We exposed flour beetles (Tribolium castaneum) to a mixture of four poly aromatic hydrocarbons (PAHs). The experimental setup was chosen such that the emphasis was on assessing partial effects. We interpreted the effects of the mixture by a process-based model, with a threshold concentration for effects on survival. The behavior of the threshold concentration was one of the key features of this research. We showed that the threshold concentration is shared by toxicants with the same mode of action, which gives a mechanistic explanation for the observation that toxic effects in mixtures may occur in concentration ranges where the individual components do not show effects. Our approach gives reliable predictions of partial effects on survival and allows for a reduction of experimental effort in assessing effects of mixtures, extrapolations to other mixtures, other points in time, or in a wider perspective to other organisms. We show a mechanistic approach to assess effects of mixtures in low concentrations.

In the current study we have tested the application of Beauveria bassiana (Hypocreales: Cordycipitaceae) alone and in combination with fipronil at two doses against Tribolium castaneum (Coleoptera: Tenebrionidae), Rhyzopertha dominica (Coleoptera: Bostrychidae), Sitophilus granarius (Coleoptera: Curculionidae), and Trogoderma granarium (Coleoptera: Dermestidae) under laboratory and field conditions. At laboratory conditions, the combination of B. bassiana with the highest dose of fipronil produced the highest mortality. At different temperatures, mortality was increased with the increase in temperature. Maximum mortality was observed at 30 °C, followed by 25 °C and 20 °C for all tested species. Different treatments significantly reduced the progeny number in comparison to control groups for all tested species at all temperatures. In the persistence trial, all treatments that included the combinations of B. bassiana with fipronil produced significantly higher mortalities than the single treatments for all tested species over a period of 6 months. Furthermore, all treatments significantly reduced the number of progenies of all insect species in comparison with the control groups over the same storage period. In field trials, mortalities of all tested insect species were significantly higher on wheat treated with B. bassiana, fipronil, or their combinations than on controls for an entire storage period of 180 days. Overall, R. dominica was found the most susceptible species followed by S. granarius, T. castaneum, and T. granarium. The findings of the current study suggest that the use of B. bassiana and fipronil as grain protectants may provide elevated control against major stored-grain insect species during a prolonged period of storage.

In the present study, we evaluated the insecticidal efficacy of diatomaceous earth (DE) and pirimiphos-methyl for the control of phosphine-susceptible and phosphine-resistant populations of Tribolium castaneum (Herbst) and Sitophilus oryzae (L.). Insecticides were applied on wheat or rice at two doses: DE was applied at 1000 and 2000 ppm and pirimiphos-methyl at 1 and 5 ppm. Adult mortality was measured after 7, 14, and 21 days of exposure, and progeny production capacity on the treated substrates was evaluated 65 days later. For T. castaneum, we found that DE, at 2000 ppm, was able to provide 100% control of two of the three populations tested, while for the third population mortality reached only 84%. Similarly, there were differences in mortality levels after exposure to DE-treated grains between the two S. oryzae populations tested. At 1 ppm, pirimiphos-methyl was not effective for any of the T. castaneum populations tested, but complete mortality was recorded for all populations at 5 ppm. In general, populations of S. oryzae were more susceptible than those of T. castaneum, for both commodities. Our data indicate that both insecticides can be used with success in phosphine resistance management programs, but there are populations of a given species that may be less susceptible, which constitutes a preliminary screening essential.

The residual efficacy of the enhanced diatomaceous earth (DE) formulation DEBBM alone and in combination with Beauveria bassiana (Hypocreales: Cordycipitaceae) or with the neonicotinoid insecticide imidacloprid against Tribolium castaneum (Coleoptera: Tenebrionidae), Rhyzopertha dominica (Coleoptera: Bostrychidae), Cryptolestes ferrugineus (Coleoptera: Laemophloeidae), and Liposcelis paeta (Psocoptera: Liposcelididae) was investigated in the laboratory. The combination treatments were more effective compared to the single treatments against all examined species. The combinations of DEBBM and imidacloprid and imidacloprid with the highest dose rate of B. bassiana provided the highest mortality values against all tested species for 90 days of storage period. The combination of DEBBM plus B. bassiana resulted to the highest mortalities and to the lowest offspring production of all combinations tested after 180 days of storage. Mortality of adults for each test insect species was decreased over the storage period of 6 months, and the progeny production was increased with the extended storage period. Among the tested insect species, L. paeta was the most susceptible to all three grain protectants followed by C. ferrugineus, R. domina, and T. castaneum. The findings of the current study suggest that the use of DEBBM, imidacloprid, and B. bassiana as grain protectants may provide elevated control of major stored-grain insect species during a prolonged period of storage.

