[Departement_IRSTEA]Eaux [TR1_IRSTEA]ARCEAU [TR2_IRSTEA]TED | International audience | In the context of pesticide pollution in hydrosystem, limiting pesticide transfer from agricultural plot to surface waterbodies appears to be crucial. Two constructed wetlands were tested at both pilot scale of an experimental constructed wetland and field scale of the outfall of a subsurface drained watershed. Tracer experiment and pesticide mass balance studies allowed us to assess the dissipation potential of a subsurface flow constructed wetland. At field scale, climatic parameters water and pesticide's flows in and out have been measured and monitored. We also recorded the conditions of implementation since we were very closed to real conditions. For this purpose inquiries addressing the various actors were carried out by sociologists. The results of the performance regarding pesticide's dissipation are given mentioning that efficiency is strongly linked to pesticide properties and hydrological transfer period (from 20 to 90% of pesticide removal). Sociologic approaches and amenities assessments have revealed unsuspected relations of the farmers with the society and the environment, and vice versa. The implementations have resulted of a co-construction where each actor had personal involvement. Even if co-construction should be a driving line, solutions for appropriate incentives and land reallocation tools should be fought with politics and authorities in order to facilitate further realisations.

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International audience

[Departement_IRSTEA]Ecotechnologies [TR1_IRSTEA]TED [Axe_IRSTEA]TED-EPURE | International audience | Thus, choosing to plant local macrophytes species in CWs can be considered as an asset for the development of CWs, since it can be a part of global development projects which integrate cultural, social and economic characteristics. But, because of the ability of some macrophytes to accumulate toxic elements, human utilisation of CWs macrophytes has to be managed considering health damage potentiality. Spontaneous macrophytes diversity has to be appreciated for its key-role in the development of CWs in developing countries, not only from an ecological point of view, but also from cultural, social, sanitary and economic views.

[Departement_IRSTEA]Ecotechnologies [TR1_IRSTEA]TED [Axe_IRSTEA]TED-EPURE | International audience | This paper presents the key elements that emerged from a 3-year study on 4 campsites. Each of them has its own Vertical-Flow Constructed Wetlands (VFCW) for on-site wastewater treatment. Each year, the pollution discharge peaked in the 5 to 6-week summer period but was zero for a 6-month period. The natural-concentrate wastewater is collected into a short sewer network. Hydraulic load and campground occupancy rate were tightly correlated. The effluent has a characteristically high nitrogen concentration due to the lower greywater-producing activity. These quantitative and qualitative elements were used to define the camper equivalent' as a daily pollutant load of about 100 L water, 90 gCOD, 35 gBOD5, 40 gSS, 11 gKN and 1.4 gPt. During the 5-6 weeks of peak visitor traffic, the full first stage receives an organic load of 200 gCOD.m-2.d-1 and 25 gKN.m-2.d-1, which is 2 to 3.5-fold higher than the loads conventionally applied in VFCW systems in France. Despite these heavy loads, COD removal remains constant (at 91%) over the season. During the summer peak, nitrification drops sharply but KN removal rate holds steady near 60%. This study demonstrates that VFCWs can robustly handle massive load increases. The paper concludes that total system size can be downscaled to 0.75 m²/camper. This design allows to reach minimum yields of 75% KN, provided that the system is built and run to state-of-the-art rules. | Cet article présente les éléments clés issus d'une étude de 3 ans sur 4 campings qui traitent leurs eaux usées de manière individuelle par des Filtres Plantés de Roseaux à écoulement vertical (FPRV) aux dimensionnements différents. La charge hydraulique et le taux d'occupation du camping sont étroitement corrélés. L'émission est nulle 6 mois par an et la pointe estivale dure 5 à 6 semaines. Les eaux usées, collectées par un réseau court sont naturellement concentrées. L'effluent présente notamment une très forte concentration en azote du fait d'une activité ménagère réduite. Ces éléments qualitatifs et quantitatifs permettent de définir l' « équivalent campeur » par une charge de pollution journalière d'environ 100 L d'eau, 90 g de DCO, 35 g de DBO5, 40 g de MES, 11 g de NK et 1,4 g de Pt. Au cours des 5-6 semaines de fréquentation maximale, la totalité du 1er étage reçoit une charge organique d'environ 200 gDCO.m-².j-1 et 25 gNK.m-².j-1, soit 2 à 3,5 fois plus que les charges classiques appliquées en FPRV en France. En dépit de ces fortes charges, l'élimination de la DCO reste constante (91 %) au cours de la saison. Lors du pic de fréquentation estival, la nitrification décroît fortement mais le rendement global se maintient à environ 60 %. La robustesse des FPRV face à une augmentation massive des charges appliquées est démontrée. L'article conclut par un dimensionnement réduit de 0.75 m2/campeur au total. Ce dimensionnement permet d'atteindre un rendement minimum de 75 % du NK, correspondant à la nitrification, sous réserve que les règles de l'art de construction et d'exploitation soient strictement respectées.

