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Acoustic stress responses in juvenile sea bass Dicentrarchus labrax induced by offshore pile driving
2016
Underwater sound generated by pile driving during construction of offshore wind farms is a major concern in many countries. This paper reports on the acoustic stress responses in young European sea bass Dicentrarchus labrax (68 and 115 days old), based on four in situ experiments as close as 45 m from a pile driving activity. As a primary stress response, whole-body cortisol seemed to be too sensitive to ‘handling’ bias. On the other hand, measured secondary stress responses to pile driving showed significant reductions in oxygen consumption rate and low whole-body lactate concentrations. Furthermore, repeated exposure to impulsive sound significantly affected both primary and secondary stress responses. Under laboratory conditions, no tertiary stress responses (no changes in specific growth rate or Fulton's condition factor) were noted in young sea bass 30 days after the treatment. Still, the demonstrated acute stress responses and potentially repeated exposure to impulsive sound in the field will inevitably lead to less fit fish in the wild.
Mostrar más [+] Menos [-]Acute phenanthrene toxicity to juvenile diploid and triploid African catfish (Clarias gariepinus): Molecular, biochemical, and histopathological alterations
2016
Karamī, ʻAlī | Romano, Nicholas | Hamzah, Hazilawati | Simpson, Stuart L. | Yap, Chee Kong
Information on the biological responses of polyploid animals towards environmental contaminants is scarce. This study aimed to compare reproductive axis-related gene expressions in the brain, plasma biochemical responses, and the liver and gill histopathological alterations in diploid and triploid full-sibling juvenile African catfish (Clarias gariepinus). Fish were exposed for 96 h to one of the two waterborne phenanthrene (Phe) concentrations [mean measured (SD): 6.2 (2.4) and 76 (4.2) μg/L]. In triploids, exposure to 76 μg/L Phe increased mRNA level of fushi tarazu-factor 1 (ftz-f1). Expression of tryptophan hydroxylase2 (tph2) was also elevated in both ploidies following the exposure to 76 μg/L Phe compared to the solvent control. In triploids, 76 μg/L Phe increased plasma alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) levels compared to the other Phe-exposed group. It also elevated lactate and glucose contents relative to the other groups. In diploids, however, biochemical biomarkers did not change. Phenanthrene exposures elevated glycogen contents and the prevalence of histopathological lesions in the liver and gills of both ploidies. This study showed substantial differences between diploids and triploids on biochemical and molecular biomarker responses, but similar histopathological alterations following acute Phe exposures.
Mostrar más [+] Menos [-]Advances in understanding the mechanisms of mercury toxicity in wild golden grey mullet (Liza aurata) by 1H NMR-based metabolomics
2016
Cappello, Tiziana | Pereira, Patrícia | Maisano, Maria | Mauceri, Angela | Pacheco, Mario | Fasulo, Salvatore
Mercury (Hg) is recognized as a dangerous contaminant due to its bioaccumulation and biomagnification within trophic levels, leading to serious health risks to aquatic biota. Therefore, there is an urgent need to unravel the mechanisms underlying the toxicity of Hg. To this aim, a metabolomics approach based on protonic nuclear magnetic resonance (1H NMR), coupled with chemometrics, was performed on the gills of wild golden grey mullets L. aurata living in an Hg-polluted area in Ria de Aveiro (Portugal). Gills were selected as target organ due to their direct and continuous interaction with the surrounding environment. As a consequence of accumulated inorganic Hg and methylmercury, severe changes in the gill metabolome were observed, indicating a compromised health status of mullets. Numerous metabolites, i.e. amino acids, osmolytes, carbohydrates, and nucleotides, were identified as potential biomarkers of Hg toxicity in fish gills. Specifically, decrease of taurine and glycerophosphocholine, along with increased creatine level, suggested Hg interference with the ion-osmoregulatory processes. The rise of lactate indicated anaerobic metabolism enhancement. Moreover, the increased levels of amino acids suggested the occurrence of protein catabolism, further supported by the augmented alanine, involved in nitrogenous waste excretion. Increased level of isobutyrate, a marker of anoxia, was suggestive of onset of hypoxic stress at the Hg contaminated site. Moreover, the concomitant reduction in glycerophosphocholine and phosphocholine reflected the occurrence of membrane repair processes. Finally, perturbation in antioxidant defence system was revealed by the depletion in glutathione and its constituent amino acids. All these data were also compared to the differential Hg-induced metabolic responses previously observed in liver of the same mullets (Brandão et al., 2015). Overall, the environmental metabolomics approach demonstrated its effectiveness in the evaluation of Hg toxicity mechanisms in wild fish under realistic environmental conditions, uncovering tissue-specificities regarding Hg toxic effects namely in gills and liver.
