Existing studies have typically investigated only the association between single pollutants and health outcomes. However, in the real world, people are exposed to multiple air pollutants simultaneously. The effect of air pollutants on emergency department (ED) visits has not been previously studied in the Sichuan Basin, which is one of the most polluted areas. We collected nonaccidental, respiratory and cardiovascular daily ED visits and daily concentrations of PM₂.₅, PMc, CO, SO₂, NO₂ and O₃ in Chengdu, China, from 2014 to 2016. A weighted variable for the combination of multiple air pollutants was constructed to assess the joint adverse health effects. Each air pollutant was assigned a health-related weight, which indicated the pollutant’s relative contribution to the joint effect. The effects on specific subpopulations (males and females; 15–65 years old and >65 years old) were also examined. With an increase of 10 μg/m³ of the combined multiple air pollutants, the daily ED visits for nonaccidental, respiratory and cardiovascular causes increased by 0.96% (95% CI: 0.51%–1.39%), 1.19% (95% CI: 0.53%, 1.85%) and 4.36% (95% CI: 1.06%, 7.76%) at lag 1, respectively. Males presented more pronounced effects, except for cardiovascular disease, than females. Elderly individuals were found to be more sensitive than young individuals. For nonaccidental and respiratory diseases, the contributions of particulate matter (PM) were dominant among the air pollutants, whereas cardiovascular disease was mainly affected by gaseous air pollutants. The combination of multiple air pollutants was significantly associated with ED visits in the Sichuan Basin, China. The joint effect of the combination of multiple air pollutants was highest for cardiovascular disease at lag 1. The relative contributions of individual pollutants varied by disease and subpopulation. These findings suggest that under different pollution scenarios, preventive strategies should target those with different diseases and different subpopulations.

Copper oxide nanoparticles (CuO NP) have been produced on a large scale due to their economically interesting thermophysical properties. This heightens the concern about risks they may pose on their release into the environment, possibly affecting non-target organisms. Microalga are important organisms in ecotoxicological studies as they are at the base of the aquatic food chain, but information about their biochemical and photosynthetic changes in response CuO NP are still scarce. We studied the effects of CuO NP in Raphidocelis subcapitata using morphological, photosynthetic and biochemical biomarkers. Our results showed that the NP affected microalgal population growth with 0.70 mg Cu L⁻¹ IC₅₀–₉₆ ₕ (inhibition concentration). Based on predicted environmental concentrations of Cu NPs in aquatic environments, our results indicate potential risks of the NP to microalgae. Algal cell size, granularity and photosynthetic efficiencies were affected by the CuO NP at 0.97 and 11.74 mg Cu L⁻¹. Furthermore, lipid metabolism was affected mostly at the highest NP concentration, but at environmentally relevant values (0.012 and 0.065 mg Cu L⁻¹) the production of sterols (structural lipids) and triacylglycerols (reserve lipid) increased. Moreover, we found evidence of cell membrane impairment at the highest CuO NP concentration, and, as a photosynthetic response, the oxygen evolving complex was its main site of action. To the best of our knowledge, this is the first study to date to investigate microalgal lipid composition during CuO NP exposure, showing that it is a sensitive diagnostic tool. This research demonstrated that CuO NP may affect the physiology of R. subcapitata, and because they were observed in a primary producer, we foresee consequences to higher trophic levels in aquatic communities.

Oxidative stress is considered a main commonly reported mechanism of nanoparticles toxicity, so this study aimed to evaluate oxidative stress and biochemical alterations in the haemolymph and digestive gland of snail, Monacha cartusiana exposed to sublethal concentrations of zinc oxide nanoparticles (ZnONPs) for 14 days (d). The results indicated that, ZnONPs induced significant increases in lipid peroxidation (LPO) and lactate dehydrogenase (LDH) in treated animals and did not return to normal levels after recover period. A significant decline of glutathione peroxidase (GPx), glutathione-S-transferase (GST) activities, and glutathione (GSH) content in the haemolymph and digestive gland of snails was observed when compared with control. A significant increase was observed in catalase (CAT), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activities of treated animals. In general, nano-materials are able to induce oxidative stress in exposed animals. The present findings indicate that, alterations of antioxidant enzyme activities, increase of LPO, LDH, and reducing of GSH content and GST, GPx activities are recognized to oxidative stress and cell damage. This species could be considered a good bioindicator to assess nano-materials exposure.

