In this study, sea urchin Paracentrotus lividus were sampled seasonally at three stations during 2012 in the coastal areas of the Gulf of Annaba (southeast Mediterranean). For all sea urchins, the gonad index was calculated to determine sea urchin reproductive status. Moreover, a set of biochemical parameters, including biomarkers and oxidative stress parameters, was measured in gonads. The pesticides and physiochemical parameters were measured and dosed in sea water.The results obtained highlighted that the levels of pesticide were generally low and below those commonly applied by environmental quality standards (EQS), indicating that no alarm state is currently present in the Gulf of Annaba. In addition to pollution, seasonal change is an important factor influencing biomarker activity, and the significant increases in biomarker levels in spring are a major observed trend. This activity may also be related to reproductive status. Seasonal variability was confirmed by the significant results of the Kruskal-Wallis test and by the high degree of divergence between seasons in PCA, with a total of 83.83% of variance explained.These results indicate that environmental factors that vary seasonally may affect the antioxidant status of the sea urchin Paracentrotus lividus.

A field survey to collect microplastics with sizes <5mm was conducted in the Southern Ocean in 2016. We performed five net-tows and collected 44 pieces of plastic. Total particle counts of the entire water column, which is free of vertical mixing, were computed using the surface concentration (particle count per unit seawater volume) of microplastics, wind speed, and significant wave height during the observation period. Total particle counts at two stations near Antarctica were estimated to be in the order of 100,000pieceskm−2.

The present investigation aimed to study the effect of monsoonal and anthropogenic influences on the water quality parameters of Puducherry coastal waters. Surface water sampling was performed at three fixed stations in four distinct seasons during 2011. Physical water quality parameters such as salinity and TSM showed strong seasonal and spatial variability. Evaporation and monsoonal runoff seem to be the major controlling forces for these parameters in the coastal waters. Seasonal distribution of the parameters showed a random pattern for nitrate and a well-defined pattern for silicate. Chl-a was minimum during monsoon when high TSM was encountered in the system. Moreover, factors that regulated the phytoplankton biomass varied with seasons. Moreover, TSM was strongly correlated with silicate. The relationship between Chl-a and nutrients were more consistent throughout the year, and much weaker correlations were noticed between Chl-a and TSM. Cluster analysis depicted the existence of a marked seasonal heterogeneity.

Sediments were grouped in three zones of the bay: two in the northern basin with higher metal contamination; and another in the southern basin, where lithogenic metals were predominant. Sediment pollution classification made using Index of geoaccumulation and Enrichment factor was consistent, indicating higher accumulation of Cd and Pb in the northern basin. The negative influence of activities linked to petrol was predicted by V/Ni and V/(Ni+V) ratios. Cd and Pb did not represent a potential risk; while Cu and Ni could be risky for biota in most sediment, according to Screening Quick Reference Tables (SQuiRTs). Comparison of bioavailable fraction of metals with references in SQuiRTs corroborated the low potential damage on the biota due to As, Cd, Pb, Cr, Zn; and a higher potential damage due to Cu; while an attenuation of the risk due to Ni predicted by SQuiRTs could be expected.

This study assessed the impact of derelict fishing gear (DFG) on navigation. The Republic of Korea's navy (ROKN) recorded every case of approximately 170 naval ships associated with propeller entanglement by DFG from January 2010 to December 2015. The frequency of cases was 2.3 per ship and 397.7 (±37.5) per year. The amount of DFG disentangled was 0.025tons per ship and 10.0 (±1.7) tons per year. The frequency temporally decreased whereas the amount increased over these six years. To disentangle propellers, 3.1 divers were needed on average per case. Propeller entanglement occurred in all local seas and some of these areas showed increases over time. Our study highlights that the impact of DFG on navigational threats has been persistent and ubiquitous and can potentially be reduced by preventing DFG in fishing areas, with a focus on improved management by fishermen and government and with more efficient retrieval of DFG.

