Due to worldwide production, sales and application, neonicotinoids dominate the global use of insecticides. While, neonicotinoids are considered as pinpoint neurotoxicants that impair cholinergic neurotransmission in pest insects, the sublethal effects on nontarget organisms and other neurotransmitters remain poorly understood. Thus, we investigated long-term neurological outcomes in the decomposer nematode Caenorhabditis elegans. In the adult roundworm the neonicotinoid thiacloprid impaired serotonergic and dopaminergic neuromuscular behaviors, while respective exposures to thiamethoxam showed no effects. Thiacloprid caused a concentration-dependent delay of the transition between swimming and crawling locomotion that is controlled by dopaminergic and serotonergic neurotransmission. Age-resolved analyses revealed that impairment of locomotion occurred in young as well as middle-aged worms. Treatment with exogenous serotonin rescued thiacloprid-induced swimming deficits in young worms, whereas additional exposure with silica nanoparticles enhanced the reduction of swimming behavior. Delay of forward locomotion was partly caused by a new paralysis pattern that identified thiacloprid as an agent promoting a specific rigidity of posterior body wall muscle cells and peripheral neuropathy in the nematode (lowest-observed-effect-level 10 ng/ml). On the molecular level exposure with thiacloprid accelerated protein aggregation in body wall muscle cells of polyglutamine disease reporter worms indicating proteotoxic stress. The results from the soil nematode Caenorhabditis elegans show that assessment of neurotoxicity by neonicotinoids requires acknowledgment and deeper research into dopaminergic and serotonergic neurochemistry of nontarget organisms. Likewise, it has to be considered more that different neonicotinoids may promote diverse neural end points.

Due to increased use of agrochemicals and growing concerns about ecotoxicology, the development of new insecticides, moving away from those with neurotoxic and broad spectrum effects towards insecticides that are safer for the environment and nontarget beneficial species, has been a research priority. Novaluron stands out among these newer insecticides, is an insect growth regulator that is used for the control of insect pests in crops grown close to mulberry plantations. Mulberry serves as food for the silkworm Bombyx mori, which is a nontarget insect of great economic importance to silk production. We investigated the lethal and sublethal effects of Novaluron on the development of B. mori. Larvae were segregated into experimental groups: the control groups (CGs) and the treatment groups (TGs), which were treated with the Novaluron concentration of 0.15 mL/L. Following exposure, we analyzed: larval mortality, changes in the insect life cicle and cytotoxic effects on the midgut cells. This is the first report about the Novaluron’s effects on B.mori. We detected rupture in the integument, complete cessation of feeding, late development, incomplete ecdysis and production of defective cocoons. After 240 h of exposure, there was 100% mortality in TG larvae exposed in the 3rd instar and 20% mortality from larvae exposed in the 5th instar. Cytotoxic effects was observed, such as dilation of cells, emission of cytoplasmic protrusions, extreme rarefaction of the cytoplasm and nuclei, dilation of the endoplasmic reticulum in addition to changes in mitochondria, the presence of large digestive vacuoles and intercellular spaces and the presence of active caspase. Novaluron exposure impairs the midgut and may affect the physiological functions of this organ. Novaluron additionally compromises several phases of insect development, indicating the importance of toxicology studies that utilize different life stages of nontarget species to evaluate the safe use of insecticides.

Methylmercury accumulated at the top of aquatic food chains constitutes a toxicological risk to humans and other top predators. Biomagnification of methylmercury takes place among vertebrates at the higher trophic levels, but this process is less elucidated in benthic invertebrates at the lower trophic levels. Therefore, we investigated the accumulation from food and elimination of methylmercury and inorganic mercury in the benthic sea star Asterias rubens (L.) – a representative of trophic level ~3 - in laboratory experiments. Sea stars fed over 49 days with contaminated mussels (Mytilus edulis) accumulate methylmercury and inorganic mercury to the highest concentrations in the digestive glands, the pyloric caeca, less in stomach, gonad, tube feet, aboral body wall and not to detectable levels in the coelomic fluid. Concerning whole body contents, steady states were reached for both methylmercury and inorganic mercury during the 7-week feeding period and the sea stars reached approximately ½ and ¼ of the concentrations in the mussel food for the two mercury forms, respectively. Half-lives for the elimination of the two mercury forms varied between 45 and 173 days in a 140-d elimination period following the feeding period; inorganic mercury was eliminated faster than methylmercury. Examination of total mercury concentrations in field-collected sea stars confirmed this lack of trophic magnification in relation to the major food items, soft parts of molluscs. We suggest that mercury is not trophically magnified in sea stars 1) because they eliminate methylmercury faster than larger fish and decapod crustaceans and 2) maybe more importantly, because inorganic mercury with its faster elimination constitutes a larger fraction of the total mercury in the food at the lower trophic levels - as opposed to methylmercury which dominates at the higher trophic levels.

