Both amphibian metamorphosis assay (AMA) and larval amphibian growth and development assay (LAGDA) can detect thyroid-mediated modality and adversity on the basis of morphological changes during the thyroid hormone-dependent metamorphosis. They are used for identification of thyroid hormone system disrupting chemicals (TDCs) for non-target organisms or the environment. The EU Guidance recommends that the AMA and the LAGDA should be used to address sufficient investigation of the thyroid-mediated modality and adversity, respectively. In the EU discussions over identification of TDCs, the necessity of using LAGDA as a follow-up of positive results of the AMA has been questioned because of the overlap between the endpoints and the exposure of both tests. This study analyzed similarities, differences, and sensitivity of these two assays in detection of TDCs. For agonists and most of antagonists of the hypothalamic-pituitary-thyroid (HPT) axis, both AMA and LAGDA can detect the thyroid-mediated modality and adversity. The LAGDA, as a follow-up of the positive results of the AMA, may not be needed because the results of AMA are considered enough for identification of TDCs. For chemicals like inhibitors of iodotyrosine deiodinase, the LAGDA is considered necessary for identification of TDCs because the thyroid-mediated adversity cannot be detected until Nieuwkoop and Faber (NF) stage 62. Incorporation of mechanistic endpoints into existing test guidelines and the use of Xenopus Eleutheroembryo Thyroid Assay (XETA), extended amphibian metamorphosis assay (EAMA) and adverse outcome pathways (AOPs) for testing and identification of TDCs are further discussed.

Potential nuclear accidents propel serious environmental pollution, and the resultant radionuclide release devastates severely the environment severely and threatens aquatic organism survival. Likewise, ongoing climate change coupled with the gradual increase in global surface temperatures can also adversely impact the aquatic ecosystems. In the present study, we preconditioned zebrafish (Danio rerio) at three different temperatures (18 °C, 26 °C and 34 °C) to investigate the effects of a temperature profile on their radiosensitivity (exposure to 20 Gy of gamma rays) to identify the potential biochemical mechanism responsible for influencing radiosensitivity. We found that preconditioning of zebrafish at different temperatures moulded specific gut microbiota configurations and impacted hepatic glycometabolism and sensitivity to subsequent radiation. Following antibiotic treatment to reduce gut bacteria, these observed differences in the expression of hepatic glycometabolism-related genes and radiation-induced intestinal toxicity were minimal, supporting the hypothesis that the gut bacteria reshaped by different ambient temperatures might be the key modulators of hepatic functions and radiosensitivity in zebrafish. Together, our findings provide novel insights into the connection of radiation injuries with temperature alterations in fish, and suggest that maintaining the stability of gram-positive bacteria may be efficacious to protect aquatic organisms against short or long-term radioactive contamination in the context of global climate change.

Monitoring beach plastic contamination across space and time is necessary for understanding its sources and ecological effects, and for guiding mitigation. This is logistically and financially challenging, especially for microplastics. Citizen science represents an option for sampling accessible sites to support long term monitoring, but challenges persist around data validation. Here we test a simple citizen science methodology to monitor visible microplastic contamination on sandy beaches using a standard quadrat unit (50 cm × 50 cm x 5 cm depth) sieved to 1 mm, to support the analysis of microplastic on two islands within the marine protected area of the Galápagos Archipelago, Ecuador (San Cristóbal and Santa Cruz islands). High school and university students undertook supervised sampling of two beaches in 2019–2020 collecting over 7000 particles. A sub-sample of the suspected microplastics collected (n = 2,213, ∼30% total) were analysed using FTIR spectrometry, confirming 93% of particles >1 mm visually identified by students were microplastics or rubber, validating this method as a crowd-sourced indicator for microplastic contamination. These data provide important insights into the plastic contamination of Galápagos, revealing plastic abundances of 0–2524 particles m⁻² over the two beaches (the highest reported in Galápagos). Strong accumulation gradients were measured parallel to the waterline at Punta Pitt (San Cristobal island) and perpendicular to the waterline at Tortuga Bay (Santa Cruz island), where four-fold higher concentrations were recorded at the sea turtle nesting habitat on the back-beach dune. No significant seasonal trends were measured during one year. These results demonstrate the value of citizen science in filling spatiotemporal knowledge gaps of beach contamination to support intervention design and conservation.

