Bisphenol A diglycidyl ether (BADGE), a derivative of bisphenol A (BPA), is widely used in the manufacture of epoxy resins as well as a coating on food containers. Recent studies have demonstrated the adverse effects of BADGE on reproduction and development in rodents and amphibians, but how BADGE affects biological activity is not understood. To gain a better understanding of the biological effects of BADGE exposure during development, we used the model organism Drosophila melanogaster and performed whole transcriptome sequencing. Interestingly, when Drosophila are raised on food containing BADGE, genes having significantly increased transcript numbers are enriched for those involved in regulating cell proliferation, including DNA replication and cell cycle control. Furthermore, raising larvae on BADGE-containing food induces hemocyte (blood cell) over-proliferation. This effect can be stimulated with even lower concentrations of BADGE if the hemocytes are already primed for cell proliferation by the expression of dominant active Ras GTPase. We conclude that chronic exposure to the xenobiotic BADGE throughout development can induce cell proliferation.

Hydrogen cyanide (HCN) is volatile and highly toxic with acute and chronic effects on humans. Gaseous HCN enters the atmosphere from natural processes or industrial activities, which lead to human exposure. Effective intervention in cases of HCN inhalation requires an efficient diagnostic tool. The existing physiologically based pharmacokinetic (PBPK) model for HCN cannot clearly simulate continuous HCN inhalation or predict HCN levels in inhaled air. The current study presents a PBPK model for continuous inhalation of HCN, called Human Continuous Cyanide Inhalation Predictor (HCCIP). Since existing data on pharmacokinetics of HCN inhalation are limited, HCCIP utilizes extensive data from the current authors’ PBPK model on cyanide ingestion. The structure of HCCIP comprises the lungs, kidneys, liver, and slowly perfused tissue. In both the human body and in exhaled air, HCCIP features the ability to predict concentration-time courses of cyanide. Moreover, HCCIP can predict HCN concentration in inhaled air from known blood cyanide levels. After completion, the results of HCCIP were validated against preexisting published datasets. The simulation results agreed with these datasets, validating the model. The HCCIP model is an effective tool for assessing risk from continuous HCN inhalation, and HCCIP extends the capabilities of air dispersion modeling, such as AERMOD or CALPUFF, to assess HCN risk from specific release sources.

Nutrient retention is an important process in lake nutrient cycling of lakes and can mitigate lake eutrophication. However, little is known about temporal lake nutrient retention efficiency and it varies due to changes in hydrological, ecological, and nutrient inputs to lake waters. Quantitative information about seasonal lake N and P retention is critical for developing strategies to reduce eutrophication in lake systems. This study investigated TN and TP retention efficiencies and retention masses using water and mass balance calculations, and statistically analyzed the seasonal variability of nutrient retention in Lake Chaohu, China, from 2014 to 2018. Lake Chaohu experienced large amounts of external loads inputs (23.2 g N m⁻² year⁻¹ and 1.3 g P m⁻² year⁻¹), and approximately 58% TN and 48% TP were retained annually. The lake acted more as a sink for N than for P. The mean annual TP retention efficiency decreased (P < 0.05) over the study period, indicating that TP retention capacity was gradually exceeded. Seasonal variability of TN and TP retention efficiency was high and ranged from − 18.7 to 144.1% and from − 58.8 to 170.7%, respectively, over the five study years. The internal P loads over the study period were equivalent to roughly 9% of the total external loads. The annual nutrient retention efficiency of TN and TP increased with hydraulic residence time, while water temperature was an essential factor for the contrasting seasonal variation patterns of TN and TP retention efficiencies.

The efficiency of the proteolytic strain Anoxybacillus kamchatkensis M1V in the fermentation of speckled shrimp by-product was investigated for the recovery of a deproteinized bioactive hydrolysate. The biological activities of the resulting hydrolysate were also examined by applying several antioxidant and enzyme inhibitory assays. The strain M1V was found to produce high level of protease activity (2000 U/mL) when grown in media containing only shrimp powder at 25 g/L. The crude protease displayed a significant deproteinization capabiliy, with the best efficiency (48%) being recorded for an enzyme to substrate (E/S) ratio of 30 U/mg. Following the deproteinization, chitin was recovered and the authenticity was confirmed by Fourier-transform infrared spectroscopy (FTIR) analysis. On the other hand, the obtained hydrolysate showed a significant enzymatic inhibitory potential against acetylcholinesterase, tyrosinase, amylase, and angiotensin I convertase, and a strong antioxidant activity. Graphical Abstract

