In this study, the removal of nitrogen from effluent of ammonia plant by Chlorella vulgaris and Spirulina platensis was investigated. For this purpose, microalgae were cultivated in three diluting percentage of the wastewater (1, 3, and 5%) at 29±1 ◦C and light intensity at surface of culture were adjusted to 150 µmol photon / (m2. s). The results showed that Spirulina platensis is more capable than Chlorella vulgaris to grow in high levels of total nitrogen concentration. Also, maximum biomass production rate happened in 1% diluted samples for Chlorella vulgaris and 3% for Spirulina platensis. Furthermore, Chlorella vulgaris reduce total nitrogen concentration up to 55%. This value for Spirulina platensis was about 96%. However, for both species the removal of nitrogen in 1% diluted wastewater was maximum. According to the results of diluted wastewater of ammonia plant, it is a suitable culture medium for microalgae and it can be used to remove the nitrogen before entering the wastewater in nature.

Worldwide there is an immense demand for plastic material that results in “white pollution”. Petrochemical-based plastic is used all over the world which leads to adverse impacts on every sphere of the earth. However, many steps have been taken to control this plastic pollution globally, such as chemical treatments, plastic waste incineration, sanitary landfilling, and 7 R programs. Still, plastic pollution is one of the major international problems. Non-biodegradable plastic would not eradicate from our environment until we have an economically feasible and more biodegradable substitute. In recent years algae, especially microalgae, have got attention worldwide, owing to their various applications. Microalgae is one of the sustainable ways of bioplastic synthesis as during cultivation it also purifies wastewater. This review paper has summarized various species of microalgae used for the synthesis of bioplastic, their cultivation system, and methods for bioplastic production by using microalgae biomass, followed by multiple challenges, solutions, and future prospects.

Corrosion is an environmental and economic global problem. “Smart” or stimuli-responsive colorimetric nanosensors for maritime coatings have been proposed as an asset to overcome the limitations of the current monitoring techniques by changing color in the presence of triggers associated with the early stages of corrosion. Layered double hydroxides (Zn–Al LDH; Mg–Al LDH) and silica mesoporous nanocapsules (SiNC) were used as precursor nanocarriers of active compounds: hexacyanoferrate ions ([Fe(CN)₆]³⁻) and phenolphthalein (PhPh), respectively. Additionally, the safer-by-design principles were employed to optimize the nanosensors in an eco-friendly perspective (e.g., regular vs. warm-washed SiNC-PhPh; immobilization using different carriers: Zn–Al LDH-[Fe(CN)₆]³⁻ vs. Mg–Al LDH-[Fe(CN)₆]³⁻). Therefore, the present study aims to assess the environmental behavior in saltwater and the toxic effects of the nanosensors, their nanocarriers, and the active compounds on the marine microalgae Tetraselmis chuii and the crustacean Artemia salina. Briefly, tested compounds exhibited no acute toxic effects towards A. salina (NOEC = 100 mg/L), apart from SiNC-PhPh (LC₅₀ = 2.96 mg/L) while tested active compounds and nanosensors caused significant growth inhibition on T. chuii (lowest IC₅₀ = 0.40 mg/L for SiNC-PhPh). The effects of [Fe(CN)₆]³⁻ were similar regardless of the nanocarrier choice. Regarding SiNC-PhPh, its toxicity can be decreased at least twice by simply reinforcing the nanocapsules washing, which contributes to the removal (at least partially) of the surfactants residues. Thus, implementing safe-by-design strategies in the early stages of research proved to be critical, although further progress is still needed towards the development of truly eco-friendly nanosensors.

Batch sorption of metformin hydrochloride (MET) onto a specially designed biochar mix consisting of both macro (MAC) and micro (MIC) algae, rice husk and pine sawdust was conducted. Pyrolysis of both MAC and MIC algae mixture was done followed by chemical activation with hydrogen-peroxide. Additionally, sorption of MET under the influence of pH was separately investigated. Batch studies of isotherms were well described by Freundlich model with high non-linearity and Freundlich exponent values ranged anywhere from 0.12 to 1.54. Heterogeneity of MET adsorption to the bonding sites was attributed to the surface functional groups of the modified biochar. Amongst the four biochars, the activated macroalgae biochar (MACAC) and microalgae biochar (MICAC) depicted favourable adsorption of MET with maximum adsorption at pH 7. Up to 76% of MET removal from the environment was obatained using the MACAC biochar. Scanning electron micrographs coupled with energy dispersive X-ray, as well as elemental analyses confirmed formation of oxygen containing surface functional groups due to activation strengthening chemisorption as the main sorption mechanism. Further, Fourier transform infra-red spectroscopy and other surface functional group analyses along with Zeta potential measurements reinforced our proposed sorption mechanism. Lowest zeta potential observed at pH 7 enhanced the electrostatic force of attraction for both the biochars. Negative zeta potential value of the biochars under different pH indicated potential of the biochars to adsorb other positively charged contaminants. From a techno-economic perspective, capital expenditure cost is not readily available, however, it is envisaged that production of pyrolyzed biochar from algal biomass could make the process economically attractive especially when the biochar could be utilised for high-end applications.

