The genus Artemia sp. has been accepted as a reliable model organism for aquatic toxicity and nanotoxicity experiments, as far as the ISO TS 20787 has recently been published to standardize nanotoxicity test with this organism. Experimental and environmental conditions may affect the toxicity of nanomaterials on aquatic organisms including Artemia sp. nauplii. In this study, acute toxicity effects of silver nanoparticles (AgNPs) on the nauplii of Artemia salina was investigated under various conditions (e.g. different lights, salinities, temperatures, volume and agitation of exposure media and instar stages of nauplii). The EC values were calculated using Probit program and all data were analyzed statistically by SPSS software. At all test conditions, the immobilization rate of Artemia nauplii increased in a concentration-dependent manner (P < 0.05). The sensitivity of instar stage II to different concentrations of AgNPs was significantly higher than instar I (P < 0.05). The toxicity effect of AgNPs was affected by alteration of environmental conditions, so that the effective concentration (EC) values for instar I of A. salina decreased with increasing water temperature, decreasing water salinity and in continuous darkness condition. The EC50 value of AgNPs was significantly lower in 100 mL beakers (21.35 ± 5.67 mg L−1) than 10 mL well plates (42.44 ± 11.30 mg L−1). Agitation of exposure media did not affect the toxicity of AgNPs. The results indicated that the experimental and environmental conditions influence on the toxicity of AgNPs in the nauplii of A. salina.

Artificial surfaces for outdoor sporting grounds may pose environmental and health hazards that are difficult to assess due to their complex chemical composition. Ecotoxicity tests can indicate general hazardous impacts. We conducted growth inhibition (Pseudokirchneriella subcapitata) and acute toxicity tests (Daphnia magna) with leachates obtained from batch tests of granular infill material and column tests of complete sporting ground assemblies. Ethylene propylene diene monomer rubber (EPDM) leachate showed the highest effect on Daphnia magna (EC50 < 0.4% leachate) and the leachate of scrap tires made of styrene butadiene rubber (SBR) had the highest effect on P. subcapitata (EC10 = 4.2% leachate; EC50 = 15.6% leachate). We found no correlations between ecotoxicity potential of leachates and zinc and PAH concentrations. Leachates obtained from column tests revealed lower ecotoxicological potential. Leachates of column tests of complete assemblies may be used for a reliable risk assessment of artificial sporting grounds.

Streams impacted by acid mine drainage (AMD) typically present water exhibiting low pH and high metal concentrations. These factors result in the environmental degradation of watercourses. The objective of this study was to develop and evaluate an ecotoxicological screening tool (EST) to prioritise future remediation of streams impacted by AMD. The Bloubank stream drainage system in South Africa, served as study area for this purpose. In the initial EST development phase physicochemical variables were assessed while in the second phase, epilithic filamentous green algae biomass (chl-a mg m−2), diatoms and filamentous green algae community structures were employed as bioindicators as well as Daphnia magna toxicity assays. Using a weight of evidence approach, the first three sites receiving AMD were critically and seriously modified, followed by site 4 that was modified. Sites 1–3 with EST scores ≤70% were assessed as priority candidates for future restoration.

Using the marine rotifer Brachionus plicatilis acute toxicity tests, we estimated the toxicity of Corexit 9500A®, propylene glycol, and Macondo oil. Ratios of 1:10, 1:50 and 1:130 for Corexit 9500A®:Macondo oil mixture represent: maximum exposure concentrations, recommended ratios for deploying Corexit (1:10–1:50), 1:130 the actual dispersant:oil ratio used in the Deep Water Horizon spill. Corexit 9500A® and oil are similar in their toxicity. However, when Corexit 9500A® and oil are mixed, toxicity to B. manjavacas increases up to 52-fold. Extrapolating these results to the oil released by the Macondo well, suggests underestimation of increased toxicity from Corexit application. We found small differences in sensitivity among species of the B. plicatilis species complex, likely reflecting phylogenetic similarity. Just 2.6% of the water-accommodated fraction of oil inhibited rotifer cyst hatching by 50%, an ecologically significant result because rotifer cyst in sediments are critical resources for the recolonization of populations each Spring.