Mostrar más [+] Menos [-]Enhanced Reduction of p-Nitrophenol by a Methanogenic Consortium Promoted by Metallic Nanoparticles
2016
Cervantes, Francisco J. | Rodríguez López, José Luis | Peña Martínez, Mariana | Ascacio-Valdés, Juan Alberto | Aguilar, Cristobal N. | Bahena, Daniel
The present study reports the synthesis and characterization of metallic nanoparticles (NPs) of Pd and bimetallic alloys of PdCu NPs for their application as catalysts to achieve the microbial reduction of p-nitrophenol (PNP). Addition of bimetallic alloys of PdCu NPs to methanogenic sludge incubations increased up to threefold the rate of reduction of PNP. Moreover, their presence promoted a more efficient and selective reduction of PNP to the desired product (p-aminophenol) with negligible accumulation of toxic intermediates (p-nitroso-phenol and p-hydroxylamine-phenol), which prevailed in sludge incubations lacking nanocatalysts. PdCu NPs synthesized by adding precursors H₂PdCl₄ and H₂CuCl₄ independently and simultaneously to the synthesis vessel showed superior catalytic properties as compared to those produced by mixing the same precursors prior addition to the synthesis vessel. The enhanced catalytic properties of bimetallic NPs could be explained by higher physical stability and interfacial arrangement within PdCu alloys promoting a more efficient transfer of reducing equivalents derived from lactate/ethanol fermentation towards the target nitro group in PNP. A wastewater treatment technology, combining the microbial activity of methanogenic consortia and the catalytic activity of bimetallic NPs, is proposed as an alternative for the removal of recalcitrant pollutants from wastewaters.
Mostrar más [+] Menos [-]Performance and Bacterial Community Shifts During Phosphogypsum Biotransformation
2016
Martins, Monica | Assunção, Ana | Neto, André | Silva, Gonçalo | Sghaier, Haïtham | Costa, MariaClara
Phosphogypsum (PG) is an industrial waste composed mainly by sulfate, turning it a suitable sulfate source for sulfate-reducing bacteria (SRB). In the present work, the capability of two SRB communities, one enriched from Portuguese PG (culture PG) and the other from sludge from a wastewater treatment plant (culture WWT-1), to use sulfate from PG was compared. In addition, the impact of this sulfate-rich waste in the microbial community was assessed. The highest efficiency in terms of sulfate reduction was observed with culture WWT-1. The bacterial composition of this culture was not significantly affected when sodium sulfate from the nutrient medium was replaced by PG as a sulfate source. Next generation sequencing (NGS) showed that this community was phylogenetically diverse, composed by bacteria affiliated to Clostridium, Arcobacter, and Sulfurospirillum genera and by SRB belonging to Desulfovibrio, Desulfomicrobium, and Desulfobulbus genera. In contrast, the bacterial structure of the community enriched from PG was modified when sodium sulfate was replaced by PG as the sulfate source. This culture, which showed the poorest performance in the use of sulfate from PG, was mainly composed by SRB related to Desulfosporosinus genus. The present work provides new information regarding the phylogenetic characterization of anaerobic bacterial communities with the ability to use PG as sulfate donor, thus, contributing to improve the knowledge of microorganisms suitable to be used in PG bioremediation. Additionally, this paper demonstrates that an alternative to lactate and low-cost carbon source (wine wastes) can be used efficiently for that purpose.
