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Effect of nitrite addition on the two-phase anaerobic digestion of waste activated sludge: Optimization of the acidogenic phase and influence mechanisms Texto completo
2020
To simultaneously achieve biological denitrification and bio-energy recovery from sludge, the effects of nitrite on the two-phase anaerobic digestion (AD) of waste activated sludge were explored. Herein, effects of nitrite on the acidogenic phase are optimized, and the corresponding influence mechanisms are investigated. The experimental results show that the optimal nitrite treatment conditions for improving the acidogenic phase are an initial pH of 8.0, a nitrite addition concentration of 500 mg NO₂⁻-N·L⁻¹, and a fermentation time of six days. By comparing the effects of nitrite and nitrate on the acidogenic phase, it was found that it was the nitrite, not the nitrate, that significantly enhanced the sludge organic solubilization, hydrolysis, and acidification, which are primarily attributed to the redox property of nitrite. Based on an analysis of different forms of soluble nitrogen concentrations, there was no obvious accumulation of nitrite or nitrate during the acidogenic phase. An analysis of the methane production and the volatile solid (VS) degradation during the two-phase AD revealed that the nitrite improved the methane production from the methanogenic phase and enhanced the VS degradation of sludge during the entire two-phase AD process. These findings could provide references for simultaneously treating nitrite-rich wastewater and improving anaerobic sludge digestion via two-phase system.
Mostrar más [+] Menos [-]Exposure to Benzo[a]pyrene impairs the corpus luteum vascular network in rats during early pregnancy Texto completo
2020
Liu, Min | Deng, Ting | He, Junlin | Ding, Yubin | Liu, Xueqing | Xu, Hanting | Gao, Rufei | Mu, Xinyi | Geng, Yanqing | Liu, Taihang | Wang, Yingxiong | Chen, Xuemei
Benzo [a]pyrene (BaP) is a well-known endocrine disruptor. Exposure to BaP is known to impair embryo implantation. The corpus luteum (CL), the primary source of progesterone during early pregnancy, plays a pivotal role in embryo implantation and pregnancy maintenance. The inappropriate luteal function may result in implantation failure and spontaneous abortions. However, the effect of BaP on CL remains unknown. This study investigated the deleterious effects of BaP on the structure and function of CL during early pregnancy. Pregnant rats were dosed with BaP at 0.2 mg.kg-1. d from day 1 (D1) to day 9 (D9) of gestation. We found that BaP reduced the number of CLs, disturbed the secretion of steroid and impacted the luteal vascular networks. BaP significantly decreased the angiogenesis factor (VEGFR, Ang-1 and Tie2) and increased the anti-angiogenic factor THBS1. Inhibited THBS1 function by LSKL partially rescued the angiogenesis defect caused by BaP. In vitro, BaP metabolite BPDE also interfered the expression levels of angiogenesis-related factors in HUVECs and impaired the angiogenesis, whereas supplemented with rAng-1 can alleviate the anti-angiogenic effect of BPDE. Furthermore, Notch signaling molecules, including Notch1, Dll4, Jag1 and Hey2, which are essential for the establishment and maturation of vascular networks, were affected by BaP exposure. Collectively, BaP broke the molecular regulatory balance between luteal angiogenesis and vascular maturation, impaired the construction of luteal vascular networks, and further affected luteal formation and endocrine function during early pregnancy. Our findings might provide new insight into the relationship between BaP and luteal insufficiency in early pregnancy. These data also give a new line of evidence for curtailing BaP emissions and protecting the women of childbearing age from occupational exposure.
