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Environmental impact of sunscreen nanomaterials: Ecotoxicity and genotoxicity of altered TiO₂ nanocomposites on Vicia faba
2011
Foltête, Anne-Sophie | Masfaraud, Jean-François | Bigorgne, Emilie | Nahmani, Johanne | Chaurand, Perrine | Botta, Céline | Labille, Jérôme | Rose, Jerome | Férard, Jean-François | Cotelle, Sylvie
Mineral sunscreen nanocomposites, based on a nano-TiO₂ core, coated with aluminium hydroxide and dimethicone films, were submitted to an artificial ageing process. The resulting Altered TiO₂ Nanocomposites (ATN) were then tested in the liquid phase on the plant model Vicia faba, which was exposed 48 h to three nominal concentrations: 5, 25 and 50 mg ATN/L. Plant growth, photosystem II maximum quantum yield, genotoxicity (micronucleus test) and phytochelatins levels showed no change compared to controls. Oxidative stress biomarkers remained unchanged in shoots while in roots, glutathione reductase activity decreased at 50 mg ATN/L and ascorbate peroxidase activity decreased for 5 and 25 mg ATN/L. Nevertheless, despite the weak response of biological endpoints, ICP-MS measurements revealed high Ti and Al concentrations in roots, and X-ray fluorescence micro-spectroscopy revealed titanium internalization in superficial root tissues. Eventual long-term effects on plants may occur.
Mostrar más [+] Menos [-]Atmospheric phenanthrene pollution modulates carbon allocation in red clover (Trifolium pratense L.)
2011
Desalme, Dorine | Binet, Philippe | Epron, Daniel | Bernard, Nadine | Gilbert, Daniel | Toussaint, Marie-Laure | Plain, Caroline | Chiapusio, Geneviève
The influence of atmospheric phenanthrene (PHE) exposure (160μgm⁻³) during one month on carbon allocation in clover was investigated by integrative (plant growth analysis) and instantaneous ¹³CO₂ pulse-labelling approaches. PHE exposure diminished plant growth parameters (relative growth rate and net assimilation rate) and disturbed photosynthesis (carbon assimilation rate and chlorophyll content), leading to a 25% decrease in clover biomass. The root-shoot ratio was significantly enhanced (from 0.32 to 0.44). Photosynthates were identically allocated to leaves while less allocated to stems and roots. PHE exposure had a significant overall effect on the ¹³C partitioning among clover organs as more carbon was retained in leaves at the expense of roots and stems. The findings indicate that PHE decreases root exudation or transfer to symbionts and in leaves, retains carbon in a non-structural form diverting photosynthates away from growth and respiration (emergence of an additional C loss process).
Mostrar más [+] Menos [-]Uptake and translocation of arsenite by Pteris vittata L.: Effects of glycerol, antimonite and silver
2011
Mathews, Shiny | Rathinasabapathi, Bala | Ma, Lena Q.
AsIII uptake in living cells is through aquaglyceroporin transporters, but it is unknown in arsenic-hyperaccumulator Pteris vittata. We investigated the effects of AsIII analogs glycerol and antimonite (SbIII) at 0–100 mM and aquaporin inhibitor AgNO₃ at 0–0.1 mM on the uptake of 0.1 mM AsIII or AsV by P. vittata over 1–2 h. Glycerol or SbIII didn’t impact AsIII or AsV uptake by P. vittata (p < 0.05), with As concentrations in the fronds and roots being 4.4–6.3 and 3.9–6.2 mg/kg. However, 0.01 mM AgNO₃ reduced As concentrations in the fronds and roots by 64% and 58%. Hence, AsIII uptake in P. vittata might be via an aquaporin transporter different from glycerol and SbIII transporters. Further as AsIII analogs and aquaporin inhibitor had no impact on AsV uptake, AsIII and AsV were likely taken up by different transporters in P. vittata. Our results imply a different AsIII transporter in P. vittata from other plants.