Bibliometric network analysis has revealed that the widespread distribution of microplastics (MPs) has detrimental effects on marine organisms; however, the combined effects of MPs and climate change (e.g., warming) is not well understood. In this study, Prorocentrum donghaiense, a typical red tide species in the East China Sea, was exposed to different MP concentrations (0, 1, 5, and 10 mg L⁻¹) and temperatures (16, 22, and 28 °C) for 7 days to investigate the combined effects of MPs and simulated ocean warming by measuring different physiological parameters, such as cell growth, pigment contents (chlorophyll a and carotenoid), relative electron transfer rate (rETR), reactive oxygen species (ROS), superoxide dismutase (SOD), malondialdehyde (MDA), and adenosine triphosphate (ATP). The results demonstrated that MPs significantly decreased cell growth, pigment contents, and rETRₘₐₓ, but increased the MDA, ROS, and SOD levels for all MP treatments at low temperature (16 °C). However, high temperatures (22 and 28 °C) increased the pigment contents and rETRₘₐₓ, but decreased the SOD and MDA levels. Positive and negative effects of high temperatures (22 or 28 °C) were observed at low (1 and 5 mg L⁻¹) and high MP (10 mg L⁻¹) concentrations, respectively, indicating the antagonistic and synergistic effects of combined warming and MP pollution. These results imply that the effects of MPs on microalgae will likely not be substantial in future warming scenarios if MP concentrations are controlled at a certain level. These findings expand the current knowledge of microalgae in response to increasing MP pollution in future warming scenarios.

Heterosigma akashiwo is a commonly found harmful microalgae, however, there are only few studies on its control using algicidal components particularly those identified from algicidal bacteria. In our previous study, ortho-tyrosine and urocanic acid identified from Bacillus sp. B1 showed a significantly high algicidal effect on H. akashiwo. The growth inhibition rates of H. akashiwo after 96 h of treatment with 300 μg/mL o-tyrosine and 500 μg/mL urocanic acid were 91.06% and 88.07%, respectively. Through non-destructive testing by Pulse Amplitude Modulation fluorometry and flow cytometer, the effects of o-tyrosine and urocanic acid on H. akashiwo PS II and physiological parameters (cell volume, mitochondrial membrane potential, and membrane permeability) were estimated. This study shows that o-tyrosine affected the photosynthesis system of H. akashiwo, decreased the mitochondrial membrane potential, and increased the membrane permeability of the algal cells. Treatment with urocanic acid decreased the mitochondrial membrane potential, resulting in the inhibition of algal cell growth and reproduction, but had little effect on membrane permeability and photosynthetic system. Our results may imply that when uridine degrades, surviving H. akashiwo cells may be reactivated. Therefore, o-tyrosine and urocanic acid have the potential to become new biological algicides, which can effectively control the growth of H. akashiwo.

With the fast growth of the production and application of engineered nanomaterials (ENMs), nanoparticles (NPs) that escape into the environment have drawn increasing attention due to their ecotoxicological impacts. Motile microalgae are a type of primary producer in most ecosystems; however, the impacts of NPs on the motility of microalgae have not been studied yet. So the toxic impacts of three common metal oxide NPs (nTiO₂, nZnO, and nFe₂O₃) on swimming speed and locomotion mode of a marine microalgae, Platymonas subcordiformis, were investigated in this study. Our results demonstrated that both the velocity and linearity (LIN) of swimming were significantly decreased after the exposure of P. subcordiformis to the tested NPs. In addition, the obtained data indicate that NPs may suppress the motility of P. subcordiformis by constraining the energy available for swimming, as indicated by the significantly lower amounts of intracellular ATP and photosynthetic pigments and the lower activities of enzymes catalyzing glycolysis. Incubation of P. subcordiformis with the tested NPs generally resulted in the overproduction of reactive oxygen species (ROS), aggravation of lipid peroxidation, and induction of antioxidant enzyme activities, suggesting that imposing oxidative stress, which may impair the structural basis for swimming (i.e. the membrane of flagella), could be another reason for the observed motility suppression. Moreover, NP exposure led to significant reductions in the cell viability of P. subcordiformis, which may be due to the disruption of the energy supply (i.e., photosynthesis) and ROS-induced cellular damage. Our results indicate that waterborne NPs may pose a great threat to motile microalgae and subsequently to the health and stability of the marine ecosystem.

