This study aimed to analyze the environmental impacts of the oxidative desulfurization (ODS) process catalyzed by metal-free reduced graphene oxide (rGO) through life cycle assessment (LCA). The environmental impacts study containing the rGO production process, the ODS process, the comparison of different oxidants and solvents was developed. This study was performed by using ReCiPe 2016 V1.03 Hierarchist midpoint as well as endpoint approach and SimaPro software. For the production of 1 kg rGO, the results showed that hydrochloric acid (washing), sulfuric acid (mixing), hydrazine (reduction) and electricity were four main contributors in this process, and this process showed a significant impact on human health 14.21 Pt followed by ecosystem 0.845 Pt and resources 0.164 Pt. For the production of 1 kg desulfurized oil (400 ppm), main environmental impacts were terrestrial ecotoxicity (43.256 kg 1,4-DCB), global warming (41.058 kg CO₂), human non-carcinogenic toxicity (19.570 kg 1,4-DCB) and fossil resource scarcity (13.178 kg oil), and the main contributors were electricity, diesel oil and acetonitrile. The whole ODS process also showed a greatest effect on human health. For two common oxidants hydrogen peroxide and oxygen used in ODS, hydrogen peroxide showed a greater impact than oxygen. On the other hand, for three common solvents employed in ODS, N-methyl-2-pyrrolidone had a more serious impact on human health followed by acetonitrile and N,N-dimethylformamide. As such, LCA results demonstrated the detailed environmental impacts originated from the catalytic ODS, hence elucidating systematic guidance for its future development toward practicality.

Urine sample tests are one of the most common methods of estimating human exposure to polycyclic aromatic hydrocarbons (PAHs) and assessing population health risks. To evaluate the reliability of the urine test and the impact of other PAH elimination routes on the health risk estimated by this test, we proposed a backward modeling framework integrating other common elimination routes of PAH metabolites to calculate the overall intake rate of the parent PAH based on the levels of corresponding main metabolites in urine. Due to limited biotransformation data, we selected naphthalene as an example to evaluate model performance and collected urine samples from 234 random adults in Shenzhen. The overall intake rates of naphthalene were then simulated and compared to current literature data. The simulated intake rates of naphthalene ranged from 3.70 × 10⁻³ mg d⁻¹ to 1.95 mg d⁻¹ and followed a lognormal distribution with a median value of 6.51 × 10⁻² mg d⁻¹. The results indicated that, if naphthalene exposure occurred only via food for the population of Shenzhen, the literature data fell within the most frequent interval [3.70 × 10⁻³, 4.45 × 10⁻²] but were lower than the simulated median value. However, if other exposure routes were considered, the allocation factor-adjusted literature data were close to the simulated median values. In addition, under normal physiological conditions, the simulated results were more sensitive to 1-hydroxynaphthalene (1-OHN) and 2-hydroxynaphthalene (2-OHN) levels in urine than other biometric variables, which is due to the limited load of 1-OHN and 2-OHN in human elimination routes. Furthermore, the suggested safety levels of 1-OHN and 2-OHN in urine to protect 99% of the general population of Shenzhen were 6.40 × 10⁻⁶ and 3.75 × 10⁻⁵ mg L⁻¹, which could be used as regulatory indicators based on the high reliability of the model.

