The current air pollution by SO₂ due to anthropogenic pressure in Poland was assessed based on sulfur concentrations in pine needles (Pinus sylvestris L.). On 308 monitoring sample plots located in pine stands distributed across Poland, measurements were conducted in mineral soil layers (0–10 cm, 10–40 cm, 40–100 cm) and in the soil organic layer (+5–0 cm). Samples of Scots pine foliage (current-year needles) were then collected, and the sulfur concentration in these needles was determined. Based on these data, a map of the spatial variability of sulfur concentrations in pine needles was drawn. The mean sulfur concentration in the pine needles was 854.8 mg kg⁻¹ in dry mass. Higher SO₂ emissions were noted in regions influenced by industry, such as the Upper Silesia and regions under strong urban pressure. Sulfur concentrations in Scots pine needles were related to the stands' degrees of defoliation. A comparison of the current sulfur concentrations in pine needles from biomonitoring in 2015–2016 with those from previous biomonitoring (in 1983–1985, by Dmuchowski and Bytnerowicz (1995) showed that air quality has improved and SO₂ emissions have decreased.

The application of biochar in remediation and recovery of heavy metals and/or organic contaminants in water and soil is increasing. However, the adverse effect of biochar to aquatic organisms has not received enough attention. In this study, we conducted a study on the biotoxicity of biochar pyrolyzed from pine needle under oxygen-limited conditions. The toxicity of biochar was expressed with the following endpoints: cell growth, chlorophyll-a (Chl-a), reactive oxygen species (ROS), superoxide dismutase (SOD) content of Scenedesmus obliquus (S. obliquus) and the luminescence of Photobacterium phosphoreum (P. phosphoreum). Here, the effect of free radicals (FRs) contained in biochar was stressed. Our results show that the toxicity of biochar is significantly correlated with the concentration of FRs in biochar particles. Meanwhile, we found the FRs-containing biochar could induce the production of acellular ROS (such as ·OH) in water, which would also induce the production of interior cellular ROS in aquatic organisms. Our findings provide a new insight into the mechanism of toxicity aroused by biochar applications and aid in understanding its potential ecological risk.

We evaluated spatial patterns of mercury (Hg) deposition through analysis of foliage and forest floor samples from 45 sites across Adirondack Park, NY. Species-specific differences in foliar Hg were evident with the lowest concentrations found in first-year conifer needles and highest concentrations found in black cherry (Prunus serotina). For foliage and forest floor samples, latitude and longitude were negatively correlated with Hg concentrations, likely because of proximity to emission sources, while elevation was positively correlated with Hg concentrations. Elemental analysis showed moderately strong, positive correlations between Hg and nitrogen concentrations. The spatial pattern of Hg deposition across the Adirondacks is similar to patterns of other contaminants that originate largely from combustion sources such as nitrogen and sulfur. The results of this study suggest foliage can be used to assess spatial patterns of Hg deposition in small regions or areas of varied topography where current Hg deposition models are too coarse to predict deposition accurately.

Biochar plays an important role in the behaviors of organic pollutants in the soil environment. The role of humic acid (HA) and metal cations on the adsorption affinity of polychlorinated biphenyls (PCBs) to the biochars in an aqueous medium and an extracted solution from a PCBs-contaminated soil was studied using batch experiments. Biochars were produced with pine needles and wheat straw at 350 °C and 550 °C under anaerobic condition. The results showed that the biochars had high adsorption affinity for PCBs. Pine needle chars adsorbed less nonplanar PCBs than planar ones due to dispersive interactions and separation. Coexistence of HA and metal cations increased PCBs sorption on the biochars accounted for HA adsorption and cation complexation. The results will aid in a better understanding of biochar sorption mechanism of contaminants in the environment.

Population variation in ecophysiological traits of four co-occurring montane conifers was measured on a large latitudinal gradient to quantitatively assess their potential for response to environmental change. White fir (Abies concolor) had the highest variability, gross photosynthetic rate (Pg), and foliar carbon (C) and nitrogen (N) content. Despite low water use efficiency (WUE), stomatal conductance (gs) of fir was the most responsive to unfavorable environmental conditions. Pinus lambertiana exhibited the least variability in Pg and WUE, and is likely to be the most vulnerable to environmental changes. Pinus ponderosa had an intermediate level of variability, and high needle growth at its higher elevational limits. Pinus Jeffreyi also had intermediate variability, but high needle growth at its southern latitudinal and lower elevational limits. The attributes used to assess tree vigor were effective in predicting population vulnerability to abiotic (drought) and biotic (herbivore) stresses. Variability in ecophysiological attributes of western U.S. conifers suggests relative capacity of species and populations to respond to environmental change.

