruginosa more sensitive. The artemisinin sustained-release granules had a longer inhibition effect on growth of pure algae and algal coexistence than direct artemisinin dosing. The artemisinin sustained-release granules could decrease the production and release of algal toxins due to the continued stress of artemisinin released from artemisinin sustained-release granules. There was no increase in the total amount of MC–LR in the algal cell culture medium.

Mosquitoes act as vectors of devastating pathogens and parasites, representing a key threat for millions of humans and animals worldwide. The control of mosquito-borne diseases is facing a number of crucial challenges, including the emergence of artemisinin and chloroquine resistance in Plasmodium parasites, as well as the presence of mosquito vectors resistant to synthetic and microbial pesticides. Therefore, eco-friendly tools are urgently required. Here, a synergic approach relying to nanotechnologies and biological control strategies is proposed. The marine environment is an outstanding reservoir of bioactive natural products, which have many applications against pests, parasites, and pathogens. We proposed a novel method of seaweed-mediated synthesis of silver nanoparticles (AgNP) using the spongeweed Codium tomentosum, acting as a reducing and capping agent. AgNP were characterized by UV–Vis spectroscopy, Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD). In mosquitocidal assays, the 50 % lethal concentration (LC₅₀) of C. tomentosum extract against Anopheles stephensi ranged from 255.1 (larva I) to 487.1 ppm (pupa). LC₅₀ of C. tomentosum-synthesized AgNP ranged from 18.1 (larva I) to 40.7 ppm (pupa). In laboratory, the predation efficiency of Mesocyclops aspericornis copepods against A. stephensi larvae was 81, 65, 17, and 9 % (I, II, III, and IV instar, respectively). In AgNP contaminated environment, predation was not affected; 83, 66, 19, and 11 % (I, II, III, and IV). The anti-plasmodial activity of C. tomentosum extract and spongeweed-synthesized AgNP was evaluated against CQ-resistant (CQ-r) and CQ-sensitive (CQ-s) strains of Plasmodium falciparum. Fifty percent inhibitory concentration (IC₅₀) of C. tomentosum were 51.34 μg/ml (CQ-s) and 65.17 μg/ml (CQ-r); C. tomentosum-synthesized AgNP achieved IC₅₀ of 72.45 μg/ml (CQ-s) and 76.08 μg/ml (CQ-r). Furthermore, low doses of the AgNP inhibited the growth of Bacillus subtilis, Klebsiella pneumoniae, and Salmonella typhi, using the agar disk diffusion and minimum inhibitory concentration protocol. Overall, C. tomentosum metabolites and spongeweed-synthesized AgNP may be potential candidates to develop novel and effective tools in the fight against Plasmodium parasites and their mosquito vectors. The employ of ultra-low doses of nanomosquitocides in synergy with cyclopoid crustaceans seems a promising green route for effective mosquito control programs.