Microbial communities in wetland soils play vital roles in biogeochemical cycling of nutrients. In this study, the soil samples were collected from Suaeda, reed and Suaeda-reed hybrid zones in Shuangtaizi River Estuary, Northeast China, and the rhizosphere bacterial communities were compared using Illumina MiSeq sequencing. The microbial richness, diversity and structure of bacterial communities varied greatly in reed and Suaeda. Canonical correspondence analysis and Mantel test indicated that pH was the most significant factor (P < 0.05) in bacterial community assembly. Proteobacteria was the most dominant phylum, accounting for 45.7–58.0% of the total sequences. Thioprofundum, Thiohalomonas and Exiguobacterium were the predominant genera in Suaeda, while Exiguobacterium, Gillisia, Desulfomonile, Citrobacter, Thioprofundum and Acinetobacter were the core species in reed. PICRUSt analysis revealed similar functional profiles of rhizosphere microbiota in reed and Suaeda. Nitrate reduction related genes were abundant for nitrogen metabolism, whereas assimilatory sulfate reduction was the major process for sulfur metabolism.

Recent extreme hydrological events, such as high rainfall and storms, have been linked to climate change worldwide due to their relatively uncommon trends, which are generally described as having varying frequencies and higher intensities. Similar extreme rainfall trends were observed in the northwest Arabian Gulf during the 2018–2019 season. Heavy rainfall events have triggered high discharges in the river of Shatt Al-Arab, where, prior to these events, the discharge rate has exhibited a declining trend over the past 40 years. Shatt Al-Arab provides various physical, chemical, and biological functions in the region. In this study, some recent and extensive measurements of salinity and post-processed satellite images of chlorophyll-a in the northwest Arabian Gulf were recorded to assess the response of the region to the higher discharges. The results indicated that the salinity decreased significantly during and after the rainfall events in response to the higher discharges from Shatt Al-Arab. The effects of higher discharges extended over most of Kuwait's coastline, including Kuwait Bay. The chlorophyll-a distributions doubled after the extreme events, emphasising the effects of Shatt Al-Arab. Collectively, the results indicated that the discharge from Shatt Al-Arab during the extreme event exceeded 1400 m³/s, while, in the previous seasons, it rarely exceeded 80 m³/s. The higher discharge levels of discharge would result in various changes in ecology at multiple levels due to the sudden and reversing shift in the hydro-environmental processes.

Cyst abundance and identity are essential for understanding and predicting blooms, and for assessing the dispersal of toxic target dinoflagellate species by natural or human mediated ways, as with ballast waters. The aim of this study was to apply rapid, specific and sensitive qPCR assays to enumerate toxic dinoflagellate cysts in sediment samples collected from Adriatic harbours. The molecular standard curves of various target species allowed obtaining the rDNA copy number per cyst. The analytical sensitivity for specific standard curves was determined to be 2 or 10 rDNA copies per reaction. The abundance varied in the range of 1–747 dinoflagellate cysts g⁻¹ dry weight. The assays showed greater sensitivity as compared to counts by light microscopy. This qPCR method revealed a powerful tool for the quantification of cysts from toxic dinoflagellate resting stages in sediment samples from Adriatic ports.

Marine litter is a global, persistent, and increasing threat to the oceans, and numerous initiatives aim to address this challenge. Fishing For Litter (FFL) is a voluntary clean-up scheme, where litter is collected as part of routine fishing operations. We surveyed fishers (n = 97) and stakeholders (n = 22) in the UK to investigate perceptions of FFL, its strengths and weaknesses, and potential co-benefits of the scheme. Fishers reported being aware of and concerned about the negative impacts of litter. Overall, FFL was evaluated very positively (7.85/10). In addition, FFL fishers reported less environmentally harmful waste management behaviors both out at sea and in other contexts than did non-FFL fishers. Fishers and stakeholders listed strengths and weaknesses of the scheme and made suggestions for future changes. As well as directly helping to remove litter, this paper demonstrates that clean-up schemes can make a contribution to addressing the underlying causes of marine pollution.

