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The influence of nanoplastics on the toxic effects, bioaccumulation, biodegradation and enantioselectivity of ibuprofen in freshwater algae Chlorella pyrenoidosa Texto completo
2020
Wang, Fang | Wang, Bin | Qu, Han | Zhao, Wenxing | Duan, Lei | Zhang, Yizhe | Zhou, Yitong | Yu, Gang
Plastic pollution has become a pressing issue due to its persistence in the environment. Smaller plastics are more easily ingested, potentially exerting greater influences on organisms. In this study, the effects of polystyrene nanoplastics (NP) on the toxic effects, bioaccumulation, biodegradation and enantioselectivity of ibuprofen (IBU) in algae Chlorella pyrenoidosa were explored. The influences on the growth rate, chlorophyll a, total antioxidant capacity (T-AOC), reactive oxygen species (ROS) and lipid peroxidation (MDA) were evaluated after 96 h of exposure to a combination of polystryene NP (1 mg L⁻¹) and IBU (5–100 mg L⁻¹). The results indicated that the inhibitory effect of IBU on C. pyrenoidosa growth was alleviated in the presence of NP. For instance, the 96 h-IC₅₀ value for rac-IBU in the treatment lacking NP was 45.7 mg L⁻¹, and the corresponding value in the treatment containing NP was 63.9 mg L⁻¹. The co-exposure of NP led to a significant enhancement of T-AOC and slight reduction of ROS and MDA compared with the individual exposure (IBU) group, suggesting a decreased oxidative stress. In addition, treatment with NP led to a decreased bioaccumulation and accelerated biodegradation of IBU in C. pyrenoidosa and enhanced removal in the medium. The enantioselective toxicity, bioaccumulation and biodegradation of IBU were observed both in the absence and presence of NP. S-IBU exhibited a greater toxicity, and R-IBU was preferentially accumulated and degraded in C. pyrenoidosa. No interconversion of the two enantiomers occurred regardless of the presence of NP. This consequence implied that the influence of coexistent NP should be considered in the environmental risk assessment of pharmaceuticals and personal care products in aquatic environments.
Mostrar más [+] Menos [-]Prediction of plant species occurrence as affected by nitrogen deposition and climate change on a European scale Texto completo
2020
Wamelink, G.W.W. | Mol-Dijkstra, J.P. | Reinds, G.J. | Voogd, J.C. | Bonten, L.T.C. | Posch, M. | Hennekens, S.M. | de Vries, W.
Plant species occurrence in Europe is affected by changes in nitrogen deposition and climate. Insight into potential future effects of those changes can be derived by a model approach based on field-based empirical evidence on a continental scale. In this paper, we present a newly developed empirical model PROPS, predicting the occurrence probabilities of plant species in response to a combination of climatic factors, nitrogen deposition and soil properties. Parameters included were temperature, precipitation, nitrogen deposition, soil pH and soil C/N ratio. The PROPS model was fitted to plant species occurrence data of about 800,000 European relevés with estimated values for pH and soil C/N ratio and interpolated climate and modelled N deposition data obtained from the Ensemble meteo data set and EMEP model results, respectively. The model was validated on an independent data set. The test of ten species against field data gave an average Pearson’s r-value of 0.79.PROPS was applied to a grassland and a heathland site to evaluate the effect of scenarios for nitrogen deposition and climate change on the Habitat Suitability Index (HSI), being the average of the relative probabilities, compared to the maximum probability, of all target species in a habitat. Results for the period 1930–2050 showed that an initial increase and later decrease in nitrogen deposition led to a pronounced decrease in HSI, and with dropping nitrogen deposition to an increase of the HSI. The effect of climate change appeared to be limited, resulting in a slight increase in HSI.
