To meet human food and fiber needs in an environmentally and economically sustainable way, we must improve the efficiency of waste, water, and nutrient use by converting vast quantities of agricultural and food waste to renewable bioproducts. This work converts waste cherry pits, an abundant food waste in the Great Lakes region, to biochars and activated biochars via slow pyrolysis. Biochars produced have surface areas between 206 and 274 m²/g and increased bioavailability of Fe, K, Mg, Mn, and P. The biochars can be implemented as soil amendments to reduce nutrient run-off and serve as a valuable carbon sink (biochars contain 74–79% carbon), potentially mitigating harmful algal blooms in the Great Lakes. CO₂-activated biochars have surface areas of up to 629 m²/g and exhibit selective metal adsorption for the removal of metals from simulated contaminated drinking water, an environmental problem plaguing this region. Through sustainable waste-to-byproduct valorization we convert this waste food biomass into biochar for use as a soil amendment and into activated biochars to remove metals from drinking water, thus alleviating economic issues associated with cherry pit waste handling and reducing the environmental impact of the cherry processing industry.

Herbicides improve the productivity of a monoculture by eliminating weeds, although they may also be toxic and have negative effects on non-target organisms, such as amphibians. The present study evaluated the genotoxic, mutagenic, and histopathological hepatic responses of Dendropsophus minutus tadpoles to acute exposure (96 h) to the herbicide glyphosate (GLY, 65, 130, 260 and 520 μg/L) and the surfactant polyoxyethylene amine (POEA, 1.25, 2.5, 5 and 10 μg/L). On average, 174 % more genomic damage was observed in the tadpoles exposed to all concentrations of POEA in comparison with the control, while up to seven times more micronuclei were recorded, on average, at a concentration of 5 μg/L of POEA. All the individuals exposed to 10 μg/L of POEA died. The tadpoles exposed to GLY presented 165 % more DNA damage than the control, on average, at the highest concentrations (260 and 520 μg/L), and up to six times more micronuclei at 520 μg/L. The Erythrocyte Nuclear Abnormality test (ENA) detected a relatively high frequency of cells with lobed nuclei in the tadpoles expose to POEA at 5 μg/L and binucleated cells in those exposed to GLY at 520 μg/L. The hepatic histopathological observations revealed several types of lesions in the tadpoles exposed to both GLY and POEA. Overall, then, the results of the study indicate that both GLY and POEA have potential genotoxic, mutagenic, and hepatotoxic effects in D. minutus tadpoles. We emphasize the need for further studies to monitor the amphibian populations, such as those of D. minutus, which breed in aquatic environments associated with agricultural areas. The release of pollutants into natural habitats may have significant long-term impacts on the survival of anuran tadpoles.

Groundwater in several parts of the world, particularly in developing countries, has been contaminated with Arsenic (As). In search of low-cost As removal methods, the biological oxidation of As(III) and Fe(II) followed by co-precipitation requires detailed investigation for the practical implementation of this technology. The present study investigated the biological oxidation of As(III) and Fe(II) through a combination of laboratory experiments and reactive transport modeling. Batch experiments were conducted to evaluate the As(III) oxidation by Fe-oxidizing bacteria, mainly Leptothrix spp. A fixed-bed down-flow biological column containing inexpensive and readily available coconut husk support media was used to evaluate the combined removal of As(III) and Fe(II) from synthetic groundwater. Oxidation and co-precipitation processes effectively reduced the concentration of As(III) from 500 μg/L to < 10 μg/L with a hydraulic retention time of 120 min. A one-dimensional reactive transport model was developed based on the microbially mediated biochemical reactions of As(III) and Fe(II). The model successfully reproduced the observed As(III) and Fe(II) removal trends in the column experiments. The modeling results showed that the top 20 cm aerobic layer of the column played a primary role in the microbial oxidation of Fe(II) and As(III). The model calibration identified the hydraulic residence time as the most significant process parameter for the removal of Fe and As in the column. The developed model can effectively predict As concentrations in the effluent and provide design guidelines for the biological treatment of As. The model would also be useful for understanding the biogeochemical behavior of Fe and As under aerobic conditions.