Data (N = 10644) for US adults aged ≥20 years for 2003–2016 from National Health and Nutrition Examination Survey were analyzed to evaluate the impact of co-occurrence of obesity with diabetes, anemia, albuminuria, and hypertension on concentrations of five perfluoroalkyl acids (PFAA), namely, perfluorooctanoic acid (PFOA), perfluorooctane sulfonic acid (PFOS), perfluorodecanoic acid (PFDA), perfluorohexane sulfonic acid (PFHxS), and perfluorononanoic acid (PFNA). For the total population, males, and females, co-occurrence of obesity with hypertension, albuminuria, anemia, and diabetes was found to be associated with lower adjusted geometric means (AGM) than nonobese for every PFAA. For example for females, for PFOS, AGMs for obese with no diseases, hypertension, albuminuria, anemia, and diabetes were 8.2, 10.8, 5.8, 4.6, and 7.7 ng/mL respectively. In comparison, for PFOS, for nonobese females, AGMs for those with no diseases, hypertension, albuminuria, anemia, and diabetes were found to be 8.9, 13.4, 7.7, 6.0, and 10.2 ng/mL respectively. This implies obesity is associated with higher excretion rates. Females, in general, had lower AGMs than males for both obese and nonobese for every PFAA for every disease group. For example, percent ratios of obese females to males AGMs for PFOA were 66.7%, 87.1%, 88.2%, 70.6%, and 90% for those with no diseases, hypertension, albuminuria, anemia, and diabetes respectively. The ratios of obese to nonobese AGMs for females were lower than males for every PFAA for those with no diseases and hypertension only. For example, for PFOA for those with no diseases, obese to nonobese AGM ratios were 87% for females and 100% for males. Thus, additional excretion of certain PFAAs due to obesity is higher in females than males for those with no diseases and hypertension only.

The spatial distributions of mercury (Hg) and zinc (Zn) concentration and the isotopic composition of plutonium (Pu) were investigated in surface sediments and sediment cores collected from the Southern Yellow Sea (SYS) during May 2014. The variation of the ²⁴⁰Pu/²³⁹Pu atom ratio (0.18–0.31) in the surface sediments of the SYS clearly indicated a signal of close-in fallout input from the Pacific Proving Ground (PPG). The buried ²³⁹⁺²⁴⁰Pu in the sediment of the SYS was estimated to be (4.7 ± 0.5) × 10¹⁰ Bq y⁻¹ during the period from 2011 to 2014, of which ∼33% (1.5 × 10¹⁰ Bq y⁻¹) was derived from the PPG by long-range transport via ocean currents (e.g., the North Equatorial Current and Kuroshio Current). The concentrations of Hg and Zn varied from 0.003 to 0.067 mg kg⁻¹ and from 43.9 to 137 mg kg⁻¹, respectively, and exhibited positive correlations with the ²³⁹⁺²⁴⁰Pu activity both in the surface sediments (0–1 cm) and upper layers (7 cm) of the sediment cores. Therefore, by using Pu as a tracer, we estimated that the oceanic input contributed 2.0 tons y⁻¹ of Hg and 1.0 × 10³ tons y⁻¹ of Zn to the SYS sediments between 2011 and 2014, which accounted for 33% and 3% of total buried Hg and Zn, respectively. These findings indicate that environmental pollution control should also consider the oceanic contribution of some pollutants. The results of the present work help to elucidate the biogeochemical cycling of trace metals in marginal seas, and are helpful for managing environmental pollution in marine environments.