The free ion approach has been previously used to calculate critical limit concentrations for soil metals based on point estimates of toxicity. Here, the approach was applied to dose–response data for copper effects on seven biological endpoints in each of 19 European soils. The approach was applied using the concept of an effective dose, comprising a function of the concentrations of free copper and ‘protective’ major cations, including H+. A significant influence of H+ on the toxicity of Cu2+ was found, while the effects of other cations were inconsistent. The model could be generalised by forcing the effect of H+ and the slope of the dose–response relationship to be equal for all endpoints. This suggests the possibility of a general bioavailability model for copper effects on organisms. Furthermore, the possibility of such a model could be explored for other cationic metals such as nickel, zinc, cadmium and lead.

The discharge of oil well produced water (PW) provides a constant source of contaminants to the marine environment including polycyclic aromatic hydrocarbons, alkylated phenols, metals and production chemicals. High concentrations of PW cause adverse effects to exposed biota, including reduced survival, growth and reproduction. Here we explore the effects of PW on immune function in the blue mussel, Mytilus edulis. Mussels were exposed for 21 days to sublethal PW concentrations (0.125-0.5%) and cellular parameters were measured. Cell viability, phagocytosis and cytotoxicity were inhibited after exposure to 0.25% and 0.5% PW, whilst the 0.125% PW treatment produced significant increases in these biomarker responses. This biphasic response was only observed after 7 days exposure; longer exposure periods led to a reduction in immune parameters. Results indicate that PW concentrations close to the discharge point cause modulation to cellular immunity. The implications for longer-term disease resistance are discussed. Exposure to produced water alters immune function in the sentinel species Mytilus edulis.

The study of the effect of the sorption of linear alkylbenzene sulfonates (LAS) on the bioavailability to marine benthic organisms is essential to refine the environmental risk assessment of these compounds. According to the equilibrium partitioning theory (EqP), the effect concentration in water-only exposure will be similar to the effect concentration in the sediment pore water. In this work, sorption and desorption experiments with two marine sediments were carried out using the compound C12-2-LAS. The effect of the sediment sorption on the toxicity of benthic organisms was studied in water-only and in sediment bioassays with the marine mud shrimp Corophium volutator. In addition, three common spiking methods were tested for its application in the toxicity tests, as well as the stability of the surfactant during the water-only and sediment-water test duration. LC50 values obtained from water-only exposure showed a good correspondence with the pore water concentrations calculated from the sorption and desorption isotherms in the spiked sediments.

Although many studies have focused on trace metals accumulation, investigations of talitrid amphipods as biomarkers are rare. This study explores the possibility of using the solar orientation capacity of Talitrus saltator as a behavioural marker of exposure to two essential (Cu and Zn) and two non-essential (Cd and Hg) metals. LC50 analyses performed before the solar orientation tests showed that the 72 h LC50 for Hg was 0.02 ppm while the 96 h LC50 values for Cu, Cd and Zn were 13.28 ppm, 27.66 ppm, and 62.74 ppm, respectively. The presence of metals in seawater affects the solar orientation capacity of T. saltator in a concentration-dependent manner and according to the toxicity ranking of the metals (Hg > Cu > Cd > Zn). Therefore, the solar orientation capacity of T. saltator seems to be a promising behavioural marker for exposure to trace metals. Solar orientation capacity is a promising behavioural marker for exposure to trace metals in sandhoppers.

In this study, the influence of the co-existence of TiO2 nanoparticles on the speciation of arsenite [As(III)] was studied by observing its adsorption and valence changing. Moreover, the influence of TiO2 nanoparticles on the bioavailability of As(III) was examined by bioaccumulation test using carp (Cyprinus carpio). The results showed that TiO2 nanoparticles have a significant adsorption capacity for As (III). Equilibrium was established within 30 min, with about 30% of the initial As (III) being adsorbed onto TiO2 nanoparticles. Most of aqueous As (III) was oxidized to As(V) in the presence of TiO2 nanoparticles under sunlight. The carp accumulated considerably more As in the presence of TiO2 nanoparticles than in the absence of TiO2 nanoparticles, and after 25-day exposure, As concentration in carp increased by 44%. Accumulation of As in viscera, gills and muscle of the carp was significantly enhanced by the presence of TiO2 nanoparticles. The co-existence of TiO2 nanoparticles could change the speciation of arsenite by adsorption and photo-oxidation, and enhance its bioaccumulation to carp.