As typical submerged aquatic vegetation, Ruppia species are facing population reductions due to anthropogenic impacts. In this study, we investigated the effects of temperature and salinity on seed germination and seedling establishment of Ruppia sinensis seeds collected from northern China. The effects of seven salinities (0–50) and six water temperatures (0–30°C) on seed germination were investigated to identify the environmental conditions that could potentially limit survival and growth. We found that: 1) optimum seed germination was salinity 5 at 30°C; 2) high salinity (salinity 40–50) and low temperature (0°C) significantly inhibited seed germination; 3) seed germination with increasing temperature showed a bimodal pattern at suitable salinities (5–10); 4) storing seeds at high salinities (40–50) or low temperature (0°C) promoted germination after transferal to optimal germination conditions. These findings may serve as useful information for R. sinensis habitat establishment and restoration programs.

Hydrocarbon degradation (HD) potential by autochthonous microorganisms in the coastal sediments of the NW area of Portugal (coastal sandy beaches and estuaries of the rivers Minho and Douro) was evaluated, investigating if water and sediment contamination/characteristics influence it. Sediments were characterized for microbial abundance (by DAPI), HD microorganisms' abundance (by MPN), microbial community structure (by ARISA), hydrocarbons (by FTIR and SPME-GC–MS), hazardous and noxious substances (SPME-GC–MS) and metals (by AAS). To our knowledge, this is the first time all these pollutants, including the selected HNS, were measured simultaneously in sediments of the selected coastal area. Higher contaminants concentrations were, generally, registered in Douro samples. A clear differentiation of the microbial community structure between beaches and estuaries was observed, as well as, between Douro and Minho river estuaries. BIO-ENV analysis indicated both sediment characteristics (e.g. OM content) and contaminants presence/concentrations (e.g. tetrachloroethylene presence) affected the structure of the microbial community along the studied areas. In all the selected sites, the characterized autochthonous microbial communities showed potential for hydrocarbons degradation, with HD microorganisms being found in all collected sediments. These microorganisms can be a valuable asset to recover contaminated areas, but sediment characteristics and contaminants presence/levels need to be taken into account as they can affect their bioremediation potential and the success of their application as biotechnological tool.

Little is known about the dynamics of particulate inorganic and organic matter in brackish water controlled by tidal prism and seasonal river discharges in Macuse estuary, on central Mozambique coast, southern Africa. This manuscript illustrates seasonal variation of biogeochemical flow dynamics in a tropical estuarine system in Zambézia province. The data were collected at 42 stations, including tidal current measurements with an Acoustic Doppler Current Profiler, tidal elevation with a tidal-gauge and nitrate, silicate (SiO2), dissolved oxygen and chlorophyll-a with a photometer device system and tidal currents with multisensory instrument device (Current, Temperature and Depth device). These field data helped to calibrate a twelve months simulation of a 3D Computational Aquatic Ecosystem Dynamics Model (3D CAEDM) in 2014. The results emphasize a tidal elevation of 4-m height that generated tidal currents of 120 cm/s. They combination with seasonal runoff of ~500 m3/s from Namacurra River and averaged bathymetry morphology of 10-m depth, led on to the seasonal concentration flux of dissolved oxygen, nitrate, SiO2 and chlorophyll-a during the ebbing and flooding. In addition, the river discharges were found to be the main source of nitrate and SiO2, while the tidal prism led on the hydrological flow of the brackish-waters during the rainy season with maximum salinity anomaly of 16%. Besides the data obtained, the 3D model matches in reproducing the biogeochemical data flow have considerable potential possess of a valuable information in long- and short-term and large- and small-scale that may allow policy-makers to determine political decisions for bio-conservation, sustainability and coastal management.

Given scarcity of knowledge on gender ecophysiological responses of tropical marine organisms to global climate change, the major aim of this research was to investigate potential sex differences in oxidative status of topshell Trochus histrio, after a combined exposure to increased temperature and pCO2. Lipid peroxidation, heat-shock response and antioxidant enzymatic activities were evaluated. Lipid peroxidation varied differently between sexes, with males undergoing cellular damage under high pCO2, which was elevated temperature-counteracted. Heat shock response was thermo- and sex-regulated, with males exhibiting significantly higher heat shock proteins production than females. Catalase activity increased with temperature and was exacerbated in combination with hypercapnia, being highest in females, while glutathione S-transferases activity peaked in males. These results clearly support the existence of distinct physiological strategies to cope oxidative stress between sexes, apparently more efficient in females, and also reinforce for the need of encompassing sex as meaningful variable in future biomarker studies.

