Neurodevelopmental exposure to chlorpyrifos (CPF) could increase risks for neurological disorders, such as autism spectrum disorder, cognitive impairment, or attention deficit hyperactivity disorder. The potential involvement of microglia reactive to inflammatory stimuli in these neurological disorders has been generally reported. However, the concrete effects and potential mechanisms of microglia dysfunction triggered by developmental CPF exposure remain unclear. Therefore, we established mouse and human embryonic microglial cells (HMC3 cell) models of developmental CPF exposure to evaluate the effects of developmental CPF exposure on neuroinflammation and underlying mechanisms. The results showed that developmental exposure to CPF enhanced the expression of Iba1 in hippocampus. CPF treatment increased inflammatory cytokines levels and TSPO expression in hippocampus and HMC3 cells. The levels of necroptosis and necroptosis-related signaling RIPK/MLKL were increased in hippocampus and HMC3 cells following CPF exposure. Furthermore, the expression of TLR4/TRIF signaling was increased in hippocampus and HMC3 cells subjected to CPF exposure. Notably, the increased levels of TLR4/TRIF signaling, RIPK/MLKL signaling, necroptosis and pro-inflammatory cytokines induced by CPF treatment were remarkably inhibited by TAK-242 (a specific TLR4 inhibitor). Additionally, the necroptosis and pro-inflammatory cytokines production induced by CPF treatment were significantly relieved by Nec-1 (a specific RIPK1 inhibitor). In general, the above results suggested that activated microglia in hippocampus subjected to developmental CPF exposure underwent RIPK1/MLKL-mediated necroptosis regulated by TLR4/TRIF signaling.

Plastic pollution is one of the most impactful human interferences in our planet. Fragmentation of plastic leads to nano- and microplastics (NP/MP) formation, which accumulate in agricultural lands, representing an increasing risk for crop production and food safety. It has been shown that MP promote damage in plant tissues by several direct and indirect ways, and that NP can enter the tissues/cells and accumulate in edible organs. Investigation of the phytotoxic effects of NP/MP in plants started only in 2016, with most of the studies performed with crops. Since contradictory results are often observed, it is important to review the literature in order to identify robust effects and their possible mechanisms. In this review, we discuss the potential of NP/MP in damaging crop species, with focus on the physiological changes described in the literature. We also performed scientometrics analyses on research papers in this field during 2016–2021, to reveal the research situation of phytotoxic effects of plastic pollution in crops. Our review is as a starting point to help identify gaps and future directions in this important, emerging field.

Generally, Zn in stormwater runoff is considered as low toxicity, but in the senarios of roads and zinc-based materials roof runoff, the concentration of Zn becomes extremely high and cannot be ignored. Bioretention systems are used to remove heavy metals from stormwater runoff, while Zn adsorption is insufficient by conventional filler and is prone to secondary release when exposed to acid rain or high salinity runoff. This study integrated batch experiments and density functional theory calculation to investigate the mechanisms of how KOH-modified biochar (KBC) influences the removal and release of Zn in bioretention systems. The results revealed that KBC adsorbed 89.0–97.5% Zn in the influent, the main adsorption mechanism were complexation and precipitation, and precipitation is more important. In addition, 67% of Zn was immoblized as the residual form by KBC. In acidic and saline runoff, KBC reduced Zn secondary release by 43.6% and 37.08% compared to the results in the absence of KBC, which was attributed to the convertion of most dissolved Zn in acidic and saline runoff into residual Zn. Therefore, KBC has a considerable application potential not only to decontaminate the runoff of roads and Zn-containing roofs, but also to deal with secondary Zn release in acid rain or under the treatment of snow-melting agents.

Reconstructing the long-term Hg history in major emission countries is important for understanding the global Hg cycle and controlling Hg pollution. In this study, the atmospheric Hg history was reconstructed over the last three centuries based on three lacustrine sediment records from southeastern Inner Mongolia in North China, and its relationship with global and regional Hg emissions was revealed. These records show little Hg pollution in the 18th and 19th centuries. This implies a limited influence of Hg emitted from Europe and North America in this region, which is confirmed by their different Hg trends during the two World Wars and the post-1970s. Atmospheric Hg in the region had increased gradually since the 1900s, primarily contributed by emissions from the former Soviet Union in Lake Dalihu (DLH) and Lake Zhagesitai (ZGST) and from the Beijing-Tianjin-Hebei region in Lake Kulunnao (KLN). In the last century, two decreases in Hg fluxes occurred in the KLN core due to the economic recession in the 1960s–1970s and reduced energy consumption and industrial production in the 1990s. In the DLH and ZGST cores, only one decrease occurred, corresponded with the dissolution of the Soviet Union in the 1990s. Although atmospheric Hg emissions in China had stabilized or even decreased in the last decade, atmospheric Hg continued to increase, particularly in KLN, because of emissions from small cities in the region. This study can help understand Hg sources and control Hg pollution in North China and supplement the understanding of the global Hg cycling.