Mostrar más [+] Menos [-]Investigation of ethyl lactate as a green solvent for desorption of total petroleum hydrocarbons (TPH) from contaminated soil
2016
Jalilian Ahmadkalaei, Seyedeh Pegah | Gan, Suyin | Ng, Hoon Kiat | Abdul Talib, Suhaimi
Treatment of oil-contaminated soil is a major environmental concern worldwide. The aim of this study is to examine the applicability of a green solvent, ethyl lactate (EL), in desorption of diesel aliphatic fraction within total petroleum hydrocarbons (TPH) in contaminated soil and to determine the associated desorption kinetics. Batch desorption experiments were carried out on artificially contaminated soil at different EL solvent percentages (%). In analysing the diesel range of TPH, TPH was divided into three fractions and the effect of solvent extraction on each fraction was examined. The experimental results demonstrated that EL has a high and fast desorbing power. Pseudo-second order rate equation described the experimental desorption kinetics data well with correlation coefficient values, R ², between 0.9219 and 0.9999. The effects of EL percentage, initial contamination level of soil and liquid to solid ratio (L/S (v/w)) on initial desorption rate have also been evaluated. The effective desorption performance of ethyl lactate shows its potential as a removal agent for remediation of TPH-contaminated soil worldwide.
Mostrar más [+] Menos [-]Effect of carbon sources on the aggregation of photo fermentative bacteria induced by L-cysteine for enhancing hydrogen production
2016
Xie, Guo-Jun | Liu, Bing-Feng | Ding, Jie | Wang, Qilin | Ma, Chao | Zhou, Xu | Ren, Nan-Qi
Poor flocculation of photo fermentative bacteria resulting in continuous biomass washout from photobioreactor is a critical challenge to achieve rapid and stable hydrogen production. In this work, the aggregation of Rhodopseudomonas faecalis RLD-53 was successfully developed in a photobioreactor and the effects of different carbon sources on hydrogen production and aggregation ability were investigated. Extracellular polymeric substances (EPS) production by R. faecalis RLD-53 cultivated using different carbon sources were stimulated by addition of L-cysteine. The absolute ζ potentials of R. faecalis RLD-53 were considerably decreased with addition of L-cysteine, and aggregation barriers based on DLVO dropped to 15–43 % of that in control groups. Thus, R. faecalis RLD-53 flocculated effectively, and aggregation abilities of strain RLD-53 cultivated with acetate, propionate, lactate and malate reached 29.35, 32.34, 26.07 and 24.86 %, respectively. In the continuous test, hydrogen-producing activity was also promoted and reached 2.45 mol H₂/mol lactate, 3.87 mol H₂/mol propionate and 5.10 mol H₂/mol malate, respectively. Therefore, the aggregation of R. faecalis RLD-53 induced by L-cysteine is independent on the substrate types, which ensures the wide application of this technology to enhance hydrogen recovery from wastewater dominated by different organic substrates.
Mostrar más [+] Menos [-]The effect of U speciation in cultivation solution on the uptake of U by variant Sedum alfredii
2016
Du, Liang | Feng, Xiaojie | Huang, Zhenling | Liu, Borui | Jin, Yongdong | Fang, Zhenggang | Zhang, Dong | Liu, Ning | Wang, Ruibing | Xia, Chuanqin
In the present study, five plant species were screened for uranium uptake using a hydroponic experimental set-up. The effect of the U concentration, pH, as well as the presence of carbonates, phosphates, and organic acids (lactic acid, malic acid, citric acid) on the uptake of U by variant S. alfredii (V S. alfredii) and wild S. alfredii (W S. alfredii) were investigated. Results showed that V S. alfredii exhibited higher U content in the roots than the other four plants and with the increase of U concentration in the solution, the U uptake by V S. alfredii and W S. alfredii increased. The results also showed that different U speciation in different cultivation solution took an important role on the uptake of U in variant Sedum alfredii: at pH 6.5, U hydrolysis species (UO₂)₃(OH)₅ ⁺is predominant and the U concentrations in V S. alfredii roots reached a maximum value (3.7 × 10⁴ mg/kg). U complexation with carbonates, phosphates, and some organic acids in the solution resulted in a decrease in the U content in the roots except for lactic acid. Our researches highlight the correlations between U speciation and the uptake on V S. Alfredii, which will be helpful for improved removal of U from the groundwater using phytoremediation method.
