International audience | Contamination of aquaculture products by pathogenic organisms is a major concern in areas where this activity is of high economic importance. The abundances of total coliforms (TC), Escherichia coli (EC) and faecal strepto-cocci (FS) (in CFU.100 mL-1) in seawater in the Red River coastal aquaculture zone were determined. The results showed TC numbers (200 to 9100; average 1822), EC (<100 to 3400; average 469) and FS (<100 to 2100; average 384), of which TC exceeded the allowable threshold of the Vietnam regulation for coastal aquaculture water. TC and EC numbers in 4 wastewater types (domestic, livestock farming sewage, agricultural runoff, and mixed sewage canals) were investigated and revealed the importance of point sources of faecal contamination in seawater. These results underline the need to reduce the release of untreated wastewater and to put into place seawater microbial quality monitoring in areas where the development of sustainable aquaculture is an objective.

The estuarine ecosystem of Madre de Dios Lagoon (MDL), in the Caribbean Coast of Costa Rica, is exposed to contamination with pesticide residues coming from the upstream agricultural areas. Biomarkers can provide a better indication of the fitness of biota in real mixture exposure scenarios than traditional lethal dose toxicity measurements. Here, we measured biomarkers of biotransformation, oxidative stress, and neurotoxicity on Astyanax aeneus, an abundant fish species in MDL. Glutathione S-transferase activity (GST), catalase activity (CAT), lipid peroxidation (LPO), and cholinesterase activity (ChE) were measured in fish collected during seven sampling campaigns, carried out between 2016 and 2018. Pesticide residues were analyzed in surface water samples collected every time fish were sampled. Residues of 25 pesticides, including fungicides, insecticides, and herbicides, were detected. The biomarkers measured in A. aeneus varied along the sampling moments, with biotransformation and oxidative stress signals showing a coupled response throughout the assessment. Furthermore, significant correlations were established between three biomarkers (GST, LPO, and CAT) and individual pesticides, as well as between GST and LPO with groups of pesticides with shared biocide action. Among pesticides, insecticide residues had a major influence on the responses observed in fish. This work demonstrates the chronic exposure to pesticide residues in MDL and how such exposure is related to physiological responses in fish that can affect their health and potentially, the trophic networks. This early warning information should be considered to improve the protection of estuarine ecosystems in the tropics. | El ecosistema estuarino de la Laguna Madre de Dios (MDL), en la Costa Caribe de Costa Rica, está expuesto a la contaminación con residuos de pesticidas procedentes de las zonas agrícolas situadas aguas arriba. Los biomarcadores pueden proporcionar una mejor indicación de la aptitud de la biota en escenarios reales de exposición a mezclas que las mediciones tradicionales de toxicidad de dosis letales. Aquí medimos biomarcadores de biotransformación, estrés oxidativo y neurotoxicidad en Astyanax aeneus, una especie de pez abundante en MDL. Actividad glutatión S-transferasa (GST), actividad catalasa (CAT), peroxidación lipídica (LPO) y actividad colinesterasa (ChE) se midieron en peces recolectados durante siete campañas de muestreo, realizadas entre 2016 y 2018. Residuos de pesticidas se analizaron en muestras de agua superficial recolectadas cada vez que se tomaron muestras de peces. Residuos de 25 pesticidas, incluidos fungicidas, Se detectaron insecticidas y herbicidas. Los biomarcadores medidos en A. aeneus variaron a lo largo de los momentos de muestreo, con señales de biotransformación y estrés oxidativo que muestran una respuesta acoplada a lo largo de la evaluación. Además, Se establecieron correlaciones significativas entre tres biomarcadores (GST, LPO y CAT) y pesticidas individuales, así como como entre GST y LPO con grupos de plaguicidas con acción biocida compartida. Entre los plaguicidas, los residuos de insecticidas habían una influencia importante en las respuestas observadas en los peces. Este trabajo demuestra la exposición crónica a residuos de pesticidas en MDL y cómo dicha exposición se relaciona con respuestas fisiológicas en los peces que pueden afectar su salud y, potencialmente, la redes tróficas. Esta información de alerta temprana debe considerarse para mejorar la protección de los ecosistemas estuarinos. en los trópicos. | Universidad Nacional, Costa Rica | Springer, Alemania | Escuela de Ciencias Biológicas