In this biomonitoring study, we evaluated the concentrations of 8 polychlorinated biphenyls (PCBs), 11 organochlorinated pesticides (OCPs), 33 brominated flame retardants (BFRs), 7 novel brominated and chlorinated flame retardants (novel FRs) and 30 per- and polyfluoroalkylated substances (PFAS) in human serum samples (n = 274). A total of 89 persistent organic pollutants (POPs) were measured in blood serum samples of city policemen living in three large cities and their adjacent areas (Ostrava, Prague, and Ceske Budejovice) in the Czech Republic. All samples were collected during the year 2019 in two sampling periods (spring and autumn). The identification/quantification of PCBs, OCPs, BFRs, novel FRs and PFAS was performed by means of gas chromatography coupled to (tandem) mass spectrometry (GC–MS/(MS)) and ultra-high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry (UHPLC–MS/MS). The most frequently detected pollutants were perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluorodecanoic acid (PFDA), perfluorooctanesulfonate (PFOS), perfluorohexanesulfonate (PFHxS), 2,2′,3,4,4′,5′-hexachlorobiphenyl (CB 138), 2,2′,4,4′,5,5′-hexachlorobiphenyl (CB 153), 2,2′,3,3′,4,4′,5-heptachlorobiphenyl (CB 170), 2,2′,3,4,4′,5,5′-heptachlorobiphenyl (CB 180), hexachlorobenzene (HCB), and p,p'-dichlorodiphenyldichloroethylene (p,p′-DDE) quantified in 100% of serum samples. In the serum samples, the concentrations of determined POPs were in the range of 0.108–900 ng g⁻¹ lipid weight (lw) for PCBs, 0.106–1016 ng g⁻¹ lw for OCPs, <0.1–618 ng g⁻¹ lw for FRs and <0.01–18.3 ng mL⁻¹ for PFAS, respectively. Locality, sampling season, and age were significantly associated with several POP concentrations. One of the important conclusions was that within the spring sampling period, statistically significant higher concentrations of CB 170 and CB 180 were observed in the samples from Ostrava (industrial area) compared to Prague and Ceske Budejovice. Older policemen had higher concentrations of five PCBs and two OCPs in blood serum.

Particulate matter 2.5 (PM₂.₅) is an inflammatory-inducing factor that is considered to be related to many adverse respiratory problems, especially in the elderly. This study aimed to examine whether pre-exercise training could prevent pulmonary injury induced by urban PM₂.₅ in aging rats and investigate its relationship with inflammatory pathways. Male Wistar rats (aged 16 months) were randomly divided into four groups: sedentary, exercise, sedentary + PM₂.₅ exposure, and exercise + PM₂.₅ exposure. All rats in exercise-related groups were treadmill-trained for 8 weeks (65%–75% VO₂ₘₐₓ for 30 min every other day). Sedentary groups’ rats lived freely in cages without exercise intervention. Rats in the PM-related groups were exposed to ambient PM₂.₅ (4 h day⁻¹) for 2 weeks after an 8-week exercise intervention or sedentary treatment. Finally, all rats’ pulmonary function, lung morphology, degree of inflammation, and relevant protein and mRNA transcript expression levels were examined. The results indicated that PM₂.₅ exposure induced lung injury in the sedentary + PM₂.₅ exposure group, as evidenced by the deterioration of pulmonary function, histopathological characteristics, and inflammatory changes. Aerobic exercise alleviated PM₂.₅-induced airway obstruction, deterioration of pulmonary function, bronchial mucosal exfoliation, and inflammatory responses in aging rats. These effects in exercise groups were associated with the increased expression of intracellular 70 kDa heat shock protein (iHSP70) and the suppression of nuclear transcription factor-κB (NF-κB) activation, as confirmed by increased expression of inhibitor of NF-κB (IκBα) and a reduction in phospho-IKBα (p-IκBα), which is regulated by inhibiting kappa B kinase beta (IKKβ). Taken together, aerobic pre-exercise had protective effects on lung injury and reduced vulnerability to inflammation induced by PM₂.₅ exposure, possibly through the toll-like receptor 4 (TLR4)/NF-κB signaling pathways mediated by the extracellular-to-intracellular HSP70 ratio. Pre-exercise training may be an effective way to protect against PM₂.₅-induced lung toxicity in aging individuals.

