This article summarizes historical and recent research on the terrestrial toxicology of tetrabromobisphenol A (TBBPA). Despite its ubiquitous use and presence in the environment, little published data is available to evaluate the terrestrial ecotoxicity of TBBPA. The purposes of this paper are to enable broad access to a series of TBBPA ecotoxicity tests (nitrogen transformation, earthworm survival/reproduction, and seedling emergence/growth) that were conducted in support of regulatory risk assessments, and to summarize available research in the terrestrial toxicity of TBBPA. In these studies, no significant effect of TBBPA on nitrogen transformation was observed up to the highest concentration [1000 mg/kg dry weight (d.w.) soil]. The no-observed-effect concentrations (NOECs) for seedling emergence ranged from 20 to 5000 mg/kg d.w. Sensitivities were soybeans < corn ≈ onion ≈ tomato < ryegrass < cucumber; the most sensitive endpoints being seedling dry weight and height. The 28-day earthworm mortality NOEC was > 4840 mg/kg d.w. The most sensitive terrestrial endpoint was earthworm reproduction with a half maximal effective concentration (EC₅₀) of 0.12 mg/kg d.w. soil. Based on this sensitive terrestrial endpoint, the EU derived a predicted no-effect concentration (PNEC) for soil of 0.012 mg/kg wet weight soil (EU 2008). We did not identify a more sensitive/lower point of departure for terrestrial toxicity endpoints in the published literature. On the basis of this PNEC, the EU concluded there was potential risk for environmental effects near TBBPA manufacturing sites, but no additional risk provided that no sewage sludge was applied to agricultural land (EU 2008).

Toxic effects of continuous low dose application of the antifouling biocide chlorine on marine benthic organisms were monitored using transplanted green mussels (Perna viridis) and a suite of biomarkers. Caged mussels were deployed in chlorinated and non-chlorinated sections of the cooling system of an operating electric power plant. Biomarkers indicative of general stress, oxidative stress (superoxide dismutase and catalase), and DNA integrity, along with expression of stress proteins, were studied to assess the effects. Deterioration in condition index with corresponding increase in DNA strand breaks was indicative of chlorine stress. Superoxide dismutase enzyme did not show any particular trend, but catalase activity was high during the initial days of exposure at the chlorinated site; later, it became almost equal to that at the control site. Similarly, expressions of stress proteins (HSP60, HSP70, HSP22, GSTS1, and CYP4) showed bell-shaped pattern during the period of study. Positive correlation among the endpoints indicated the utility of the multimarker approach to monitor the effects of continuous low dose chlorination on mussels.

The photochemical removal of benzene was studied in air at atmospheric pressure using a side-on type 172 nm Xe₂ excimer lamp with a wide irradiation area. After 1.5 min photoirradiation, C₆H₆ (1000 ppm) in air was completely converted to HCOOH, CO, and CO₂ at a total flow rate of 1000 mL/min. The initial decomposition rate of C₆H₆ was determined to be 1.18 min⁻¹. By using a flow system, C₆H₆ (200 ppm) was completely removed at a total flow rate of 250 mL/min. The conversion of C₆H₆ and the energy efficiency in the removal of C₆H₆ changed in the 31−100% and 0.48−1.2 g/kWh range, respectively, depending on the flow rate, the O₂ concentration, and the chamber volume. On the basis of kinetic model simulation, dominant reaction pathways were discussed. Results show that the O(³P) + C₆H₆ reaction plays a significant role in the initial stage of the C₆H₆ decomposition. Important experimental parameters required for further improvement of the C₆H₆ removal apparatus using a 172 excimer lamp were discussed based on model calculations.

