The nitrogen uptake kinetics and physiological growth of Karenia mikimotoi and Skeletonema costatum sensu lato grown on different N substrates and concentrations were compared in the laboratory. In the presence of three N substrates, both species preferred to take up NH₄⁺. K. mikimotoi and S. costatum s.l. showed the highest substrate affinities for urea and NO₃⁻, respectively. Both species grew well on three N substrates, and the growth parameters were comparable among the different N substrates. However, K. mikimotoi assimilated urea more efficiently than it assimilated either NO₃⁻ or NH₄⁺. Different with S. costatum s.l., K. mikimotoi grew slowly and steady and the physiological and growth activities in N-depleted conditions were higher than those in N-replete conditions. Our results suggested that K. mikimotoi shows a greater readiness for uptake and assimilation of urea, and that this species is more competitive in an N-depleted environment when compared with S. costatum s.l.

The aragonite saturation state (Ωₐᵣₐg) was determined to assess its seasonal variations and the major controlling factors in the southeastern Yellow Sea (YS) over four seasons. Ωₐᵣₐg showed large seasonal variation in the surface waters, with dissolved inorganic carbon (DIC) as a major factor controlling the seasonal variation. In the bottom waters, Ωₐᵣₐg exhibited only small seasonal variation compared with the surface waters; DIC and total alkalinity were the main factors contributing to the variation. The bottom water of the southeastern YS was undersaturated with aragonite during the fall, even though the southeastern YS was not typically associated with upwelling, freshwater discharge, or eutrophication processes. Aragonite undersaturation was most likely due to ocean dumping of organic materials. Therefore, ocean pumping should be prohibited in shallow marginal seas to prevent aragonite undersaturation.

Wastewater treatment plants (WWTPs) in coastal region play a primary role in transferring microplastics into the marine environment. Wastewater is closely related to anthropogenic activities, thus the intra-day variation of abundance of microplastics in the influent should be large and could have significant impact on their estimation of the daily mass load. In this study, a 2–hour interval sampling campaign was conducted at a secondary WWTP in Hong Kong to investigate the intra-day variations and daily loads of microplastics in influent. Results show that the average microplastic abundances increased from 7.1 ± 6.0 to 12.8 ± 5.8 particles/L over time, with predominant particle sizes ranging 1–5 mm. Approximately 80% of the microplastics in samples collected from 9:30–15:00 were polyethylene and polyester, while most samples collected at 17:00 were polypropylene and polyurethane. Microplastic loads exhibited large intra-day variations ranging 6.60 × 10⁸–1.16 × 10⁹ particles/day, indicating that calculated daily microplastic loads based on a specific sampling period may inaccurately estimate the actual daily load.

In order to evaluate contamination by polychlorinated biphenyls (PCBs) in a tropical bay exposed to different anthropogenic pressures, samples of bivalves: mangrove oyster (Crassotrea rhizophorae), mangrove mussel (Mytella guyanensis)and clams (Anomalocardia brasiliana), were collected in different parts of Todos os Santos Bay, Bahia, Brazil. In addition, samples of bivalves and fish, purchased from a seafood market in the city of Salvador were analyzed to evaluate human exposure to PCBs through ingestion. Identification and quantification of PCBs were done by GC/MS after microwave extraction and purification with sulfuric acid. In bivalves, concentrations ranged from <0.08 to 50.1 ng g ⁻¹ (dry weight), with the highest values being detected in mangrove oyster, followed by clams and mangrove mussel of the Subaé estuary and Madre de Deus/Mataripe; regions known to be impacted by anthropic activities. From the total of the 12 fish species analyzed, only 5 presented levels of PCBs above the detection limit, ranging from 0.23 to 4.55 ng g ⁻¹ and 0.51 to 26.05 ng g ⁻¹ by dry weight and lipid weight, respectively. In general, concentrations of PCBs on the bay are lower than in most regions around the world, especially those located in the Northern Hemisphere. Indexes indicated that local biota and seafood from the fish market are not adversely impacted by PCBs and do not represent a risk to human health.

Rivers are a major pathway for plastics between lands and the ocean. At the land-ocean interface, estuaries make the transfer dynamic of plastics complex and nonlinear. That is why very little is known about this dynamic. In this respect, a specific marker (i.e. Microlax packaging) showing date-prints was systematically investigated in different riverbanks of the Seine estuary to identify the share of “old” and “recent” litter transiting through the estuary toward the ocean. Up to 70% of Microlax were “old” plastic items probably related to the meandering dynamic of the river over large time and space scales, and hydrodynamic conditions (tides) at smaller scales. This contributes together to increase the residence time of plastics into the estuary up to decades with almost endless transport, deposit and remobilization cycles. Consequently, the Seine estuary may function as a “microplastic factory” resulting from the fragmentation of macroplastics into microplastics well before they reach the ocean.

