Bisphenol A (BPA) is a harmful environmental contaminant acting as an endocrine disruptor in animals, but it also affects growth and development in plants. Here, we have elucidated the functional mechanism of root growth inhibition by BPA in Arabidopsis thaliana using mutants, reporter lines and a pharmacological approach. In response to 10 ppm BPA, fresh weight and main root length were reduced, while auxin levels increased. BPA inhibited root growth by reducing root cell length in the elongation zone by suppressing expansin expression and by decreasing the length of the meristem zone by repressing cell division. The inhibition of cell elongation and cell division was attributed to the enhanced accumulation/redistribution of auxin in the elongation zone and meristem zone in response to BPA. Correspondingly, the expressions of most auxin biosynthesis and transporter genes were enhanced in roots by BPA. Taken together, it is assumed that the endocrine disruptor BPA inhibits primary root growth by inhibiting cell elongation and division through auxin accumulation/redistribution in Arabidopsis. This study will contribute to understanding how BPA affects growth and development in plants.

Despite the large number of studies reporting the phytotoxicity of graphene-based materials, the effects of these materials on nutrient uptake in plants remain unclear. The present study showed that nitrate concentrations were significantly decreased in the roots of wheat plants treated with graphene oxide (GO) at 200–800 mg L⁻¹. Non-invasive microelectrode measurement demonstrated that GO could significantly inhibit the net NO₃⁻ influx in the meristematic, elongation, and mature zones of wheat roots. Further analysis indicated that GO could be trapped in the root vacuoles, and that the maximal root length and the number of lateral roots were significantly reduced. Additionally, root tip whitening, creases, oxidative stress, and weakened respiration were observed. These observations indicate that GO is highly unfavorable for vigorous root growth and inhibits increase in root uptake area. At the molecular level, GO exposure caused DNA damage and inhibited the expression of most nitrate transporters (NRTs) in wheat roots, with the most significantly downregulated genes being NRT1.3, NRT1.5, NRT2.1, NRT2.3, and NRT2.4. We concluded that GO exposure decreased the root uptake area and root activity, and decreased the expression of NRTs, which may have consequently suppressed the NO₃⁻ uptake rate, leading to adverse nitrate accumulation in stressed plants.

Copper (Cu) induced phytotoxicity has become a serious environmental problem as a consequence of significant metal release through anthropogenic activity. Understanding the spatial distribution of Cu in plants such as willow is essential to elucidate the mechanisms of metal accumulation and transport in woody plants, particularly as affected by variable environment conditions such as soil flooding. Using synchrotron-based X-ray fluorescence (μ-XRF) techniques, the spatial distribution of Cu and other nutrient elements were investigated in roots and stems of Salix (S.) integra exposed to 450 mg kg⁻¹ Cu under non-flooded (NF)/flooding (F) conditions for 90 d. S. integra grown in the F condition exhibited significant higher tolerance index (TI, determined by the ratio of total biomass in Cu treatments to control) (p < 0.05) than that in the NF condition, indicating soil flooding alleviated Cu toxicity to willow plants. The μ-XRF revealed that Cu was preferentially located in the root cap and meristematic zone of the root tips. Under the NF condition, the Cu intensity in the root epidermis was more highly concentrated than that of the F condition, suggesting the soil flooding significantly inhibited Cu uptake by S. integra. The pattern of the Cu spatial distribution in the S. integra stem indicated that the F condition severely reduced Cu transport via the xylem vessels as a consequence of decreasing the transpiration rate of leaves. To our knowledge, this is the first study to report the in vivo Cu distribution in S. integra in a scenario of co-exposure to the Cu and the soil flooding over a long period. The finding that Cu uptake varies significantly with flooding condition is relevant to the development of strategies for plants to detoxify the metals and to maintain the nutrient homeostasis.

The current study provides an information on the combined effect of pollution with potentially toxic elements (PTEs) and polycyclic aromatic hydrocarbons (PAHs) in hydromorphic soils on the accumulation, growth, functional and morphological-anatomical changes of macrophyte plant, i.e., Phragmites australis Cav., as well as information about their bioindication status on the example of small rivers of the Azov basin. The territory of the lower reaches of the Kagalnik River is one of the small rivers of the Eastern Azov region was examined with different levels of PTEs contamination in soils, where the excess of the lithosphere clarkes and maximum permissible concentrations (MPC) for Mn, Cr, Zn, Pb, Cu, and Cd were found. The features of the 16 priority PAHs quantitative and qualitative composition in hydromorphic soils and P. australis were revealed. The influence of soil pollution on accumulation in P. australis, as well as changes in the morphological parameters were shown. It has been observed that morphometric changes in P. australis at sites experiencing the сontamination and salinity are reflected with the changes in the ultrastructure of plastids, mitochondria, and EPR elements of plant cells. PTEs accumulated in inactive organs and damaged cell structures. At the same time, PAHs penetrated through the biomembranes and violated their integrity, increased permeability, resulted cell disorganization, meristem, and conductive tissues of roots. The nature and extent of the structural alterations found are dependent on the type and extent of pollution in the examined regions and can be utilized as bioindicators for evaluating the degree of soil phytotoxicity characterized by the accumulation of PTE and PAHs.