Simulation experiments were conducted using sediments collected from the Taihu Lake to determine the combined effects of submerged macrophytes Ceratophyllum demersum and phosphate-solubilizing bacteria (PSB) strain XMT-5 (Rhizobium sp.) on phosphorus (P) concentrations in overlying waters and sediments. After 30 days of experimental incubation, the total phosphorus (TP) and dissolved total phosphorus (DTP) concentrations of the overlying water subjected to AMB and AHMB treatments (both with the combined effects of PSB cells and submerged macrophytes) were generally lower than those of the AM (with individual effects of inoculated C. demersum) and AB (with individual effects of a smaller amount of inoculated PSB cells) control treatments but higher than that of the A (with no effects of inoculated PSB cells or C. demersum) and AHB (with individual effects of a larger amount of inoculated PSB) control treatments. The TP contents of the sediment in the AMB and AHMB treatments were significantly lower than those of the other control treatments. The TP contents of the C. demersum cocultured with the PSB strain XMT-5 cells in the AMB and AHMB treatments were all significantly higher than that of the AM treatment, indicating the enhancement of P uptake by submerged plants inoculated with PSB. The bacterial diversity structures of the rhizosphere sediment subjected to different treatments were also analyzed by the high-throughput sequencing method. According to the ACE and Chao 1 indices, the bacterial diversity in the AMB and AHMB treatments were the highest. Although many sources contributed to the decrease in the nutrient loads of the lake sediment, harvesting macrophytes inoculated with PSB cells prior to their senescence might constitute a significant in-lake measure for reducing internal P load.

Atmospheric particulate matter (APM), commonly seen and widely excited in environment, appears great enough to influence the biochemical processes in aquatic microorganisms and phytoplankton. Understanding the response of cyanobacteria to various factors is fundamental for eutrophication control. To clarify the response of cyanobacteria to APM, the effects of PM₂.₅, PM₂.₅–₁₀, and PM>₁₀ on Microcystis aeruginosa were researched. Variabilities in cell density, chlorophyll a, soluble protein, malondialdehyde, extracellular activity, and kinetic parameters of alkaline phosphatase were evaluated by lab-cultured experiments. Results showed that the PM₂.₅ had a slight stimulation impact on the growth and enhanced both of the 48- and 72-h extracellular alkaline phosphatase activity (APA), the affinity of alkaline phosphatase for substrate, and the 72-h maximum enzymatic reaction velocity (Vₘₐₓ). Moreover, the stimulations in extracellular APA and Vₘₐₓ enhanced with the increasing exposure concentrations. We also found there were no obvious distinctions on the effects of growth and alkaline phosphatase in M. aeruginosa between PM₂.₅–₁₀ and PM>₁₀ exposure groups. Obviously, inhibitory effects on growth existed in 4.0 and 8.0 mg/L PM₂.₅–₁₀ and 8.0 mg/L PM>₁₀ at 120 h. Furthermore, PM₂.₅–₁₀ and PM>₁₀ exerted inhibitory effects on the extracellular APA during the 72-h exposure. Simultaneously, the Vₘₐₓ was notably inhibited and the affinity of alkaline phosphatase for substrate was more inseparable compared with control in PM₂.₅–₁₀ and PM>₁₀ treatments. Nevertheless, the inhibitors in extracellular APA and kinetic parameters were unrelated to PM₂.₅–₁₀ and PM>₁₀ exposure concentrations. Two-way ANOVA results revealed that there were significant interactions between exposure concentration and diameter of APM on the 120-h cell density, soluble protein content, APA, and 72 h APA of M. aeruginosa. These results in our study would be meaningful to further researches on relationships between APM deposition and cyanobacterial bloom.

The aim of the present study was to investigate three kinds of emergent macrophytes, i.e., Acorus calamus L., Phragmites communis Trin., and Alternanthera philoxeroides (Mart.) Griseb and their combination patterns on their removal efficiency of compound heavy metals (vanadium, chromium, and cadmium) from synthetic aqueous. The results showed that the optimal single-species for compound heavy metals removal was Acorus calamus L. and during experiment period, the average removal efficiency of V⁵⁺, Cr⁶⁺, and Cd²⁺ was 52.4, 46.8, and 90.0%, respectively. Combination C (the quality ratio of Acorus calamus L., Phragmites communis Trin., and Alternanthera philoxeroides (Mart.) Griseb is 2:1:1) had the highest removal efficiency on compound heavy metals among three groups and the average removal efficiency of V⁵⁺, Cr⁶⁺, and Cd²⁺ was 18.0, 70.0, and 95.1%, respectively. The highest efficiency of combination C on V⁵⁺ removal was lower than single Alternanthera philoxeroides (Mart.) Griseb group; this may be an existing antagonism in different plants. Heavy metals of V⁵⁺, Cr⁶⁺, and Cd²⁺ had an obviously positive effect on SOD, CAT, and POD of emergent macrophytes. From these results, we conclude that in a phytoremediation for the removal of compound heavy metals where V was dominated pollution in water, the use of Acorus calamus L. species rather than a mixture of several plants should be suggested. When heavy metal pollution was dominated by Cr and Cd, group C rather than a single plant species should be used.