Mostrar más [+] Menos [-]Physiological, ultrastructural, biochemical, and molecular responses of glandless cotton to hexavalent chromium (Cr6+) exposure Texto completo
2020
Samrana, Samrana | ʻAlī, ʻĀbid | Muhammad, Uzair | Azizullah, Azizullah | Ali, Hamid | Khan, Mumtaz | Naz, Shama | Khan, Muhammad Daud | Zhu, Shuijin | Chen, Jinhong
Glandless cotton can be grown to obtain cotton seeds free of toxic gossypol for use as both food and feed. However, they are not grown normally due to their lesser productivity and higher susceptibility to biotic stress. Great attention has been paid to biotic stresses rather than abiotic stresses on glandless cotton. Chromium (Cr) is a common pollutant of soil and considered a serious threat to plants due to its adverse effects on different functions. Although numerous studies are available on the toxicity of Cr⁶⁺ in various plants. However, its adverse effects and mechanism of toxicity in glandless cotton can seldom be found in the literature. This study examined the Cr⁶⁺ effect on glandless cotton in comparison to glanded cotton. Four pairs of glanded and glandless cotton near-isogenic lines (NILs) were exposed to different doses (0, 10, 50, and 100 μM/L) of Cr⁶⁺ for seven days, and biochemical, physiological, molecular, and ultrastructure changes were observed, which were significantly affected by Cr⁶⁺ at high concentrations in all NILs. The effect of Cr⁶⁺ on ionic contents shows the same trend in glanded and glandless NILs except for manganese (Mn²⁺) that show inhibition in glandless (ZMS-12w and Coker-312w) and enhance in the glanded NIL (ZMS-17). The gene expression of superoxide dismutase (SOD) and peroxidase (POD) revealed similar trends as enzyme activities in glandless NILs. The principal component analysis (PCA) and Agglomerative hierarchical clustering (AHC) results of all NILs from morpho-physiological traits, cluster ZMS-16, and ZMS-17 into Cr⁶⁺ sensitive group. While the glandless NILs have the potential to cope with the Cr toxicity by increasing the antioxidant enzyme activity and their gene expression. This study also revealed that Cr⁶⁺ tolerance in cotton is genotypic and has an independent mechanism in the root that not related to low gossypol.
Mostrar más [+] Menos [-]Geospatial assessment of oil spill pollution in the Niger Delta of Nigeria: An evidence-based evaluation of causes and potential remedies Texto completo
2020
Akinwumiju, Akinola S. | Adelodun, Adedeji A. | Ogundeji, Seyi E.
Based on the archival data on oil facilities, oil spill incidents, and environmental conditions, we researched the plausible causes of oil spill disasters in the Niger Delta of Nigeria between 2006 and 2019. The data were analyzed for geospatial and statistical patterns, using ArcGIS and R programming platforms, respectively. A fuzzy logic algorithm was employed to generate three oil spill disaster models (hazard, vulnerability, and risk). Ordinary Least Square algorithm was adopted to model the relationships between oil spill and two sets of predictor variables: oil facilities (oil well, flow station, and pipeline) and disaster models. We found that, during the 23 years, the Niger Delta experienced 7940 oil spill incidents, of which 67% occurred onshore. A total of 4,950, 501, 855 episodes were attributed to sabotage, corrosion, and equipment failure, with 87%, 62%, and 45% occurring onshore, respectively. Besides, 81% of the 5320 onshore oil spill cases were attributed to sabotage, while corrosion and equipment failure accounted for mere 6% and 7% of the incidents, respectively. The estimated average risk index (R = 0.20) shows that the risk of an oil spill disaster in the Niger Delta is low. Whereas, 5% of the region is characterized by a high risk of oil spill disaster. Furthermore, the regression model infers that the oil spillages exhibit a positive relationship with disaster models and oil facilities at α = 0.10. However, only 16% of the incidents were explained by disaster models, while the oil facilities account for 23% of the total cases, indicating the influence of other factors. To avert further socio-environmental damage in the Niger-Delta, oil theft and sabotage should be curbed, polluted areas are remediated, and an all-inclusive socio-economic development is prioritized.
Mostrar más [+] Menos [-]β-catenin mediates fluoride-induced aberrant osteoblasts activity and osteogenesis Texto completo
2020
Chu, Yanru | Gao, Yanhui | Yang, Yanmei | Liu, Yang | Guo, Zining | Wang, Limei | Huang, Wei | Wu, Liaowei | Sun, Dianjun | Gu, Weikuan
Excess fluoride in drinking water is an environmental issue of increasing worldwide concern, because of its adverse effect on human health. Skeletal fluorosis caused by chronic exposure to excessive fluoride is a metabolic bone disease characterized by accelerated bone turnover accompanied by aberrant activation of osteoblasts. It is not clear whether Wnt/β-catenin signaling, an important signaling pathway regulating the function of osteoblasts, mediates the pathogenesis of skeletal fluorosis. A cross-sectional case-control study was conducted in Tongyu County, Jilin Province, China showed that fluoride stimulated the levels of OCN and OPG, resulting in accelerated bone turnover in patients with skeletal fluorosis. To investigate the influence of fluoride on Wnt/β-catenin signaling pathway, 64 male BALB/c mice were allotted randomly to four groups and treated with deionized water containing 0, 55, 110 and 221 mg/L NaF for 3 months, respectively. The results demonstrated that fluoride significantly increased mouse cancellous bone formation and the protein expression of Wnt3a, phospho-GSK3β (ser 9) and Runx2. Moreover, partial correlation analysis indicated that there was no significant correlation between fluoride exposure and Runx2 protein levels, after adjusting for β-catenin, suggesting that β-catenin might play a crucial role in fluoride-induced aberrant osteogenesis. In vivo, viability of SaoS2 cells was significantly facilitated by 4 mg/L NaF, and fluoride could induce the abnormal activation of Wnt/β-catenin signaling, the expression of its target gene Runx2 and significantly increased Tcf/Lef reporter activity. Importantly, inhibition of β-catenin suppressed fluoride-induced Runx2 protein expression and the osteogenic phenotypes. Taken together, the present study provided in vivo and in vitro evidence reveals a potential mechanism for fluoride-induced aberrant osteoblast activation and indicates that β-catenin is the pivot molecule mediating viability and differentiation of osteoblasts and might be a therapeutic target for skeletal fluorosis.
