Billions of disposable face masks are consumed daily due to the COVID-19 pandemic. The role of these masks as a source of nanoplastics (NPs) and microplastics (MPs) in the environment has not been studied in previous studies. We quantified and characterized face mask released particles and evaluated their potential for accumulation in humans and marine organisms. More than one billion of NPs and MPs were released from each surgical or N95 face mask. These irregularly-shaped particles sized from c. 5 nm to c. 600 μm. But most of them were nano scale sized <1 μm. The middle layers of the masks had released more particles than the outer and inner layers. That MPs were detected in the nasal mucus of mask wearers suggests they can be inhaled while wearing a mask. Mask released particles also adsorbed onto diatom surfaces and were ingested by marine organisms of different trophic levels. This data is useful for assessing the health and environmental risks of face masks.

Prenatal fine particulate matter (PM₂.₅) exposure has been associated with impaired offspring neurodevelopment; however, the association of PM₂.₅ exposure during preconception with offspring’s neurodevelopment and factors responsible for this association are still unclear. This study estimated the associations of PM₂.₅ exposure during preconception and the first trimester with offspring neurodevelopment and evaluated whether maternal thyroid hormones mediate these associations. We recruited 1329 mother-child pairs between 2013 and 2015 in Wuhan, China. PM₂.₅ exposure levels of each woman during the 3 months preconception and the first trimester were estimated using land-use regression models. Offspring neurodevelopment characterized by mental developmental index (MDI) and psychomotor developmental index (PDI) were measured at 24 months of age. Maternal serum levels of free thyroxine (FT3), free triiodothyronine (FT4), and thyroid-stimulating hormone (TSH) during early pregnancy were measured of a subset of the 1329 women (551 women). Generalized estimation equation and general linear regression models were used to estimate the associations between maternal PM₂.₅ exposure, thyroid hormones, and offspring neurodevelopment. After adjusting for potential confounders, we found that either among all participants or the subset, PM₂.₅ exposure during preconception and the first trimester was negatively associated with offspring PDI. Double increment in the first trimester PM₂.₅ exposure was significantly associated with 3.43 and 6.48 points decrease in offspring MDI. In the subset, each doubling of PM₂.₅ exposure during preconception and the first trimester was significantly associated with 7.93 and 8.02 points decrease in maternal FT4 level, respectively. Increased maternal FT4, in turn, was associated with increased PDI (β = 16.69, 95% CI: 5.39, 27.99). About 7.7% (95% CI: 2.0%–19.4%) and 8.6% (95% CI: 3.0%, 22.1%) of the effect of PM₂.₅ exposure during preconception on offspring PDI was mediated through maternal FT4 and the FT4/FT3 ratio, respectively.

Microplastic (MP) pollution poses a huge threat to agroecosystems, but the distribution characteristics of MPs in different types of farmland are still largely unknown. In this work, samples from six land-use types were collected from Chinese farmlands in five provinces. It was found that MP abundances were in the range of 2783–6366 items/kg in all samples. MP distribution results showed that over 80% of particles were less than 1 mm, and that MP sizes ranging between 0.02 and 0.2 mm represented the greatest proportion. The particle shape classified as fragment (with edges and angular) was the most frequent shape, with an abundance of approximately 54.05%. Polyethylene (PE) and polyamides (PA) were the most abundant polymers in cropped lands; 20.88% and 20.31%, respectively. Statistical analyses showed that lands used for plastic mulching (mulch film and greenhouse crops) had a significantly higher particle abundance, hence identifying plastic mulching as a major contributor to MP pollution in agricultural lands. Furthermore, paddy lands had a significantly higher MP abundance than wheat lands. Variation analyses of MP characteristics revealed that cereal crop farmlands (wheat, paddy land) were more likely to contain fibrous shapes and large MP particles (1–5 mm). Economically important tree lands (orchards, woodlands) were likely to contain fragment shapes and pony-size MPs (0.02–0.2 mm). Discrepancies among farmlands may depend on various reasons, such as mulching plastic application, irrigation, atmospheric fallout, etc. This study provides firsthand evidences about the characteristics of MP pollution in farmlands and explores some predominant MP sources in agroecosystems.