Over 50 million cubic meters of mining tailings were released in the Rio Doce basin after the collapse of the Fundão dam (Samarco) in November 2015. Predicting significant impacts on the Rio Doce estuary, we sampled sediments to investigate short-term impacts on the benthic assemblages and trace metal accumulation on estuarine sediments. With the arrival of the tailing plumes in the estuary, we detected a predominance of clay particles and increased trace metal concentrations of up to 5 times in some areas. The rapid sedimentation after the impact also impacted estuarine macrofaunal assemblages through loss surface-dwelling taxa. As expected, the impacts on benthic assemblages observed up to 3days after the arrival of tailings were not clearly associated with trace metal concentrations, but long-term effects need to be studied. We recommend that the high spatial variability within the estuary be considered in future impact assessment studies.

The present study extrapolates the assessment and characterization of a barely studied region, the Gulf of Kutch, (near Jamnagar), Gujarat, India, in terms of PAH exposure, adverse effects caused by them, and various toxicological indices showing the catastrophic effects of their elevated concentrations. ΣPAH concentration in the site ranged from 118,280 to 1,099,410ngg−1 dw, with a predominance of 2–3-ring PAHs (79.09%) as compared to 4–5- and 6-ring PAHs (20.91%). The concentrations of carcinogenic PAHs were found to be between 8120 and 160,000ngg−1 dw, with a mean of 63,810ngg−1 dw, which is much higher than normal acceptable values. The toxic equivalent quotient for 6CPAHs ranged from 150.47 to 26,330ngg−1 BaPeq, encompassing 50.63% of ΣPAH toxicity. This toxicological profile of the present study site would be of paramount importance as it offers fresh information regarding the load of legacy pollutants such as PAHs and the inputs and methods to cope with their extremely high concentrations in less explored marine habitats.

In the Marine Protected Area of La Maddalena Archipelago, environmental protection rules and safeguard measures for nautical activities have helped in reducing anthropogenic pressure; however, tourism related activities remain particularly significant in summer. With the aim of evaluating their impacts, the biomarker approach using transplanted Mytilus galloprovincialis as sentinel organisms coupled with POCIS deployment was applied. Mussels, translocated to four marine areas differently impacted by tourism activities, were sampled before, during and after the tourist season. Moreover, endocrine disruptors in passive samplers POCIS and the cellular toxicity of whole POCIS extracts on mussel haemocytes were evaluated to integrate ecotoxicological information. Lysosomal biomarkers, condition index and mortality rate, as well as metals in tissues suggested an alteration of the health status of mussels transplanted to the most impacted sites. The cellular toxicity of POCIS extracts was pointed out, notwithstanding the concentrations of the examined compounds were always below the detection limits.

This study examined the influence of environmental concentrations of Aroclor 1254 (10, 100, and 1000ng/L) on metamorphosis of Paralichthys olivaceus, and analyzed the mechanisms in relation to thyroid disruption. Results showed that 100 and 1000ng/L Aroclor 1254 delayed metamorphosis and that 1000ng/L Aroclor 1254 caused abnormal morphology. Thyroxine and triiodothyronine levels in the control group were significantly elevated at metamorphic climax, but treatment with 100 and 1000ng/L delayed the increase in thyroid hormones (THs) and retarded metamorphic processes. In larvae exposed to 1000ng/L Aroclor 1254, TH levels at metamorphic climax were significantly lower than those of the control group at the same metamorphic stage. We suggest that the effects of Aroclor 1254 on larval metamorphosis can be explained by disruption of thyroid homeostasis. These findings provide a new perspective and biological model for thyroid-disrupting chemicals (TDCs) screening and investigating interference of thyroid function by TDCs.

Measurements of microplastics in biota and abiotic matrices are key elements of exposure and risk assessments for this emerging environmental pollutant. We investigated the abundance of microplastics in field-collected biota, sediment and water. An improved sediment extraction method, based on density separation was developed. For analysis of microplastics in biota we found that an adapted enzymatic digestion protocol using proteinase K performed best, with a 97% recovery of spiked plastic particles and no observed degradation effects on the plastics in subsequent Raman analysis. Field analysis revealed that 8 of 9 tested invertebrate species from the North Sea and 68% of analyzed individuals of brown trout (Salmo trutta) from the Swedish West Coast had microplastics in them. Based on the number of plastic particles per kg d.w. the microplastic concentrations found in mussels were approximately a thousand-fold higher compared to those in sediment and surface water samples from the same location.