Mostrar más [+] Menos [-]Using the compound system to synthetically evaluate the enrichment of heavy metal(loid)s in a subtropical basin, China Texto completo
2020
Zhang, Hua | Zeng, Huan | Jiang, Yinghui | Xie, Zhenglei | Xu, Xiaoling | Ding, Mingjun | Wang, Peng
A compound system involving three matrices (water, sediment, and paddy soil) was conceived to determine the potential sources of metal(loid)s (Ti, Fe, Co, Ni, Cu, Zn, As, Cd, Pb, and U) and synthetically evaluate their pollution levels in the Le’an River basin. The result indicated that the established background values (BVs) of paddy soil and sediment in the compound system were obviously higher than those of the upper continental crust (UCC) and soils from Jiangxi Province, a difference which was especially marked for sediment. The concentrations of Cu, Zn, As, Cd in the system had high coefficients of variation (CVs), and metal(loid)s in sediment showed higher levels than those in paddy soil, except for Pb. Cd and Cu in the system had the highest Ef levels, which probably pose a high risk to organisms and the health of local residents. There were significantly linear relationships between the site rank index (SRI) for water and that for sediment or paddy soil, revealing that matrices in the system interacted with each other. Principal component analysis (PCA) and absolute principal component scores and multiple linear regression model (APCS-MLR) results demonstrated that Cu, Zn, As, Cu, Pb, and U enrichments in the system were mainly affected by mining activities and were predominately deposited in sediment. Point pollution sources rather than non-point pollution sources such as mining activities, contributed most of the anthropogenic metal(loid)s to sediment. Both SRI and Hierarchical cluster analysis (HCA) results visually showed that S5, S8, S9, S10, S11, and S12 severe pollution grouped together and scattered through areas with extensive mining activities, while other sites with moderate pollution were spread along the main stream of the Le’an River.
Mostrar más [+] Menos [-]Transcriptional profiles and copper stress responses in zebrafish cox17 mutants Texto completo
2020
Sun, HaoJie | Chen, Mingyue | Wang, Ziyang | Zhao, Guang | Liu, Jing-Xia
While Cox17 functions importantly in copper metalation of cytochrome c oxidase and integral mitochondrial architecture in vertebrates, rare studies have been performed regarding the developmental and physiological characters of vertebrate cox17 mutants. In this study, normal-like developmental phenotype was observed in both cox17Δ6−/− and cox17Δ4−/− homozygous zebrafish mutants, while gene ontology term and pathway analysis of the differentially expressed genes in both mutants showed enrichment in oxidoreductase activity, ion transport, histone methylation, MICOS complex, Wnt signaling, etc. This implied the occurrence of damage to the integral function of Cox17 and change of transcriptomes in the two mutants. Further qRT-PCR and WISH assays revealed the down-regulated expression of Wnt signaling and reduced expression of swim bladder marker genes in the two mutants. Moreover, copper stimulation induced no obvious increase in reactive oxygen species (ROS) or in the expression of hemoglobin marker genes, but further reduced the expression of swim bladder marker genes in the mutants. The integral data in this study suggest that: (1) cox17 mutants cannot activate the response of oxidoreductase to copper stimulation; (2) copper depends on the integral function of Cox17 to induce developmental defects in hemoglobin rather than swim bladder and (3) Wnt signaling but not ROS might mediate copper-induced swim bladder developmental defects in fish.
Mostrar más [+] Menos [-]Trace element analysis reveals bioaccumulation in the squid Gonatus fabricii from polar regions of the Atlantic Ocean Texto completo
2020
Lischka, A. | Lacoue-Labarthe, T. | Bustamante, P. | Piatkowski, U. | Hoving, H.J.T.