Silver nanoparticles (AgNPs) are widely used in consumer products due to their antibacterial property; however, their potential toxicity and release into the environment raises concern. Based on the limited understanding of AgNPs aggregation behavior, this study aimed to investigate the toxicity of uncoated (uc-AgNP) and coated with polyvinylpyrrolidone (PVP-AgNP), at low concentrations (0.5–100 ng/mL), under dark and visible-light exposure, using a plant test system. We exposed Allium cepa seeds to both types of AgNPs for 4–5 days to evaluate several toxicity endpoints. AgNPs did not cause acute toxicity (i.e., inhibition of seed germination and root development), but caused genotoxicity and biochemical alterations in oxidative stress parameters (lipid peroxidation) and activities of antioxidant enzymes (superoxide dismutase and catalase) in light and dark conditions. However, the light exposure decreased the rate of chromosomal aberration and micronuclei up to 5.60x in uc-AgNP and 2.01x in PVP-AgNP, and 2.69x in uc-AgNP and 3.70x in PVP-AgNP, respectively. Thus, light exposure reduced the overall genotoxicity of these AgNPs. In addition, mitotic index alterations and morphoanatomical changes in meristematic cells were observed only in the dark condition at the highest concentrations, demonstrating that light also reduces AgNPs cytotoxicity. The light-dependent aggregation of AgNPs may have reduced toxicity by reducing the uptake of these NPs by the cells. Our findings demonstrate that AgNPs can be genotoxic, cytotoxic and induce morphoanatomical and biochemical changes in A. cepa roots even at low concentrations, and that visible-light alters their aggregation state, and decreases their toxicity. We suggest that visible light can be an alternative treatment to remediate AgNP residues, minimizing their toxicity and environmental risks.

The uptake of polycyclic aromatic hydrocarbons and their cellular effects were investigated in the mangrove Bruguiera gymnorrhiza. Seedlings were subjected to sediment oiling for three weeks. In the oiled treatment, the ƩPAHs was higher in roots (99%) than in leaves (1%). In roots, PAHs included phenanthrene (55%), acenaphthene (13%), fluorine (12%) and anthracene (8%). In leaves, PAHs possessed two to three rings and included acenaphthene (35%), naphthalene (33%), fluorine (18%) and phenanthrene (14%). In the roots, oil caused disorganization of cells in the root cap, meristem and conducting tissue. Oil contaminated cells were distorted and possessed large and irregularly shaped vacuoles. Ultrastructural changes included loss of cell contents and fragmentation of the nucleus and mitochondrion. In the leaves, oil caused dilation and distortion of chloroplasts and disintegration of grana and lamellae. Oil targets critical organelles such as nuclei, chloroplasts and mitochondria which are responsible for cell vitality and energy transformation.

The immense protection potential of plant-derived products against heavy metal toxicity has become a considerable field of research. The goal of the present study was to evaluate the mitigative ability of turmeric against nickel (II) chloride (NiCl₂)-related toxicity in the roots of Allium cepa L. For this purpose, one control (treated with tap water) and five treatment groups (treated with 440 mg/L turmeric, 880 mg/L turmeric, 1 mg/L NiCI₂, 1 mg/L NiCI₂ + 440 mg/L turmeric, and 1 mg/L NiCI₂ + 880 mg/L turmeric, respectively) of Allium bulbs were established. Experimental conditions were maintained at room temperature for 3 days. Physiological, biochemical, cytogenetic, and meristematic integrity parameters were analyzed in all groups. NiCl₂ reduced germination percentage, root elongation, and weight gain. Following NiCl₂ application, the frequency of aberrant chromosomes and micronuclei increased, while mitotic index decreased. NiCl₂ caused an increase in oxidative stress, which was evident by increased malondialdehyde level and catalytic activities of superoxide dismutase and catalase. Epidermal and cortex cell injuries as well as deformed cell nuclei and indistinct transmission tissue were observed as a result of NiCl₂ treatment. When applied alone, turmeric, which did not cause any negative effects, led to an improvement in all parameters depending on the dose when applied together with NiCl₂. Data from the study suggests that turmeric has remarkable protection potential against NiCl₂ in Allium cepa.