Recent studies have demonstrated that natural abundance 15N can be a useful tool for assessing nitrogen saturation, because as nitrification and nitrate loss increase, δ15N of foliage and soil also increases. We measured foliar δ15N at 11 high-elevation spruce-fir stands along an N deposition gradient in 1987-1988 and at seven paired northern hardwood and spruce-fir stands in 1999. In 1999, foliar δ15N increased from -5.2 to -0.7[per thousand] with increasing N deposition from Maine to NY. Foliar δ15N decreased between 1987-1988 and 1999, while foliar %N increased and foliar C:N decreased at most sites. Foliar δ15N was strongly correlated with N deposition, and was also positively correlated with net nitrification potential and negatively correlated with soil C:N ratio. Although the increase in foliar %N is consistent with a progression towards N saturation, other results of this study suggest that, in 1999, these stands were further from N saturation than in 1987-1988. Foliar δ15N increased with increasing N deposition from Maine to NY, but decreased between 1987-1988 and 1999

Research is restricted regarding impacts of biomass burning (BB) on fine aerosol (PM₂.₅), due mainly to lack of specific BB tracers. This study aims to characterize the variability, distributions, and contributions of BB and fungal spores as sources of PM₂.₅ using a multiple organic tracer approach. PM₂.₅ samples were collected at four representative sites in Yangtze River Delta (YRD), China every 6 days for one year. In the laboratory, samples were analyzed for three anhydrides (levoglucosan, mannosan, and galactosan), two sugar alcohols (arabitol and mannitol), water-soluble inorganic ions, and elemental/organic carbon (EC/OC). Levoglucosan was the most abundant BB tracer (mean concentration = 81 ng/m³), and fungal spore tracers arabitol and mannitol had similar abundances (5.6 and 5.7 ng/m³, respectively). Anhydrides and sugar alcohols had high within-group correlations, indicating their respective common sources. Concentrations of tracers displayed large temporal variations but small spatial variations, suggesting strong seasonality in BB and fungal spore sources. BB sources were burning of grass, pine needles, hardwood and crop straw, which were originated from transboundary/cross-region transport and local fire spots. PCA analyses revealed that the common sources of fine aerosols in YRD were secondary inorganic aerosols, soil dust, BB and fungal spores.

Twenty-six polycyclic aromatic hydrocarbons (PAHs) and four synthetic musk compounds (SMCs) accumulated by Masson pine needles from different areas of Shanghai were investigated in the present study. Concentrations of Σ26PAHs (sum of 26 PAHs) ranged from 234 × 10−3 to 5370 × 10−3 mg kg−1. Levels of Σ26PAHs in different sampling areas followed the order: urban areas (Puxi and Pudong) > suburbs > Chongming. Total concentrations of 16 USEPA priority PAHs ranged from 225 × 10−3 to 5180 × 10−3 mg kg−1, ranking at a relatively high level compared to other regions around the world. Factor analysis and multi-linear regression model has identified six sources of PAHs with relative contributions of 15.1% for F1 (vehicle emissions), 47.8% for F2 (natural gas and biomass combustion), 7.8% for F3 (oil), 10.6% for F4 (coal combustion), 15.7% for F5 (“anthracene” source) and 3.0% for F6 (coke tar). Total concentrations of 4 SMCs varied between 0.071 × 10−3 and 2.72 × 10−3 mg kg−1 in pine needles from Shanghai. SMCs with the highest detected frequency were Galaxolide and musk xylene, followed by musk ketone and Tonalide. The highest level of SMCs was found near industrial park and daily chemical plant. The results obtained from this study may have important reference value for local government in the control of atmospheric organic pollution.

Tree canopies play a key role in the cycling of polycyclic aromatic hydrocarbons (PAHs) in terrestrial ecosystems, as leaves can capture PAHs from the air. In this study, accumulation of PAHs was compared in an evergreen species, P. pinaster, and in a deciduous species, Q. robur, in relation to some physio-morphological characteristics. For this purpose, pine needles and oak leaves collected from different sites across Galicia (NW Spain) were analysed to determine PAH contents, specific leaf area, stomatal density and conductance.Leaves and needles contained similar total amounts of PAHs. The major contribution of particle-bound PAHs in oak (the concentrations of 4- and 5-ring PAHs were two times higher, and those of 6-ring PAHs five times higher in oak than in pine) may be related to the higher specific leaf area (13 and 4 cm2 g−1 dry mass in respectively oak and pine). However, the major contribution of vapor-phase PAHs in pines may be affected by the stomatal conductance (two times higher in pine than in oak). Moreover, an increase in the diameter at breast height of trees led to an increase in accumulation of PAHs, with pine capturing higher amounts of low and medium molecular weight PAHs. The study findings underline the potential role of trees in improving air quality, taking into account the canopy biomass and life cycle.

To better understand interaction mechanisms of pine needles with persistent organic pollutants, single-solute and bi-solute sorption of phenanthrene and pyrene onto isolated cuticular fractions of pine needle were investigated. The structures of cuticular fractions were characterized by elemental analysis, Fourier transform infrared spectroscopy and solid-state 13C NMR. Polymeric lipids (cutin and cutan) exhibited notably higher sorption capabilities than the soluble lipids (waxes), while cellulose showed little affinity with sorbates. With the coexistence of the amorphous cellulose, the sorption of cutan (aromatic core) was completely inhibited, so the cutin components (nonpolar aliphatic moieties) dominated the sorption of bulk needle cuticle. By the consumption of the amorphous cellulose under acid hydrolysis, sorption capacities of the de-sugared fractions were dramatically enhanced, which controlled by the exposed aromatic cores and the aliphatic moieties. Furthermore, the de-sugared fractions demonstrated nonlinear and competitive sorption due to the specific interaction between aromatic cores and polycyclic aromatic hydrocarbon.