Organisms that rely on aquatic habitats in roaded landscapes face a growing array of consequences from pollution, especially due to freshwater salinization. Critically, these consequences can vary from population to population depending on exposure histories and evolutionary responses. Prior studies using transplant and common garden experiments have found that aquatic-stage wood frogs (Rana sylvatica) from roadside populations are less fit in the wild and more sensitive to road salt than their counterparts from woodland populations away from roads. While this pattern is consistent with local maladaptation, unresolved insights into the timing and duration of these effects leave open the possibility that negative outcomes are countered during development. Here, we asked whether the survival disadvantage of roadside wood frogs is stage-specific, and whether this disadvantage reverses before metamorphosis. We used a common garden road salt exposure experiment and a field-based reciprocal transplant experiment to examine differences in survival across life-history stage and with respect to population type. In each experimental context, roadside embryos showed a survival disadvantage relative to woodland embryos, and this disadvantage was not reversed prior to metamorphosis. We also found that salt exposure delayed metamorphosis more strongly for roadside than woodland populations. Together, these results suggest that local maladaptation in aquatic-stage wood frogs is driven by embryonic sensitivity to salt and that roadside populations are further compromised by delayed developmental rates. Future studies should consider which embryonic traits fail to adapt to salt toxicity, and how those traits might correlate with terrestrial trait variation.

PM₂.₅ pollution is caused by multiple factors and determining how these factors affect PM₂.₅ pollution is important for haze control. In this study, we modified the geographically weighted regression (GWR) model and investigated the relationships between PM₂.₅ and its influencing factors. Experiments covering 368 cities and 9 urban agglomerations were conducted in China in 2015 and more than 20 factors were considered. The modified GWR coefficients (MGCs) were calculated for six variables, including two emission factors (SO₂ and NO₂ concentrations), two meteorological factors (relative humidity and lifted index), and two topographical factors (woodland percentage and elevation). Then the spatial distribution of MGCs was analyzed at city, cluster, and region scales. Results showed that the relationships between PM₂.₅ and the different factors varied with location. SO₂ emission positively affected PM₂.₅, and the impact was the strongest in the Beijing–Tianjin–Hebei (BTH) region. The impact of NO₂ was generally smaller than that of SO₂ and could be important in coastal areas. The impact of meteorological factors on PM₂.₅ was complicated in terms of spatial variations, with relative humidity and lifted index exerting a strong positive impact on PM₂.₅ in Pearl River Delta and Central China, respectively. Woodland percentage mainly influenced PM₂.₅ in regions of or near deserts, and elevation was important in BTH and Sichuan. The findings of this study can improve our understanding of haze formation and provide useful information for policy-making.

Critical levels (CLEs) of atmospheric ammonia based on biodiversity changes have been mostly calculated using small-scale single-source approaches, to avoid interference by other factors, which also influence biodiversity. Thus, it is questionable whether these CLEs are valid at larger spatial scales, in a multi- disturbances context. To test so, we sampled lichen diversity and ammonia at 80 sites across a region with a complex land-cover including industrial and urban areas. At a regional scale, confounding factors such as industrial pollutants prevailed, masking the CLEs. We propose and use a new tool to calculate CLEs by stratifying ammonia concentrations into classes, and focusing on the highest diversity values. Based on the significant correlations between ammonia and biodiversity, we found the CLE of ammonia for Mediterranean evergreen woodlands to be 0.69 μg m−3, below the previously accepted value of 1.9 μg m−3, and below the currently accepted pan-European CLE of 1.0 μg m−3.

It is of great significance to explore the remediation pattern in actual heavy metal (HM) contaminated sites. The field trial was carried out to research the remediation effect of biochar near a lead-zinc smelter in Feng County, China, under the rotation condition of different crops. This kind of cultivation mode is very representative in northern of China. And the pattern of production and restoration is suitable for scarce land resources and large food demand in China. The changes of soil physiochemical properties with the biochar addition, crop growth and the accumulated HMs by crops were focused on. The results showed the biochar application was excellent in improving soil nutrient elements and crop growth. The contents of TK were more obvious than those of TN and TP, with an increase of 2.6%–28.2% compared with the controls (without the addition of biochar). The yield of first season crops, i.e., soybean and corn, increased by 30%–42% and 34%–61%, respectively, and the second season crops, i.e., rape and wheat, with the increment of 25%–41% and 9%–29%, respectively. The availability forms of Cd and Pb decreased by 1.07–10.0% and 2.92–8.35%, respectively. While the improvement on the status of the HMs accumulated by crops was disappointing. The contents of HMs accumulated by crops increased to varying degrees (e.g., Pb and Zn in root, Cu and Pb in grain, and Cd in stems and leaves). Moreover, the concentrations of HMs in seeds of crops were higher than the limited levels given by the Chinese directive. Considering the results of the study and food safety, it is suggested to change the nature of the land around the smelter into woodland or construction land to prohibit the cultivation of food crops in this area.