Mercury (Hg) is a highly toxic heavy metal for all organisms. In the present study, the mitigative role of 190 mg/L and 380 mg/L doses of green tea extract (GTex) against mercury(II) chloride (HgCI₂)-induced toxicity was evaluated in Allium cepa L. For this aim, selected physiological, genotoxicity, and biochemical parameters as well as meristematic cell injuries in the roots were investigated. Ratios of catechin and caffeine in GTex were determined by HPLC analysis. Also, free radical scavenging activity of GTex was tested against superoxide and hydrogen peroxide radicals. As a result of HgCI₂ application, germination percentage, root elongation, weight gain, and mitotic index (MI) declined, while the frequency of micronucleus (MN), chromosomal abnormalities (CAs), and meristematic cell damages increased. HgCI₂ administration also led to a significant increase in malondialdehyde content, superoxide dismutase, and catalase activities which are signs of oxidative stress. On contrary, applications of GTex together with HgCI₂ reduced HgCI₂-induced adverse effects in all parameters in a dose-dependent manner. Antioxidant components in GTex were listed as caffeine, epigallocatechin gallate, epigallocatechin, epicatechin gallate, and catechin according to their abundance. GTex exhibited a strong scavenging ability in the presence of superoxide and hydrogen peroxide radicals. The present study revealed the strong protective capacity of GTex against HgCI₂-induced toxicity in A. cepa owing to its high antioxidant content with a multifaceted perspective. With this study, a reliable starting point was established for future studies investigating the more common and diverse use of GTex against toxic substances.

The disposal of carmine cochineal and caramel IV dyes into the environment through effluents generated in the industry is constant. However, studies on the toxicity caused to terrestrial plants and aquatic organisms are limited for the carmine dye and non-existent for the caramel dye. The aim of this study was to evaluate the cytotoxicity and genotoxicity of color additives in meristematic Allium cepa cells from 22.50 to 0.225 mL/L for the carmine cochineal dye and from 18.00 to 0.045 mL/L for the caramel IV dye. In A. cepa, we considered the concentration of each dye in the effluent after the usual biological degradation carried out in the industry. Data were tested by using analysis of variance (one-way ANOVA), and the mean values were compared by using the Scott-Knott test with a significance of 0.05. Cochineal dye at concentrations from 22.50 to 2.81 mL/L and caramel dye at concentrations from 18.00 to 1.125 mL/L were cytotoxic. Cochineal dye was not genotoxic. Caramel concentrations 2.25 and 0.09 mL/L were genotoxic. The toxicity of each dye was also evaluated against Artemia salina at concentrations from 1000 to 0.97 ppm, determining the LC₅₀. The analyzed concentrations were defined based on the standard protocol for checking the mortality rate in brine shrimp. Concentration that causes mortality of 50% larvae was calculated based on the equation of the line obtained by linear regression. The additives were very toxic to A. salina with LC₅₀ = 1.72 ppm for carmine additive and LC₅₀ = 3.88 ppm for caramel. The dyes evaluated in this study indicate the potential to cause damage to organisms in the environment.

In consideration of the hazards associated with the presence of the textile azo-dye and their biotransformation products in the environment, the goal of this work was to study bioremediation process by the yeast strain Pichia kudriavzevii CR-Y103 related to the ability to degrade and detoxify the sulfonated Reactive Orange 16 azo-dye. In experimental conditions, the optimal inoculum/dye concentration ratio required for complete decolorization (100%) of culture medium and biomass within 24 h has been 1 g L⁻¹ yeast cell (dry weight)/50 mg L⁻¹ Reactive Orange 16. In the presence of 400 mg L⁻¹ of Reactive Orange 16 (RO16), 95% of the dye was removed after 72 h of incubation. Also, the yeast strain could decolorize other eight textile dyes (56.48–99.98% decolorization within 24 h). NADH-DCIP reductase and azo reductase activities were significantly increased (ca. 5.4 times and ca. 37 times, respectively) during the decolorization process. UV-VIS spectra, high-performance liquid chromatography (HPLC), and Fourier transform infrared spectroscopy (FTIR) analysis confirmed the presence of new biotransformation products in extracted metabolites, highlighting the partial biodegradation of the dye by the new yeast isolate. The phytotoxicity evaluation strongly supported the decreased toxicity of biodegraded products as minor inhibition on germination (%), root and shoots elongation of T. pratense L. and T. aestivum L. seedlings. Increasing of mitotic index value and decreasing the frequency of chromosomal aberrations in tested plant meristem cells treated with biodegraded products, compared with RO16 treatment (500 ppm), confirmed their slightly toxic nature. A cell viability assay also confirmed the reduced toxicity of biodegraded products on healthy monkey kidney cells (Vero cells).