Mostrar más [+] Menos [-]Effect of northern boreal forest fires on PAH fluctuations across the arctic Texto completo
2020
Luo, Jinmu | Han, Yunman | Zhao, Yuan | Huang, Yufei | Liu, Xinrui | Tao, Shu | Liu, Junfeng | Huang, Tao | Wang, Linfei | Chen, Kaijie | Ma, Jianmin
Polycyclic aromatic hydrocarbons (PAHs) are formed by the incomplete combustion of fossil fuels and forest or biomass burning. PAHs undergo long-range atmospheric transport, as evidenced by in situ observations across the Arctic. However, monitored atmospheric concentrations of PAHs indicate that ambient PAH levels in the Arctic do not follow the declining trend of worldwide anthropogenic PAH emissions since the 2000s, suggesting missing sources of PAHs in the Arctic or other places across the Northern Hemisphere. To trace origins and causes for the increasing trend of PAHs in the Arctic, the present study reconstructed PAH emissions from forest fires in the northern boreal forest derived by combining forest carbon stocks and MODIS burned area. We examined the statistical relationships of forest biomass, MODIS burned area, emission factors, and combustion efficiency with different PAH congeners. These relationships were then employed to construct PAH emission inventories from forest biomass burning. We show that for some PAH congeners, for example, benzo[a]pyrene (BaP)—the forest-fire-induced air emissions are almost one order of magnitude higher than previous emission inventories in the Arctic. A global-scale atmospheric chemistry model, GEOS-Chem, was used to simulate air concentrations of BaP, a representative PAH congener primarily emitted from biomass burning, and to quantify the response of BaP to wildfires in the northern boreal forest. The results showed that BaP emissions from wildfires across the northern boreal forest region played a significant role in the contamination and interannual fluctuations of BaP in Arctic air. A source-tagging technique was applied in tracking the origins of BaP pollution from different northern boreal forest regions. We also show that the response of BaP pollution at different Arctic monitoring sites depends on the intensity of human activities.
Mostrar más [+] Menos [-]High levels of antibiotic resistance genes and opportunistic pathogenic bacteria indicators in urban wild bird feces Texto completo
2020
Zhao, Huiru | Sun, Ruonan | Yu, Pingfeng | Alvarez, Pedro J.J.
This study analyzed fresh feces from three common bird species that live in urban environments and interact with human communities. Antibiotic resistance genes (ARGs) encoding resistance to three major classes of antibiotics (i.e., tetracyclines, β-lactams, and sulfonamides) and the mobile genetic element integrase gene (intI1) were abundant (up to 10⁹, 10⁸, 10⁹, and 10¹⁰ copies/g dry feces for tetW, blaTEM, sul1, and intI1, respectively), with relative concentrations surprisingly comparable to that in poultry and livestock that are occasionally fed antibiotics. Biomarkers for opportunistic pathogens were also abundant (up to 10⁷ copies/g dry feces) and the dominant isolates (i.e., Enterococcus spp. and Pseudomonas aeruginosa) harbored both ARGs and virulence genes. ARGs in bird feces followed first-order attenuation with half-lives ranging from 1.3 to 11.1 days in impacted soil. Although residual antibiotics were detected in the feces, no significant correlation was observed between fecal antibiotic concentrations and ARG relative abundance. Thus, other unaccounted factors likely contributed selective pressure for ARG maintenance. These findings highlight the contribution of wild urban bird feces to the maintenance and dissemination of ARGs, and the associated health risks.
Mostrar más [+] Menos [-]Bifenthrin impairs the functions of Leydig and Sertoli cells in mice via mitochondrion-endoplasmic reticulum dysregulation Texto completo
2020
Ham, Jiyeon | You, Seungkwon | Lim, Whasun | Song, Gwonhwa
Bifenthrin (BF) is a synthetic insecticide that is widely used in fields, resulting in an increase in its exposure to animals. However, reports on the toxic effects of BF on mammalian species and the underlying mechanism are still lacking. Here, we elucidated the mechanism underlying the toxic effects of BF on mouse reproduction using cell lines of immature mouse Leydig (TM3) and Sertoli (TM4) cells, which are constituent cells of testes. Our results show that BF suppressed the proliferation and viability of TM3 and TM4 cells. Additionally, treatment with BF induced cell cycle arrest, apoptotic cell death, and DNA fragmentation. Mitochondrial dysfunction and disruption of calcium homeostasis were observed in BF-treated TM3 and TM4 cells. Further, bifenthrin modulated unfolded protein response and mitochondrion-associated membrane and mitogen-activated protein kinase (MAPK)/phosphoinositide 3-kinase (PI3K) signaling pathways. The expression of the mRNAs related to cell cycle progression, steroidogenesis, and spermatogenesis was downregulated by BF, suggestive of testicular toxicity. Taken together, these results demonstrate the intracellular mechanism of action of BF to involve antiproliferative and apoptotic effects and testicular dysfunction in mouse testis.