With the constant quest for new sources of superfoods to supplement the largely nutrient deficient diet of the modern society, sea cucumbers are gaining increasing popularity. Three species of sea cucumbers, Cucumaria frondosa, Apostichopus californicus and Apostichopusjaponicus were collected from three geographical regions, Atlantic and Pacific coast of Canada and Yellow sea/ East China sea in China, respectively. These organisms were sectioned into parts (body wall, tentacles, internal organ, skin and muscle) and analysed for total arsenic (As) by inductively coupled plasma mass spectrometry (ICP-MS) and As species by high-performance liquid chromatography (HPLC) coupled to ICP-MS. Normal and reversed sequential extractions were optimised to address As distribution between lipids (polar and non-polar) and water-extractable fractions. Two extraction methods for water-extractable As were compared in terms of the number and the amount of extracted species. The results revealed that total As concentration and As species distribution varies significantly between sea cucumbers species. Total As in studied body parts ranged between 2.8 ± 0.52 and 7.9 ± 1.2 mg kg⁻¹, with an exception of the muscle tissue of A. californicus, where it reached to 36 ± 3.5 mg kg⁻¹. Arsenobetaine (AsB) was the most abundant As species in A. californicus and A.japonicus, however, inorganic As represented over 70% of total recovered As in the body parts of C. frondosa. Arsenosugars-328 and 482 were found in all studied body parts whereas arsenosugar-408 was only found in the skin of A. californicus. This is the first time that such a variation in As species distribution between sea cucumber species has been shown.

The metal detoxification mechanism is essential for the accumulation activity of some polychaetes. In order to investigate the detoxification function of metalloprotein II in polychaete Perinereis aibuhitensis, a recombinant fusion protein of MP II was successfully expressed in vitro and an anti-MP II polyclonal antibody was got. The tissue distribution of MP II in P. aibuhitensis and the protein expression under Cd, Pb and Zn exposure were detected by ELISA with this antibody. The results showed that MP II was higher in the intestine of P. aibuhitensis, followed by the body wall and parapodium. Cd, Zn and Pb exposure can induced MP II expression, but the change trend of MP II under various heavy metal exposure was different. The amount of MP II induced in P. aibuhitensis increased with the rises of Cd concentration, but there is no significant relationship between Zn and Pb concentration and MP II level.

The concentrations of some heavy metals (Cd, Cu, Zn andPb) were investigated in the sediments and the two species of sea cucumber (Holothuria leucospilota and Holothuria scabra) from northern part of Qeshm Island, Persian Gulf. The levels of Cu, Zn and Pb in sediment varied significantly among sampling sites (p<0.05). The highest levels of Zn and Pb in H. leucospilota were recorded in body wall whereas the highest contents of Zn and Pb in H. scabra were measured in respiratory tree organ, respectively. Cu and Cd were the most abundant elements in gonads of H. leucospilota while highest levels of Cd and Cu in H. scabra were measured in the gonad and derm, respectively. Cu and Zn concentrations were below permissible limits for human consumption while Cd and Pb were above permissible limits for human consumption.

The concentrations of eight heavy metals (As, Cd, Cr, Cu, Mn, Ni, Pb and Zn) were measured in the sediment, the isolated microplastics from the sediment and the body wall of sea cucumbers from farms in China. Accordingly, the heavy metal concentrations in the sediment were below the class I upper limit of Chinese sediment quality guidelines. Among heavy metals, the median concentrations of Cd and As were higher in the body wall than in the corresponding sediment. Additionally, the median concentrations of Cd, Pb, and Zn were higher on the microplastics than in the corresponding sediment. Furthermore, there was no significant correlation among heavy metals in sediment, sea cucumber and microplastics. This study contributes to the understanding of the heavy metal accumulation in the sediment, the microplastics and the body wall of the sea cucumber.