According to the World Health Organization (WHO), in 2016, 91% of the global population was living in places where guidelines on air quality were not met, which results in an estimated figure of seven million deaths annually. The new Brazilian air quality standards, CONAMA 491/2018, was the first revision in over two decades and has as final target the WHO guidelines for air quality, although no deadline has been established for implementation. The goal of this work was to quantify public health gains of this new policy based on hospitalizations due to respiratory diseases, the most studied outcome in Brazilian time series studies, in four Brazilian Southeast capitals: São Paulo (SP), Rio de Janeiro (RJ), Belo Horizonte (MG), and Vitória (ES) for PM₁₀, PM₂,₅, SO₂, CO, and O₃. Population and hospitalizations data for all respiratory diseases for people under 5 years old, over 64 years old, most vulnerable populations, and all ages were analyzed. The air quality monitoring data was analyzed in two different periods: 2016 to 2018 for São Paulo and Vitória; and between 2015 and 2017 for Belo Horizonte and Rio de Janeiro, according to available monitoring data. A literature review was carried out to determine the appropriate relative risk to be used in the estimations, and the public health gains were calculated based on the selected relative risks for each city. The highest estimate was for São Paulo, with 3454 avoidable respiratory hospital admissions (all ages). In total, the four cities accounted for 4148 avoidable hospitalizations, which was associated to $1.1 million public health gains. Results considering the day of exposure (lag 0) were superior to those with the 5-day moving average (lag 5). The results highlighted the importance of adopting more restrictive standards and called for public policies, the necessity of expanding the air quality monitoring network, mapping emission sources, and improve the knowledge about the interaction between air pollution and health outcomes beyond respiratory disease for the region.

Cadmium (Cd) is a heavy metal known to be harmful to human health. The aim of this study was to analyze the regional characteristics of dietary Cd intake of adult male residents in different regions of China. The value of this research is to guide regional consumers to minimize Cd intake by adjusting diets for different food categories in various regions. Dietary Cd intake data is used from the 4th and 5th Chinese Total Diet Studies (CTDS). The 4th CTDS collected data from 12 provinces, covering about 50% of the Chinese population. The 5th CTDS collected data from 20 provinces, covering about 67% of the Chinese population. Over 200 kinds of foods were sampled and divided into 13 categories and organized by geographic information system (GIS) mapping. The results showed that firstly, the dietary Cd intake of Chinese residents has increased; secondly, the food groups with the highest Cd intake were cereals and vegetables; and thirdly, the main food group of dietary Cd intake in different regions is presented such as cereals in Sichuan, Jiangxi, Zhejiang, Guangdong, Fujian, and Guangxi and vegetables in Shanghai, Ningxia, and Heilongjiang. Lastly, the food product with the highest dietary Cd intake concentration was found to be nori in 5 provinces. The other notable foods, with Cd intake, included peanuts, rice, squid, cuttlefish, and mushrooms.

The invasive and widespread golden apple snail (GAS, Pomacea canaliculata) is a harmful crop pest in many parts of Asia. The heavy use of molluscicides to control GAS could result in soil and water pollution as well as in loss of biodiversity. A sustainable and pollution-free control method is urgently needed to counteract this invasion. In this study, we proposed using dried and powdered GAS residue to neutralize and fertilize soils. We compared the effects of adding GAS residue (i.e., ground GAS shell and meat residue) to the effects of adding lime upon soil properties and microbes in a greenhouse pot experiment. Each pot was incubated for 120 days, and soil pH, nutrients, microbial species, and enzyme activity were assessed. Results showed that addition of GAS residue significantly improved soil pH, contents of total organic carbon (TOC), total nitrogen (TN), total phosphorus (TP), and available nitrogen but decreased soil available phosphorus (AP) content due to phosphorus sorption induced by soil organic matter (OM) and high pH. The GAS residue added to soil released nutrients and alleviated soil acidity, as well as provided more resources to soil microbes to increase their bioactivity, although lime addition was better at mitigating soil acidity. We found that with added GAS residue of 25 g kg⁻¹, the soil nitrate nitrogen (NO₃-N) content increased by 10 times; microbial biomass increased by 43%; and enzyme activity of β-1,4-glucosidase, β-1,4-N-acetylglucosaminidase, and β-D-cellobiosidase also were enhanced, compared to the control. Our findings suggest that GAS residue functions well as a fertilizer and soil amendment to aid the remediation of barren and acidic soils, making it a valuable and useful option in the control of the invasive GAS.

