Prediction of the sorption behavior of environmental pollutants is of utmost importance within the framework of risk assessments. In this work two approaches are presented with the aim to describe sorption of aromatic substances to geosorbents. First, analytical solutions of kinetic models were fitted to experimental data of batch sorption experiments with aniline and 1-naphthylamine onto animal manure-treated soil and the soil mineral montmorillonite. The models, accounting for equilibrium and nonequilibrium sorption coupled to transformation and/or irreversible sorption processes, could well reproduce the concentration course of the sorbates. Results suggest that the amounts transformed/degraded and irreversibly bound were higher for the soil than for the clay mineral. In the second part, quantum chemical calculations were performed on aniline and 1-naphthylamine interacting with acetic acid, acetamide, imidazole, and phenol as models of functional groups present in humic substances. Molecular modeling showed that formation of hydrogen bonds is the dominating binding mechanism in all modeled complexes, which are energetically very similar between aniline and 1-naphthylamine.

Pot culture experiments were conducted to study the remediation potentials of a newly found accumulator Kalimeris integrifolia Turcz. ex DC. under different cadmium (Cd) concentrations with same fertilizer level, as well as the same Cd dose under different fertilizer doses. At medium (100 g/kg) chicken manure level, Cd concentrations in roots, stems, leaves, inflorescences, and shoots of K. integrifolia grown in the soils contaminated with 2.5, 5, 10, and 25 mg/kg Cd significantly decreased (p < 0.05) in average by 23.8%, 29.9%, 24.0%, 30.1%, and 38.6%, respectively, when compared to those of the pots without addition of chicken manure. In contrast, the medium urea amendment level (1 g/kg) showed no effect on the bioaccumulated Cd concentrations of K. integrifolia regardless of the spiked Cd doses. However, Cd extraction capacities (micrograms per pot) of K. integrifolia shoots were significantly increased (p < 0.05) due to the gain in biomass (more than one- to twofolds) by the soil fertilizing effect of urea and chicken manure. Particularly, Cd extraction capacities (micrograms per pot) of K. integrifolia shoots amended by urea were higher than that of chicken manure. Under the condition of 25 mg/kg Cd addition, shoot biomasses of K. integrifolia were significantly increased (p < 0.05) with the amendment of chicken manure (50, 100, and 200 g/kg) and urea (0.5, 1, and 2 g/kg). As a result, the Cd extraction capacities (micrograms per pot) were increased in treatments even though soil extractable Cd concentrations were significantly decreased (p < 0.05) by amendment with chicken manure and maintained by urea addition. For practical application concerns, chicken manure is better used as phytostabilization amendment owing to its reducing role to extractable heavy metal in soil, and urea is better for phytoextraction.

Instrumented column tests are often used to assess the effectiveness of methods to prevent acid mine drainage (AMD). These tests are seldom duplicated, and this lack of duplicate can cast some doubt about their repeatability and reliability. This paper provides an analysis of column test studies (with duplicates) performed with a commonly used methodology. The paper presents the analysis of two multi-layer covers with capillary barrier effects in which the water retention layer was made of a non-plastic silt and of two cover scenarios involving a single-layer low sulphide cover. The first study compared the cover performance to limit AMD when different cover materials are used, while the second study compared the thickness of covers on cover performance. Statistical comparison between duplicates was made using available geochemical data, hydro-geotechnical data, and gas concentrations. Student’s t-test statistical tools and analysis of variances were used to determine the repeatability of the data. The results indicate that a good reproducibility of the column tests can be achieved with a good set-up methodology and rigorous control of the boundary conditions.

In mussels, stress signals such as heat, osmotic shock and hypoxia lead to the activation of the phosphorylated p38 mitogen activated protein kinase (pp38-MAPK). This stress activated protein has been efficiently used as a biomarker to several natural and anthropogenic stresses. However, what has not been tested is whether differences in gender or size can affect the response of this biomarker. The present study tested whether there was variation in the expression of pp38-MAPK in mussels Perna perna of different gender and size classes when exposed to natural stress conditions, such as air exposure. The results show that gender does not affect the expression of pp38-MAPK. However, size does have an effect, where mussels smaller than 6.5 cm displayed significantly (p < 0.05) lower levels of pp38-MAPK when compared to those larger than 7 cm. Mussels are one of the most used bioindicator species and the use of biomarkers to determine the health status of an ecosystem has been greatly increasing over the years. The present study highlights the importance of using mussels of similar size classes when performing experiments using stress-related biomarkers.