Artificial light at night (ALAN) is a recognised disruptor of biological function and ecological communities. Despite increasing research effort, we know little regarding the effect of ALAN on woody plants, including trees, or its indirect effects on their colonising invertebrates. These effects have the potential to disrupt woodland food webs by decreasing the productivity of invertebrates and their secretions, including honeydew and lerps, with cascading effects on other fauna. Here, we cultivated juvenile river red gums (Eucalyptus camaldulensis) for 40 weeks under experimentally manipulated light (ALAN) or naturally dark (control) conditions. To assess direct impacts on tree growth, we took multiple measures of growth at four time periods, and also measured physiological function, biomass and investment in semi-mature trees. To assess experimentally the direct and indirect (tree-mediated) impacts of ALAN on invertebrates, from 19 weeks onwards, we matched and mismatched trees with their original ALAN environments. We colonised trees with a common herbivore of E. camaldulensis, the red gum lerp psyllid (Glycaspis nr. brimblecombei) and then measured the effects of current and historic tree lighting treatment on the psyllid life cycle. Our data revealed direct effects of ALAN on tree morphology: E. camaldulensis trees exposed to ALAN shifted biomass allocation away from roots and into leaves and increased specific leaf area. However, while the intensity of ALAN was sufficient to promote photosynthesis (net carbon gain) at night, this did not translate into variation in tree water status or photosystem adaptation to dim night-time light for ALAN-exposed trees. We found some evidence that ALAN had broad-scale community effects—psyllid nymphs colonising ALAN trees produced more lerps—but we found no other direct or indirect impacts of ALAN on the psyllid life cycle. Our results suggest that trees exposed to ALAN may share morphological responses with trees under dim daylight conditions. Further, ALAN may have significant ‘bottom-up’ effects on Eucalyptus woodland food webs through both trees and herbivores, which may impact higher trophic levels including woodland birds, mammals and invertebrates.

Land-use types may affect soil aggregates' stability and organic carbon (OC) distribution characteristics, but little is known about the effects on the distribution characteristics of microplastics (MPs) in the aggregates. Hence, the MPs abundance of soil aggregates and analyzed aggregates’ stability, composition, and OC content from two soil layers of four land-use types in Gansu Province were investigated in this study. The total MPs abundances in woodland, farmland (wheat, maize, and potato), orchard, and intercropping (potato + apple orchard) of top and deep soils were 1383.3 and 1477.9, 1324.6 and 931.1, 1757.1 and 1930.9, 2127.2 and 1998.0, 1335.9 and 886.7, and 1777.5 and 1683.3 items kg⁻¹, respectively. The largest MPs abundance was detected in the >5 mm fractions of topsoil in potato (3077.3 items kg⁻¹), followed by maize (3044.7 items kg⁻¹) and intercropping (2718.4 items kg⁻¹). In the topsoil, the total MPs abundance increased significantly with decreasing aggregate stability, and also was positively correlated with bulk density, microbial biomass, and total nitrogen contents of bulk soil. Summarizing, the abundance distribution of MPs correlates with the soil aggregate characteristics of the different land-use types.