Phenanthrene (PHE) contamination not only changes the quality of soil environment but also threatens to the soil organisms. There is lack of focus on the eco-toxicity potential of this contaminant in real soil in the current investigation. Here, we assessed the toxic effects of PHE on earthworms (Eisenia fetida) in natural soil matrix. PHE exhibited a relatively high toxicity to E. fetida in natural soil, with the LC₅₀ determined to be 56.68 mg kg⁻¹ after a 14-day exposure. Excessive ROS induced by PHE, leading to oxidative damage to biomacromolecules in E. fetida, including lipid peroxidation, protein carbonylation, and DNA damage. The antioxidant defense system (total antioxidant capacity, glutathione S-transferase, peroxidase, catalase, carboxylesterase, and superoxide dismutase) in E. fetida responded quickly to scavenge excess ROS and free radicals. Exposure to PHE resulted in earthworm avoidance responses (2.5 mg kg⁻¹) and habitat function loss (10 mg kg⁻¹). Histological observations indicated that the intestine, body wall, and seminal vesicle in E. fetida were severely damaged after exposure to high-dose PHE. Moreover, earthworm growth (weight change) and reproduction (cocoon production and the number of juvenile) were also inhibited after exposure to this pollutant. Furthermore, the integrated toxicity of PHE toward E. fetida at different doses and exposure times was assessed by the integrated biomarker response (IBR), which confirmed that PHE is more toxic to earthworms in the high-dose and long-term exposure groups. Our results showed that PHE exposure induced oxidative stress, disturbed antioxidant defense system, and caused oxidative damage in E. fetida. These effects can trigger behavior changes and damage histological structure, finally cause growth inhibition, genotoxicity, and reproductive toxicity in earthworms. The strength of this study is the comprehensive toxicity evaluation of PHE to earthworms and highlights the need to investigate the eco-toxicity potential of exogenous environmental pollutants in a real soil environment.

The polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs) are persistent environmental pollutants. Recently, there have been an increasing demand to assess different biomarkers as early alarming indicators of environmental pollution. This study is the first to investigate the effects of PCDD/Fs on acetylcholinesterase (AChE) activity and histopathology of the body wall (epidermis, circular, and longitudinal muscles) of earthworm Eisenia andrei (Oligochaeta, Lumbricidae) using acute filter paper toxicity test. It is also exploring the selected biomarkers as a potential tool for evaluating soil quality. Earthworms were exposed to 0, 0.5, 1.0, and 1.5 ng/cm² PCDD/Fs. The treated worms expressed progressive abnormal morphological signs with incrementing doses of dioxins, such as coiling, curling, and body swelling. At the highest dose, some worms demonstrated skin discoloration, loss of body segmentation, and body part detachment. The AChE activity was significantly decreased (p < 0.001) in all treated animals compared to control. The mixture induced circular muscle hyperplasia at 0.5 ng/cm². However, the mixture at 1.5 ng/cm² caused epidermal atrophy with cell pyknosis and necrosis in all layers of the body wall. Image analysis revealed significant reduction in the thickness of the epidermis (p < 0.001) at all doses with relative to control. Herein, we report that 48 h of acute exposure of E. andrei to dioxins/furans induced morphological changes, reduced the activity of AChE, and induced histopathological alterations. The outcomes can be utilized as endpoints that could be added to earthworm’s standardized short tests for ecotoxicity studies.

This study used a formaldehyde detector tube with a gas-piston hand pump to assess ceiling levels of student breathing zone and gross laboratory environment across the 2018 academic year. The room dimension was 28.6 × 55.48 × 5.5 m. It contained 90 cadavers, each placed on a hinged cover table. We measured before and during nine body region dissections. There was a significant difference (p < 0.01) between student exposure and laboratory environment levels. The highest level was student exposure during body wall dissection (2.7 ppm), the first laboratory; students may accidentally enter body cavities. The latter two were in abdominal (1.85 ppm) and lower limb dissections (1.49 ppm). The three highest environment levels were in different regions; spinal cord removal (1.13 ppm), lower limb (0.72 ppm), and thorax (0.71 ppm) dissection. Only the perineum environment level (0.09 ppm) was below the NIOSH ceiling level (0.1 ppm), which may result from the table covers that had been opened for 2 weeks before measurement. This study finding signified the importance of student personal exposure monitoring and encouraged the academic year measurement. Because each laboratory has unique factors, those affect formaldehyde levels; dissection steps, dissection table design, cadaver storage protocol, and heating-ventilation-air conditioning system performance, for instance.