In this study, the occurrence of pharmaceuticals, hormones and bacterial community structures was studied at a wastewater treatment plant in Finland having two different parallel treatment lines: conventional activated sludge (CAS) treatment with a sedimentation stage, and a membrane bioreactor (MBR). Influent and effluents were sampled seven times over a period of one year. The bacterial communities of the influent samples showed a high degree of similarity, except for the February sample which had substantially lower diversity. There was significant fluctuation in the species richness and diversity of the effluent samples, although both effluents showed a similar trend. A marked decrease in diversity was observed in effluents collected between August and November. The initiation of nitrogen removal as a result of an increase in temperature could explain the changes in microbial community structures. In overall terms, suspended solids, bacteria and total organic matter (COD and BOD) were removed to a greater extent using the MBR, while higher Tot-N, Tot-P and nitrate removal rates were achieved using the CAS treatment. Estrone (E1) concentrations were also consistently at a lower level in the MBR effluents (<0.1–0.68 ng/l) compared to the CAS effluents (1.1–12 ng/l). Due to the high variation in the concentrations of pharmaceuticals, no clear superiority of either process could be demonstrated with certainty. The study highlights the importance of long-term sampling campaigns to detect variations effectively.

Triclocarban (TCC), a bactericide widely used in personal care products, is frequently detected in soil and surface water, which may affect the environmental behavior of other environmental pollutants by changing the community structure of environmental microorganisms. This work evaluated the effects of TCC on the degradation and migration of seven herbicides and five fungicides in soil under co-occurrence conditions. TCC significantly increased the persistence of the pesticides in soil, and this effect increased with TCC concentration. For example, the half-life of metolachlor, atrazine, metribuzin, and metamitron increased 44%, 38%, 153%, and 33%, respectively, with 10 mg/kg TCC and increased 60%–640% with 100 mg/kg TCC. After 90 days, the residue of the pesticides in soil treated with TCC was significantly elevated relative to the control. TCC treatment could also increase the potential leaching risk of the herbicides in the soil, as indicated by an increased Groundwater Ubiquity Score (GUS) index. The reduced abundance of soil bacteria by TCC might be an essential reason for the impacts on the environmental behavior of the pesticides. This study confirmed that TCC could slow down the degradation of pesticides in soil, increase their persistence and even affect the leaching behavior, thus influencing the risks of the pesticides to the environment.

The development of saline-alkali lands has contributed to the increasing discharge of alkaline salt-laden wastewater, which poses a threat to aquatic organisms. However, the comprehensive effect of alkaline salt on Microcystis aeruginosa, a harmful cyanobacterium, remains unclear. In this study, the growth, physiology, cell ultrastructure and production of microcystin-LR (MC-LR) in Microcystis aeruginosa exposed to four levels of alkaline salt stress were evaluated. The growth of Microcystis aeruginosa was stimulated at an electrical conductivity (EC) of 2.5 mS/cm compared to the control, as supported by the increased cell density, photosynthetic pigment and protein contents. Microcystis aeruginosa could tolerate a certain level of alkaline salt (i.e., EC of 5 mS/cm) via increasing photosynthetic pigment contents to protect cells from alkaline salt stress, but the antioxidant defence system and cell ultrastructure were not affected. When EC increased to 7.5 mS/cm, alkaline salt caused oxidative stress and toxicity in Microcystis aeruginosa, as evidenced by analysis of the integrated biomarker response (IBR). Furthermore, the photosynthetic pigment and protein contents decreased, and cell apoptosis associated with ultrastructural changes was observed. Therefore, we propose that EC of 7.5 mS/cm is a threshold for growth of Microcystis aeruginosa. Additionally, the intracellular MC-LR content was stimulated by alkaline salt, and the highest value was observed at EC of 2.5 mS/cm. The extracellular MC-LR content increased with the increasing alkaline salt concentration. When EC was 7.5 mS/cm, the extracellular MC-LR content was significantly higher than in the control and was associated with the upregulated mcyH gene. This study recommends that more attention should be paid to the risk of Microcystis aeruginosa bloom and microcystin-LR pollution in lakes located in salinization regions.

Estuaries are one of the most valuable biomes on earth. Although humans are highly dependent on these ecosystems, anthropogenic activities have impacted estuaries worldwide, altering their ecological functions and ability to provide a variety of important ecosystem services. Many anthropogenic stressors combine to affect the soft sedimentary habitats that dominate estuarine ecosystems. Now, due to climate change, estuaries and other marine areas might be increasingly exposed to the emerging threat of megafires. Here, by sampling estuaries before and after a megafire, we describe impacts of wildfires on estuarine benthic habitats and justify why megafires are a new and concerning threat to coastal ecosystems. We (1) show that wildfires change the fundamental characteristics of estuarine benthic habitat, (2) identify the factors (burnt intensity and proximity to water's edge) that influence the consequences of fires on estuaries, and (3) identify relevant indicators of wildfire impact: metals, nutrients, and pyrogenic carbon. We then discuss how fires can impact estuaries globally, regardless of local variability and differences in catchment. In the first empirical assessment of the impact of wildfires on estuarine condition, our results highlight indicators that may assist waterway managers to empirically detect wildfire impacts in estuaries and identify catchment factors that should be included in fire risk assessments for estuaries. Overall, this study highlights the importance of considering fire threats in current and future estuarine and coastal management.

