The introduction and spread of marine non-indigenous species (NIS) and pathogens into new habitats are a major threat to biodiversity, ecosystem services, human health, and can have substantial economic consequences. Shipping is considered the main vector for marine biological invasions; less well understood is the increased spread of marine NIS and pathogens rafting on marine plastic debris (MPD). Despite an increasing research interest and recent progress in characterizing the plastisphere, this manuscript highlights critical knowledge gaps and research priorities towards a better understanding of the biosecurity implications of MPD. We advocate for future research to (i) investigate plastisphere community succession and the factors influencing NIS propagules and pathogens recruitment through robust experimental investigations; (ii) combine microscopy and molecular approaches to effectively assess the presence of specific taxa; (iii) include additional genetic markers to thoroughly characterize the biodiversity associated with MPD and explore the presence of specific marine pests.

This study focuses on assessing litter magnitudes along the Torre Guaceto Marine Protected Area (Brindisi, Italy). Collected litter was grouped into twenty different types and classified into four litter typologies according to the Guidance on Monitoring of Marine Litter in European Seas. All data were analyzed using an index-based approach that allowed the classification of a coastal stretch in terms of cleanliness, and presence of plastics as well hazardous items. The average litter abundance in the study area was 0.5 items/m², being plastics the most common litter item. Hazardous litter items were found along the study area, reaching 21.3% of the total collected items. The application of environmental indices allowed to define the study area with a “moderate cleanliness” and a “moderate” presence of hazardous litter items. Sampled litter typologies and related magnitudes suggest a combination of sources that mainly include direct activities on the nearby coastal zones and river basins (dumping).

The presence of pharmaceuticals in surface water and sediment has sparked up a global concern, as they could cause harm to human health. In this study, we investigated five pharmaceuticals (caffeine, carbamazepine, sulfamethoxazole, testosterone, and trimethoprim) in surface water and sediment samples from Swartkops River Estuary and Markman Stormwater Canal, in the Eastern Cape Province, South Africa. Ultra-Performance Liquid Chromatography (UPLC) systems coupled with a hyphenated quadrupole-time-of-flight mass spectrometry (QTOF-MS) was used for the analysis. Of the five pharmaceuticals investigated, three were detected in sediment samples at concentrations ranging from BDL – 23.86 μg/kg (dw). Caffeine and sulfamethoxazole were below the detection limit. The finding of this current study suggests that Markman and Motherwell's stormwater canals were potential contributors to pollution in Swartkops River Estuary. Ecotoxicity risk assessment indicated that trimethoprim and carbamazepine could constitute potential risk to aquatic organisms in Markman Canal and Swartkops Estuary, suggesting the need for proper control measure to prevent the pollution from toxicants in aquatic resources.

Measuring environmental contaminants in coastal areas is critical for monitoring and managing their impacts. Commonly used techniques involve repetitive field sampling, which provides a single moment in time during each effort. In this study, we examine the potential for using foraminifera in monitoring and risk assessment as recorders of bioavailable pollutants. Geochemical analysis of benthic foraminifera (Operculina ammonoides) shells sampled annually at a previous fish farm location showed extremely high levels of copper (Cu), zinc (Zn), and phosphorus (P) during the three years following the fish cages removal, with a general reduction afterwards. Levels of Cu/Ca were still more than 4-fold higher than background levels 10 years after the removal of the fish cages. Based on our finds, it is concluded that the geochemical analysis of recent benthic foraminifera shells can serve as a powerful monitoring tool of bioavailable contaminants in seawater. Additionally, the results highlight the need for heavy metal monitoring near marine aquaculture facilities and suggest that long-term effects extend spatially and temporally far beyond the original point source. Finally, we observed variations in micro-distribution of elements within the top 1–2 μm of the shells, where Cu/Ca, Zn/Ca and P/Ca are consistently higher in the chamber wall than in the septa. This observation is relevant for studies conducting single chamber analyses.

Phytoplankton and accompanying environmental data (temperature, salinity, secchi depth, stratification, and inorganic nutrients) were analyzed from 672 surface water samples (0 to 1.5 m depth) collected from 95 stations located on the Louisiana shelf between April 1990 and August 2011. Phytoplankton were identified to the lowest practical taxonomic unit from glutaraldehyde-preserved samples using epifluorescent microscopy and reported as cells L⁻¹. Twenty-six phytoplankton taxa (primarily diatoms) that were > 8 μm in size, identified to genus-level resolution and ranked in the top 20 in at least one of three separate categories (average abundance; frequency of occurrence; and bloom frequency) were used in subsequent analyses. Temperature, stratification, and secchi depth constituted the environmental variable combination best related to the phytoplankton community composition patterns across the 672 samples (r = 0.288; p < 0.01) according to BEST analysis (PRIMER 7). The environmental optima of the 26 taxa were calculated using the weighted-averaging algorithm in the C2 program and then used to group the taxa into common phytoplankton clusters (i.e., niches) using PRIMER 7 CLUSTER. The phytoplankton clustered into three groups: Group A (summer assemblage), Group B (winter assemblage), and Group C (spring bloom assemblage). The results demonstrate that the composition of the phytoplankton community is most related to seasonality and physical variables, whereas nutrients appear to play a larger role in driving overall phytoplankton biomass. This study provides a platform to examine phytoplankton responses to future environmental perturbations in the region.