To elucidate the effects of the herbicide Irgarol 1051 on the gene expression of heat shock protein 90 (HSP90) in hard corals, we isolated a full-length cDNA encoding HSP90 from Acropora tenuis, which has a deduced open reading frame of 732-amino acid residues with a predicted molecular mass of 84.5 kDa. The amino acid sequence of A. tenuis HSP90 showed a high degree of similarity with the hermatypic-coral HSP90 families. After a 7-d exposure to 1 or 10 μg/L of Irgarol, the body colours of corals in the 10 μg/L treatment group were significantly whiter (bleached), whereas no such effects were observed in the corals in the 1 μg/L treatment group. However, the expression level of coral HSP90 was significantly downregulated after exposure to both 1 and 10 μg/L Irgarol. These results suggest that A. tenuis HSP90 may be a useful molecular biomarker to predict bleaching caused by herbicides.

This paper explores the governance characteristics of marine plastic debris, some of the factors underpinning its severity, and examines the possibility of harnessing corporate social responsibility (CSR) to manage plastic use within the contextual attitudes of a contemporary global society. It argues that international and domestic law alone are insufficient to resolve the “wicked problem” of marine plastic debris, and investigates the potential of the private sector, through the philosophy of CSR, to assist in reducing the amount and impacts of marine plastic debris. To illustrate how CSR could minimise marine plastic pollution, an industry-targeted code of conduct was developed. Applying CSR would be most effective if implemented in conjunction with facilitating governance frameworks, such as supportive governmental regulation and non-governmental partnerships. This study maintains that management policies must be inclusive of all stakeholders if they are to match the scale and severity of the marine plastic debris issue.

The effects of enclosed, semi-closed, and opened reclamation methods on the macrobenthos community structure were investigated. Compared with their paired controls, water salinity decreased sharply in the enclosed reclamation region with no apparent change in the opened reclamation region. Declining species and biodiversity was observed in the reclamation regions, but the extent of this declining trend was weaker in the semi-closed and opened reclamations than in the enclosed reclamation region. The ABC curve indicated that the enclosed reclamation was disturbed, whereas the semi-closed and opened reclamations were undisturbed. Taken together, these results suggest that reclamation may have a negative effect on the community and health status of macrobenthos in the intertidal wetlands of the Yangtze Estuary. Semi-closed and opened reclamation methods may mitigate the problem of a salinity decrease caused by enclosed reclamation, while also having a relatively weaker negative effect on community structure and wetland habitat.

Poor microbial water quality jeopardizes the health and safety of food produced by aquaculture farms. Three fish farms and transect sites in Singapore were assessed for microbial water quality and antimicrobial resistance determinants. Of the 33 multidrug resistant E. coli isolated from surface waters of the Johor Straits, 81.8% were ESBL producers. The relative abundance of sul1, qnrA and intI1 genes were higher in sediments than surface waters. Among the surface water samples, higher concentrations (10−1−101) of beta-lactamases (blaSHV, blaOXA, blaCTX-M) were detected in the transect sites. This study highlights a potential antimicrobial resistance transmission chain from environmental waters, to animal carriers and humans.

Proteome response of plants is an important process that enables them to cope with environmental stress including metal stress. In this study, the proteome of Gracilaria lemaneiformis exposed to lead was investigated. Two-dimensional gel electrophoresis analysis revealed 123 protein spots, among which 14 proteins were significantly differentially expressed and identified using MALDI-TOF MS. Two of the up-regulated proteins were identified and predicted to be involved in photosynthesis and signal transduction, while eleven down-regulated proteins were functionally grouped into five classes including photosynthesis, energy metabolism, protein metabolism, carbohydrate transport and metabolism, and antioxidation proteins. There was also an up-regulation in superoxide dismutase, peroxidase, glutathione s-transferase, and heat-shock protein 70 upon Pb exposure. Proteomic studies provide a better picture of protein networks and metabolic pathways primarily involved in intracellular detoxification and defense mechanisms.

This work tackles a multidisciplinary study on the recent sedimentary record of the Bilbao estuary (northern Spain), which is the backbone of a city that was primarily industrial and now is widely recognized as a successful example of urban transformation. Although hotspots of heavily polluted materials still remain at the mouth of the two main tributaries (Galindo and Gobelas), the data obtained confirm the ongoing formation of a new layer of sediments (here called “postindustrial zone”) covering historically polluted and azoic deposits. It is characterized by largely variable levels of metals and magnetic susceptibility and moderate-to-high abundances of benthic foraminifera. Monitoring of the evolution of this layer appears a key factor to assess environmental improvement and decision-making in polluted estuaries.