International audience | Organic matter is a major metal-retaining constituent in soils. Among the diversity of organic components in soils, particulate organic matter (POM) accumulates large amounts of metals, but the fate of such metal-associated POM is unknown. We studied different POM size fractions and their corresponding mineral size-fractions isolated from the surface horizon of a soil affected by metallurgical fallout. Analyses of total and EDTA extractible metal contents performed on all size fractions demonstrated that with decreasing POM size, larger metal concentrations were observed but they were less extractable. Micromorphological study revealed the occurrence of opaque parts in decaying POM fragments and their individualization as fine, irregularly shaped opaque fragments in the soil matrix. This work suggested a mutual sequestration of metal pollutants and organic carbon as micro-meter sized, metal-enriched organic particles derived from POM, representing an original pathway for natural attenuation of risk related to metal contaminated soils. (c) 2007 Elsevier Ltd. All rights reserved.

International audience | Phytoremediation is an emerging strategy to remediate soils contaminated with pollutants like explosives in which plants will uptake, degrade and/or accumulate pollutants. To implement this technology on a site contaminated with RDX, we chose rice, which is able to grow in lagoons, and we tested its ability to grow in soils with high levels of RDX and to decrease RDX concentrations in soil. Rice was grown for 40 days in soil contaminated with increasing [14C]RDX concentrations. Emergence and growth were not affected by RDX. Total chlorophyll content decreased with RDX concentrations of over 500 mg kg−1. Amounts of chlorophyll were correlated with the appearance of necrosis in leaf extremities. After 40 days, rice translocated 89% of uptaken radioactivity to leaves with 90% in leaf extremities. Analyzes of leaf extracts showed that 95% of radioactivity was RDX in its parent form. Necrosis appears to be a phytotoxic symptom of RDX accumulation.

[Departement_IRSTEA]MA [TR1_IRSTEA]TED / EPURE | International audience | Samples of pig slurry, as well as dairy and poultry manures collected in Brittany (western France)were analysed to test the ability of sterol profiles to provide a fingerprint of pig slurry contribution to soil organic matter. The data show that the 5β-stanol, known as coprostanol, is the most abundant sterol present in pig slurry, whereas this compound occurs only in minor amounts in both poultry and dairy manures. Moreover, systematic variations of (campesterol + sitosterol)/cholesterol (i.e., C28+29/C27) and (coprostanol + epi-coprostanol)/cholesterol (i.e., 5β/C27) ratios allow to discriminate clearly pig slurry from poultry and dairy manures. The robustness of the pig slurry sterol fingerprint was tested by analysing the sterol profiles of soil samples from an experimental field that had received a massive pig slurry input between 10 to 14 years ago. The results indicate that the specific sterol profile of pig slurry is conservative once the slurry has been incorporated into the soil. In particular, the diagnostic 5β/C27 ratio proves to be constant with time in soils having received pig slurry application, even 10 years after the end of the application. The sterol fingerprint of pig slurry is thus sufficiently distinctive from dairy and poultry manures, and also sufficiently time-resistant, to be of diagnostic value in determining whether a soil sample was once contaminated by pig slurry.

Recent EU legislation is directed to reverse the upward trends in the concentrations of agricultural pollutants in groundwater. However, uncertainty of the groundwater travel time towards the screens of the groundwater quality monitoring networks complicates the demonstration of trend reversal. We investigated whether trend reversal can be demonstrated by relating concentrations of pollutants in groundwater to the time of recharge, instead of the time of sampling. To do so, we used the travel time to monitoring screens in sandy agricultural areas in the Netherlands, determined by 3H/3He groundwater dating. We observed that concentrations of conservative pollutants increased in groundwater recharged before 1985 and decreased after 1990. Thereby, we demonstrated trend reversal of groundwater quality. From this research we concluded that 3H/3He dating can be used to facilitate (re)interpretation of existing groundwater quality data. The presented approach is widely applicable in areas with unconsolidated granular aquifers and large agricultural pressures on groundwater resources. Groundwater age dating reveals trends and trend reversal in groundwater quality.

This study estimates the effect of environmental parameters on the mobility of four inorganic contaminants (As, Zn, Pb and Cd) in soils from three areas in the Ebro and Meuse River basins, within the context of global change. An experimental method, applicable to various soil systems, is used to measure the effect of four global-change-sensitive parameters (temperature, gas phase composition, pH and microbial activity). The aqueous phase of batch incubations was sampled regularly to monitor toxic element concentrations in water. Statistical processing enabled discrimination of the most relevant variations in dissolved concentrations measured at different incubation times and under different experimental conditions. Gas phase composition was identified as the most sensitive parameter for toxic element solubilization. This study confirms that total soil concentrations of inorganic pollutants are irrelevant when assessing the hazard for ecosystems or water resource quality. An experimental method applicable for different soil systems enables the determination of the effect of environmental parameters, potentially affected by global change, on the mobilization of inorganic pollutants.

