This study aims to investigate the toxicity of the plant to heavy elements (HMs). For this purpose, the estimated daily intake (EDI) parameters of potentially toxic elements (PTE) per kilogram of body weight, target hazard quotient (THQ) for non-carcinogenic disorders, total hazard index (HI), and bioconcentration factor (BCF) are determined in the plant at different stages of growth. In this study, the reaction of the plant to different electromagnetic waves at different stages of growth (DSG) is also investigated, and the relationship between the THQ values and electromagnetic waves is prepared. The results show that Pb has the highest EDI value (5.97), Pb (74.67) and Cd (9.75) have the highest THQ values, and Cd has the highest BCF value (30.44). Also, the results show that HI values are higher than the threshold in the growth (69.98), flowering (71.38), and fruiting (68.06) stages. Results of BCF indicate Pb, and Cd has absorption rate in Capsicum towards. Contaminated Capsicum plants submitted to electromagnetic waves showed a significant relationship between Pb and the b490, and b560 spectra, Cd and Ni the b450 spectrum, and Zn the b460 spectrum. This finding highlights the salience of employing electromagnetic waves in assessing contamination in plants. Put differently, THQ can be estimated using electromagnetic waves without any need for laboratory studies.

Tebuconazole is a broad-spectrum fungicide extensively used worldwide for the control of many diseases such as powdery mildew and scab in apple, early blight of tomato, anthracnose of chilli, white rot and purple blotch of onion etc. Maximum residue level of this compound has not been worked out on these crops in India; the persistence and dissipation kinetics of tebuconazole on apple, tomato, chilli and onion were studied following three foliar applications of the formulation Folicur 430 SC at a standard dose (X) 322, 268.75, 215 and 215 g a.i./ha and at double dose (2X) 645, 537.5, 430 and 430 g a.i./ha, respectively, to work out the safe waiting periods and half-life period of tebuconazole. Extraction was done using QuEChERS method and cleanup by using dispersive solid-phase method. Tebuconazole residues were estimated on gas chromatograph-mass spectrometry (GC-MS). The recovery of tebuconazole in fortified matrix was above 90% with a limit of quantification (LOQ) at 0.05 mg kg⁻¹. The initial deposits of tebuconazole on apple at two locations under study ranged from 1.986–2.011 mg kg⁻¹at X dose to 3.698–3.843 mg kg⁻¹ at 2X dose. The initial deposits in tomato, chilli and onion were 1.129, 1.760 and 1.169 mg kg⁻¹ at X dose and 2.213, 2.784 and 2.340 mg kg⁻¹, respectively at the 2X dose. Dissipation of the fungicide followed first-order of kinetics and the half life of degradation ranged from 1.30–2.25 days at X dose to 1.40–2.62 days at 2X days on different crops under study. Residues declined below the determination limit (LOQ) of 15 and 20 days after spraying, respectively, at X and 2X dose in apple; 7 and 10 days in tomato; 10 and 15 days in chilli and onion. Waiting periods of 5, 2, 7 and 12 days, respectively, are suggested for apple, tomato, chilli and onion at 2X dose.

The reasons why some cultivars of hot pepper (Capsicum annuum L.) accumulate low levels of Cd are poorly understood. We aimed to compare the characteristics of Cd uptake and translocation in low-Cd and high-Cd hot pepper cultivars by determining the subcellular locations and chemical forms of Cd, and its distribution among different plant organs. We conducted a hydroponic experiment to investigate the subcellular distribution and chemical forms of Cd in roots, stems, and leaves of a low-Cd (Yeshengchaotianjiao, YCT) and a high-Cd cultivar (Jinfuzaohuangjiao, JFZ). The results showed that the concentrations of Cd in almost all subcellular fractions of roots, and in all chemical forms in roots, were higher in YCT than in JFZ. Compared with YCT, JFZ had higher Cd concentrations in almost all subcellular fractions of stems and leaves, and higher Cd concentrations in almost all chemical forms in stems and leaves. Additionally, YCT had significantly higher total Cd accumulation but a lower Cd translocation rate compared with JFZ. In general, the results presented in this study revealed that root-to-shoot Cd translocation via the xylem is the key physiological processes determining the Cd accumulation level in stems and leaves of hot pepper plants. Immobilization of Cd by the cell walls of different organs is important in Cd detoxification and limiting the symplastic movement of Cd.