In this study, selected PCB congeners (IUPAC numbers 28, 52, 101, 138, 153, and 180) were quantified in 34 stations of Chabahr bay and around it in the Makran region of Iran. The sum of total PCB concentrations varied from below the detection limit to 485 ng kg⁻¹ dry weight of sediment. Based on the Canadian Sediment Quality Guidelines, the effect of detected PCBs was negligible for aquatic organisms. According to the dominance of PCB 28 and 52 with average range of 62 to 100% of total PCBs, maritime transportation and atmospheric deposition appear to be the important source of PCBs in this region. Further, the presence of components of commercial products such as ClophenA50 appears to be one of the probable sources.

Humans have always benefited from marine ecosystems and the use of their services has increased over time. The principal challenge of managing ecosystem services is that they are not independent of each other. Attempts to optimize a single service have often led to the reductions or losses of other services; in other words, they are “traded-offs”. Based on the purpose of assessing the impacts of marine aquaculture development in the Southern Caspian Sea, 3 management scenarios were utilized to model 3 categories, including marine aquaculture, Water Quality (WQ) and Habitat Risk Assessment (HRA) through marine Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST). Marine aquaculture model was first considered for Oncorhynchus mykiss that the results were as follows: in the baseline scenario with 9 farms, the amounts of the total weight of fish produced and Net Present Value (NPV) measured during 1 year equaled 1970 tons and 2,247,000 dollars; in the conservation scenario with 4 farms, their amounts were 800 tons and 1 million dollars; in the expansion scenario with an increased number of farms (20 farms), their amounts were estimated to be 4000 tons and 5 million dollars, respectively. The results of the marine aquaculture model were utilized as the inputs both to WQ (amount of BOD released from each farm) and HRA (habitats of four species of fish (Rutilus frisii kutum, Acipenser stellatus, Acipenser persicus, and Huso huso) models. The results revealed 41%, 16%, and 60% of WQ reductions and 35%, 20%, and 45% of HRA changes in the baseline, conservation, and expansion scenarios, respectively. Considering the current production of 9 existing farms (1970 tons per year) and the impact of this production on water quality (41%) and habitat risk (35%), it is recommended to reduce this production volume and doing aquaculture development in the Southern Caspian Sea without considering and managing negative consequences can have dangerous consequences for this sensitive ecosystem.

To avoid loss of genetic information in environmental DNA (eDNA) field samples, the preservation of nucleic acids during field sampling is a critical step. In the development of standard operating procedures (SOPs) for eDNA-based compliance monitoring, the effect of different routinely used sediment preservations on biological community structures serving as bioindicators has gone untested. We compared eDNA metabarcoding results of marine bacterial communities from sample aliquots that were treated with a nucleic acid preservation solution (treated samples) and aliquots that were frozen without further treatment (non-treated samples). Sediment samples were obtained from coastal locations subjected to different stressors (aquaculture, urbanization, industry). DNA extraction efficiency, bacterial community profiles, and measures of alpha- and beta-diversity were highly congruent between treated and non-treated samples. As both preservation methods provide the same relevant information to environmental managers and regulators, we recommend the inclusion of both methods into SOPs for biomonitoring in marine coastal environments.

Many tropical river systems have altered water quality due to human land use, impacting the biodiversity of freshwater and coastal ecosystems. Long-term, catchment-scale monitoring is needed to understand pollutant sources, controls, and trends. This 12-year study monitored baseflow and flood event nutrient and sediment concentrations, and estimated sediment loads across the Normanby Basin in northern Australia. Suspended sediment concentrations and yields were highest in upper catchment areas where cattle grazing occurred on erosion-prone sodic soils. Mid- and lower catchment rivers and floodplains were a sink for sediments and nutrients, trapping around 75% of suspended sediments during events. Clays (<4 μm) were preferentially transported to the estuary, with an estimated 46% sediment delivery ratio. In the estuary, suspended sediment concentrations were influenced by tidal resuspension processes and there were significant sources of DIN. These findings can help prioritise land management investments for the protection of Great Barrier Reef and freshwater ecosystems.