Glyphosate-based herbicides (GBHs) are the most widely used pesticides for weed control. In parallel with the renewal of the active ingredient, polyethoxylated POE(15) containing GBHs were banned in the EU in 2016. Since then, co-formulants were changed and numerous GBHs are marketed with different excipients declared as inert substances. In our study, we focused to determine acute and chronic cytotoxicity (by Aliivibrio fischeri assay) and direct hormonal activity (estrogenic and androgenic effects measured by Saccharomyces cerevisiae BLYES/BLYAS strains, respectively) of glyphosate, AMPA, polyethoxylated POE(15) and 13 GBHs from which 11 formulations do not contain polyethoxylated POE(15). Among the pure substances, neither glyphosate nor AMPA had any effects, while polyethoxylated POE(15) exhibited pronounced toxicity and was also estrogenic but not androgenic. Regarding the acute and chronic cytotoxicity and hormonal activity of GBHs, dilution percentages calculated from EC₅₀ values were in the most cases by one or two order of magnitude lower than the minimum recommended dilution for agricultural and household use. Relation could not be observed between the biological effects and type of glyphosate-salts; hence toxicity could be linked to the co-formulants, which are not even declared in 3 GBHs. Toxicological evaluation must focus on these substances and free accessibility of GBHs should be reconsidered.

Glyphosate-based herbicides, the most extensively used herbicides in the world, are available in an enormous number of commercial formulations with varying additives and adjuvants. Here, we study the effects of one such formulation, Credit41, in two genetically diverse yeast strains. A quantitative trait loci (QTL) analysis between a sensitive laboratory strain and a resistant strain linked mitochondrial function to Credit41 resistance. Two genes encoding mitochondrial proteins identified through the QTL analysis were HFA1, a gene that encodes a mitochondrial acetyl CoA carboxylase, and AAC3, which encodes a mitochondrial inner membrane ATP/ADP translocator. Further analysis of previously studied whole-genome sequencing data showed that, although each strain uses varying routes to attain glyphosate resistance, most strains have duplications of mitochondrial genes. One of the most well-studied functions of the mitochondria is the assembly of Fe–S clusters. In the current study, the expression of iron transporters in the transcriptome increased in cells resistant to Credit41. The levels of iron within the cell also increased in cells exposed to Credit41 but not pure glyphosate. Hence, the additives in glyphosate-based herbicides have a significant contribution to the negative effects of these commercial formulations on biological systems.

Environment can be affected by a variety of micropollutants. In this paper, we develop a system to assess the toxicity on an environmental sample, based on the expression of a nanoluciferase under the control of the STB5 promotor in a yeast. The STB5 gene encodes for a transcription factor involved in a pleiotropic drug resistance and in the oxidative stress response. The response of the modified yeast was assessed using 42 micropollutants belonging to different families (antibiotics, pain killers, hormones, plasticizers, pesticides, etc.). Among them, 26 induced an increase of the bioluminescence for concentration ranges from pg.L⁻¹ to ng.L⁻¹. Surprisingly, for concentrations higher than 100 ng.L⁻¹, no response can be observed, suggesting that other mechanisms are involved when the stress increases. Analyzing the different responses obtained, we highlighted six nonmonotonic types of responses. The type of response seems to be independent of the properties of the compounds (polarity, toxicology, molecular weight) and of their family. In conclusion, we highlighted that a cellular response exists for very low exposition to environmental concentration of micropollutants and that it was necessary to explore the cellular mechanisms involved at very low concentration to provide a better risk assessment.

Pharmaceuticals and analogs of bisphenol A (BPA) are increasingly threatening environmental pollutants. In this study, mixtures of selected pharmaceuticals (diclofenac sodium salt, chloramphenicol, oxytetracycline hydrochloride, fluoxetine hydrochloride, estrone, ketoprofen, progesterone, gemfibrozil and androstenedione) were prepared with BPA and its two analogs (namely, bisphenols F and S) at such ratios to reflect environmentally detectable levels. Then, the mixture solutions were studied with a XenoScreen YES/YAS assay to determine the variations in the initial hormonal response of each pharmaceutical compound due to the presence of a bisphenol analog. The results obtained were modeled with the concentration addition (CA) and independent action (IA) approaches, the trueness of which was studied with model deviation ratios (MDR). The estrogenic agonistic activity of the drugs studied was most strongly affected by the presence of BPA in solution (twenty-one cases of synergy observed for CA models versus twelve cases of antagonism in the case of IA predictions). BPS shows a strong agonistic estrogenic impact on most of the drugs studied at medium and high concentration levels; androgenic agonistic activity was also impaired with elevated concentrations of BPS. Increasing the concentration of BPF in a reaction mixture also increased the number of YES + synergism incidences (for CA modeling). Estrone, progesterone and androstenedione were mostly affected by the highest BPF concentrations studied in the case of androgenic agonistic research performed.