The mechanism driving the dissemination of antibiotic resistance genes (ARGs) in drinking water supply systems (DWSSs) with multiple barriers remains poorly understood despite several recent efforts. Phosphorothioate (PT) modifications, governed by dndABCDE genes, occur naturally in various bacteria and involve the incorporation of sulfur into the DNA backbone. PT is regarded as a mild antioxidant in vivo and is known to provide protection against bacterial genomes. We combined quantitative polymerase chain reaction, metagenomic, and network analyses for the water treatment process and laboratory-scale experiments for chlorine treatment using model strains to determine if DNA PT modification occurred in DWSS and facilitated the dissemination of mobilized colistin resistance-1 (mcr-1) and New Delhi metallo-β-lactamase-1 (blaNDM₋₁) in DWSS. Our results indicated that the relative abundance of dndB increased in the effluent, compared with the influent, in the water treatment plants. Presence of dndB copies had a positive correlation with the concentration of chloramine disinfectant. Network analysis revealed Bdellovibrio as a potential host for MCR genes, NDM genes, and dndB in the DWSS. E. coli DH10B (Wild-type with the dndABCDE gene cluster and ΔdndB) model strains were used to investigate resistance to chlorine treatment at the concentration range of 0.5–3 mg/L. The resistance of the wild-type strain increased with increasing concentration of chlorine. DNA PT modification protected MCR- and NDM-carrying bacteria from chloramine disinfection during the water treatment process. The higher relative abundance of ARGs in the effluent of the water treatment plants may be due to the resistance of DNA PT modification to chloramine disinfection, thereby causing the enrichment of genera carrying MCR, NDM, and dndB. This study provides a new understanding on the mechanism of ARG dissemination in DWSS, which will help to improve the performance of drinking water treatment to control the risk associated with antibiotic-resistant bacteria.

Honey bees Apis mellifera forage in a wide radius around their colony, bringing back contaminated food resources that can function as terrestrial bioindicators of environmental pesticide exposure. Evaluating pesticide exposure risk to pollinators is an ongoing problem. Here we apply five metrics for pesticide exposure risk (prevalence, diversity, concentration, significant pesticide prevalence, and hazard quotient (HQ)) to a nation-wide field study of honey bees, Apis mellifera in the United States. We examined samples from 1055 apiaries over seven years for 218 different pesticide residues and metabolites, determining that bees were exposed to 120 different pesticide products with a mean of 2.78 per sample. Pesticides in pollen were highly prevalent and variable across states. While pesticide diversity increased over time, most detections occurred at levels predicted to be of low risk to colonies. Varroacides contributed most to concentration, followed by fungicides, while insecticides contributed most to diversity above a toxicity threshold. High risk samples contained one of 12 different insecticides or varroacides. Exposures predicted to be low-risk were nevertheless associated with colony morbidity, and low-level fungicide exposures were tied to queen loss, Nosema infection, and brood diseases.