The occurrence of human pathogenic viruses in aquatic ecosystems and, in particular, in internal water bodies (i.e., river, lakes, groundwater, drinking water reservoirs, recreational water utilities, and wastewater), raises concerns regarding the related impacts on environment and human health, especially in relation to the possibility of human exposure and waterborne infections. This paper reviews the current state of knowledge regarding severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presence and persistence in human excreta, wastewaters, sewage sludge as well as in natural water bodies, and the possible implications for water services in terms of fecal transmission, public health, and workers’ risk. Furthermore, the impacts related to the adopted containment and emergency management measures on household water consumptions are also discussed, together with the potential use of wastewater-based epidemiology (WBE) assessment as a monitoring and early warning tool, to be applied in case of infectious disease outbreaks. The knowledge and tools summarized in this paper provide a basic information reference, supporting decisions makers in the definition of suitable measures able to pursue an efficient water and wastewater management and a reduction of health risks. Furthermore, research questions are provided, in order to direct technical and public health communities towards a sustainable water service management in the event of a SARS-CoV-2 re-emergence, as well as a future deadly outbreak or pandemic.

Ground-level ozone (O₃) has become the principal air pollutant in Beijing during recent summers. In this context, an investigation of ambient concentrations and variation characteristics of O₃ and its precursors in May and June from 2014 to 2017 in a typical urban area of Beijing was carried out, and the formation sensitivity and different causes of heavy O₃ pollution (HOP, daily maximum 8-h O₃ (MDA8h O₃)>124 ppbv) were analyzed. The results showed that the monthly assessment values of the O₃ concentrations (the 90ᵗʰ percentile MDA8h O₃ within one month) were highest in May or June from 2014 to 2017, and the values presented an overall increasing trend. During this period, the number of O₃ pollution days (MDA8h O₃ > 75 ppbv) also showed an increasing trend. During the HOP episodes, the concentrations of volatile organic compounds (VOCs), nitrogen oxides (NOX), and carbon monoxide (CO) were higher than their respective mean values in May and June, and the meteorological conditions were more conducive to atmospheric photochemical reactions. The HOP episodes were mainly caused by local photochemical formation. From 2014 to 2017, O₃ formation during the HOP episodes shifted from VOC and NOX mixed-limited to VOC-limited conditions, and O₃ formation was most sensitive to anthropogenic VOCs. Six categories of VOC sources were identified, among which vehicular exhaust contributed the most to anthropogenic VOCs. The VOC concentrations and OFPs of anthropogenic sources have decreased significantly in recent years, indicating that VOC control measures have been effective in Beijing. Nevertheless, NOX concentrations did not show an evident decreasing trend in the same period. Therefore, more attention should be devoted to O₃ pollution control in May and June; control measure adjustments are needed according to the changes in O₃ precursors, and coordinated control of VOCs and NOX should be strengthened in long-term planning.

With conventional plastics posing a great threat to marine organisms, and potentially also to humans, bio-based, biodegradable plastics are being offered as an ecological solution by which to reduce the environmental impact. Inside compost facilities, bioplastics that comply with the EN 13432:2000 international standard biodegrade almost completely within 180 days. However, outside compost facilities, and specifically in marine environments, these bioplastics may have a similar effect to that of fossil-fuel based plastics. Here we investigated the effects of polyethylene terephthalate (PET) and polylactic acid (PLA) single-use cups and plates on a solitary ascidian’s biological and ecological features. Both PET and PLA microparticles reduced the fertilization rate of Microcosmus exasperatus, with no significant difference between materials. Accumulation rates in adult M. exasperatus exposed to micronized PET and PLA particles at two concentrations were similar for both the bioplastic material and the conventional plastic particles, with no significant difference between the two materials. A microbial-based digestive protocol was developed in order to recover the bioplastic material from ascidian tissue and reduce any material-loss caused by the known digestion protocols. Finally, PET plates submerged for three months in the Red Sea exhibited a significantly higher community richness and cover area in comparison to PLA plates, which did not provide a firm substrate for settlers. Indeed, coverage by the solitary ascidian Herdmania momus was significantly higher on PET plates. The current study demonstrates that discarded bioplastic products may have similar effects to those of conventional plastics on marine organism fertilization and biological accumulation, emphasizing the need to revise both the production and marketing of “biodegradable” and “compostable” plastics in order to prevent a further negative impact on ecosystems due to the mismanagement of bioplastic products.

As an emerging pollutant, uranium poses serious concerns to ecological and human health. The kidney has been established as a major deposition site and the most sensitive target organ for uranium poisoning, and the underlying toxicological mechanisms have been associated with oxidative stress and mitochondrial respiration. However, the identities of key molecular targets in uranium-induced toxicity remain elusive. In this study, we comprehensively evaluated the in vitro effects of uranium on ten critical enzymes in the mitochondrial respiration pathway and discovered that respiratory chain complex IV (cytochrome c oxidase) and complex V (ATP synthase) were strongly inhibited. The inhibitory effects were validated with mitochondria from human renal proximal tubule cells—the most affected renal site in uranium poisoning. The IC₅₀ values (around 1 mg/L) are physiologically relevant, as they are comparable to known kidney accumulation levels in uranium poisoning. In addition, these inhibitory effects could explain the well-documented uranium-induced reactive oxygen species generation and mitochondrial alterations. In conclusion, cytochrome c oxidase and ATP synthase are possibly key molecular targets underlying the toxic effects of uranium.