Mostrar más [+] Menos [-]Benefit-risk associated with the consumption of fish bycatch from tropical tuna fisheries Texto completo
2020
Sardenne, Fany | Lamboy, Nathalie Bodin | Médieu, Anaïs | Antha, Marisa | Arrisol, Rona | Le Grand, Fabienne | Bideau, Antoine | Munaron, Jean-Marie | Le Loc’h, François | Chassot, Emmanuel
Benefit-risk associated with the consumption of fish bycatch from tropical tuna fisheries Texto completo
2020
Sardenne, Fany | Lamboy, Nathalie Bodin | Médieu, Anaïs | Antha, Marisa | Arrisol, Rona | Le Grand, Fabienne | Bideau, Antoine | Munaron, Jean-Marie | Le Loc’h, François | Chassot, Emmanuel
Mercury, omega-3 (docosahexaenoic acid, DHA and eicosapentaenoic acid, EPA) and macronutrients (fat and proteins) were quantified on a wet weight (ww) basis in 20 species of fish taken as bycatch in tropical tuna fisheries. Based on a hazard quotient taking into account mercury and omega-3 contents, a benefit-risk assessment for the consumption of these pelagic species was conducted for three people categories: young children, children and adults. All fish bycatch were found to be an excellent source of proteins (min‒max = 14.4–25.2 g/100 g fillet), had low omega-6/omega-3 ratios (<1, except for silky shark), and had mercury content below the safety limits defined by sanitary agencies. Silky shark and Istiophoridae had the highest mercury contents (min‒max = 0.029–0.317 ppm ww). Omega-3 contents were the lowest in silky shark (0.2 ± 0.2 mg/100 g fillet) and the highest in striped marlin (3.6 ± 3.2 g/100 g fillet). Billfishes (Istiophoridae, including striped marlin), minor tunas (Scombridae), and Carangidae had the highest omega-3 contents (min‒max = 0.68–7.28 g/100 g fillet). The highest hazard quotient values obtained for silky shark and great barracuda reflected a lower nutritional benefit (i.e., low omega-3 source) than risk (i.e., mercury exposure), making them not advisable for consumption. Eight species had low hazard quotients, and among them cottonmouth jack and flat needlefish were found of high health interest (high protein, moderate fat contents, and low omega-6/omega-3 ratio). A daily serving portion of 85–200 g (according to people category) can be recommended for these species. Batfish, and to a lower extent pompano dolphinfish and brassy chub, can also be consumed safely and would provide greater health benefits than risks. These results advocate for a better access of these species to local populations.