The objective of this work was to analyze four essential oils (EOs) from Rhododendron species in China and evaluate their repellent activity against Tribolium castaneum, Lasioderma serricorne, and Liposcelis bostrychophila adults. These four EOs from Rhododendron species, including R. capitatum, R. przewalskii, R. mucronulatum, and R. micranthum, were obtained by hydrodistillation. Major components of four EOs were identified as sesquiterpenoids by GC-MS. The relatively high components included cedrene (22.20%), borneol (36.64%), 4-(2,3,4,6-tetramethylphenyl)-3-buten-2-one (27.74%), and germacrene D (27.60%). Repellent activity of EOs from Rhododendron species was investigated against T. castaneum, L. serricorne, and L. bostrychophila adults for the first time. In this study, EOs had demonstrated their repellent activities against three stored-product insects in 2- and 4-h exposure. The above results can not only provide comprehensive utilization of plant resources of Rhododendron genus but also establish a very good perspective of novel application to control stored-product insects.

The effectiveness of Grain-Guard, an improved form of diatomaceous earth (DE), with low risk to the environment, was evaluated against the storage pests Liposcelis paeta, Cryptolestes ferrugineus, Rhyzopertha dominica, and Tribolium castaneum on four grain commodities, wheat, rice, maize and sorghum. The overall mortality of stored grain pests increased with the rise of application rate and exposure intervals of diatomaceous earth and decreased over 120 days of post-treatment period. Our results revealed that mortality of adults 14 days post-disclosure was > 80% on wheat at the start of post-treatment than rice, maize, and sorghum, respectively. During the first 60 days of post-treatment, adult mortality increased, whereas later on, a steady decrease in adult mortality was observed. Considerable differences in dose rates were observed on mortality levels along with grain commodities. Following 14 days of exposure, all adults of four species were dead on wheat at 100 ppm and on rice at 150 ppm except maize and sorghum. The suppression of progeny was noticeably higher at the beginning of post-treatment duration while after 60-days of post-treatment, progeny numbers started to increase with the rise in post-treatment durations. This new improved DE formulation was found to be effective at dose rates that are extensively lower than required with previous DE formulations and will contribute to lower the risk of health and environment.

Insecticidal treatment is a common practice for the control of stored-product insect pests. Most studies are focused on the direct effects of insecticides on target insects, while there are no data on the indirect effects on adults in terms of progeny fitness. This study deals with the effect of pirimiphos-methyl on adults of Tribolium castaneum (Coleoptera: Tenebrionidae), investigating their exposure time cost to progeny fitness. For this purpose, females of T. castaneum were exposed for 1, 3, 8, 16, 24, and 72 h to pirimiphos-methyl and progeny birth or death rates were calculated. The fecundity, as well as the survival of progeny, was affected by the parental exposure to the insecticide. When females were exposed for 1 and 3 h to pirimiphos-methyl, the net reproductive rate of the progeny was 4.1 and 4.3 females/female, respectively, which did not statistically differ with the progeny in control treatment (6.3 females/female). Further exposure time to pirimiphos-methyl affected significantly the progeny net reproductive rate, where the lowest value was observed at the 72-h exposure time (0.97 females/female). The intrinsic rate of increase, as well as the finite rate of increase, did not differ between the progeny of the control treatment (0.029 females/female/day and 1.029, respectively) and the progeny of exposed females at 1 h (0.021 females/female/day and 1.021, respectively) or 3 h (0.023 females/female/day and 1.023, respectively). By increasing the time of exposure to pirimiphos-methyl, a detrimental effect to progeny fitness was revealed based on the values of the intrinsic and finite rate of increase. The lowest values of these parameters were observed at the 72-h exposure time (0.001 females/female/day and 1.000, respectively). The same trend was observed in the values of the doubling time. In contrast, the mean generation time did not statistically differ between control and all insecticide treatments. The fact that the exposure of parental females of T. castaneum to pirimiphos-methyl negatively affects the demographic parameters of their offspring production should be seriously taken into account when management strategies are applied against this notorious stored-product insect species since it could reduce the repeatedly insecticidal applications in storage facilities.