Co-pyrolysis of sewage sludge (SL) with plant biomass gains attention as a way to minimize SL-derived biochar drawbacks, such as high amount of toxic substances, low specific surface area and carbon content. The toxicity of soil amended with SL- (BCSL) or SL/biomass (BCSLW)-derived biochar was evaluated in long-term pot experiment (180 days). The results were compared to SL-amended soil. Biochars produced at 500, 600, or 700 °C were added to the soil (podzolic loamy sand) at a 2% (w/w) dose. Samples were collected at four different time points (at the beginning, after 30, 90 and 180 days) to assess the potential toxicity of SL-, BCSL- or BCSLW-amended soil. The bacteria Aliivibrio fischeri (luminescence inhibition – Microtox), the plant Lepidium sativum (root growth and germination inhibition test – Phytotoxkit F), and the invertebrate Folsomia candida (mortality and reproduction inhibition test – Collembolan test) were used as the test organisms. Depending on the organism tested and the sample collection time point variable results were observed. In general, SL-amended soil was more toxic than soil with biochars. The leachates from BCSLW-amended soil were more toxic to A. fischeri than leachate from BCSL-amended soil. A different tendency was observed in the case of phytotoxicity. Leachate from BCSL-amended soil was more toxic to L. sativum compared to BCSLW-amended soil. The effect of biochars on F. candida was very diversified, which did not allow a clear trend to be observed. The toxic effect of SL-, BCSL- or BCSW-amended soil to particular organisms was observed in different time, point's periods, which may suggest the different factors affecting this toxicity.

Effective immobilization of industrial waste into biochar development could be one of the most promising technologies for solid waste management to achieve circular economy. In this study, post-industrial cotton textile waste (PICTW), a cellulose rich industrial waste, was subjected to slow pyrolysis to develop a surface engineered biochar through phosphoric acid impregnation. Biochar produced at 500 °C designated as PICTWB500 showed a maximum methylene blue number (240 mg g⁻¹) with remarkable specific surface area of 1498 m² g⁻¹. FESEM, FTIR, XRD and Raman spectra analysis were performed to investigate the surface texture and functionalities developed in the biochar. Adsorption efficiency of the biochar was assessed using drimarene red, blue, violet, and black dyes as model dye pollutants in batch mode at different biochar dose, pH and contact time. The maximum monolayer adsorption capacity was obtained in the range 285–325 mg g⁻¹ for different dyes, determined from Langmuir adsorption model. The kinetic behaviour was more favourable with the pseudo second-order model. The recycling ability of PICTWB500 was proven to be effective up to 6th cycle without compromising its adsorption efficiency significantly. This study demonstrated an excellent adsorption capability of the biochar in dye laden real textile effluent and recycling of spent biochar as a precursor of bio compost. Hence, this study established a dual win strategy for waste utilization in textile industry using a closed loop approach with substantial techno-economic feasibility that may have potential applications.

The analysis of microplastics in complex environmental samples requires the use of chemicals to reduce the organic matrix. This procedure should be evaluated in terms of the preservation of the microplastic's integrity, typically done with pristine reference microplastics. However, real microplastics are most likely degraded due to weathering, so pristine reference microplastics might not depict the appropriateness of the process. This study performed a purification process using sodium dodecyl sulfate and hydrogen peroxide on sewage sludge containing LLDPE, HDPE, PP, PS, PET, PA66 and SBR samples exposed to simulated environmental weathering. The degradation of the polymers was assessed by analyzing surface morphology, mass variation, and mechanical, thermal and chemical properties. Comparison with pristine polymers revealed that the purification process can lead to more detrimental effects if the polymers are weathered. After the purification process, some important observations were: 1) LLDPE, PP and SBR surfaces had cracks in the weathered samples that were not observed in the pristine samples, 2) weathered LLDPE, PP and PA66 experienced greater mass loss than pristine, 3) the fragmentation propensity of weathered LLDPE, HDPE, PP, PS and SBR increased compared to pristine samples and 4) the main characteristic peaks in FTIR spectrum could be identified and used for chemical identification of most polymers for pristine and weathered samples. Based on the findings of this study, when analyzing the efficiency and adequacy of a purification process with methods based on surface morphology, mass variation and particle counting indicators, it is recommended to consider the differences that potentially arise between pristine and weathered microplastics, especially for polyolefins (PEs and PP).