The global increase of plastic production, linked with an overall plastic misuse and waste mismanagement, leads to an inevitable increase of plastic debris that ends up in our oceans. One of the major sinks of this pollution is the deep-sea floor, which is hypothesized to accumulate in its deepest points, the hadal trenches. Little is known about the magnitude of pollution in these trenches, given the remoteness of these environments, numerous factors influencing the input and sinking behavior of plastic debris from shallower environments. This study represents to the best of our knowledge the largest survey of (macro)plastic debris sampled at hadal depths, down to 9600 m. Industrial packaging and material assignable to fishing activities were the most common debris items in the Kuril Kamchatka trench, most likely deriving from long-distance transport by the Kuroshio extension current (KE) or from regional marine traffic and fishing activities. The chemical analysis by (Attenuated Total Reflection Fourier transform infrared (ATR-FTIR) spectroscopy revealed that the main polymers detected were polyethylene (PE), polypropylene (PP) and nylon. Plastic waste is reaching the depths of the trench, although some of the items were only partially broken down. This finding suggests that complete breakdown into secondary microplastics (MP) may not always occur at the sea surface or though the water column. Due to increased brittleness, plastic debris may break apart upon reaching the hadal trench floor where plastic degrading factors were thought to be, coming off. The KKT's remote location and high sedimentation rates make it a potential site for high levels of plastic pollution, potentially making it one of the world's most heavily contaminated marine areas and an oceanic plastic deposition zone.

peer reviewed | Contamination of land and aquatic ecosystems with heavy metals (HMs) is a global issue having the persistent potential to damage the quality of food and water. In the present study, Tagetes erecta L. plants were used to assess their potential to uptake HMs from wastewater. Plants were grown in soil for 20 days and then transplanted in hydroponic system containing Hoagland nutrient solution. After more than 15 days of growth, plants were then subjected to wastewater from tannery and surgical industries in different concentrations ranging from 25 to 100% in combination of citric acid (5 and 10 mM). After 6 weeks of treatment, plants were collected and segmented into roots, stem, and leaves for characterizing the morphological properties including plant height, roots length, fresh and dry mass of roots, stem, and leaves. For evaluation of the effect of wastewater on the plants, photosynthetic pigments; soluble proteins; reactive oxygen species (ROS); antioxidant enzymes SOD, POD, CAT, and APX; and metal accumulation were analyzed. Application of industrial wastewater revealed a significant effect on plant morphology under wastewater treatments. Overall growth and physiological attributes of plant decreased, and metal accumulation enhanced with increasing concentration of wastewater. Similarly, the production of ROS and antioxidant enzymes were also increased. Chlorophyll, protein content, and enzyme production enhanced with CA (5 and 10 mM) mediation; however, ROS production and EL were reduced. Metals analysis showed that the maximum accumulation of Pb was in roots, while Cr and Ni in the stem which further increased under CA mediation. Overall, the metal accumulation ability was in the order of Pb > Ni > Cr under CA.

International audience | Human urine concentrates 88% of the nitrogen and 50% of the phosphorus excreted by humans, making it a potential alternativecrop fertilizer. However, knowledge gaps remain on the fate of nitrogen in situations favouring NH3 volatilization and on theavailability of P from urine in soils. This study aimed at identifying the fate of nitrogen and phosphorus supplied by human urinefrom source separation toilets in a calcareous soil. To this end, a spinach crop was fertilized with 2 different doses of human urine and compared with a synthetic fertilizer treatment and an unfertilized control. The experiment was conducted in 4 soil tanks (50-cm depth) in greenhouse condi-tions, according to a randomized block scheme. We monitored soil mineral nitrogen over time and simulated nitrogen volatiliza-tion using Hydrus-1D and Visual Minteq softwares. We also monitored soil phosphorus pools, carbon, nitrogen and phosphorus(CNP) in microbial biomass, soil pH and electrical conductivity. Only an excessive input of urine affected soil pH (decreasing itby 0.2 units) and soil conductivity (increasing it by 183%). The phosphorus supplied was either taken up by the crop or remainedmostly in the available P pool, as demonstrated by a net increase of the resin and bicarbonate extractable P. Ammonium seemedto be nitrified within about 10 days after application. However, both Visual Minteq and Hydrus models estimated that more than50% of the nitrogen supplied was lost by ammonia volatilization. Overall, our results indicate that direct application of urine to acalcareous soil provides available nutrients for plant growth, but that heavy losses of volatilized nitrogen are to be expected. Ourresults also question whether long-term application could affect soil pH and salinity.