Mostrar más [+] Menos [-]Benefit-risk associated with the consumption of fish bycatch from tropical tuna fisheries Texto completo
2020
Sardenne, Fany | Bodin, Nathalie | Médieu, Anais | Antha, Marisa | Arrisol, Rona | Le Grand, Fabienne | Bideau, Antoine | Munaron, Jean-marie | Le Loc’h, François | Chassot, Emmanuel
Mercury, omega-3 (docosahexaenoic acid, DHA and eicosapentaenoic acid, EPA) and macronutrients (fat and proteins) were quantified on a wet weight (ww) basis in 20 species of fish taken as bycatch in tropical tuna fisheries. Based on a hazard quotient taking into account mercury and omega-3 contents, a benefit-risk assessment for the consumption of these pelagic species was conducted for three people categories: young children, children and adults. All fish bycatch were found to be an excellent source of proteins (min‒max = 14.4‒25.2 g/100g fillet), had low omega-6/omega-3 ratios (<1, except for silky shark), and had mercury content below the safety limits defined by sanitary agencies. Silky shark and Istiophoridae had the highest mercury contents (min‒max = 0.029‒0.317 ppm ww). Omega-3 contents were the lowest in silky shark (0.2±0.2 mg/100g fillet) and the highest in striped marlin (3.6±3.2 g/100g fillet). Billfishes (Istiophoridae, including striped marlin), minor tunas (Scombridae), and Carangidae had the highest omega-3 contents (min‒max = 0.68‒7.28 g/100g fillet). The highest hazard quotient values obtained for silky shark and great barracuda reflected a lower nutritional benefit (i.e., low omega-3 source) than risk (i.e., mercury exposure), making them not advisable for consumption. Eight species had low hazard quotients, and among them cottonmouth jack and flat needlefish were found of high health interest (high protein, moderate fat contents, and low omega-6/omega-3 ratio). A daily serving portion of 85‒200 g (according to people category) can be recommended for these species. Batfish, and to a lower extent pompano dolphinfish and brassy chub, can also be consumed safely and would provide greater health benefits than risks. These results advocate for a better access of these species to local populations.
Mostrar más [+] Menos [-]Mechanistic insights and multiple characterizations of cadmium binding to animal-derived biochar Texto completo
2020
Lei, Sicong | Zhu, Ling | Xue, Cong | Hong, Chengyi | Wang, Junliang | Che, Lei | Hu, Yongfeng | Qiu, Yuping
Cattle-derived biochar (CB), which is derived from industrial pyrolysis of cattle carcasses in harmless treatment plants, is a naturally occurring mineral form of carbonate-bearing hydroxyapatite (CHAP) with a small amount of elemental carbon. CB has 4.02% of carbonate content, which falls under the B-type substitution of CHAP. In this work, the Cd(II) sorption capacity of CB was determined to be 0.82 mmol/g, with 97.6% of the Cd(II) uptake contributing to CHAP and only 2.36% of the Cd(II) uptake contributing to the elemental carbon component. The calculation and linear combination fitting (LCF) of Cd L₃-edge X-ray absorption near-edge structure (XANES) analysis indicated that the contributions of Cd(II) species to CB presented the following order: ion exchange (57.6%–61.0%) > precipitation (24.4%–29.9%) > surface complexation (12.5%–13.4%). The depth dependent X-ray photoelectron spectroscopy (XPS) showed the presence of ion exchange, which is accompanied by intraparticle diffusion. LCF of XANES and Rietveld analysis of X-ray diffraction (XRD) demonstrated that Cd(II) was precipitated in the form of Cd₅H₂(PO₄)₄·4H₂O on the CB surface. Furthermore, the precipitate was directly observed and identified by scanning electron microscopy with energy-dispersive spectroscopy (SEM-EDS). Consequently, we revealed the intricate binding mechanism of Cd(II) to CHAP-rich CB and confirmed the importance of surface precipitation.
Mostrar más [+] Menos [-]Investigating arsenic impact of ACC treated timbers in compost production (A case study in Christchurch, New Zealand) Texto completo
2020
Safa, Majeed | O’Carroll, Daniel | Mansouri, Nazanin | Robinson, Brett | Curline, Greg
The arsenic concentration is an important issue in compost production. The main inputs of a compost factory, including kerbsides, green wastes, food industry wastes, and river weeds are investigated in this study. Also, this study investigated how treated timbers, ashes, and other contamination can impact arsenic concentration in compost production. The results showed that most treated timbers and all ashes of treated and untreated timbers contained significant amounts of arsenic. These results revealed that the presence of a small amount of treated timber ashes can significantly increase the arsenic concentration in composts. The results of the study show the arsenic concentration in compost increase during cold months, and it dropped during summer, which would be mostly because of high arsenic concentration in ashes of log burners. This study shows ashes of burning timbers can impact arsenic contamination mostly because of using Copper-Chrome-Arsenic wood preservatives (CCA). Also, the lab results show the arsenic level even in ashes of untreated timber is around 96 ppm. The ashes of H3, H4, and H5 treated timbers contain approximately 133,000, 155,000, and 179,000 ppm of arsenic, which one kg of them can increase arsenic concentration around 10 ppm in 13.3, 15.5 and 17.9 tons of dry compost products. The main problem is many people look at ashes and treated timber as organic materials; however, ashes of treated and untreated timbers contained high concentrations of arsenic. Therefore, it was necessary to warn people about the dangers of putting any ashes in organic waste bins.
