A native Indian catfish (Heteropneustes fossilis) and an Indian major carp (Cirrhinus cirrhosus) were treated with sodium dodecyl sulfate (SDS) to elucidate its consequences on antioxidant status and sex steroid synthesis. Fishes were exposed to 1/10th of LC₅₀ of SDS for 30 days. Different enzymatic and non-enzymatic antioxidants, major sex steroids, steroidogenic enzymes, gonadosomatic index (GSI), and serum cortisol level were measured in both male and female fishes. Levels of enzymatic and non-enzymatic antioxidants altered significantly in both the fish species. Steroid hormones in both male and female fishes decreased significantly in the SDS-treated group. Profiles of steroidogenic enzymes (hydroxysteroid dehydrogenase; 3β-HSD and 17β-HSD) show significant decrease in the SDS treatment group in both carp and catfish than that of the control group irrespective of sexes. GSI also decreases significantly in the SDS-treated group when compared with the control group in spawning phase of carp and catfish irrespective of sexes. Serum cortisol level was increased significantly after SDS exposure. Higher level of cortisol was noted in the spawning phase than that of pre-spawning phase. Current study proves that SDS could interrupt the normal steroidogenesis process in fish, leading to impaired sex hormone production. Detrimental effect of SDS was noted to be more prominent on female steroidogenesis than that of male fish. A species or season-specific variation in steroidogenesis was also observed which indicates that SDS-induced toxicity depends on the sexual status of the specific fish species.

The effect of waterborne zinc on survival, growth, and feed intake of Indian major carp, Cirrhinus mrigala (Hamilton), advanced fry was studied under laboratory condition. Survival rates of C. mrigala advanced fry (2.71 ± 0.49 g) after 30 days exposure to control (0.01), 0.03, 0.06, 0.10, and 0.15 mg/L zinc using the static renewal method in freshwater at pH 7.3 ± 0.2, temperature 26 ± 2°C, and total hardness 114 ± 16 mg/L as CaCO₃ were 100%. Growth of the fish exposed to 0.10 and 0.15 mg/L of zinc was significantly lower (P < 0.05) than in control (0.01), 0.03, and 0.06 mg/L of zinc after 30 days of exposure. However, there were no significant differences (P > 0.05) in fish growth between 0.03 and 0.06 mg/L zinc concentrations. Feed intake rates were significantly (P < 0.05) reduced in the fish exposed to 0.10 mg/L and higher levels of zinc. The zinc accumulation in the whole body of the fish increased with increasing concentrations of the metal.

The aim of the present study was to describe the accumulation of trace metals in the liver, kidney, gills, muscles, and skin of four edible fish species (Tor putitora, Cirrhinus mrigala, Labeo calbasu, and Channa punctatus) of Rawal Lake Reservoir, Pakistan. The fish samples were collected in the pre-monsoon (May 2008) and post-monsoon (October 2007) seasons and were analyzed for heavy metals by using an atomic absorption spectrometer. Kidney and liver showed relatively high concentrations of heavy metals. The accumulation of metals in the different organs of the fish (skin, muscles, and gills) in post-monsoon was higher than in pre-monsoon. In pre-monsoon, the metals followed the trend Zn > Pb > Fe > Cr > Ni > Mn > Co > Cu > Cd > Li, while in the post-monsoon season, the trend was Fe > Pb > Cr > Ni > Zn > Cu > Co > Mn > Cd > Li. The concentrations of Ni, Cr, and Pb in the muscle of all fish species were higher than the WHO guideline values of heavy metals in fishes for human consumption except in T. putitora. Cu level was nearly equal to the WHO maximum levels in C. mrigala and L. calbasu, while it was lower in T. putitora and C. punctatus. It is strongly advocated that risk assessment studies should be conducted and there is an urgent need for water quality restoration and management of Rawal Lake Reservoir.

The current study demonstrates oxidative damage and associated neurotoxicity following pH stress in two freshwater carp Labeo rohita and Cirrhinus cirrhosus. Carp (n = 6, 3 replicates) were exposed to four different pH (5.5, 6, 7.5, and 8) against control (pH 6.8 ± 0.05) for 7 days. After completion of treatment, levels of enzymatic (superoxide dismutase [SOD], catalase [CAT], glutathione reductase [GRd]) and non-enzymatic antioxidants (malondialdehyde [MDA], glutathione [GSH]), brain neurological parameters (Na⁺-K⁺ATPase, acetylcholinesterase [AcHE], monoamine oxidase [MAO], and nitric oxide [NO]), xanthine oxidase (XO), heat shock proteins (HSP70 and HSP90), and transcription factor NFkB were measured in carp brain. Variation in the pH caused a significant alteration in the glutathione system (glutathione and glutathione reductase), SOD-CAT system, and stress marker malondialdehyde (MDA). Xanthine oxidase was also induced significantly after pH exposure. Brain neurological parameters (MAO, NO, AChE, and Na⁺-K⁺ATPase) were significantly reduced at each pH-treated carp group though inhibition was highest at lower acidic pH (5.5). Cirrhinus cirrhosus was more affected than that of Labeo rohita. Molecular chaperon HSP70 expression was induced in all pH-treated groups though such induction was more in acid-stressed fish. HSP90 was found to increase only in acid-stressed carp brain. Expression of NFkB was elevated significantly at each treatment group except for pH 7.5. Finally, both acidic and alkaline pH in the aquatic system was found to disturb oxidative balance in carp brain which ultimately affects the neurological activity in carp. However, acidic environment in the aquatic system was more detrimental than the alkaline system regarding oxidative damage and subsequent neurotoxicity in carp brain.

Concentrations of heavy metals (Cu, Ni, Zn, Cd and Pb) were measured in sediments, water and liver and kidney tissues of three Indian major carps (Labeo rohita, Catla catla and Cirrhinus cirrhosus), belonging to two different weight groups (250 and 500 g), collected from ponds at two different sites (Nalban bheri and Diamond Harbour). The tissues were analysed for the levels of different antioxidant defence systems such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GRd), glutathione S-transferase (GST), glutathione (GSH) and malondialdehyde (MDA). Concentrations of all the metals were significantly higher (P < 0.05) in sediment, water and the tissues from Nalban bheri compared to those in Diamond Harbour. Metal concentrations were the lowest in C. cirrhosus, which increased with an increase in fish weight, and the liver accumulated higher amount of metals than the kidney. Activities of all enzymatic and non-enzymatic antioxidant parameters except GPx and GRd were significantly higher (P < 0.05) in the tissues from Nalban bheri than those in Diamond Harbour. Significant multicollinearity was found in the values of SOD, CAT, GST, GRd, GPx and MDA with Pb, Cu and Ni in all three fish species at Nalban and with Cd in L. rohita and C. catla. Principal component analysis results revealed that stress response in a polluted site was directly regulated by an amalgamation of GSH profile and the levels of MDA in a synchronized manner. The study indicated a tissue-specific and species-specific difference for heavy metal-induced oxidative stress response in fish and a correlation between different heavy metals and individual oxidative stress markers.