International audience | Neonicotinoids are the most widely used class of insecticides in the world but they have raised numerous concerns regarding their effects on biodiversity. Thus, the objective of this work was to do a critical review of the contamination of the environment (soil, water, air, biota) by neonicotinoids (acetamiprid, clothianidin, imidacloprid, thiacloprid, thiamethoxam) and of their impacts on terrestrial and aquatic biodiversity. Neonicotinoids are very frequently detected in soils and in freshwater, and they are also found in the air. They have only been recently monitored in coastal and marine environments, but some studies already reported the presence of imidacloprid and thiamethoxam in transitional or semi-enclosed ecosystems (lagoons, bays and estuaries). The contamination of the environment leads to the exposure and to the contamination of non-target organisms, and to negative effects on biodiversity. Direct impacts of neonicotinoids are mainly reported on terrestrial invertebrates (e.g., pollinators, natural enemies, earthworms) and vertebrates (e.g., birds), and on aquatic invertebrates (e.g., arthropods). Impacts on aquatic vertebrate populations and communities, as well as on microorganisms, are less documented. In addition to their toxicity to directly exposed organisms, neonicotinoid induce indirect effects via trophic cascades as demonstrated in several species (terrestrial and aquatic invertebrates). However, more data are needed to reach firmer conclusions and to get a clearer picture of such indirect effects. Finally, we identified specific knowledge gaps that need to be filled to better understand the effects of neonicotinoids on terrestrial, freshwater and marine organisms, as well as on ecosystem services associated with these biotas.

International audience | The adverse outcome pathway (AOP) has been conceptualized in 2010 as an analytical construct to describe a sequential chain of causal links between key events, from a molecular initiating event leading to an adverse outcome (AO), considering several levels of biological organization. An AOP aims to identify and organize available knowledge about toxic effects of chemicals and drugs, either in ecotoxicology or toxicology, and it can be helpful in both basic and applied research and serve as a decision-making tool in support of regulatory risk assessment. The AOP concept has evolved since its introduction, and recent research in toxicology, based on integrative systems biology and artificial intelligence, gave it a new dimension. This innovative in silico strategy can help to decipher mechanisms of action and AOP and offers new perspectives in AOP development. However, to date, this strategy has not yet been applied to ecotoxicology. In this context, the main objective of this short article is to discuss the relevance and feasibility of transferring this strategy to ecotoxicology. One of the challenges to be discussed is the level of organisation that is relevant to address for the AO (population/community). This strategy also offers many advantages that could be fruitful in ecotoxicology and overcome the lack of time, such as the rapid identification of data available at a time t, or the identification of “data gaps”. Finally, this article proposes a step forward with suggested priority topics in ecotoxicology that could benefit from this strategy.