Mostrar más [+] Menos [-]The imidacloprid remediation, soil fertility enhancement and microbial community change in soil by Rhodopseudomonas capsulata using effluent as carbon source Texto completo
2020
Wu, Pan | Zhang, Xuewei | Niu, Tong | Wang, Yanling | Liu, Rijia | Zhang, Ying
The effects of Rhodopseudomonas capsulata (R. capsulata) in the treated effluent of soybean processing wastewater (SPW) on the remediation of imidacloprid in soil, soil fertility, and the microbial community structure in soil were studied. Compared with the control group, with the addition of effluent containing R. capsulata, imidacloprid was effectively removed, soil fertility was enhanced, and the microbial community structure was improved. Molecular analysis indicated that imidacloprid could exert induction effects on expression of cpm gene and regulation effects on the synthesis of cytochrome P450 monooxygenases (P450) by activating HKs gene in two-component system (TCS). For R. capsulata, this induction process required 1 day. The synthesis of P450 occurred 1 day after inoculation, because R. capsulata are a type of archaea and imidacloprid is an environmental stress. Before expression of the cpm gene and synthesis of P450, R. capsulata need a period of time to adapt to external imidacloprid stimulation. However, the lack of organic matter in the soil cannot sustain R. capsulata growth for more than 1 day. In four groups with added effluent, the remaining organic matter in the effluent provided a sufficient carbon source and energy for R. capsulata. Five days later, the microbial community structure was improved by R. capsulata in the soil. The new technique could be used to remediate imidacloprid, enhance soil fertility, treat SPW and realize the recycling and reuse of wastewater and R. capsulata cells.
Mostrar más [+] Menos [-]Genome-wide DNA methylation analysis reveals significant impact of long-term ambient air pollution exposure on biological functions related to mitochondria and immune response Texto completo
2020
Wang, Maiqiu | Zhao, Junsheng | Wang, Yan | Mao, Ying | Chao, Shin-Yi | Huang, Peng | Liu, Qiang | Ma, Yunlong | Yao, Yinghao | Yang, Zhongli | Yuan, Wenji | Cui, Wenyan | Payne, Thomas J. | Li, Ming D.
Exposure to long-term ambient air pollution is believed to have adverse effects on human health. However, the mechanisms underlying these impacts are poorly understood. DNA methylation, a crucial epigenetic modification, is susceptible to environmental factors and likely involved in these processes. We conducted a whole-genome bisulfite sequencing study on 120 participants from a highly polluted region (HPR) and a less polluted region (LPR) in China, where the HPR had much higher concentrations of five air pollutants (PM₂.₅, PM₁₀, SO₂, NO₂, and CO) (fold difference 1.6 to 6.6 times; P value 1.80E-07 to 3.19E-23). Genome-wide methylation analysis revealed 371 DMRs in subjects from the two areas and these DMRs were located primarily in gene regulatory elements such as promoters and enhancers. Gene enrichment analysis showed that DMR-related genes were significantly enriched in diseases related to pulmonary disorders and cancers and in biological processes related to mitochondrial assembly and cytokine production. Further, HPR participants showed a higher mtDNA copy number. Of those identified DMRs, 15 were significantly correlated with mtDNA copy number. Finally, cytokine assay indicated that an increased plasma interleukin-5 level was associated with greater air pollution. Taken together, our findings suggest that exposure to long-term ambient air pollution can lead to alterations in DNA methylation whose functions relate to mitochondria and immune responses.
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