The Household Air Pollution Intervention Network trial is a multi-country study on the effects of a liquefied petroleum gas (LPG) stove and fuel distribution intervention on women's and children's health. There is limited data on exposure reductions achieved by switching from solid to clean cooking fuels in rural settings across multiple countries. As formative research in 2017, we recruited pregnant women and characterized the impact of the intervention on personal exposures and kitchen levels of fine particulate matter (PM₂.₅) in Guatemala, India, and Rwanda. Forty pregnant women were enrolled in each site. We measured cooking area concentrations of and personal exposures to PM₂.₅ for 24 or 48 h using gravimetric-based PM₂.₅ samplers at baseline and two follow-ups over two months after delivery of an LPG cookstove and free fuel supply. Mixed models were used to estimate PM₂.₅ reductions. Median kitchen PM₂.₅ concentrations were 296 μg/m³ at baseline (interquartile range, IQR: 158–507), 24 μg/m³ at first follow-up (IQR: 18–37), and 23 μg/m³ at second follow-up (IQR: 14–37). Median personal exposures to PM₂.₅ were 134 μg/m³ at baseline (IQR: 71–224), 35 μg/m³ at first follow-up (IQR: 23–51), and 32 μg/m³ at second follow-up (IQR: 23–47). Overall, the LPG intervention was associated with a 92% (95% confidence interval (CI): 90–94%) reduction in kitchen PM₂.₅ concentrations and a 74% (95% CI: 70–79%) reduction in personal PM₂.₅ exposures. Results were similar for each site. The intervention was associated with substantial reductions in kitchen and personal PM₂.₅ overall and in all sites. Results suggest LPG interventions in these rural settings may lower exposures to the WHO annual interim target-1 of 35 μg/m³. The range of exposure contrasts falls on steep sections of estimated exposure-response curves for birthweight, blood pressure, and acute lower respiratory infections, implying potentially important health benefits when transitioning from solid fuels to LPG.

We investigated the spatio-temporal trends of polycyclic aromatic compounds (PACs) deposition in the Athabasca Oil Sands Region (AOSR) between 2008 and 2017, and applied source apportionment tools to assess sources using snowpacks. Estimated PAC mass deposition was significantly correlated with crude oil production (R² = 0.48, p = 0.03), and increased between 2008 and 2017. Loadings of alkylated PACs c1-, c2-fluorenes/pyrenes and c1-, c3-benzo[a]anthracenes/chrysenes/triphenylenes significantly increased at mid-field sites (25–50 km from central industrial reference site, AR6) (Mann-Kendall, p < 0.05) reflecting physical expansion of the AOSR. The distance from emission sources was important in the deposition of PACs, including the distance from AR6 (R² = 0.69–0.91), nearest petcoke storage (R² = 0.77–0.88), 0.89) and upgrader stack (R² = 0.56–0.61). Source apportionment PAC distribution profiles of the source materials (petcokes, oil sand ores, road dust) did not show unique matching profiles with the snowpacks. However, the minimal presence of retene in petcokes and an abundance of benzo[ghi]fluoranthene in road dust was observed, and suggests potential for these compounds as chemical markers in distinguishing sources. Furthermore, correlations between PACs and selected metal(loid)s in the AOSR snowpacks were assessed to infer potential common sources. Significant positive (p < 0.05) correlations between metal(loid)s enriched in bitumen (vanadium, molybdenum, nickel) and PACs, at near to mid-field (0–50 km from AR6) sites suggests common sources or similar transfer and fate processes. The results of our study convey data necessary for monitoring studies in the constantly developing AOSR, advance our knowledge of PACs profiles in source materials (including the much less studied alkylated PACs and dibenzothiophenes), which will be valuable for other studies related to oil pollution, urban run-off and forest fires.PACs mass deposition increasing between 2008 and 2017 coincident with crude oil production, and retene and benzo[ghi]fluoranthene show potential in distinguishing AOSR sources.

To enable and/or facilitate analysis of microplastics from environmental samples, a purification process is required to reduce the organic matter content. The development of such process has as one main concern, besides achieving efficient organic matter reduction, the preservation of the microplastics. In this study, a three-step method for sewage sludge purification was proposed employing sodium dodecyl sulfate and hydrogen peroxide. The effects of the purification method on seven polymers (LLDPE, HDPE, PP, PS, PET, PA66 and SBR) were evaluated in terms of mass change, surface characteristics, mechanical properties, thermal properties and functional groups change. It was also assessed how the polymers were affected by the purification chemicals without the presence of sewage sludge. The purification process led to changes in all tested plastics, but in different intensities. LLDPE, HDPE, PP, PS and PET did not suffer considerable degradation. PET was more affected by hydrolysis than oxidation. On the other hand, the integrities of PA66 and SBR were noticeably affected. The effects of the purification process were considered to be due to the plasticizer behavior of water and oxidation on PA66 and loss of filler and oxidation on SBR. For both polymers there was a reduction on the tensile strength of around 50–60% after the purification, indicating they could be prone to fragmentate into smaller pieces along the process. After purification, PA66 also started to decompose at a temperature around 10 °C lower comparing to virgin samples. Except for SBR, the presence of sewage sludge and its oxidation was more harmful to the polymers than the purification chemicals without the presence of sewage sludge. This study serves as an evaluation of the effects of the purification process on the degradation of microplastics and a methodology for such assessment when designing a purification process.