International audience | Triazoles belong to a family of fungicides that are ubiquitous in agroecosystems due to their widespread use in crops. Despite their efficiency in controlling fungal diseases, triazoles are also suspected to affect non-target vertebrate species through the disruption of key physiological mechanisms. Most studies so far have focused on aquatic animal models, and the potential impact of triazoles on terrestrial vertebrates has been overlooked despite their relevance as sentinel species of contaminated agroecosystems. Here, we examined the impact of tebuconazole on the thyroid endocrine axis, associated phenotypic traits (plumage quality and body condition) and sperm quality in wild-caught house sparrows (Passer domesticus). We experimentally exposed house sparrows to realistic concentrations of tebuconazole under controlled conditions and tested the impact of this exposure on the levels of thyroid hormones (T3 and T4), feather quality (size and density), body condition and sperm morphology. We found that exposure to tebuconazole caused a significant decrease in T4 levels, suggesting that this azole affects the thyroid endocrine axis, although T3 levels did not differ between control and exposed sparrows. Importantly, we also found that exposed females had an altered plumage structure (larger but less dense feathers) relative to control females. The impact of tebuconazole on body condition was dependent on the duration of exposure and the sex of individuals. Finally, we did not show any effect of exposure to tebuconazole on sperm morphology. Our study demonstrates for the first time that exposure to tebuconazole can alter the thyroid axis of wild birds, impact their plumage quality and potentially affect their body condition. Further endocrine and transcriptomic studies are now needed not only to understand the underlying mechanistic effects of tebuconazole on these variables, but also to further investigate their ultimate consequences on performance (i.e. reproduction and survival).

International audience | Rivers are representative of the overall contamination found in their catchment area. Contaminant concentrations in watercourses depend on numerous factors including land use and rainfall events. Globally, in Mediterranean regions, rainstorms are at the origin of fluvial multipollution phenomena as a result of Combined Sewer Overflows (CSOs) and floods. Large loads of urban-associated microorganisms, including faecal bacteria, are released from CSOs which place public health - as well as ecosystems - at risk. The impacts of freshwater contamination on river ecosystems have not yet been adequately addressed, as is the case for the release of pollutant mixtures linked to extreme weather events. In this context, microbial communities provide critical ecosystem services as they are the only biological compartment capable of degrading or transforming pollutants. Through the use of 16S rRNA gene metabarcoding of environmental DNA at different seasons and during a flood event in a typical Mediterranean coastal river, we show that the impacts of multipollution phenomena on structural shifts in the particle-attached riverine bacteriome were greater than those of seasonality. Key players were identified via multivariate statistical modelling combined with network module eigengene analysis. These included species highly resistant to pollutants as well as pathogens. Their rapid response to contaminant mixtures makes them ideal candidates as potential early biosignatures of multipollution stress. Multiple resistance gene transfer is likely enhanced with drastic consequences for the environment and human-health, particularly in a scenario of intensification of extreme hydrological events.

International audience | Abstract Botanicals are various plant-based products like plant extracts or essential oils. Anti-fungal activities of selected essential oils were tested on the pathogen causing potato and tomato late blight ( Phytophthora infestans ). Tests to evaluate anti-oomycete activities of commercial essential oils and their major compounds were carried out in vitro in microplate in liquid media. Anti-oomycete activities on Phytophthora infestans strain were obtained from essential oils/major compounds: Eucalyptus citriodora /citronellal; Syzygium aromaticum (clove)/eugenol; Mentha spicata /D-Carvone, L-Carvone; Origanum compactum /carvacrol; Satureja montana (savory)/carvacrol; Melaleuca alternifolia (tea tree)/terpinen-4-ol, and Thymus vulgaris /thymol. As an active substance of mineral origin, copper sulfate was chosen as a control. All selected essential oils showed an anti-oomycete activity calculated with IC 50 indicator. The essential oils of clove, savory, and thyme showed the best anti-oomycete activities similar to copper sulfate, while oregano, eucalyptus, mint, and tea tree essential oils exhibited significantly weaker activities than copper sulfate. Clove essential oil showed the best activity (IC 50 = 28 mg/L), while tea tree essential oil showed the worst activity (IC 50 = 476 mg/L). For major compounds, three results were obtained: they were statistically more active than their essential oils (carvacrol for oregano, D- and L-Carvone for mint) or as active as their essential oils sources (thymol for thyme, carvacrol for savory, terpinen-4-ol for tea tree) or less active than their original essential oils (eugenol for clove, citronellal for eucalyptus). Microscopical observations carried out with the seven essential oils showed that they were all responsible for a modification of the morphology of the mycelium. The results demonstrated that various essential oils show different anti-oomycete activities, sometimes related to a major compound and sometimes unrelated, indicating that other compounds must play a role in total anti-oomycete activity.