Photovoltaic industry has shown tremendous growth among renewable energy sector. Though, this high installation rate will eventually result in generation of large volume of end-of-life photovoltaic waste with hazardous metals. In present study, reported leached metal contents from different photovoltaics in previous investigations were utilized for (i) potential fate and transport analysis to soil and groundwater and, (ii) estimating ecological and human health risks via dermal and ingestion pathways for child and adult sub-populations. The results indicate that the children are at highest risk, mainly due to lead (hazard quotient from 1.2 to 2.6). Metals, such as cadmium, lead, indium, molybdenum and tellurium pose maximum risks for child and adult sub-populations via soil-dermal pathway followed by soil-ingestion pathway. This is further proved by calculated high values of contamination factor and geo-accumulation index for cadmium (102.4), indium (238.9) and molybdenum (16.12). The estimated soil contamination is significant with respect to aluminium, silver, cadmium, iron, lead, however, groundwater contamination was insignificant. Exposure to polluted soils yields an aggregate hazard index (for non-cancer effects) > 1 for all four pathways, with soil dermal pathway as the major contributor. Lead poses significant cancer risk for all scenarios (average risk: 0.0098 to 0.047 (soil) and 2.1 × 10⁻⁵ to 3.5 × 10⁻⁵ (groundwater)), whereas acceptable non-cancer risk was observed for other metals from groundwater exposure. Further, variance contribution and spearman correlation coefficient analysis show that metal concentration, exposure frequency and ingestion rate are the main contributors towards overall uncertainty in risk estimates. More detailed assessment for environmentally-sensitive metals should be carried out by considering other field breakage scenarios also, although the assessment suggests low risk for majority of metals examined.

Per- and polyfluoroalkyl substances (PFASs) have been used for decades within industrial processes and consumer products, resulting in frequent detection within the environment. Using zebrafish embryos, we screened 38 PFASs for developmental toxicity and revealed that perfluorooctanesulfonamide (PFOSA) was the most potent developmental toxicant, resulting in elevated mortality and developmental abnormalities following exposure from 6 to 24 h post fertilization (hpf) and 6 to 72 hpf. PFOSA resulted in a concentration-dependent increase in mortality and abnormalities, with surviving embryos exhibiting a >12-h delay in development at 24 hpf. Exposures initiated at 0.75 hpf also resulted in a concentration-dependent delay in epiboly, although these effects were not driven by a specific sensitive window of development. We relied on mRNA-sequencing to identify the potential association of PFOSA-induced developmental delays with impacts on the embryonic transcriptome. Relative to stage-matched vehicle controls, these data revealed that pathways related to hepatotoxicity and lipid transport were disrupted in embryos exposed to PFOSA from 0.75 to 14 hpf and 0.75 to 24 hpf. Therefore, we measured liver area as well as neutral lipids in 128-hpf embryos exposed to vehicle (0.1% DMSO) or PFOSA from 0.75 to 24 hpf and clean water from 24 to 128 hpf, and showed that PFOSA exposure from 0.75 to 24 hpf resulted in a decrease in liver area and increase in yolk sac neutral lipids at 128 hpf. Overall, our findings show that early exposure to PFOSA adversely impacts embryogenesis, an effect that may lead to altered lipid transport and liver development.

of bioaugmentation strategies are an obstacle for damage mitigation caused by oil spills in marine environments. Cells added to the contaminated sites are quickly lost by low adherence to the contaminants, rendering ineffective. This study used two hydrocarbonoclastic species - Rhodococcus rhodochrous TRN7 and Nocardia farcinica TRH1 cells - growing in mineral medium containing hexadecane to evaluate cell distribution in a crude-oil contaminated marine water. Cell affinity to hydrophobic compounds was quantified using Microbial Adhesion to Hydrocarbons test and analysis of fatty acids profile was performed using the Microbial Identification System. Bioremediation simulations were set up and cell populations of both strains were quantified by Fluorescent in situ Hybridization. R. rhodochrous and N. farcinica reached up to 97% and 60% of adhesion to hexadecane, respectively. The carbon source had more influence on the fatty acid profiles of both strains than the microbial species. The presence of 45.24% of 13:0 anteiso on total fatty acids in R. rhodochrous and 12.35% of saturated fatty acids with less than 13 carbons atoms in N. farcinica, as well as the occurrence of fatty alcohols only in presence of hexadecane in both species, are indicators that fatty acid changes are involved in the adaptation of the cells to remain at the water/oil interface. Cell quantification after bioremediation simulations revealed an increase in the density of both species, suggesting that the bioremediation strategies resulted on the increase of hydrocarbonoclastic species and up to 27.9% of all prokaryotic microbial populations in the microcosms were composed of R. rhodochrous or N. farcinica. These findings show the potential of application of these two bacterial strains in bioaugmentation of hydrocarbon-contaminated marine ecosystems.R. rhodochrous TRN7 and N. farcinica TRH1 hydrocarbonoclastic strains modify the fatty acid profile and increases density, optimizing hydrocarbons biodegradation.