Assessment of the impact of pharmaceutical residues on living organisms is a very complex subject. Apart from taking into account the toxicity of individual compounds, environmental factors should also be taken into account. In this paper, attempts were made to assess the impact of coexisting inorganic ions and changes in pH on the toxicity of ten selected pharmaceuticals. Two bioassays were used to measure the estrogenic and androgenic effects (XenoScreen YES/YAS – Saccharomyces cerevisiae) and acute toxicity (Microtox® – Vibrio fischeri).The Microtox® test gave the most definitive outputs concerning the determination of interaction type between drugs and chemical species. Synergism was proven for almost all drugs and chemical species, and only two cases of antagonism were found. Significant drug/pH interactions were rare.Regarding the XenoScreen YES/YAS bioassay, when estrogenic and androgenic agonistic effects (YES+ and YAS+, respectively) were studied, many cases of well-expressed synergism for all inorganic ions with limited number of drugs (diazepam, fluoxetine, estrone, chloramphenicol for the YES+ test and diazepam, progesterone, androstenedione, and estrone for the YAS+ test) were found. Antagonism was also proven for the YES+ test, especially for diclofenac and androstenedione interacting with cations. On the other hand, the YES- and YAS- tests (estrogenic and androgenic, respectively, antagonistic effects) did not indicate cases of synergetic interaction except for the couples Br−/diazepam and NH4+/ketoprofen. Antagonistic drug/ion interactions were detected only with diclofenac and fluoxetine. It is interesting that well-expressed (antagonism or synergism) drug/pH interactions were rare.Both tests were found utilizable in performing studies on impact of ions/pH fluctuations on drugs mixtures' toxicity confirming in most cases synergic impact of parameters studied on toxicity. The approach proposed in the paper seems to be proven as a reliable tool in assessing impact of abiotic factors on toxicity and endocrine potential of complex mixtures of pharmaceuticals' mixtures.

Several laccase-based products have been launched on the market over the years. However, there is little information about their toxicity and/or the toxicity of their residues. We evaluated the toxicity of a simulated denim-laundry wastewater containing Biolite BSN, a laccase-based product used for denim bleaching. The evaluation included a viability test with Saccharomyces cerevisiae, a phytotoxicity test with Sorghum vulgare, and a toxicity test with Daphnia magna. The viability of S. cerevisiae was reduced to 40%. The radicle and plumule growth of S. vulgare was reduced to 62 and 66%, respectively. The CL₅₀ for D. magna was 29.7%, which classified this water as toxic (according to the percent rank method). Biolite BSN was identified as the main cause of the toxic effects; furthermore, the chemical oxygen demand (COD) of the wastewater was significantly high (3346.2 mg/L), and more than 90% of the COD corresponded to Biolite BSN. The anaerobic biodegradability tests showed that the denim-laundry wastewater could be treated anaerobically; therefore, it was treated in an upflow anaerobic sludge blanket (UASB) reactor. The treatment reduced the toxicity and COD in 50 and 77%, respectively, and a methane yield of 311.1 mL CH₄/g of CODᵣₑₘₒᵥₑd was obtained. Based on these results, we recommend to analyze the toxicity of all textile chemicals, regardless of whether they are enzyme-based products. A UASB reactor can be used as the first treatment stage for similar effluents in order to reduce the COD and the toxicity and recover methane as an added benefit.