The aim of this work was to investigate if engineered endophytes can improve phytoremediation of co-contaminations by organic pollutants and toxic metals. As a model system, yellow lupine was inoculated with the endophyte Burkholderia cepacia VM1468 possessing (a) the pTOM-Bu61 plasmid, coding for constitutive trichloroethylene (TCE) degradation, and (b) the ncc-nre Ni resistance/sequestration system. Plants were exposed to Ni and TCE and (a) Ni and TCE phytotoxicity, (b) TCE degradation and evapotranspiration, and (c) Ni concentrations in the roots and shoots were determined. Inoculation with B. cepacia VM1468 resulted in decreased Ni and TCE phytotoxicity, as measured by 30% increased root biomass and up to 50% decreased activities of enzymes involved in anti-oxidative defence in the roots. In addition, TCE evapotranspiration showed a decreasing trend and a 5 times higher Ni uptake was observed after inoculation.

Accumulation of free microcystins (MCs) in freshwater gastropods has been demonstrated but accumulation of MCs covalently bound to tissues has never been considered so far. Here, we follow the accumulation of total (free and bound) MCs in Lymnaea stagnalis exposed to i) dissolved MC-LR (33 and 100 μg L−1) and ii) Planktothrix agardhii suspensions producing 5 and 33 μg MC-LR equivalents L−1 over a 5-week period, and after a 3-week depuration period. Snails exposed to dissolved MC-LR accumulated up to 0.26 μg total MCs g−1 dry weight (DW), with no detection of bound MCs. Snails exposed to MCs producing P. agardhii accumulated up to 69.9 μg total MCs g−1 DW, of which from 17.7 to 66.7% were bound. After depuration, up to 15.3 μg g−1 DW of bound MCs were detected in snails previously exposed to toxic cyanobacteria, representing a potential source of MCs transfer through the food web. The study concerns accumulation and elimination of both free and bound microcystins (MCs) in tissues of a gastropod exposed to MCs producing cyanobacteria or dissolved MC-LR.

This study examines the effects of erythromycin on activated sludge from two French urban wastewater treatment plants (WWTPs). Wastewater spiked with 10 mg/L erythromycin inhibited the specific evolution rate of chemical oxygen demand (COD) by 79% (standard deviation 34%) and the specific N–NH4+ evolution rate by 41% (standard deviation 25%). A temporary increase in COD and tryptophan-like fluorescence, as well as a decrease in suspended solids, were observed in reactors with wastewater containing erythromycin. The destruction of activated sludge flocs was monitored by automated image analysis. The effect of erythromycin on nitrification was variable depending on the sludge origin. Erythromycin inhibited the specific nitrification rate in sludge from one WWTP, but increased the nitrification rate at the other facility. Erythromycin toxicity on activated sludge is expected to reduce pollution removal.

There is strong interest in developing tools to link chemical concentrations of contaminants to the potential for observing sediment toxicity that can be used in initial screening-level sediment quality assessments. This paper presents new approaches for predicting toxicity in sediments, based on 10-day survival tests with marine amphipods, from sediment chemistry, by means of the application of Partial Least Squares-Discriminant Analysis (PLS-DA) and Counter-propagation Artificial Neural Networks (CP-ANNs) to large historical databases of chemical and toxicity data. The exploration of the internal structure of the developed models revealed inherent limitations of predicting toxicity from common chemical analyses of bulk contaminant concentrations. However, the results obtained in the validation of these models combined relevant values of non-error classification rate, sensitivity and specificity of, respectively, 76, 87 and 73% with PLS-DA and 92, 75 and 97% with CP-ANNs, outperforming the results reported for previous approaches. Models for predicting toxicity based on amphipod tests, derived using PLS-DA and CP-ANN, can be useful aids for screening-level sediment quality assessment.