In steroidogenic cells, steroids are synthesized de novo from cholesterol stored in lipid droplets (LDs). The size of LDs regulated by adipose differentiation-related protein (ADRP) is closely related to cholesterol ester hydrolysis. Many studies reported that cadmium (Cd) had dual effects on steroidogenesis in granulosa cells (GCs). However, the role of LD and its regulation in abnormal steroidogenesis caused by Cd exposure remain unknown. In current study, female rats were exposed to CdCl₂ during gestation and lactation, and influence of such exposure was investigated in ovarian GCs of female offspring. The size of LDs was found much smaller than normal in GCs; ADRP was down-regulated and hormone-sensitive lipase (HSL) phosphorylation was increased, followed by up-regulation of steroidogenic acute regulatory protein (StAR) and cholesterol side-chain cleavage enzyme (CYP11A1); the expression of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 (PLCβ2) and protein kinase C alpha type (PKCα) were both decreased accompanying the ADRP down-regulation. This series of events resulted in a high level of progesterone in serum. Similar results were demonstrated in GCs treated with 20 μM CdCl₂ for 24 h in vitro. The protein level of ADRP was decreased after gene silencing of PLCβ2/PKCα, and the knockdown of PLCβ2/PKCα/ADRP led to micro-sized LD formation. We found that Cd exposure down-regulated ADRP by inhibiting the PLCβ2-PKCα signaling pathway, reduced the size of LDs, and promoted HSL phosphorylation. StAR and CYP11A1 were both up-regulated following the hydrolysis of cholesterol ester, which led to a high production of progesterone. LD thereby is a target subcellular organelle for Cd to affect steroid hormone synthesis in ovarian GCs. These findings might help to uncover the mechanism of ovarian dysfunction and precocious puberty caused by Cd pollution.

The imprudent agricultural practices are leading to an increasing load of pesticides in agricultural fields. Thus, there is a need to minimize the harmful effect of pesticides by adopting sustainable strategies. In the recent past decade, kinetin, a plant synthetic hormone, has been reported as a pesticide toxicity alleviator in higher plants. But its role in mitigating pesticide toxicity in cyanobacteria is still limited. Thus, in current study an attempt has been made to investigate the potential of kinetin in regulating cypermethrin, an insecticide, induced toxicity in Anabaena PCC 7120 and Nostoc muscorum ATCC 27893. Cypermethrin (Cyp₁; 2 μg ml⁻¹ and Cyp₂; 4 μg ml⁻¹) showed negative impact on growth, photosynthetic pigments, photosynthetic O₂-evolution and primary photochemistry of PS II (Phi_P₀, Psi_₀, Phi_E₀) resulting in decrease in performance index (PIABS). However, under similar conditions, increases in energy flux parameters (ABS/RC, TR₀/RC, ET₀/RC and DI₀/RC) were noticed. Cypermethrin at both the doses enhanced the level of oxidative stress biomarkers (SOR, H₂O₂, and MDA equivalent contents) despite of increased antioxidant enzymatic activity (SOD, POD, CAT and GST).Under similar condition, cypermethrin at tested doses caused substantial decrease in non-enzymatic antioxidant contents (proline, cysteine and NP-SH). Nevertheless, kinetin treatment attenuated cypermethrin induced oxidative stress by further up-regulating the activity of enzymatic antioxidants and by enhancing the contents of non-enzymatic antioxidants. Thus, with the application of kinetin improved photochemistry of PS II and growth yield of both the cyanobacteria were observed even in the presence of cypermethrin. Current results establish that cypermethrin induces toxicity on photosynthesis, photosynthetic pigments and growth, and this effect was more pronounced in Anabaena PCC 7120 than Nostoc muscorum ATCC 27893. Furthermore, the potential role of kinetin in mitigating the toxicity of cypermethrin in both the cyanobacteria provides an insight to be used in paddy fields for sustainable agricultural practices.