Red tide has always been an environmental issue with global concern. A Noctiluca scintillans red tide and a Mesodinium red tide occurred successively in Yantai nearshore, China, where is usually oligotrophic, in October 2019. Currents, phytoplankton community composition and nutrients were analyzed to access the driving factors of the red tides. The maximum N. scintillans and Mesodiniium abundance reached 124.92 ± 236.84 × 10³ cells/L and 1157.52 ± 1294.16 × 10³ cells/L respectively. The fast growth of N. scintillans was due to increasing abundance of phytoplankton. The currents were crucial to the assembly and dispersal of red tides. The red tides significantly redistributed the nutrients in the red tide patches and regulated the dominant species in phytoplankton community. Our study illuminates the influence of physical-biochemical coupling processes on red tides, and suggests that ocean dynamics such as currents and tidal factors deserve more attention when considering the ecosystem health problems of coastal zones.

Deposits of fluid mud on wave-dominated beaches are rare. Where they happen frequently and periodically, they can be associated with anthropic processes that bring fluid mud to the shoreface and surf zone. To determine the reason for the frequent deposition of fluid mud at Cassino Beach (Southern Brazil) in the 2014–2020 period, the La/Eu and Gd/Yb ratios in marine, beach, and adjacent Patos estuary sediments were investigated. Mud from the beach showed similar ratios of REE to dredged sediments from the harbor basin and navigation channel within the estuary. Industrial and port activity changes REE ratio in sediments of the navigation channel, and they are distinct from naturally deposited marine and estuarine sediments. We conclude that the mud deposition events at the beach are most likely associated with dredging processes in the estuary especially where overflow is the source of fluid mud on the beach shoreface.

The performance of three approaches for macrobenthic classification in distinguishing communities subjected to different environmental conditions was compared: i) the traditional species-based approach; ii) a tolerance-based approach according to ecological groups of the AMBI index; and iii) a character-based approach using biological traits. We used a hierarchical sampling design including three progressively smaller spatial scales, considering that the environmental degradation influence benthic communities at the larger spatial scale. Despite small-scale spatial variations, all approaches performed similarly, distinguishing eutrophic from non-eutrophic benthic communities as they varied significantly at the larger scale, corresponding to the scale at which human induced changes act. Our results suggest that all three methodological approaches could be used as operational criteria to assess estuarine environmental quality, and despite more meaningful, there is still a degree of subjectivity in functional approaches, associated to the lack of more detailed information on macrofaunal life-cycles and levels of tolerance to disturbance.

Microplastics (MPs) are present in fresh, brackish, or marine waters. Micro- and macroinvertebrates can mistake MPs or small microplastics (SMPs, <100 μm) to be food particles and easily ingest them according to the size of their mouthparts. SMPs may then block the passage of food through the intestinal tract (i.e. hepatopancreas), accumulate within the organism, and enter the food web. Pacific oysters (Crassostrea gigas) are allochthonous filter-feeding bivalve mollusks, which have been introduced in coastal seas around the world in both natural banks and farms. Considering their economic and ecological value, these bivalves have been chosen as a model to study the ingestion of SMPs. A novel method for the extraction and purification of SMPs in bivalves was developed. Quantification and simultaneous polymer identification of SMPs using Micro-FTIR (Fourier Transform Infrared Spectroscopy) were performed, with a limit of detection for the particle size of 5 μm.

We investigated the distribution of microplastics in the water column along a large remote estuarine system located between the Northern and Southern Patagonian Ice Fields in Chilean Patagonia, and connected with the Pacific Ocean through the Gulf of Penas. Microplastic particles were found in all samples, with abundances ranging from 0.1 to 7 particles/m³. Polymers identified were principally acrylics, PET, and cellophane. The average abundance of microplastics in surface waters was similar along the whole estuary (0.4 ± 0.3 particles/m³) with acrylics and epoxy resins being more abundant near Caleta Tortel, the only small village in the area. The observed higher abundance of microplastics in the deeper waters towards the Gulf of Penas points to intrusions of subsurface waters transporting plastic particles from the ocean into the channel system. This underlines the potential of ocean currents in transporting plastic pollution into pristine fjords and channels in Chilean Patagonia.