In this study, honey bees (Apis mellifera L.) were exposed to LD₀₅ and LD₅₀ doses of five commonly used acaricides for controlling the parasitic mite, Varroa destructor. LD₅₀ values at 48 h post-treatment showed that tau-fluvalinate was the most toxic, followed by amitraz, coumaphos, thymol, and formic acid. However, the hazard ratios, which estimate the hive risk level based on a ratio of a standard dose of acaricide per hive to the LD₅₀ of the acaricide, revealed that tau-fluvalinate was the most hazardous followed by formic acid, coumaphos, amitraz, and thymol. The expression of the honey bee acetylcholinesterase gene increased after treatment with the LD₀₅ and LD₅₀ acaricide doses and could distinguish three patterns in the timing and level of increased expression between acaricides: one for amitraz, one for tau-fluvalinate and formic acid, and one for coumaphos and thymol. Conversely, changes in cytochrome P450 gene expression could also be detected in response to all five acaricides, but there were no significant differences between them. Changes in vitellogenin gene expression could only detect the effects of tau-fluvalinate, amitraz, or coumaphos treatment, which were not significantly different from each other. Among the acaricides tested, coumaphos, amitraz, and thymol appear to be the safest acaricides based on their hazard ratios, and a good marker to detect differences between the effects of sub-lethal doses of acaricides is monitoring changes in acetylcholinesterase gene expression.

Some tetrahydroisoindoline-1,3-dione derivatives (TDDs) possess potent herbicidal activity. To assess possible impacts of TDDs on humans, the interactions between TDDs and human serum albumin (HSA) were evaluated with steady-state and time-resolved fluorescence spectroscopy, synchronous fluorescence spectroscopy, Fourier transform-infrared spectroscopy, and circular dichroism spectroscopy. The thermodynamic data obtained at temperatures of 298, 307, and 316 K indicate that TDDs spontaneously bind to HSA and thus form a TDD-HSA complex. The conformation and secondary structure of HSA are changed, and the intrinsic fluorescence of HSA is statically quenched by TDDs. Moreover, the TDD-HSA complex is formed primarily through electrostatic interactions and has only one binding site on HSA. A competitive ligand-binding assay revealed that site II (subdomain IIIA) displays the greatest affinity for TDDs. In addition, an acute toxicity bioassay showed no zebrafish mortality upon exposure to 4000 μg L⁻¹ of TDDs. This work is helpful for understanding interactions between TDDs and HSA.

Chlorophyll-a (Chla) is an important indicator of water quality and eutrophication status. Monitoring Chla concentration (C Cₕₗₐ) and understanding the interactions between C Cₕₗₐ and related environmental factors (hydrological and meteorological conditions, nutrients enrichment, etc.) are necessary for assessing and managing water quality and eutrophication. An acceptable Landsat 8 OLI-based empirical algorithm for C Cₕₗₐ has been developed and validated, with a mean absolute percentage error of 14.05% and a root mean square error of 1.10 μg L⁻¹. A time series of remotely estimated C Cₕₗₐ was developed from 2013 to 2015 and examined the relationship of C Cₕₗₐ to inflow rate, rainfall, temperature, and sunshine duration. Spatially, C Cₕₗₐ values in the riverine zone were higher than in the transition and lacustrine zones. Temporally, mean C Cₕₗₐ value were ranked as spring > summer > autumn > winter. A significant positive correlation [Pearson correlation coefficient (r) = 0.88, p < 0.001] was observed between the inflow rate and mean C Cₕₗₐ in the northwest segment of the Xin’anjiang Reservoir. However, no significant relation was observed between mean C Cₕₗₐ and meteorological conditions. Mean (± standard deviation) value for the ratio of total nitrogen concentration to total phosphorus concentration in our in situ dataset is 75.75 ± 55.72. This result supports that phosphorus is the restrictive factor to algal growth in Xin’anjiang Reservoir. In addition, the response of nutrients to Chla has spatial variabilities. Current results show the potential of Landsat 8 OLI data for estimating Chla in slight turbid reservoir and indicate that external pollution loading is an important driving force for the Chla spatiotemporal variability.