This article reports the deliverables of the experimental study on the production of a completely renewable biofuel from Manilkara zapota fruit and seed oil. It was attempted to synthesis ethyl ester from Manilkara zapota seed oil using bioethanol synthesized from decayed Manilkara zapota fruit. Bioethanol was produced through fermentation of decayed Manilkara zapota fruit, waste skin, and pulp with Saccharomyces cerevisiae and then distilled at 72°C. The bioethanol yield was noted as 10.45% (v/w). The 95.09% pure bioethanol and 4.9% water molecules were present in the distilled sample. Mechanically extracted raw Manilkara zapota seed oil was used for ethyl ester conversion. The molar ratio of bioethanol to oil, the quantity of KOH, and process temperature were investigated for the maximum yield of Manilkara zapota ethyl ester. A 9:1 molar ratio of bioethanol to oil, 1.5% (w/w) KOH, and 70°C process temperature were identified as enhanced ethanolysis process parameters. The maximum yield of ethyl ester was identified as 93.1%. Physicochemical characteristics of Manilkara zapota oil, bioethanol, and ethyl ester were measured as per the corresponding ASTM standards. It was found that both Manilkara Zapota ethyl ester and bioethanol synthesized from decayed Manilkara zapota fruit could be promising substitutes for fossil diesel and gasoline.

Textile industries use large amounts of water in dyeing processes and a wide variety of synthetic dyes. A small concentration of these dyes in the environment can generate highly visible pollution and changes in aquatic ecosystems. Adsorption, biosorption, and biodegradation are the most advantageous dye removal processes. Biodegradation occurs when enzymes produced by certain microorganisms are capable of breaking down the dye molecule. To increase the efficiency of these processes, cell immobilization enables the reuse of the immobilized cells and offers a high degree of mechanical strength, allowing metabolic processes to take place under adverse conditions. The aim of the present study was to investigate the use of Saccharomyces cerevisiae immobilized in activated sugarcane bagasse for the degradation of Acid Black 48 dye in aqueous solutions. For such, sugarcane bagasse was treated with polyethyleneimine (PEI). Concentrations of a 1 % S. cerevisiae suspension were evaluated to determine cell immobilization rates. Once immobilization was established, biodegradation assays for 240 h with free and immobilized yeast in PEI-treated sugarcane bagasse were evaluated by Fourier transform infrared spectrophotometry. The results indicated a probable change in the dye molecule and the possible formation of new metabolites. Thus, S. cerevisiae immobilized in sugarcane bagasse is very attractive for biodegradation processes in the treatment of textile effluents. © 2013 Springer Science+Business Media Dordrecht.

The release of heavy metals in aquatic systems due to the discharge of industrial wastewaters is a matter of environmental concern. Heat-inactivated cells of a flocculent strain of Saccharomyces cerevisiae were used in the bioremediation, in a batch mode, of a real electroplating effluent containing Cu, Ni, and Cr. In this approach, no previous reduction of Cr(VI) to Cr(III) was required. Cr(VI) was selectively removed (98%) by yeast biomass at pH 2.3. At this pH, Cr(VI) is mainly in the form of HCrO ₄ ⁻ and yeast surface is surrounded by H⁺ ions, which enhance the Cr(VI) interaction with biomass binding sites by electrostatic forces. Subsequently, pH of the effluent was raised up to 6.0; this pH maximizes the efficiency of cations removal since at this pH the main binding groups of yeast cells are totally or partially deprotonated. The passage of effluent through a series of sequential batches, at pH 6.0, allowed, after the third batch, the removal of Cu(II), Ni (II), Cr total, and Cr(VI) in the effluent to values below the legal limit of discharge. The strategy proposed in the present work can be used in plants for the treatment of heavy metals rich industrial effluents containing simultaneously Cr(VI) and Cr(III).

In this study, three promising yeast isolates were isolated from the sap of the Indian date palm tree (Phoenix sylvestris) and characterized by biochemical tests and 18S rRNA gene sequencing. They were confirmed as Saccharomyces cerevisiae and were designated as strains PYS-1, PYS-2, and PYS-3. These confirmed strains were used for the preparation of bioinoculants. Bioinoculant was prepared and applied to wheat crops, and the effect of Bioinoculant. Statistical analysis is carried out using analysis of variance (ANOVA), and it is found that the absorbance of chlorophyll, protein, and Indole Acetic Acid (IAA) content is significantly increased. The treatment of bioinoculant showed that crops significantly increased chlorophyll, protein, and IAA content. Further, we applied bioinoculant on the soil and measured the soil’s humus content before and after the treatment of bioinoculant. Then, a paired t-test was applied to check the effectiveness of the treatment, and it was found to significantly increase humus content in the soil. The use of bioinoculants is an economically feasible and eco-friendly method.