Pollution of riverine ecosystems through the multidimensional impact of human footprints around the world poses a serious challenge. Research studies that communicate potential repercussions of landscape structure metrics on snowmelt riverine water quality particularly, in climatically fragile Himalayan watersheds are very scarce. Though, worldwide, grasping the influence of land-use practices on water quality (WQ) has received renewed attention yet, the relevance of spatial scale linked to landscape pattern is still elusive due to its heterogenic nature across diverse geomorphic regions. In this work, therefore, we tried to capture the insights on landscape-aquascape interface by juxtapositioning the impacts of landscape structure pattern on snowmelt stream WQ of the whole Jhelum River Basin (JRB) under three varying spatial scales viz., watershed scale, riparian corridor (1000 m wide) and reach buffer (500 m wide). The percentage of landscape pattern composition and configuration metrics in the JRB were computed in GIS utilizing Landsat-8 OLI/TIRS satellite image having 30 m resolution. To better explicate the influence of land-use metrics on riverine WQ with space and time, we used Redundancy analysis (RDA) and multilinear regression (MLR) modeling. MLR selected land-use structure metrics revealed the varied response of WQ parameters to multi-scale factors except for total faecal coliform bacteria (TC) which showed perpetual presence. The reach-scale explained slightly better (76%) variations in WQ than riparian (75%) and watershed (70%) scales. Likewise, across seasonal scale, autumn (75%), winter (83%), and summer (77%) captured the most WQ variation at catchment, riparian, and reach scales respectively. We observed impairing WQ linkages with agriculture, built-up and barren rocky areas across watersheds, besides, pastures in riparian buffer areas, and fragmentation of landscape patches at the reach scale. Due to little appearance of spatial scale differences, a multi scale perspective landscape planning is emphasized to ensure future sustainability of Kashmir Himalayan water resources.

It is estimated that approximately 0.4% of the total leachate produced in a landfill is destined for treatment plants, while the rest can reach the soil and groundwater. In this context, this study aimed to perform leachate toxicity evaluations through immune system cytotoxic assessments, genotoxic (comet assay) appraisals and antioxidant system (superoxide dismutase - SOD; catalase - CAT, glutathione-S-transferase - GST; reduced glutathione - GSH and metallothionein - MT) evaluations in Eisenia andrei earthworms exposed to a Brazilian leachate for 77 days. The leachate sample contained high organic matter (COD - 10,630 mg L⁻¹) and ammoniacal nitrogen (2398 mg L⁻¹), as well as several metals, including Ca, Cr, Fe, Mg, Ni and Zn. Leachate exposure resulted in SOD activity alterations and increased CAT activity and MT levels. Decreased GST activity and GSH levels were also observed. Antioxidant system alterations due to leachate exposure led to increased malondialdehyde levels as a result of lipid peroxidation after the 77 day-exposure. An inflammatory process was also observed in exposed earthworms, evidenced by increased amoebocyte density, and DNA damage was also noted. This study demonstrates for the first time that sublethal effect assessments in leachate-exposed earthworms comprise an important tool for solid waste management.

Microplastics (MPs) are abundant in marine environments, drawing global attention from scientists and rendering it significant to review the research progress and predict future trends of this field. To achieve that, we collected 1898 publications on marine MPs from Web of Science and performed a bibliometric analysis by CiteSpace and VOSviewer. Additionally, we utilized an unrestricted retrieval of literature from ScienceDirect to supplement our major findings. Trends in publication numbers show the growth in study from the initial stage (2012 and before), when microplastic (MP) occurrence, abundance, and distribution were primarily investigated. Throughout the ascent stage (between 2013–2016), when diverse sampling and analytical methods were applied to capture and identify MPs from the ocean, baseline data have been gleaned on physiochemical properties of MPs. The research focus then shifted to the bioaccumulation and ecotoxicological effects of MPs on marine biota, further highlighting their potential deleterious impacts on human health via dietary exposure, and this period was defined as the exploration stage (2017 and onwards). Nevertheless, key challenges including the lack of standard procedures for MP sampling, technical limitations in MP detecting and identification, and controversy about the underlying effects on the marine ecosystems and humans have also been arisen in the last decade. The present study elucidates how we gradually recognize MP pollution in marine environments and what challenges we face, suggesting future avenues for MP research.

The occurrence of veterinary antibiotics and hydro-chemical parameters in eleven natural springs in a livestock production area is evaluated, jointly with the characterization of their DOM fingerprint by Orbitrap HRMS. Tetracycline and sulfonamide antibiotics were ubiquitous in all sites, and they were detected at low ng L⁻¹ concentrations, except for doxycycline, that was present at μg L⁻¹ in one location. DOM analysis revealed that most molecular formulas were CHO compounds (49 %–68 %), with a remarkable percentage containing nitrogen and sulphur (16 %–23 % and 11 %–24 %, respectively). Major DOM components were phenolic and highly unsaturated compounds (~90 %), typical for soil-derived organic matter, while approximately 11 % were unsaturated aliphatic, suggesting that springs may be susceptible to anthropogenic contamination sources. Comparing the DOM fingerprint among sites, the spring showing the most different profile was the one with surface water interaction and characterized by having lower CHO and higher CHOS formulas and aliphatic compounds. Correlations between antibiotics and DOM showed that tetracyclines positively correlate with unsaturated oxygen-rich substances, while sulfonamides relate with aliphatic and unsaturated oxygen-poor compounds. This indicates that the fate of different antibiotics will be controlled by the type of DOM present in groundwater.