Essential oils (EOs) extracted from leaves (EL) and fruit pericarp (EFP) of Zanthoxylum planispinum var. dintanensis were analyzed for their chemical composition by GC-MS technique and evaluated for their fumigant, contact toxicity and repellency against three stored-product insects, namely Tribolium castaneum, Lasioderma serricorne, and Liposcelis bostrychophila adults. Results of GC-MS analysis manifested that EL and EFP of Z. planispinum var. dintanensis were mainly composed of oxygenated monoterpenes. Major components included linalool, sylvestrene and terpinen-4-ol. The obvious variation observed between two oil samples was that EL contained 2-dodecanone (11.52%) in addition to the above mentioned components, while this constituent was not detected in EFP. Bioassays of insecticidal and repellent activities were performed for EL, EFP as well as some of their individual compounds (linalool, terpinen-4-ol and 2-dodecanone). Testing results indicated that EL, EFP, linalool, terpinen-4-ol and 2-dodecanone exhibited potent insecticidal and repellent activities against the three target insects selected. Among the three individual compounds, 2-dodecanone was significantly toxic to T. castaneum (LD₅₀ = 5.21 μg/adult), L. serricorne (LD₅₀ = 2.54 μg/adult) and L. bostrychophila (LD₅₀ = 23.41 μg/cm²) in contact assays and had beneficial repellent effects on L. serricorne at 2 and 4 h post-exposure. The anti-insect efficacy of Z. planispinum var. dintanensis EO suggests it has potential to be used as botanical insecticide or repellent to control pest damage in warehouses and grain stores.

The natural and ecologically safe control of stored product insects has gained considerable attention in modern society. In this study of further searching for botanical pesticides from wild-growing plant, the contact toxicity and repellency towards Tribolium castaneum and Liposcelis bostrychophila were assessed for the essential oil (EO) from Ostericum viridiflorum. The EO was distilled from aboveground parts of O. viridiflorum and checked by gas chromatography-mass spectrometry. Twenty-two compounds were identified and the main components were β-caryophyllene (24.3%), α-humulene (21.0%), apiol (10.2%), and carotol (2.5%). For bioactivity tests, results indicated that the EO and its two main compounds (β-caryophyllene and α-humulene) all showed potent contact toxicity towards L. bostrychophila with LD₅₀ values of 44.52 μg/cm², 74.11 μg/cm², and 118.56 μg/cm², respectively. The EO and the two main compounds also exhibited comparable repellency towards T. castaneum and L. bostrychophila. The results evidenced the EO of O. viridiflorum aboveground parts and its major compounds could be considered for the development of eco-friendly botanical insecticides and repellents in controlling stored product insects.

The main objectives of the present study are to introduce new, ecologically safe, and natural compounds for controlling red flour beetle, Tribolium castaneum, and to understand the possible mode of action of these compounds. Therefore, the insecticidal and repellent activities of two phenylpropenes and six monoterpenes have been evaluated against the adults of T. castaneum. The inhibitory effects of these compounds on the activity of adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) were also tested. In fumigant toxicity assay, (−)-terpinen-4-ol (LC₅₀ = 20.47 μl/l air) and α-terpinene (LC₅₀ = 23.70 μl/l air) exhibited the highest toxicity without significant differences between them. Moreover, (−)-menthone and p-cymene showed strong toxicity, while (−)-citronellal, trans-cinnamaldehde, and eugenol were not active. In contact toxicity assay, the two phenylpropenes, trans-cinnamaldehde and eugenol, had the highest toxicity with same LC₅₀ value of 0.02 mg/cm². The monoterpenes and phenylpropenes showed pronounced repellent effect on the adults of T. castaneum at 0.001 mg/cm² with (−)-menthone, trans-cinnamaldehyde, and α-terpinene being the most effective after 2 h of exposure. Repellent activity depended on compound, exposure time, and concentration. On the other hand, the tested compounds exhibited strong inhibition of ATPases form the larvae of T. castaneum as their IC₅₀ values ranged between 1.74 and 19.99 mM. In addition, (−)-citronellal (IC₅₀ = 9.82 mM) and trans-cinnamaldehde (IC₅₀ = 23.93 mM) caused the highest inhibitory effect on AChE, while α-pinene (IC₅₀ = 53.86) and p-cymene (IC₅₀ = 68.97 mM) showed the weakest inhibitory effect. The results indicated that the tested phenylpropenes and monoterpenes had the potential to be developed as natural insecticides and repellents for controlling T. castaneum.