Soils are an important sink for persistent organic pollutants (POPs), and high mountain soils are considered a stable reservoir for many compounds due to their high organic matter content. This study focuses on the small-scale variability on the environmental distribution of dichlorodiphenyltrichloroethane (DDT) in mountain soils. Several soil samples taken from May 2007 to June 2008 in a small area at around 1,900 m a.s.l. (Italian Central Alps) were analyzed for DDT compounds. Pedological analyses were done as well. Organic matter content, soil layer, differences in solar radiation, and sampling period were considered as possible variability factors. Organic matter content can account for a DDT concentration difference of a factor 3 among different sites, soil layer can account for a concentration difference of a factor near 2, differences in solar radiation values do not seem to affect DDT concentrations, whereas the sampling period has the greatest influence with a difference factor of three to four among different sampling dates. Summing all these variability factors together, even though operating on such a small scale, we obtain a predicted spatial variability depending on the considered variables near to one order of magnitude. In particular, it was surprising that seasonal variations could be so great. If this conclusion is to be confirmed in the future, this element must be considered very carefully by scientists and environmental agencies during monitoring campaigns.

Two greenhouse pot experiments were conducted in Agrinion, Greece, using a randomized block design in four replications, respectively, as follows: The first one included five levels of treated municipal wastewater (TMWW), being used as an irrigation water source. The second one, five levels of applied Cl at a constant soil applied Cd level of 10.36 mg/kg soil, the plants being irrigated with fresh well water. The purpose of these experiments was to study the impact of the Cl × Cd interrelationship on planning TMWW reuse, for the irrigation of Brassica oleracea var. Capitata (cabbage) cv F1 Gloria, ehich was used as test plant, in both of these experiments. It was found that the TMWW Cl content, originating mainly from the procedure of wastewater chlorination, was synergistically interrelated with the toxic heavy metal Cd, increasing its soil availability and cabbage plant leaf uptake (edible plant part). As this increase is directly associated with the consumer's health, it was suggested that the TMWW be subjected to dechlorination process or the disinfection be made by ozonation or UV, which do not include Cl.

Sediment cores were analysed from four coastal wetland sites within the Minas Basin, Bay of Fundy to compare mercury speciation and sediment characteristics. The coastal wetland sediments were low in total mercury (mean = 17.4 ± 9.9 ng g−1); however, MeHg concentration was 92 times higher (mean of 249 pg g−1) than intertidal mudflat sediment (mean of 2.7 pg g−1). Total mercury concentrations in intertidal mudflat cores were also low (0.5–23.7 ng g−1) and correlated (Pearson correlation = 0.98; p < 0.01) with % organic carbon; with low concentrations of MeHg present only below depths of 6 cm (mean = 2.7 ± 1.0 pg g−1). Total mercury concentrations were negatively correlated (correlation = 0.56, p < 0.05) with inorganic sulphur (acid volatile sulphides (AVS) and pyrite) while MeHg concentrations were inversely correlated (Pearson correlation = −0.68; p < 0.05) with the pyrite content but not with AVS. Methyl mercury concentrations were not significantly correlated with organic carbon content in the wetland sediments, and mercury-in-biomass enrichment factors were lower (total mercury mean 1.5 ± 1.9 and MeHg mean = 3.6 ± 4.8) than published measurements from mercury polluted sites. Modelling estimates found on average 4.4 times more total mercury mass in the intertidal mudflat sediments relative to vegetated wetlands. A negative relationship was observed between MeHg concentrations (below 20 cm depth) and modelled tidal inundation. The mineral fraction within wetland sediments contained 96.2% of the total mercury mass; however, the highest concentrations of mercury species were in root biomass. This research confirms that vegetated coastal wetlands are key areas for formation of bioavailable methyl mercury, and mercury distribution is tied to organic carbon and sulphur speciation.