A combined amendment (CF) consisting of 90% calcium sulfate (CaSO₄) and 10% ferric oxide (Fe₂O₃) was used to investigate the feasibility, active principles, and possible mechanisms of the immobilization of heavy metals in paddy soil. A soil incubation experiment, two consecutive pot trials, and a field experiment were conducted to evaluate the effectiveness and persistence of CF on metal(loid) immobilization. Soil incubation experiment results indicated that the application of CF significantly decreased the concentrations of cadmium (Cd), lead (Pb), and arsenic (As) in soil solution. CF treatments simultaneously reduced the accumulation of Cd, Pb, and As in two consecutive pot trials. The total Cd, Pb, and As concentrations in the rice grains were respectively 0.02, 2.08, and 0.62 mg kg⁻¹ in the control treatment in the second year, which exceeded the safety limits of contaminants in food products in China. However, a high amount of CF amendment (CF-H, 0.3%) effectively decreased Cd, Pb, and As by 75.0%, 75.5%, and 46.8%, respectively. Further, with the CF amendment, the bioavailable Cd and Pb in the soil and the accumulation of Cd, Pb, and As in rice grain in the field experiment were also significantly decreased. The concentrations of Cd, Pb, and As in grains were respectively 0.02, 0.03, and 0.39 mg kg⁻¹ in the control treatment in the field experiment, which decreased to 0.01, 0.01, and 0.22 mg kg⁻¹ with CF addition, suggesting that grains produced in the field could pose less health risk. In conclusion, these results implied that CF was an effective and persistent combined amendment to immobilize heavy metals in soil and thereby can reduce the exposure risk of metal(loid)s associated with rice consumption.

Wastes from the Pacara Earpod tree (Enterolobium contortisilquum) and Ironwood (Caesalpinia leiostachya) seeds were studied as biosorbents for the removal of basic fuchsin from waters. Both biosorbents were prepared and characterized by different analytical methods. The characterization data showed that both materials were mainly composed of lignin, cellulose, and hemicellulose. Both biosorbents exhibited roughened surfaces and surface functional groups such as C-H, C=O, C=C, C-O, C-N, and OH bonds. Furthermore, the XRD pattern shows an amorphous phase with a wide peak from 10 to 30° due to the lignin. In terms of dosage and pH, the use of 1 g L⁻¹ and 9.0, respectively, is recommended. The initial concentrations for the biosorption kinetics ranged from 50 to 500 mg L⁻¹, where the Pacara ear and the Ironwood reached an adsorption capacity of 145.62 and 100.743 mg g⁻¹ for the 500 mg L⁻¹. The pseudo-second-order was found to be the proper model for describing biosorption of basic fuchsin onto Pacara Earpod tree and Ironwood, respectively. For the isotherm experiments, the maximum experimental biosorption capacity was found to be 166.858 and 110.317 mg g⁻¹ for the Pacara Earpod and Ironwood for the initial concentration of 500 mg L⁻¹ at 328 K. The Langmuir and the Tóth models were the best for representing the equilibrium curves for the basic fuchsin on the Pacara Earpod and the Ironwood, respectively. Maximum adsorption capacities of 177.084 mg g⁻¹ and 136.526 mg g⁻¹ were achieved for the Pacara Earpod tree and Ironwood, respectively. The biosorption process was spontaneous, endothermic, and favorable for both biosorbents. The biosorbents were also applied for coloration removal of simulated textile effluents, reaching 66% and 54% for the Pacara Earpod and Ironwood, respectively. For the final application, the materials were used in fixed-bed biosorption, with an initial concentration of 200 mg L⁻¹, reaching breakthrough times of 710 and 415 min, leading to biosorption capacities of the column of 124.5 and 76.5 mg g⁻¹, for the Pacara Earpod and Ironwood, respectively.

The aim of this review paper is to critically analyze the existing studies on waste electric and electronic equipment (WEEE), which is one of the most increasing solid waste streams. This complex solid waste stream has pushed many scientific communities to develop novel technologies with minimum ecological disturbance. Noteworthy amount of valuable metals makes e-waste to a core of “urban mining”; therefore, it warrants special attention. Present study is focused on all the basic conceptual knowledge of WEEE ranging from compositional analysis, global statistics of e-waste generation, and metallurgical processes applied for metals extraction from e-waste. This review critically analyses the existing studies to emphasize on the heterogeneity nature of e-waste, which has not been focused much in any of the existing review articles. Comprehensive analysis of conventional approaches such as pyrometallurgy and hydrometallurgy reveals that high costs and secondary pollution possibilities limit the industrial feasibilities of these processes. Therefore biohydrometallurgy, a green technology, has been attracting researchers to focus on this novel technique to implement it for metal extraction from WEEE.