Salinity stress affects aquatic microalgal growth and their physiological responses have been studied extensively. However, arsenic (As) accumulation and biotransformation by freshwater phytoplankton under a salinity gradient have never been addressed. This study reports a distinctive pattern of As uptake, accumulation, and biotransformation by four axenic freshwater phytoplankton species, i.e., Scenedesmus acutus, Closterium aciculare, Staurastrum paradoxum, and Pediastrum duplex. Phytoplankton cells were incubated in sterilised C medium modified with varying salinity levels (0–5‰) in association with arsenate and phosphate concentrations. The biotransformation of arsenate (i.e., As(V)) to arsenite (As(III)) and to further methylated species decreased with increasing salinity in the culture medium whereas As accumulation increased. Among the four strains, only S. acutus and S. paradoxum converted As(V) to As(III), with no detected methylated species. In contrast, C. aciculare and P. duplex biotransformed As(V) to As(III) and further to methyl arsenic species, such as DMAA. S. acutus and S. paradoxum exhibited higher accumulation tendency than the other two species. S. paradoxum showed the lowest As reduction rate (i.e., As(V) to As(III)) compared to other species, although, without significant variations. The morphological changes were observed in phytoplankton cells in response to increased salinity stress. Moreover, As(V) concentrations in the culture medium significantly decreased by day 7–14. Thus, this study presents a conceptual model of the As biotransformation pattern by axenic freshwater phytoplankton.

It is highly likely that the toxicity of water accommodated fractions (WAF) will influence marine microalgae, and consequently lead to potential risk for the marine ecological environment. However, it was often neglected whether WAF can influence the transformation of relative compounds in organisms. The metabolism of amino acids (AAs) can be used to track physiological changes in microalgae because amino acids are the basis of proteins and enzymes. In this study, using marine Chlorophyta Platymonas helgolandica as the test organism, the effects of different concentrations of WAF on AA compositions and stable carbon isotope ratios (δ¹³C) of individual AAs of Platymonas helgolandica were investigated. The results showed that the WAF of #180 fuel oil had an obvious suppressing effect on the growth and chlorophyll a content of microalgae. The growth inhibitory rate at 96 h was 80.66% at a WAF concentration of 0.50 mg L⁻¹ compared with the control. Furthermore, seven among the 16 AAs, including alanine, cysteine, proline, aspartic acid, lysine, histidine and tyrosine, had relatively high abundance. Under the glycolysis pathway, the cysteine abundance was higher than control, meaning that the biosynthesized pathway of alanine through cysteine as a precursor could be damaged. Phosphoenolpyruvate (PEP) was an important synthesis precursor of alanine (leucine) and aromatic AA family (Phenylalanine and tyrosine), and played an important role in δ¹³CAAₛ fractionation under the WAF stress. Under the TCA pathway, to protect cell metabolism activities under WAF stress, the δ¹³C value of threonine and proline abundance in microalgae with the increase in WAF stress. Therefore, δ¹³CAAₛ fractionation can be used as a novel method for toxicity evaluation of WAF on future.

Microalgae have become imperative for biological wastewater treatment. Its capability in biological purification of wastewaters from different origins while utilizing wastewater as the substrate for growth has manifest great potentials as a sustainable and economical wastewater treatment method. The wastewater grown microalgae have also been remarked in research to be a significant source of value-added bioproducts and biomaterial. This paper highlights the multifaceted roles of microalgae in wastewater treatment from the extent of microalgal bioremediation function to environmental amelioration with the involvement of microalgal biomass productivity and carbon dioxide fixation. Besides, the uptake mechanism of microalgae in wastewater treatment was discussed in detail with illustrations for a comprehensive understanding of the removal process of undesirable substances. The performance of different microalgae species in the uptake of various substances was studied and summarized in this review. The correlation of microalgal treatment efficacy with various algal strain types and the bioreactors harnessed for cultivation systems was also discussed. Studies on the alternatives to conventional wastewater treatment processes and the integration of microalgae with accordant wastewater treatment methods are presented. Current research on the biological and technical approaches for the modification of algae-based wastewater system and the maximization of biomass production is also reviewed and discussed. The last portion of the review is dedicated to the assertion of challenges and future perspectives on the development of microalgae-based wastewater treatment technology. This review serves as a useful and informative reference for readers regarding the multifaceted roles of microalgae in the application of wastewater biotreatment with detailed discussion on the uptake mechanism.