Antibiotics are emerging contaminants in aquatic environments which pose serious risks to the ecological environment and human health. Advanced oxidation processes (AOPs) based on ultraviolet (UV) light have good application prospects for antibiotic degradation. As new and developing UV-AOPs, UV/chlorine and derived UV/chloramine processes have attracted increasing attention due to the production of highly reactive radicals (e.g., hydroxyl radical, reactive chlorine species, and reactive nitrogen species) and also because they can provide long-lasting disinfection. In this review, the main reaction pathways of radicals formed during the UV/chlor (am)ine process are proposed. The degradation efficiency, influencing factors, generation of disinfection by-products (DBPs), and changes in toxicity that occur during antibiotic degradation by UV/chlor (am)ine are reviewed. Based on the statistics and analysis of published results, the effects caused by energy consumption, defined as electrical energy per order (EE/O), increase in the following order: UV/chlorine < UV/peroxydisulfate (PDS)< UV/H₂O₂ < UV/persulfate (PS) < 265 nm and 285 nm UV-LED/chlorine (EE/O). Some inherent problems that affect the UV/chlor (am)ine processes and prospects for future research are proposed. The use of UV/chlor (am)ine AOPs is a rich field of research and has promising future applications, and this review provides a theoretical basis for that.

The geochemistry of iron (Fe), manganese (Mn) and sulfur (S) and their effects on arsenic (As) mobility in the mudflats of small river estuaries remain unclear. Here, diffusive gradient in thin films (DGT) and high-resolution dialysis (HR-Peeper) techniques combined with a sequential extraction procedure (BCR) were employed to investigate As, Fe, Mn and S geochemistry in the mudflat of the Jiuxi River estuary, Southeast China. Grain size analysis indicated that fine-grained particles were likely to be deposited in the estuarine intertidal zone and coastal area. DGT and HR-Peeper results revealed that in the estuary and coastal area, the dissolved As in sediment in summer was controlled by Mn geochemistry, which includes not only the release of As through Mn/Fe reduction but also the stabilization of dissolved As in pore water. This stabilization of dissolved As may due to the formation of As–Mn-OM complexes. In winter, the significant positive correlations between DGT-Fe, DGT-Mn, DGT-As and DGT-S indicated that sulfate reduction was the start of As mobilization in sediment in winter. In both the estuary and the coastal area, the easily reducible Fe, Mn and As contents in intertidal sediment were higher than those in the subtidal zone. Combined with the As flux across the sediment-overlying water interface (SWI), these phenomena suggested that As in subtidal sediment diffused into overlying water and that As in overlying water tended to accumulate in the intertidal sediment. The total organic carbon content (TOC) and DGT results in the lower reach, estuary and coastal areas indicated that organic matter is the controlling factor of Fe/Mn reduction, sulfate reduction and As mobilization. The BCR test results showed higher reactive fraction contents of Fe, Mn and As in winter sediment, which threaten the overlying water quality.

Paraquat, a widely used herbicide, causes environmental pollution, and liver injury in humans and animals. As a natural compound in fruits, ellagic acid (EA) shows anti-inflammatory and antioxidant effects. This study examines the beneficial effects of dietary EA against the paraquat-induced hepatic injury and further explores the underlying molecular mechanisms using a piglet model. Post-weaning piglets are fed basal diet supplemented with 50 mg/kg, 100 mg/kg, or 200 mg/kg EA for 3 weeks. At week 2, hepatic injury is induced by 4 mg/kg paraquat followed by 7 days recovery. EA supplementation significantly mitigates paraquat-induced hepatic fibrosis, steatosis, and high apoptotic rate. In agreement, EA supplementation reduces serum pro-inflammatory levels, ameliorates inflammatory cells infiltration into hepatic tissue, which are associated with suppressed NF-κB signaling during paraquat exposure. In addition, EA supplementation significantly improves activities of antioxidative enzymes which were correlated with activated Nrf2/Keap 1 signaling during paraquat exposure. Furthermore, EA supplementation restores cecal microbial community during paraquat exposure. The protective effect of EA is strongly linked with increased relative abundance of Lactobacillus reuteri and Lactobacillus amylovorus. Taken together, EA supplementation effectively reduced the occurrence of hepatic oxidative damage and inflammation induced by paraquat through modulating cecal microbial communities, which provides a novel nutritional therapeutic strategy for hepatic injury.