Mostrar más [+] Menos [-]Field controlled experiments of mercury accumulation in crops from air and soil
2011
Field open top chambers (OTCs) and soil mercury (Hg) enriched experiments were employed to study the influence of Hg concentrations in air and soil on the Hg accumulation in the organs of maize (Zea mays L.) and wheat (Triticum aestivum L.). Results showed that Hg concentrations in foliages were correlated significantly (p < 0.05) with air Hg concentrations but insignificantly correlated with soil Hg concentrations, indicating that Hg in crop foliages was mainly from air. Hg concentrations in roots were generally correlated with soil Hg concentrations (p < 0.05) but insignificantly correlated with air Hg concentrations, indicating that Hg in crop roots was mainly from soil. No significant correlations were found between Hg concentrations in stems and those in air and soil. However, Hg concentrations in upper stems were usually higher than those in bottom stems, implying air Hg might have stronger influence than soil Hg on stem Hg accumulation.
Mostrar más [+] Menos [-]The effect of mycorrhiza on the growth and elemental composition of Ni-hyperaccumulating plant Berkheya coddii Roessler
2011
Orłowska, Elżbieta | Przybyłowicz, Wojciech | Orlowski, Dariusz | Turnau, Katarzyna | Mesjasz-Przybyłowicz, Jolanta
The effect of arbuscular mycorrhizal fungi (AMF) on growth and element uptake by Ni-hyperaccumulating plant, Berkheya coddii, was studied. Plants were grown under laboratory conditions on ultramafic soil without or with the AM fungi of different origin. The AM colonization, especially with the indigenous strain, significantly enhanced plants growth and their survival. AMF affected also the elemental concentrations that were studied with Particle-induced X-ray emission (PIXE). AMF (i) increased K and Fe in shoots, Zn and Mn in roots, P and Ca both, in roots and shoots; (ii) decreased Mn in shoots, Co and Ni both, in shoots and roots. Due to higher biomass of mycorrhizal plants, total Ni content was up to 20 times higher in mycorrhizal plants compared to the non-mycorrhizal ones. The AMF enhancement of Ni uptake may therefore provide an improvement of a presently used technique of nickel phytomining.
Mostrar más [+] Menos [-]Behavior of decabromodiphenyl ether (BDE-209) in soil: Effects of rhizosphere and mycorrhizal colonization of ryegrass roots
2011
Wang, Sen | Zhang, Shuzhen | Huang, Honglin | Christie, Peter
A rhizobox experiment was conducted to investigate degradation of decabromodiphenyl ether (BDE-209) in the rhizosphere of ryegrass and the influence of root colonization with an arbuscular mycorrhizal (AM) fungus. BDE-209 dissipation in soil varied with its proximity to the roots and was enhanced by AM inoculation. A negative correlation (P < 0.001, R² = 0.66) was found between the residual BDE-209 concentration in soil and soil microbial biomass estimated as the total phospholipid fatty acids, suggesting a contribution of microbial degradation to BDE-209 dissipation. Twelve and twenty-four lower brominated PBDEs were detected in soil and plant samples, respectively, with a higher proportion of di- through hepta-BDE congeners in the plant tissues than in the soils, indicating the occurrence of BDE-209 debromination in the soil-plant system. AM inoculation increased the levels of lower brominated PBDEs in ryegrass. These results provide important information about the behavior of BDE-209 in the soil-plant system.
Mostrar más [+] Menos [-]Sensitivity of Bidens laevis L. to mutagenic compounds. Use of chromosomal aberrations as biomarkers of genotoxicity
2011
Pérez, D.J. | Lukaszewicz, G. | Menone, M.L. | Camadro, E.L.