The effects of atmospheric pollution on plant species richness (nₛₚ) are of widespread concern. We carried out a modelling exercise to estimate how nₛₚ in British semi-natural ecosystems responded to atmospheric deposition of nitrogen (Ndₑₚ) and sulphur (Sdₑₚ) between 1800 and 2010. We derived a simple four-parameter equation relating nₛₚ to measured soil pH, and to net primary productivity (NPP), calculated with the N14CP ecosystem model. Parameters were estimated from a large data set (n = 1156) of species richness in four vegetation classes, unimproved grassland, dwarf shrub heath, peatland, and broadleaved woodland, obtained in 2007. The equation performed reasonably well in comparisons with independent observations of nₛₚ. We used the equation, in combination with modelled estimates of NPP (from N14CP) and soil pH (from the CHUM-AM hydrochemical model), to calculate changes in average nₛₚ over time at seven sites across Britain, assuming that variations in nₛₚ were due only to variations in atmospheric deposition. At two of the sites, two vegetation classes were present, making a total of nine site/vegetation combinations. In four cases, nₛₚ was affected about equally by pH and NPP, while in another four the effect of pH was dominant. The ninth site, a chalk grassland, was affected only by NPP, since soil pH was assumed constant. Our analysis suggests that the combination of increased NPP, due to fertilization by Ndₑₚ, and decreased soil pH, primarily due to Sdₑₚ, caused an average species loss of 39% (range 23–100%) between 1800 and the late 20th Century. The modelling suggests that in recent years nₛₚ has begun to increase, almost entirely due to reductions in Sdₑₚ and consequent increases in soil pH, but there are also indications of recent slight recovery from the eutrophying effects of Ndₑₚ.

The change in land-use from woodland to crop production leads to increased nitrous oxide (N2O) emissions. An understanding of the main N2O sources in soils under a particular land can be a useful tool in developing mitigation strategies. To better understand the effect of land-use on N2O emissions, soils were collected from 5 different land-uses in southeast China: shrub land (SB), eucalyptus plantation (ET), sweet potato farmland (SP), citrus orchard (CO) and vegetable growing farmland (VE). A stable isotope experiment was conducted incubating soils from the different land use types at 60% water holding capacity (WHC), using 15NH4NO3 and NH415NO3 to determine the dominant N2O production pathway for the different land-uses. The average N2O emission rates for VE, CO and SP were 5.30, 4.23 and 3.36 μg N kg−1 dry soil d−1, greater than for SB and ET at 0.98 and 1.10 μg N kg−1 dry soil d−1, respectively. N2O production was dominated by heterotrophic nitrification for SB and ET, accounting for 51 and 50% of N2O emissions, respectively. However, heterotrophic nitrification was negligible (<8%) in SP, CO and VE, where autotrophic nitrification was a primary driver of N2O production, accounting for 44, 45 and 66% for SP, CO and VE, respectively. Denitrification was also an important pathway of N2O production across all land-uses, accounting for 35, 35, 49, 52 and 32% for SB, ET, SP, CO and VE respectively. Average N2O emission rates via autotrophic nitrification, denitrification and heterotrophic nitrification increased significantly with gross nitrification rates, NO3− contents and C:N ratios respectively, indicating that these were important factors in the N2O production pathways for these soils. These results contribute to our understanding and ability to predict N2O emissions from different land-uses in subtropical acidic soils and in developing potential mitigation strategies.

A key criterion of the UK Government's policy on sustainable forest management is safeguarding the quality and quantity of water. Forests and forestry management practices can have profound effects on the freshwater environment. Poor forest planning or management can severely damage water resources at great cost to other water users; in contrast good management that restores and maintains the natural functions of woodland can benefit the whole aquatic ecosystem.Forests and forest management practices can affect surface water acidification. Monitoring of water chemistry in ten forest and two moorland acid-sensitive catchments in upland Wales commenced in 1991. The streams were selected to supplement the United Kingdom Upland Waters Monitoring Network (UWMN) with additional examples of afforested catchments. Analysis of 22 years of water chemistry data revealed trends indicative of recovery from acidification. Excess sulphate exhibited a significant coherent decline, accompanied by increases in pH and “charge-balance based” acid neutralising capacity (CB-ANC). Alkalinity and “alkalinity-based” acid neutralising capacity (AB-ANC) exhibited fewer trends, possibily due to the variable responses of the organic - carbonate species to increasing pH in these low alkalinity streams. Whilst total anthropogenic acidity declined, dissolved organic carbon and Nitrate-Nitrogen (NNO₃) concentrations have risen, and the contribution of NNO₃ to acidification has increased.Between-stream variability was analysed using Principal Component Analysis of the trend slopes. Hierarchical clustering of the changes in stream water chemistry indicated three distinct clusters with no absolute distinction between moorland and forest streams. Redundancy analysis was used to test for significant site-specific variables that explained differences in the trend slopes, with rainfall, crop age, base cation concentration and forest cover being significant explanatory variables.