The boreoatlantic gonate squid (Gonatus fabricii) represents important prey for top predators—such as marine mammals, seabirds and fish—and is also an efficient predator of crustaceans and fish. Gonatus fabricii is the most abundant cephalopod in the northern Atlantic and Arctic Ocean but the trace element accumulation of this ecologically important species is unknown. In this study, trace element concentrations (Ag, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, and Zn) were analysed from the mantle muscle and the digestive gland tissue of juveniles, adult females, and adult males that were captured south of Disko Island off West-Greenland. To assess the feeding habitat and trophic position of this species, stable isotopes of carbon (δ13C) and nitrogen (δ15N) were measured in their muscle tissue. Mercury concentrations were positively correlated with size (mantle length) and trophic position. The Hg/Se ratio was assessed because Se has been suggested to play a protective role against Hg toxicity and showed a molar surplus of Se relative to Hg. Cadmium concentrations in the digestive gland were negatively correlated with size and trophic position (δ15N), which suggested a dietary shift from Cd-rich crustaceans towards Cd-poor fish during ontogeny. This study provides trace element concentration data for G. fabricii from Greenlandic waters, which represents baseline data for a northern cephalopod species. Within West-Greenland waters, G. fabricii appears to be an important vector for the transfer of Cd in the Arctic pelagic food web.
Mostrar más [+] Menos [-]Development and validation of a UHPLC-MS/MS method for the identification of irinotecan photodegradation products in water samples Texto completo
2020
Gosetti, Fabio | Belay, Masho Hilawie | Marengo, Emilio | Robotti, Elisa
Irinotecan (CPT-11) is a water-soluble anticancer drug widely used to treat several types of cancer.Even if the metabolites of CPT-11 are well-known and investigated, only limited information is available in the literature about the formation of photo-degradation products that can naturally originate from sunlight irradiation when the drug is released in aqueous systems.CTP-11 solutions at 10.0 mg L−1 were irradiated by simulated sunlight. The intensity of the drug decreased by 90% after 7.5 days of irradiation and no significant reduction of absorbance values was observed after 13 days.A sensitive UHPLC-MS/MS method was developed employing a hybrid triple quadrupole/linear ion trap mass spectrometer, that is able to work in data-dependent acquisition mode and to obtain information about the compounds formed during the photoirradiation. Moreover, a selected reaction monitoring method was built using the MS/MS fragmentation pattern of the compounds previously investigated. The method was validated considering LOD, LOQ, linearity, precision, selectivity, recovery and matrix effect. LOD and LOQ values were 0.02 and 0.05 ng mL−1, respectively, whereas MDL and MQL values in real water samples (river water, groundwater, well water, and wastewater) were lower than 0.05 and 0.2 ng mL−1, respectively.Eight photodegradation products were identified, among which five for the first time. Based on the MS and MS/MS fragmentation, the chemical structures of the degradation products were proposed. Hydrolysis experiments were carried out on the same solutions preserved in the dark, but no formation of other species was highlighted.The method was applied to several real samples: CPT-11 was detected and quantified only in a hospital effluent sample at the concentration of 0.41 ± 0.2 ng mL−1 together with the occurrence of PDP3.The outcomes of this study may be useful for updating the pollutant screening in water samples.
Mostrar más [+] Menos [-]A non-invasive method to monitor marine pollution from bacterial DNA present in fish skin mucus Texto completo
2020
Montenegro, Diana | Astudillo-García, Carmen | Hickey, Tony | Lear, Gavin
Marine coastal contamination caused by human activity is a major issue worldwide. The implementation of effective pollution monitoring programs, especially in coastal areas, is important and urgent. The use of biological, physiological, or biochemical measurements to monitor the impacts of pollution has garnered increasing interest, particularly for the development of new non-invasive tools to assess water pollution. Fish skin mucus is in direct contact with the marine environment, making it a favourable microenvironment for the formation of biofilm bacterial communities. In this study, we developed a non-invasive technique, sampling fish skin mucus to determine and analyse bacterial community composition using next-generation sequencing. We hypothesised that bacterial communities associated with the skin mucus of a common harbour benthic blennioid triplefin fish, Forsterygion capito, would reflect conditions of different marine environments. We detected clear differences in bacterial community alpha-diversity between contaminated and reference sites. Beta-diversity analysis also revealed differences in the bacterial community structure of the skin mucus of fish inhabiting different geographical areas. The relative abundance of different bacterial orders varied among sites, as determined by linear discriminant analysis (LDA) and effect size (LEfSe) analyses. The observed variation in bacterial community compositions correlated more strongly with variation in hydrocarbons than to various metal concentrations. Using advanced DNA sequencing technologies, we have developed a novel non-invasive, low-cost and effective tool to monitor the impacts of pollution through analysis of the bacterial communities associated with fish skin mucus.
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