The wetland macrophyte Bidens laevis possesses suitable cytological characteristics for genotoxicity testing. To test its sensitivity as compared to terrestrial plants species currently in use in standardized assays, Methyl Methanesulfonate (MMS), N-ethyl-N-nitrosourea (ENU) and Maleic Hydrazide (HM) were used. On the other hand, the insecticide Endosulfan (ES) – an environmentally relevant contaminant – was assayed in seeds and two-month old plants. Mitotic Index (MI), frequency of Chromosome Aberrations in Anaphase–Telophase (CAAT) and frequency of Abnormal Metaphases (AM) were analyzed. MH, MMS and ENU caused a significant decrease of the MI. MMS was aneugenic whereas MH and ENU were both aneugenic and clastogenic. ES caused a significant concentration-dependent increase of total- and aneugenic-CAAT in roots and a significant high frequency of AM at high concentrations. Because of its sensitivity to mutagenic substances, B. laevis can be regarded as a reliable and convenient species for genotoxicity assays especially if aquatic contaminants are evaluated.
Mostrar más [+] Menos [-]Plant uptake and dissipation of PBDEs in the soils of electronic waste recycling sites
2011
Huang, Honglin | Zhang, Shuzhen | Christie, Peter
Plant uptake and dissipation of weathered PBDEs in the soils of e-waste recycling sites were investigated in a greenhouse study. Eighteen PBDE congeners (tri- through deca-) were detected in the plant tissues. The proportion of lower brominated PBDEs (mono- through hexa-) in plant roots was higher than that in the soils. A concentration gradient was observed of PBDEs in plants with the highest concentrations in the roots followed by the stems and lowest in the leaves. Reduction rates of the total PBDEs in the soils ranged from 13.3 to 21.7% after plant harvest and lower brominated PBDEs were associated with a higher tendency to dissipate than the higher brominated PBDEs. This study provides the first evidence for plant uptake of weathered PBDEs in the soils of e-waste recycling sites and planting contributes to the removal of PBDEs in e-waste contaminated soils.
Mostrar más [+] Menos [-]Formation of iron plaque on mangrove roots receiving wastewater and its role in immobilization of wastewater-borne pollutants
2011
Pi, N. | Tam, N.F.Y. | Wong, M.H.
Iron (Fe) plaque formed on mangrove root increased with wastewater discharge, but the extent was species-specific. For Bruguiera gymnorrhiza, Fe plaque concentration was 0.80mgg⁻¹ root d.wt at Day 0 and increased to 4.59, 6.84 and 7.52mgg⁻¹ at Day 75 in the fresh water control (FW), synthetic wastewater with pollutant concentrations five times of municipal sewage (5SW) and double of 5SW (10SW) treatments, respectively; the respective increases in Excoecaria agallocha were from 0.70 to 2.37, 10.73 and 13.21mgg⁻¹. For Acanthus ilicifolius, similar increase was found in 5SW, but all of the plants were dead in 10SW at Day 75. The concentrations of heavy metals and phosphorus immobilized were positively correlated with the amounts of Fe plaque formed, but the regression coefficients varied among species. The performance of mangrove plants in wastewater treatments was related to the Fe plaque formed and its immobilized wastewater-borne pollutants.
Mostrar más [+] Menos [-]Temporal changes of polyphenols and enzyme activities in seedlings of Kandelia obovata under lead and manganese stresses
2011
Yan, Zhong Zheng | Tam, Nora Fung Yee
The temporal responses of peroxidase (POD), superoxide dismutase (SOD), total polyphenols (TP), extractable condensed tannins (ECT) to different levels of lead (Pb) or manganese (Mn) stress, as well as the metal accumulation, in seedlings of Kandelia obovata were investigated. Both stress time and stress intensity had significant effects with significant accumulation of Pb and Mn in roots at Day 1. Pb and Mn showed no significant effects on root or leaf TP and ECT at Day 1. Prolonged exposure to metals caused significant drops of root TP and ECT but increased in leaves at Days 7 and 49. POD activities decreased in both roots and leaves at Day 1, while POD and SOD both increased under moderate levels of Pb and Mn at Day 7. The present study showed that antioxidative enzyme activities were more sensitive indicators to Pb or Mn stresses than phenolic compounds.
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