Sixteen paired surface sediment samples (0–5 cm, n = 32) covering upstream to downstream of water-level-fluctuation zone of Three Gorges Reservoir, China were collected in March 2018 (following six months of submergence) and September 2018 (after six months of exposure). Seventeen per- and poly-fluoroalkyl substances (PFASs) were quantified to evaluate contamination characteristics, apportion source categories and estimate mass inventory and loadings. The concentration of ΣPFASs ranged from 0.26 to 0.82 ng·g⁻¹ at high water-level (HWL) and 0.46–1.53 ng·g⁻¹ at low water-level (LWL). Perfluorooctanoic acid (PFOA, mean: 0.32 ng·g⁻¹) and perfluorooctane sulfonate (PFOS, mean: 0.12 ng·g⁻¹) dominated, accounting 44.9% and 16.3% of the total PFASs, respectively. The distribution of PFASs was more influenced by anthropogenic activities than physicochemical parameters of the sediments. Positive matrix factorization (PMF) identified PFOA-based products was the major sources (40.1% and 38.6%, respectively). Besides, the direct sources of PFOA-, PFOS-, PFNA-and PFBA-based products played the predominant role, while the indirect degradation of precursors contributed relatively little. The sediment (0–5 cm) mass inventory of PFASs at LWL (57.5 kg) was higher than HWL (39.3 kg). The annual mass loadings of the total PFASs, PFOA, PFOS, perfluoroundecanoic acid (PFUdA) and perfluorononanoic acid (PFNA) from the upstream to the middle-lower reaches of Yangtze River were 27.4 kg, 11.1 kg, 4.63 kg, 2.89 kg and 2.57 kg, respectively. This study could provide the basic datasets of PFASs in surface sediments of the TGR, and also indicate an important transport of PFASs from upstream to the lower reaches, which should be further studied as well.

The willingness of migrating due to air pollution is widespread in China. However, there is a lack of direct evidence and discussion regarding whether this willingness has been translated into action. In this study, PM2.5 concentrations were used to represent air pollution in each city and were compared with individual migration data from the China Labor-force Dynamics Survey (CLDS) to examine population migration effects caused by air pollution. This study showed that (1) Population migration between Chinese cities shows sensitivity to air pollution, and air pollution increases the probability of moving away for local population. This finding is held under multiple robustness and endogeneity tests. (2) Population migration effects caused by air pollution were more pronounced among women, middle-aged people, those with lower educational levels, from agricultural households, Han Chinese groups, and populations in southern cities. (3) The use of individual self-rated health data verified that physical health is an important channel through which individual migration decisions are influenced by air pollution, the older an individual, the more his or her health was affected. In light of these findings, this study led to conclusions regarding targeted policy recommendations in terms of talent clustering, social equity, and demographic balance.

Many communities around the country are undergoing contentious battles over the installation of artificial turf. Opponents are concerned about exposure to hazardous chemicals leaching from the crumb rubber cushioning fill made of recycled tires, the plastic carpet, and other synthetic components. Numerous studies have shown that chemicals identified in artificial turf, including polycyclic aromatic hydrocarbons (PAHs), phthalates, and per- and polyfluoroalkyl substances (PFAS), are known carcinogens, neurotoxicants, mutagens, and endocrine disruptors. However, few studies have looked directly at health outcomes of exposure to these chemicals in the context of artificial turf. Ecotoxicology studies in invertebrates exposed to crumb rubber have identified risks to organisms whose habitats have been contaminated by artificial turf. Chicken eggs injected with crumb rubber leachate also showed impaired development and endocrine disruption. The only human epidemiology studies conducted related to artificial turf have been highly limited in design, focusing on cancer incidence. In addition, government agencies have begun their own risk assessment studies to aid community decisions. Additional studies in in vitro and in vivo translational models, ecotoxicological systems, and human epidemiology are strongly needed to consider exposure from both field use and runoff, components other than crumb rubber, sensitive windows of development, and additional physiological endpoints. Identification of potential health effects from exposures due to spending time at artificial turf fields and adjacent environments that may be contaminated by runoff will aid in risk assessment and community decision making on the use of artificial turf.