Measurements of particle size distributions and PM2.5 from an urban background site in the Central Po Valley are analysed; the site is one of the medium–small-size cities in the central valley, without the direct influence of the metropolitan and industrial area of Milan and of the Adriatic Sea. The data comprise number concentration of particle with diameters ranging between 10 and 700 nm, PM2.5 and main meteorological variables from February to August 2008. Daily cycles of the observed pollutants are analysed, along with auto-correlation function for particle number concentration and principal component analysis (PCA) of all the available variables; finally, the diurnal pattern of PM2.5 low-, medium- and high-pollution events has been investigated. Total particle number concentration showed a daily pattern both in winter and summer, although different between weekdays and Sundays and with wider variations during the cold season. A daily cycle is present for the geometric mean diameter of nucleation mode particles in winter and of nucleation and Aitken mode particles in summer. PM2.5 showed a slight daily pattern for weekdays and Sundays, similar, but lagged, to total particle count cycle. Mixing layer depth resulted the main process controlling PM2.5, although also human activities contribute to PM2.5 concentration and allow some deposition and (re-)mobilisation at the first hours of the day and morning rush hour, respectively, while particle number concentration responds immediately to anthropogenic sources. PCA confirmed the dependence of particle number concentration also on meteorological variables, e.g. mixing layer height, wind speed or atmospheric pressure, showing the important influence of regional meteorology on local pollution conditions. Modena can be considered a representative test area of the effect of the meteorological regime for the Central Po Valley on atmospheric particle concentration patterns, characterised by steady high-background concentration.

Limnologists have regarded temporal coherence (synchrony) as a powerful tool for identifying the relative importance of local-scale regulators and regional climatic drivers on lake ecosystems. Limnological studies on Asian reservoirs have emphasized that climate and hydrology under the influences of monsoon are dominant factors regulating seasonal patterns of lake trophic status; yet, little is known of synchrony or asynchrony of trophic status in the single reservoir ecosystem. Based on monthly monitoring data of chlorophyll a, transparency, nutrients, and nonvolatile suspended solids (NVSS) during 1-year period, the present study evaluated temporal coherence to test whether local-scale regulators disturb the seasonal dynamics of trophic state indices (TSI) in a giant dendritic reservoir, China (Three Gorges Reservoir, TGR). Reservoir-wide coherences for TSICHL, TSISD, and TSITP showed dramatic variations over spatial scale, indicating temporal asynchrony of trophic status. Following the concept of TSI differences, algal productivity in the mainstream of TGR and Xiangxi Bay except the upstream of the bay were always limited by nonalgal turbidity (TSICHL−TSISD <0) rather than nitrogen and phosphorus (TSICHL−TSITN <0 and TSICHL−TSITP <0). The coherence analysis for TSI differences showed that local processes of Xiangxi Bay were the main responsible for local asynchrony of nonalgal turbidity limitation levels. Regression analysis further proved that local temporal asynchrony for TSISD and nonalgal turbidity limitation levels were regulated by local dynamics of NVSS, rather than geographical distance. The implications of the present study are to emphasize that the results of trophic status obtained from a single environment (reservoir mainstream) cannot be extrapolated to other environments (tributary bay) in a way that would allow its use as a sentinel site.

Environmental constrains and anthropogenic changes can contribute simultaneously to the limitation of plant performance in Mediterranean urban areas. Photosynthetic efficiency of Quercus ilex L. leaves from the urban area of Naples was evaluated by comparison with leaves from remote site (Vesuvius National Park). The photosynthetic performance of leaves with different age (current year [CY], 1- [1Y] and 2-year old [2Y]) was assessed by measurements of gas exchange, chlorophyll fluorescence and pigment contents. The photosynthetic activity at the urban site was higher than that at the remote site for the CY leaves, while the opposite was observed for the 1Y and 2Y leaves. The growth of Q. ilex trees was mainly sustained by the CY leaves at the urban site, whereas at the remote site the photosynthetic activity was not affected by leaf ageing. In the urban environment, Q. ilex leaves strongly decreased the photosynthetic performance with leaf ageing as highlighted by leaf gas exchanges, although the photochemistry did not show any significant change. The mature leaves of urban site drove the reductive power deriving from the photochemistry more in non-assimilative processes rather than in carbon assimilative ones, avoiding a strong decrease of PSII photochemical efficiency.