Exposures to organic pesticides, particularly during a developmental window, have been associated with various neurodegenerative diseases later in life. Atrazine (ATZ), one of the most used pesticides in the U.S., is suspected to be associated with increased neurodegeneration later in life but few studies assessed the neurotoxicity of developmental ATZ exposure using human neuronal cells. Here, we exposed human SH-SY5Y cells to 0.3, 3, and 30 ppb of ATZ prior to differentiating them into dopaminergic-like neurons in ATZ-free medium to mimic developmental exposure. The differentiated neurons exhibit altered neurite outgrowth and SNCA pathology depending on the ATZ treatment doses. Epigenome changes, such as decreases in 5mC (for 0.3 ppb only), H3K9me3, and H3K27me3 were observed immediately after exposure. These alterations persist in a compensatory manner in differentiated neurons. Specifically, we observed significant reductions in 5mC and H3K9me3, as well as, an increase in H3K27me3 in ATZ-exposed cells after differentiation, suggesting substantial chromatin rearrangements after developmental ATZ exposure. Transcriptional changes of relevant epigenetic enzymes were also quantified but found to only partially explain the observed epigenome alteration. Our results thus collectively suggest that exposure to low-dose of ATZ prior to differentiation can result in long-lasting changes in epigenome and increase risks of SNCA-related Parkinson’s Disease.

This study collected 184 groundwater (GW) samples from 92 wells during the dry and wet seasons, respectively to understand the hydrochemical characteristics, water quality, and risk of GW nitrate (NO₃⁻) to human health in northern Shandong Peninsula (NSP), China. The results showed that GW in the NSP is weakly alkaline and classified as hard fresh water. The mean concentration of NO₃⁻ in GW exceeded 100 mg·L⁻¹, total hardness exceeded 450 mg·L⁻¹, and total dissolved solids (TDS) was less than 1000 mg·L⁻¹. A Piper diagram showed that the water chemistry of GW in the NSP was mainly of the SO₄·Cl-Ca·Mg type. A Gibbs diagram and ion ratio analysis indicated that the weathering of silicate rocks and agricultural production were the dominant factors affecting the hydrochemical characteristics of GW in the NSP, with cation exchange, dissolution of salt rock, and weathering of carbonate rock also making contributions. Na⁺ and Cl⁻ in GW are significantly affected by seawater aerosols in coastal areas. During the wet season, the hydrodynamic conditions of the aquifer are improved, agricultural activities are strengthened, and GW becomes generally homogenized. The water quality index classified the GW quality of the NSP as mainly of medium quality. There was a relatively consistent spatial distribution of water quality during different periods. Water samples of poor water quality were mainly distributed in the lower reaches of the Huangshui River. In addition, total hardness and NO₃⁻ concentrations were the main variables affecting the quality of GW in the NSP. The assessment of the risk NO₃⁻ in GW in the NSP to human health through the ingestion of drinking water demonstrated a significant risk (infants > children > adults). These results indicate the need for local management measures to reduce the potential health risks of GW quality in the NSP.

Phenanthrene (Phe) is a tricyclic polycyclic aromatic hydrocarbon with high bioavailability under natural exposure. However, there are few studies on the reproductive toxicity of Phe in mammals. In this study, male Kunming mice were gavaged once every two days with Phe (5, 50, and 500 ng/kg) for 28 weeks. The accumulation levels of Phe in the testis were dose-dependently increased. Histopathological staining showed that Phe exposure reduced the number of spermatogonia, sperm and Sertoli cells. The percentage of testicular apoptotic cells was significantly increased, which was further verified by the upregulated BAX protein. The expression of the GDNF/PI3K/AKT signaling pathway was downregulated, which might suppress the self-renewal and differentiation of spermatogonial stem cells. Meanwhile, Phe exposure inhibited the expression of Sertoli cell markers (Fshr, WT1, Sox9) and the Leydig cell marker Cyp11a1, indicating damage to the function of Sertoli cells and Leydig cells. Serum estrogen and testicular estrogen receptor alpha were significantly upregulated, while androgen receptor expression was downregulated. These alterations might be responsible for impaired spermatogenesis. This study provides new insights for evaluating the reproductive toxicity and potential mechanisms of Phe in mammals.