Industrial boilers are a significant anthropogenic source of air pollutant emissions. In this study, a county-based atmospheric emission inventory of particulate matter (PM), PM₁₀, PM₂.₅, SO₂, NOx, organic carbon (OC) and elemental carbon (EC) from industrial boilers over mainland China in 2017 was developed for the first time, based on county-level activity data from ~61,000 coal-fired industrial boilers (CFIBs), ~44,000 biomass-fired industrial boilers (BFIBs), ~71,000 gas-fired industrial boilers (GFIBs) and ~9300 oil-fired industrial boilers (OFIBs), updated emission factors (EFs) and air pollution control device (APCD) efficiencies. The total national PM, PM₂.₅, PM₁₀, SO₂, NOx, OC and EC emissions from industrial boilers in 2017 were estimated to be 1,240, 347, 761, 1,648, 1,340, 13.1 and 15.8 kilotons (kt), respectively. Intensive air pollutant emissions from industrial boilers of more than 1000 kg/km² were predominantly in north-eastern, northern and eastern China. CFIBs contributed the most (77.6–94.0 %) to air pollutant emissions because of their high air pollutant EFs and the large amounts of coal consumed. BFIBs were the second-highest contributor to national air pollutant emissions, with the contribution of BFIBs to PM₂.₅, OC and EC emissions in central and southern China reaching up to 42.1 %, 61.7 % and 45.5 %, respectively. There were seasonal peaks in monthly air pollutant emissions in heating regions. The overall uncertainty realting to the new emission inventory was estimated as −25.9 %–22.7 %. Significant air pollutant emission reductions were obtained from 2017 to 2030, and by 2030 the PM, PM₁₀, PM₂.₅, SO₂ and NOx emissions were forecast to decrease by 40.1–84.0 %, 41.6–84.3 %, 44.5–75.2 %, 44.5–75.2 % and 19.5–46.8 % compared to 2017, respectively, under four proposed scenarios. The results of this study showed that differentiated industrial boiler management measures should be developed according to the actual emission characteristics.This work developed a county-based atmospheric emission inventory of PM, PM₁₀, PM₂.₅, SO₂, NOx, OC and EC from Chinese industrial boilers in 2017 for the first time.

Hexafluoropropylene oxide dimer acid (HFPO-DA) is the substitute for perfluoro octanoic acid (PFOA), and recently it has been detected in environmental water samples worldwide and has multiple toxicities. However, whether it will affect the intestines and gut microbiota remains unclear. In this study, in order to evaluate the gut toxicity of HFPO-DA in mammals, male mice were orally exposed to 0, 2, 20, 200 μg/L HFPO-DA, respectively, for 6 weeks. Our results showed that HFPO-DA exposure caused colonic inflammation which was coupled with increased TNF-α levels in serum and increased mRNA expression levels of TNF-α, p65, TLR4, MCP-1 of the colon in mice after exposure to 200 μg/L HFPO-DA. We also found that HFPO-DA exposure induced the decreased mRNA expression levels and protein levels of MUC2 and ZO-1, which means the dysfunction of gut barrier in the colon. In the ileum, we found that HFPO-DA exposure induced the increased mRNA expression levels of various inflammatory factors, but no obvious changes was found to barrier function. Additionally, HFPO-DA exposure caused the imbalance of cecal gut microbiota and changes of cecal microbiota diversity. Taken together, all these results indicate the potential gut toxicity of HFPO-DA and is perceived as a major problem of health risk that affects the inflammation, gut barrier dysfunction, and gut microbiota disturbance in mammals.