Children’s respiratory health are particularly vulnerable to outdoor air pollution, but evidence is lacking on the very acute effects of air pollution on the risk of acute upper respiratory infections (AURI) and acute lower respiratory infections (ALRI) in children. This study aimed to evaluate the risk of cause-specific AURI and ALRI, in children within 24 h of exposure to air pollution. We obtained data on emergency cases, including 11,091 AURI cases (acute pharyngitis, acute tonsillitis, acute obstructive laryngitis and epiglottitis, and unspecified acute upper respiratory infections) and 11,401 ALRI cases (pneumonia, acute bronchitis, acute bronchiolitis, unspecified acute lower respiratory infection) in Brisbane, Australia, 2013–2015. A time-stratified case-crossover analysis was used to examine the hourly association of AURI and ALRI with high concentration (95th percentile) of four air pollutants (particulate matters with aerodynamic diameter <10 μm (PM₁₀) and <2.5 μm (PM₂.₅), ozone (O₃), nitrogen dioxide (NO₂)). We observed increased risk of acute tonsillitis associated with PM₂.₅ within 13–24 h (odds ratio (OR), 1.45; 95% confidence interval [CI], 1.02–2.06) and increased risk of unspecified acute upper respiratory infections related to O₃ within 2–6 h (OR, 1.38, 95%CI, 1.12–1.70), NO₂ within 1 h (OR, 1.19; 95%CI, 1.01–1.40), and PM₂.₅ within 7–12 h (OR, 1.21; 95%CI, 1.02–1.43). Cold season and nigh-time air pollution has greater effects on AURI, whereas greater risk of ALRI was seen in warm season and daytime. Our findings suggest exposures to particulate and gaseous air pollution may transiently increase risk of AURI and ALRI in children within 24 h. Prevention measures aimed at protecting children’s respiratory health should consider the very acute effects of air pollution.

As a novel brominated flame retardant (NBFR), decabromodiphenyl ethane (DBDPE) has been poorly understood for the environmental fate and toxicity in terrestrial invertebrates. For the first time, the bioaccumulation, elimination, metabolism and detoxification of DBDPE in earthworms as well as its potential impacts on soil microbes were investigated. The results showed much higher DBDPE concentrations in casts than in earthworms. The bioaccumulation factor (BAF) and elimination rate constant (kₑ) values were 0.028–0.213 (gdw, worm/gdw, soil) and 0.323–0.452 (day⁻¹), respectively. The detoxifying enzymes (CYP450 and GST) could be induced by DBDPE within the range of exposure dosage, and the activities were significantly increased at 21 d (p < 0.05). The results were identified by GC-ECNI-MS, and it showed that at least eleven unknown peaks were separately observed in the earthworms, which were the biotransformation products of DBDPE in earthworms. Additionally, the damages, including skin shrinkage, setae impairment, and intercellular vacuolization, were clearly observed by SEM/TEM. Based on these data, DBDPE could accumulate in earthworms, yet, with low bioaccumulation ability. Moreover, DBDPE exposure resulted in minimal harmful impacts on microbial activities including microbial biomass C (MBC), Microbial basal respiration (MBR), Urease (US) activity and fluorescein diacetate hydrolase (FDA) activity (p < 0.05). Our findings would provide some essential information for interpreting the ecological risks of DBDPE in soil.