As part of the Endis-Risks project, the current study describes the occurrence of the chlorotriazine pesticides atrazine, simazine and terbutylazine in water, sediment and suspended matter in the Scheldt estuary (B-Nl) from 2002 to 2005 (3 samplings a year, 8 sampling points). Atrazine was found at the highest concentrations, varying from 10 to 736 ng/l in water and from 5 up to 10 ng/g in suspended matter. Simazine and terbutylazine were detected at lower concentrations. Traces of the targeted pesticides were also detected in sediments, but these were below the limit of quantification. As part of an ecotoxicological assessment, we studied the potential effect of atrazine on molting of Neomysis integer (Crustacea:Mysidacea), a resident invertebrate of the Scheldt Estuary and a proposed test organism for the evaluation of endocrine disruption. Following chronic exposure (3 weeks), atrazine did not significantly affect mysid molting at environmentally relevant concentrations (up to 1 μg/l). The water of the Scheldt estuary and its associated suspended solids are contaminated with chlorotriazines at concentrations that do not affect mysid molting.

Photosynthetic stimulation and stomatal conductance (Gs) depression in Quercus ilex leaves at a CO2 spring suggested no down-regulation. The insensitivity of Gs to a CO2 increase (from ambient 1500 to 2000 μmol mol-1) suggested stomatal acclimation. Both responses are likely adaptations to the special environment of CO2 springs. At the CO2-enriched site, not at the control site, photosynthesis decreased 9% in leaves exposed to 2x ambient O3 concentrations in branch enclosures, compared to controls in charcoal-filtered air. The stomatal density reduction at high CO2 was one-third lower than the concomitant Gs reduction, so that the O3 uptake per single stoma was lower than at ambient CO2. No significant variation in monoterpene emission was measured. Higher trichome and mesophyll density were recorded at the CO2-enriched site, accounting for lower O3 sensitivity. A long-term exposure to H2S, reflected by higher foliar S-content, and CO2 might depress the antioxidant capacity of leaves close to the vent and increase their O3 sensitivity. Very high CO2 concentrations did not compensate for the effects of O3 on holm oak photosynthesis.

The beneficial uptake of nutrients by wetland plants is countered to some extent by nutrient release back into the aquatic environment due to vegetative die-back. This current study examined whether Leersia oryzoides, a common wetland plant, exhibits luxury uptake of nutrients from simulated farm runoff. The study also tested whether with subsequent decomposition, these nutrients are released back into the water column. When exposed to elevated (>2 mg/L N and P) runoff, L. oryzoides assimilated significantly higher concentrations of nitrogen (p < 0.001) and phosphorus (p < 0.001) in above-ground biomass as compared to non-enriched treatments (<0.05 mg/L N and P). Subsequently, senescence of enriched above-ground biomass yielded significantly higher concentrations of phosphorus (2.19 ± 0.84 mg P/L). Using L. oryzoides as our model, this study demonstrates nitrogen and phosphorus sequestration during the growing season and release of phosphorus in the winter. Release of sequestered nutrients during plant senescence.

The soil compartment is an important interface between the atmosphere and the subsurface hydrosphere. In this paper a conceptual approach for regional hydrologic soil modelling (RHSM) is presented, which provides two important qualities for modelling. First, the soil compartment is directly coupled to the atmosphere via the land surface and to the aquifers. Second, extremely fine (5 cm vertical) resolutions of the soil system can be realized at regional scales (several hundreds of km2). This high-resolution modelling could be achieved by parallel computation techniques. The RHSM approach is applied to the Beerze-Reusel drainage basin, which belongs to the Meuse River basin. Moisture transport in the soil system was calculated with extremely high vertical resolution at a regional scale based on rainfall-evaporation data for the year 2000. As a result, highly resolved regional groundwater recharge pattern addressing the heterogeneity of soil systems could be determined. A real case application of concept of regional hydrologic soil modelling is presented.

For most trace elements, the technique of phytoextraction needs significant improvements to become practically feasible. Calculations for Cd revealed that the amount of Cd taken up by Thlaspi caerulescens or Salix spp. needs at least to be the double of the present amount to slightly decrease the Cd concentration in the upper 0.5 m of the soil within a period of 10 years. Additionally, metals taken up by the plants might pose an important risk. Alternatives as bioavailable contaminant stripping and phytostabilization might be more appropriate. Phytoextraction efficiency should be improved and associated risks need more attention before phytoextraction can be established as a commercial technology.