The distribution, composition, potential sources and ecological risk of 16 US EPA priority polycyclic aromatic hydrocarbons (PAHs) were investigated in surface water from coastal Bohai Bay. The PAH concentrations ranged from 48.0 to 607 ng L⁻¹ with the mean value of 267 ng L⁻¹ in dissolved phase and 198 to 935 ng L⁻¹ with the mean value of 424 ng L⁻¹ in particle-associated phase, and decreased following the order: Qinhuangdao (QHD) > Caofeidian (CFD) > Huanghuagang (HHG). The 2–3 ring PAHs accounted for 79.9% to 86.2% in particle-associated phase and 65.6% to 73.2% in dissolved phase of total 16 PAHs, in which naphthalene (Nap) was the most dominant compound. The PAHs in surface seawater mainly originated from petrogenic sources according to the molecular ratios of benzo[a]anthracene/228 and fluoranthene/202. Risk quotients indicated that there was potential ecological risk for PAHs in surface seawater, especially for pyrene and fluorene.

To examine whether historical fish bones can record the magnitude of heavy metal pollution, we analyzed up to 700 years old fish bone remains extracted from an ornithogenic sediment profile on Nandao Island, South China Sea. Bulk sediments and subfossil fish bones were analyzed for elemental and mineralogical composition, as well as stable carbon and nitrogen isotopes. The results showed that pre-1850 CE fish bones experienced significant diagenesis, and could not be used to reconstruct historical record of heavy metal pollution. Fish bone diagenesis was mainly attributed to the erosion from guano in sediment profile. In contrast, the fish bones from in post-1850 CE time were well preserved and could provide useful information on historical pollution loads over the past 160 years. Since 1850 CE, relatively high concentrations of heavy metals from anthropogenic sources, especially Zn, were recorded in fish bone subfossils on Nandao Island, South China Sea.

The strategic location of Malaysia along the world's busiest trade waterways underscores the need to cope ballast water issues for both domestic and international shipping. The adoption of Ballast Water Management Convention 2004 (BWMC) by the International Maritime Organization is suitable for management plans intended to prevent the introduction of invasive species through ballast water discharge. Malaysia has ratified the BWMC in September 2010 and the Convention has come into force in September 2017. However up to now, the BWMC has not been fully implemented by Malaysia for ships operating in its waters. This paper analyse the headway in implementing the provisions of the BWMC in Malaysia as well as the issues and challenges encountered for the implementation. The paper concludes that Malaysian government should promulgate laws and policies to clearly communicate on ballast water issues to the shipping industry communities.

Past major oil spill disasters, such as the Prestige or the Deepwater Horizon accidents, have shown that spilled oil may drift across the ocean for months before being controlled or reaching the coast. However, existing oil spill modelling systems can only provide short-term trajectory simulations, being limited by the typical met-ocean forecast time coverage. In this paper, we propose a methodology for mid-long term (1–6 months) probabilistic predictions of oil spill trajectories, based on a combination of data mining techniques, statistical pattern modelling and probabilistic Lagrangian simulations. Its main features are logistic regression modelling of wind and current patterns and a probabilistic trajectory map simulation. The proposed technique is applied to simulate the trajectory of drifting buoys deployed during the Prestige accident in the Bay of Biscay. The benefits of the proposed methodology with respect to existing oil spill statistical simulation techniques are analysed.

In this study, the fate of residual oils was investigated during remediation activities for a year after the Wu Yi San oil spill. Microscope observations showed that relatively large amounts of oil-suspended particulate matter aggregate (OSA) were formed. Negatively buoyant OSA was the dominant form (>95%), followed by neutrally (~5%) and positively buoyant (<1%) forms. To elucidate the dominance of negative buoyancy OSA, physicochemical properties of the mineral and residual oils were identified. Chemical analysis showed that the weathering percentage of residual oils was 43.7 ± 2.59%, which was the driving factor for sedimentation of OSA. As the density of oil increased with weathering stages, the density of OSA also increased simultaneously. These results showed that, during mechanical remediation activities, resurfaced residual oils can form negative buoyancy OSA which tends to sink and transfers oil contaminants from intertidal to benthic environments.

Research on marine microplastics continues to increase in popularity, with a large number of studies being published every year. However, with this plethora of research comes the need for a standardised approach to quantification and analysis procedures in order to produce comparative assessments. Using data collected from neuston nets in 2016, parameters for quantifying microplastics were compared. Surface area was the most accurate parameter to describe plastic size and should be used to describe plastic quantity (per km2 or m3), alongside abundance. Of the two most commonly used methods for calculating plastic concentration (flowmeter and ship's log), ship's log provided consistently smaller abundances, with the exception of one sample, calling for a standardisation in the techniques and measurements used to quantify floating microplastics.