Mostrar más [+] Menos [-]Drugs of environmental concern modify Solea senegalensis physiology and biochemistry in a temperature-dependent manner
2016
González-Mira, A | Varó, I | Solé, M | Torreblanca, A
The alerted presence in recent decades of pharmaceuticals has become an issue of environmental concern, and most of the mechanisms of biotransformation and biochemical and physiological responses to them in fish are still unknown, as well as the influence of water temperature in their ability to cope with them. This study aims to detect the main effects of two of the most widespread drugs on a set of physiological and biochemical markers in Solea senegalensis. Sole juveniles acclimatized at 15 and 20 °C were administered an intraperitoneal injection of the non-steroidal anti-inflammatory drug ibuprofen (IB; 10 mg/kg) and the anti-convulsant drug carbamazepine (CBZ; 1 mg/kg). Two days after the injection, liver, muscle and plasma were sampled. Liver enzymatic activities of 15 °C acclimated fish were more responsive to pharmaceuticals than those acclimated at 20 °C, especially for CYP450-related activities (7-ethoxyresorufin (EROD), 7-methoxyresorufin (MROD), 3-cyano-7-ethoxycoumarin (CECOD) and 7-benzyloxy-4-[trifluoromethyl]-coumarin-O-debenzyloxylase (BFCOD)) and uridine diphosphate glucuronosyltransferase (UDPGT). Cytosolic anti-oxidant enzyme activities and glutathione S-transferase (GST) did not show a clear effect of temperature. Glucose and transferase activities in plasma were not affected by the treatments, while ammonium, osmolality and lactate were affected by both pharmaceuticals. Plasma triglycerides were affected in a temperature-dependent manner, and creatinine was only responsive to CBZ injection. HSP70 levels in muscle were only affected by CBZ injection. Some of the physiological identified responses to IB and CBZ are proposed as endpoints in further chronic studies.
Mostrar más [+] Menos [-]Organic acid compounds in root exudation of Moso Bamboo (Phyllostachys pubescens) and its bioactivity as affected by heavy metals
2016
Chen, Junren | Shafi, Mian M. | Wang, Ying | Wu, Jiasen | Ye, Zhengqian | Liu, Chen | Zhong, Bin | Guo, Hua | He, Lizhi | Liu, Dan
Moso bamboo (Phyllostachys pubescens) has great potential as phytoremediation material in soil contaminated by heavy metals. A hydroponics experiment was conducted to determine organic acid compounds of root exudates of lead- (Pb), zinc- (Zn), copper- (Cu), and cadmium (Cd)-tolerant of Moso bamboo. Plants were grown in nutrients solution which included Pb, Zn, Cu, and Cd applied as Pb(NO₃)₂ (200 μM), ZnSO₄·7H₂O (100 μM), CuSO₄·5H₂O (25 μM), and CdCl₂ (10 μM), respectively. Oxalic acid and malic acid were detected in all treatments. Lactic acid was observed in Cu, Cd, and control treatments. The oxalic was the main organic acid exudated by Moso bamboo. In the sand culture experiment, the Moso bamboo significantly activated carbonate heavy metals under activation of roots. The concentration of water-soluble metals (except Pb) in sand were significantly increased as compared with control. Organic acids (1 mM mixed) were used due to its effect on the soil adsorption of heavy metals. After adding mixed organic acids, the Cu and Zn sorption capacity in soils was decreased markedly compared with enhanced Pb and Cd sorption capacity in soils. The sorption was analyzed using Langmuir and Freundlich equations with R ² values that ranged from 0.956 to 0.999 and 0.919 to 0.997, respectively.
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